BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0195.p (338 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313077|ref|YP_003777169.1| coenzyme F420-dependent N5,N10-... 545 e-156 gi|300312181|ref|YP_003776273.1| coenzyme F420-dependent N5,N10-... 68 1e-12 >gi|300313077|ref|YP_003777169.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 336 Score = 545 bits (1403), Expect = e-156, Method: Compositional matrix adjust. Identities = 261/334 (78%), Positives = 297/334 (88%), Gaps = 1/334 (0%) Query: 1 MIPFSVLDLSPINQGSTAAQAFNNSKELAQKAEALGYNRYWLAEHHNMTGIASAATSVVI 60 MIPFSVLDLSPINQGSTAAQAFNNSKELAQ+AEALGY+RYWLAEHHNM GIASAAT++VI Sbjct: 1 MIPFSVLDLSPINQGSTAAQAFNNSKELAQRAEALGYHRYWLAEHHNMPGIASAATALVI 60 Query: 61 SHIAANTKTIRVGAGGIMLPNHSPLVIAEQFGTLESLYPGRIDLGLGRAPGSDQRTARAL 120 SH+AANT++IRVG+GGIMLPNHSPLVIAEQFGTLESLYPGRIDLGLGRAPGSDQRTARAL Sbjct: 61 SHVAANTRSIRVGSGGIMLPNHSPLVIAEQFGTLESLYPGRIDLGLGRAPGSDQRTARAL 120 Query: 121 RRHLG-DSADTFPQDVVELQEYFKEASPYQSVRAVPGYGLNVPLWLLGSSMFSAQLAAEM 179 RRH+G D+ADTFPQDV ELQ+YF + + YQ++RAVPG GL VP+WLLGSSMFSAQLAAE+ Sbjct: 121 RRHMGGDTADTFPQDVQELQDYFDDPADYQTLRAVPGAGLKVPIWLLGSSMFSAQLAAEL 180 Query: 180 GLPFAFASHFAPGFMKSAVEIYRSRFKPSDALKRPHVMLGLNVFAAETDREGERLFTSLQ 239 GLPFAFASHFAPGFMKSAV+IYR RFKPS LK+P+VML LNVFAA+T RE ERLFTSLQ Sbjct: 181 GLPFAFASHFAPGFMKSAVQIYRERFKPSATLKKPYVMLALNVFAADTQREAERLFTSLQ 240 Query: 240 QQFVNLVRGVPGQLNPPRESMEDYWRPEEKSHVERSLSCAIVGSPETVRTGLQNFIDDTG 299 QQF+NL+RG PGQL PP + ++ W+P E++HV RSL+CA+VG + V+ GLQ F+D+ Sbjct: 241 QQFMNLIRGTPGQLRPPVDDIDALWQPGEQAHVARSLACAVVGDRQAVQDGLQAFMDEHA 300 Query: 300 ADELMITAQIHDHAARLRSFEIVAEARAALTPKV 333 DEL+ITAQI DHAARL SFEI AEAR AL V Sbjct: 301 PDELIITAQIFDHAARLHSFEIAAEARQALREPV 334 >gi|300312181|ref|YP_003776273.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 67.8 bits (164), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 37/96 (38%), Positives = 54/96 (56%) Query: 13 NQGSTAAQAFNNSKELAQKAEALGYNRYWLAEHHNMTGIASAATSVVISHIAANTKTIRV 72 N A A + + A AEALGY+ W+AEHH + +A V+++ IAA T IR+ Sbjct: 16 NPHGNARDALLQTVDHAVVAEALGYHDVWVAEHHYSSFAIGSALMVLLAQIAARTSRIRL 75 Query: 73 GAGGIMLPNHSPLVIAEQFGTLESLYPGRIDLGLGR 108 G G +L + PL +AE TL+ L GR++ G+ R Sbjct: 76 GTGASLLALNDPLRVAEDVATLDLLSNGRMEFGVAR 111 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,077,769 Number of extensions: 45508 Number of successful extensions: 136 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 135 Number of HSP's successfully gapped: 2 Length of query: 338 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 252 Effective length of database: 1,212,660 Effective search space: 305590320 Effective search space used: 305590320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)