BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0194.p (695 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313074|ref|YP_003777166.1| GGDEF domain-containing protein... 866 0.0 gi|300311453|ref|YP_003775545.1| signal transduction containing ... 159 6e-40 gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 147 3e-36 gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 146 6e-36 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 145 1e-35 >gi|300313074|ref|YP_003777166.1| GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 738 Score = 866 bits (2238), Expect = 0.0, Method: Compositional matrix adjust. Identities = 429/677 (63%), Positives = 534/677 (78%), Gaps = 4/677 (0%) Query: 14 LVDQFTRSYARHEAEARLNQLAWQMQDALDRSMSERYIDVKQLSQRPSIREAKDPARMRA 73 LVDQFTR YAR E E RL QLAWQMQDAL+R M+ER ID++ L RPSIR+A+DPA +RA Sbjct: 56 LVDQFTRGYARSEVETRLKQLAWQMQDALERGMAEREIDIRLLVSRPSIRQAQDPAHIRA 115 Query: 74 VFNELQRNVPNYAWIGFATPDGNVLAAANGLLEGQSVAQRPWYQKAQKDFFVGDYHPAVL 133 V ELQRNVP+YAWIG A PDG VLAA GLLEG SVA+RPW+Q+ Q+ + DYHPAVL Sbjct: 116 VLEELQRNVPHYAWIGMARPDGTVLAATKGLLEGMSVAERPWFQQGQQTEYAADYHPAVL 175 Query: 134 LEKKLPVMTDPWRFVDISTPIMGPDGQFQGVLGVHLSWTWARDLAKKLLNPANDRYDVEI 193 LEKKLP +DPWRFVD+S P++ GQ GV+G+HLSW WAR++A KLLNP + RYDV++ Sbjct: 176 LEKKLPPQSDPWRFVDLSMPVLNMQGQMIGVMGMHLSWGWAREIASKLLNPTSSRYDVDV 235 Query: 194 MIVREDGTVLLGPRELEEKKIASDSFKASLHLPSG-SMRELGEDGQHYLTGFARTGLSSE 252 +IVR DGTV+LGP+++EE KI + SF+A+ H +G S+ E DG+ Y+TG+ARTG E Sbjct: 236 LIVRSDGTVILGPKDMEETKIDTASFRAAQHSSAGGSLIETWPDGRRYVTGYARTGQGGE 295 Query: 253 YTEMKWSVLVRQPEQVAMSGFKDLERQVLFVGCIVGLLLMALAFFMTRRLVAPMNALSAA 312 ++KW+V+VRQPE VA+ F+DLER+V+ VG + G +L+ LAF +TRRLVAP+NALSAA Sbjct: 296 M-KLKWTVIVRQPETVALEAFRDLERRVIVVGALAGAVLVLLAFMLTRRLVAPINALSAA 354 Query: 313 LERRATGDHSAEIPIVGTYYEIQLLSTTLSTMVQREENYLREVRSLNEGLEQRVEDRTRR 372 LE+RA G EIP+V + E+QLLS+ L TMV+REE YLRE+RSLNE LE+RV +RT R Sbjct: 355 LEQRAAGLQEVEIPLVTSVRELQLLSSALGTMVEREERYLREMRSLNESLEERVVERTSR 414 Query: 373 LHETAIALQQSLDNQRENQIRLEESETELRAILQNAHDAFIAIDEDGMVLEWNRQAEQLL 432 LHETA ALQQ+LD Q+ NQI+LEES ELRAIL+NA DAFIA DE G ++EWNRQAE LL Sbjct: 415 LHETATALQQALDQQQTNQIQLEESAAELRAILENAQDAFIAADEHGHIVEWNRQAELLL 474 Query: 433 GWAHKEAVGKELAELIIPFEQRELHRRGMIHFLNTGEGMVINNRIEINALRRDGNELPIE 492 GW +G+ +AELIIP + R H GM FL G +VINNRIE+ AL RDG+ELP+E Sbjct: 475 GWPRDTVMGRSMAELIIPSDMRAAHNHGMAQFLKDGTSVVINNRIELQALHRDGHELPVE 534 Query: 493 MTVGYVRRRAGHLFIAFLHDITERQAFRNSLQEMALTDILTGLPNRRAFTEKLPEAMARA 552 +TVG+V+R++GHLFIAFLHDITER AFR+ ++E+ALTD+LT LPNRRAF+++LPE M RA Sbjct: 535 ITVGHVKRQSGHLFIAFLHDITERLAFRDQMRELALTDVLTSLPNRRAFSDRLPEGMMRA 594 Query: 553 IREPQQMGLLFMDVDGFKSINDKYGHEAGDELLVKFAERLIDAVREVDTVARLAGDEFTV 612 R Q + L FMD+DGFK IND+YGHEAGDELLV+FA RL +VR+VDTVARLAGDEF V Sbjct: 595 RRAHQLLALFFMDLDGFKGINDRYGHEAGDELLVEFARRLQASVRDVDTVARLAGDEFVV 654 Query: 613 ILERLHGPADAMGVAEKILVSMRRPFILTAATVNVSTSIGVAIYEP--GQHIDQDALVAQ 670 ILE L DA+ VAEKIL ++R PF LT +TV++S+SIGVA+Y+P I + L++ Sbjct: 655 ILENLQSQQDAIMVAEKILHAIREPFELTHSTVHISSSIGVAVYQPDLDGEIGPEELLSM 714 Query: 671 ADKAMYAAKRAGKNRVE 687 ADKAMYAAKR GKNR+E Sbjct: 715 ADKAMYAAKRLGKNRIE 731 >gi|300311453|ref|YP_003775545.1| signal transduction containing PAS/GGDEF/EAL domains protein [Herbaspirillum seropedicae SmR1] Length = 683 Score = 159 bits (403), Expect = 6e-40, Method: Compositional matrix adjust. Identities = 102/283 (36%), Positives = 150/283 (53%), Gaps = 12/283 (4%) Query: 404 ILQNAHDAFIAIDEDGMVLEWNRQAEQLLGWAHKEAVGKELAELIIPFEQRELHRRGMIH 463 IL H++ I +D G + WNR AEQL G++ EA+G+ + + +E + Sbjct: 127 ILNQMHESVITMDPAGFITSWNRGAEQLFGYSAMEAIGRNV---LFLYENEDEEDSLHDL 183 Query: 464 FLNTGEGMVINNRIEINALRRDGNELPIEMTVGYVRRRAGHL--FIAFLHDITERQAFRN 521 FL G +E+ ++ G +++ +R + GH I +L+DITER+ Sbjct: 184 FLEHG-----GREMEVRRRKKSGEVFWASLSLSVMRDQNGHPIGLIGYLNDITERKNAEK 238 Query: 522 SLQEMALTDILTGLPNRRAFTEKLPEAMARAIREPQQMGLLFMDVDGFKSINDKYGHEAG 581 + +A D LTGLPNR T + +A+ A E ++F+D++ FK IND GH AG Sbjct: 239 LIHHLAYYDALTGLPNRTLLTRTVDQALEEARHENLHGCVMFIDLNRFKPINDTLGHVAG 298 Query: 582 DELLVKFAERLIDAVREVDTVARLAGDEFTVILERLHGPADAMGVAEKILVSMRRPFILT 641 D LLV+ A RL A+RE D VARL DEF V L + + A VA+K+L PF + Sbjct: 299 DMLLVEVAMRLRRALREQDVVARLGADEFAVALFGIDKDSPAY-VAQKLLALFDDPFFID 357 Query: 642 AATVNVSTSIGVAIYEPGQHIDQDALVAQADKAMYAAKRAGKN 684 + V SIGV++Y P D + L+ AD AMY AK+ G+N Sbjct: 358 GHELRVGASIGVSLY-PENATDTETLLRLADIAMYRAKQGGEN 399 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 147 bits (371), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 81/162 (50%), Positives = 106/162 (65%), Gaps = 2/162 (1%) Query: 523 LQEMALTDILTGLPNRRAFTEKLPEAMARAIREPQQMGLLFMDVDGFKSINDKYGHEAGD 582 L +M L D LT LPNR ++L +A+ +A RE L+FMD+DGFK+IND GH GD Sbjct: 262 LTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHFALMFMDLDGFKAINDSLGHHVGD 321 Query: 583 ELLVKFAERLIDAVREVDTVARLAGDEFTVILERLHGPADAMGVAEKILVSMRRPFILTA 642 LLV+ A+RL + R DTVARL GDEF VIL L+ P DAMGVAEK++ ++ +PF + Sbjct: 322 RLLVEVAQRLTSSSRANDTVARLGGDEF-VILVDLNTPEDAMGVAEKLVEAVNQPFKVDQ 380 Query: 643 ATVNVSTSIGVAIYEPGQHIDQDALVAQADKAMYAAKRAGKN 684 + VS S+G+AIY P + LV AD AMY KR+G+N Sbjct: 381 HELRVSASVGIAIY-PEDGATRHDLVINADAAMYHTKRSGRN 421 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 146 bits (368), Expect = 6e-36, Method: Compositional matrix adjust. Identities = 96/298 (32%), Positives = 155/298 (52%), Gaps = 17/298 (5%) Query: 396 ESETELRAILQNAHDAFIAIDEDGMVLEWNRQAEQLLGWAHKEAVGKELAELIIPFEQRE 455 E+E + A+ ++ + + D + +L N+ ++ G++ +E VGK L Sbjct: 471 EAELRISAVAFDSREGMLITDANSKILRVNKAFTEITGYSAEEIVGKHPNLL-------R 523 Query: 456 LHRRGMIHFLNTGEGMVINNRI--EINALRRDGNELPIEMTVGYVRRRAGHL--FIAFLH 511 R G F E + + EI R++G P +T+ V+ + + +++ H Sbjct: 524 SDRHGPAFFQEMRESIRRFGKWQGEIWDRRKNGEVYPEWLTISAVKNKDQQVTHYVSTHH 583 Query: 512 DITERQAFRNSLQEMALTDILTGLPNRRAFTEKLPEAMARAIREPQQMGLLFMDVDGFKS 571 DI++R+ ++E+A D LT LPNR ++L +A+A ++R LLF+D+D FK+ Sbjct: 584 DISDRKLAEERIRELAFFDALTRLPNRTLLLDRLKQAIALSLRTKTCAALLFIDLDHFKT 643 Query: 572 INDKYGHEAGDELLVKFAERLIDAVREVDTVARLAGDEFTVILERLH-----GPADAMGV 626 +ND GHE GD LL + A+RL+ VRE DTVAR+ GDEF V+LE L+ + Sbjct: 644 LNDTIGHEKGDTLLKQVAQRLLANVRENDTVARVGGDEFVVVLEGLNELQQEAATQTRAI 703 Query: 627 AEKILVSMRRPFILTAATVNVSTSIGVAIYEPGQHIDQDALVAQADKAMYAAKRAGKN 684 EKIL ++ + L + SIG ++ G+ D L+ QAD AMY AK G+N Sbjct: 704 GEKILTALGSTYQLDDVEYRTTASIGATVF-LGRAASVDELMKQADLAMYKAKETGRN 760 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 145 bits (366), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 115/350 (32%), Positives = 172/350 (49%), Gaps = 25/350 (7%) Query: 351 YLREVRSLNEGLEQRVEDRTRRLHETAIALQQSLDNQR--ENQIRLEESETELRAILQNA 408 Y+R + + ++Q VE+RT L T L + +R E +RL E A NA Sbjct: 347 YMRALSMRSHRIQQLVEERTAELRTTNALLSDDIKARRLAERALRLRHQAIEASA---NA 403 Query: 409 HDAFIAIDEDGMVLEWNRQAEQLLGWAHKEAVGKELAELIIPFEQRELHRRGMIHFLNTG 468 A + D + N ++ G++ EAVGK + L E R+ G Sbjct: 404 ILILSAQEPDYTIEYVNPAFARITGYSSTEAVGKSIRFL----EGRDSDPEGF------- 452 Query: 469 EGMVINNR------IEINALRRDGNELPIEMTVGYVRRRAGHL--FIAFLHDITERQAFR 520 E + I R + + +DG+EL + + VR +G + F+A L+DIT + + Sbjct: 453 ETVWIAMREQREHNVVLRNYHKDGSELWNDFYIAPVRDESGEVTHFVAALYDITSMKRYE 512 Query: 521 NSLQEMALTDILTGLPNRRAFTEKLPEAMARAIREPQQMGLLFMDVDGFKSINDKYGHEA 580 L+ A D LTGL NR L +A+ A R+ Q + ++F+D+D FK +ND GH+A Sbjct: 513 AELEFQASRDTLTGLINRHMLRHHLEQAIGVAERQKQALWVVFVDLDRFKFVNDTLGHKA 572 Query: 581 GDELLVKFAERLIDAVREVDTVARLAGDEFTVILERLHGPADAMGVAEKILVSMRRPFIL 640 GD LL A RL AVRE DTVARL GDEF +IL ++I+ ++ P L Sbjct: 573 GDTLLRTVAGRLRAAVREADTVARLGGDEFIMILPEQDELTLDTRSLQRIMDAVAAPVTL 632 Query: 641 TAATVNVSTSIGVAIYEPGQHIDQDALVAQADKAMYAAKRAGKNRVEWNT 690 T+ ++ S+GVA Y P D + L+ AD AMY K+ G+N ++ T Sbjct: 633 GGHTLFMTCSVGVACY-PNDGTDGETLIKHADIAMYRGKQTGRNNFQFYT 681 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,141,305 Number of extensions: 91935 Number of successful extensions: 449 Number of sequences better than 1.0e-08: 40 Number of HSP's gapped: 387 Number of HSP's successfully gapped: 40 Length of query: 695 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 603 Effective length of database: 1,184,250 Effective search space: 714102750 Effective search space used: 714102750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)