BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0190.p (390 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313072|ref|YP_003777164.1| high-affinity K+ transport syst... 568 e-163 gi|300310937|ref|YP_003775029.1| heavy metal translocating P-typ... 83 4e-17 gi|300311691|ref|YP_003775783.1| cation transport ATPase [Herbas... 74 3e-14 gi|300312503|ref|YP_003776595.1| cation transport P-type ATPase ... 69 8e-13 gi|300311699|ref|YP_003775791.1| cation transport P-type ATPase ... 59 6e-10 >gi|300313072|ref|YP_003777164.1| high-affinity K+ transport system ATPase [Herbaspirillum seropedicae SmR1] Length = 730 Score = 568 bits (1465), Expect = e-163, Method: Compositional matrix adjust. Identities = 301/366 (82%), Positives = 329/366 (89%), Gaps = 9/366 (2%) Query: 21 SATATVPVVPVATSATKWMFDPAIVKPAVTDSFRKLSPRAQWRNPVMFVVYLGSILTTLL 80 SAT T +P A +WMFDP IVKPA+ DSFRKLSPRAQWRNPVMFVVYLGSILTTLL Sbjct: 11 SATVTPEAIPSAAQTARWMFDPKIVKPALADSFRKLSPRAQWRNPVMFVVYLGSILTTLL 70 Query: 81 YVQALNGHGEAPSGFILAVAVWLWFTVLFANFAEALAEGRSKAQAASLRSAKKSVVAKKL 140 ++QA G GEAP+GFI A+A WLWFTVLFANFAEALAEGRSKAQAA+LR KKSV+AKKL Sbjct: 71 FIQAAVGQGEAPAGFIFAIAAWLWFTVLFANFAEALAEGRSKAQAAALRGVKKSVMAKKL 130 Query: 141 QRVN--RND-------RCELVEGASLRKGDFVLIEAGDHVPADGEVVEGVATVDESAITG 191 +R + R D +CE+VEG SLRKGD VLIEAGD VPAD EV+EGVATVDESAITG Sbjct: 131 RRADHVRGDGMQVLASQCEVVEGDSLRKGDLVLIEAGDTVPADAEVIEGVATVDESAITG 190 Query: 192 ESAPVIRESGGDFTAVTGGTRVLSDWIIVRVTVNPGEAFLDRMIAMVESAKRQKTPNEIA 251 ESAPVIRESGGDF+AVTGGTRVLSDWI+VRV+VNPGEAFLDRMIAMVE A+RQKTPNE+A Sbjct: 191 ESAPVIRESGGDFSAVTGGTRVLSDWIVVRVSVNPGEAFLDRMIAMVEGARRQKTPNELA 250 Query: 252 LTLLLVALTIVFLLVTVTLLPFSVFSVNVAQSGAPVTVTALIALLVCLIPTTIGGLLSAI 311 LTLLLVALT+VFLLVTVTLLPFS+FSVN A SG+PV+VTALIALLVCLIPTTIGGLLSAI Sbjct: 251 LTLLLVALTLVFLLVTVTLLPFSIFSVNAAGSGSPVSVTALIALLVCLIPTTIGGLLSAI 310 Query: 312 GVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASAFLPANGVSEQELA 371 GVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQA+AF+PA GV+E+ELA Sbjct: 311 GVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQAAAFMPAPGVTERELA 370 Query: 372 DIAQLA 377 D+AQLA Sbjct: 371 DVAQLA 376 >gi|300310937|ref|YP_003775029.1| heavy metal translocating P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 82.8 bits (203), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 68/236 (28%), Positives = 116/236 (49%), Gaps = 8/236 (3%) Query: 118 EGRSKAQAASLRSAKKSVVAKKLQRVNRNDRCELVEGASLRKGDFVLIEAGDHVPADGEV 177 EGR+K Q A +A ++ + RV R+ + V A +R GD V++ G+ VP DG V Sbjct: 209 EGRAKRQTADAIAALNALRPEN-ARVRRDGQDIEVPLAQVRVGDLVVLRPGERVPVDGVV 267 Query: 178 VEGVATVDESAITGESAPVIRESGGDFTAVTGGTRVLSDWIIVRVTVNPGEAFLDRMIAM 237 EG + +DE+ +TGES P +++G TA G+ +++ T E L R++ M Sbjct: 268 REGRSHLDEAMLTGESRPQAKQAGDPLTA---GSINAEGVLVMETTAIGAETVLARIVRM 324 Query: 238 VESAKRQKTPNEIALTLLLVALTIVFLLVTVTLLPFSVFSVNVAQSGAPVTVTALIALLV 297 VE A+ K P + L+ ++ VF+ + L ++ + Q + +A++V Sbjct: 325 VEQAQAAKAP----IQRLVDQVSAVFVPAVLLLSLLTLLGWGLVQGNWEQALLNAVAVMV 380 Query: 298 CLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGTITLGN 353 P +G +AG + ++ A+E A V+ + DKTGT+TLG+ Sbjct: 381 IACPCALGLATPTAIMAGTGVAARHGILIKDAEALEIAHRVNAVAFDKTGTLTLGH 436 >gi|300311691|ref|YP_003775783.1| cation transport ATPase [Herbaspirillum seropedicae SmR1] Length = 853 Score = 73.6 bits (179), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 69/247 (27%), Positives = 114/247 (46%), Gaps = 24/247 (9%) Query: 119 GRSKAQAASLRSAKKSVVAKKLQRVNRNDRCELVEGASLRKGDFVLIEAGDHVPADGEVV 178 G+++ A+LR V+ RV R+ R + A L +GD +L+E G VPAD V+ Sbjct: 87 GKTERALAALR-----VLNSPTARVIRDGRTMRIAAAELVRGDLILLEEGARVPADAGVL 141 Query: 179 EGVA-TVDESAITGESAPVIR------------ESG--GDFTAVTGGTRVLSDWIIVRVT 223 E +DES +TGE+ PV + SG T V GT V+ I +VT Sbjct: 142 EASNLQLDESLLTGEACPVEKFAPSTKPEPHALPSGQTDSATLVRSGTLVVRGSGIAKVT 201 Query: 224 VNPGEAFLDRMIAMVESAKRQKTPNEIALTLLLVALTIVFLLVTVTLLPFSVFSVNVAQS 283 L ++ +++ + +P + + ++ L + L+++ L+ +S Sbjct: 202 ATGARTELGKISSLLHELVEEPSPMQRHIARIVTVLAVTGLMLSALLVVLEGYS----HG 257 Query: 284 GAPVTVTALIALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATSGRAVEAAGDVDVLLL 343 + IAL + L+P + +L+ G RM +ANV+ A+E G + VL Sbjct: 258 RWTEALLMGIALSMSLLPEELPIILTIFPAIGAWRMARANVLTRRLSAIETLGSISVLCT 317 Query: 344 DKTGTIT 350 DKTGT+T Sbjct: 318 DKTGTLT 324 >gi|300312503|ref|YP_003776595.1| cation transport P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 839 Score = 68.6 bits (166), Expect = 8e-13, Method: Compositional matrix adjust. Identities = 62/213 (29%), Positives = 96/213 (45%), Gaps = 17/213 (7%) Query: 151 LVEGASLRKGDFVLIEAGDHVPADGEVVEGVATVDESAITGESAPVIRESGGDFTAVTGG 210 LV A LR+GD +L GD + AD ++EG D + ++GES P + G + GG Sbjct: 336 LVVAAQLREGDVILARPGDTIAADACLLEGAGEFDLALLSGESRPQSFQIGQE---APGG 392 Query: 211 TRVLSDWIIVRVTVNPGEAFLDRMIAMVESAKRQKTP----NEIALTLLLVALTIVFLLV 266 LS + +RV GE+ L + + E A + K ++ + +VAL LL Sbjct: 393 AVNLSQPVFLRVVRATGESTLAALTRLAEQAGQGKPALAQWADVVASRFVVAL---LLLA 449 Query: 267 TVTLLPFSVFSVNVAQSGAPVTVTALIALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIA 326 T L + + A S A IA+LV P + + A R+++A + Sbjct: 450 GATFLAWQLIDPTRAWSTA-------IAVLVVSCPCALSLATPSALAAATDRLLRAGTLI 502 Query: 327 TSGRAVEAAGDVDVLLLDKTGTITLGNRQASAF 359 G +E D ++ DKTGT+T G Q +A Sbjct: 503 VRGNVLETLQRADTIVFDKTGTLTQGRPQLTAL 535 >gi|300311699|ref|YP_003775791.1| cation transport P-type ATPase [Herbaspirillum seropedicae SmR1] Length = 734 Score = 58.9 bits (141), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 76/286 (26%), Positives = 125/286 (43%), Gaps = 28/286 (9%) Query: 97 LAVAVWLWFTVLFANFAEALAEGRSKAQAASLRSAKK--SVVAKKLQRVNRNDRCELVE- 153 +A AV L ++ A A A GR+ A R+ ++ +++ K NR + +V Sbjct: 45 IAGAVLLREDLVAAVIALMFAGGRALESVAQRRAQQEMSALLGKAPHVANRYEGERIVTI 104 Query: 154 GASL-RKGDFVLIEAGDHVPADGEVVEGVATVDESAITGESAPVIRESGGDFTAVTGGTR 212 G +L R GD +L+ G+ VP DG ++ G A VDES +TGES PV G T +G Sbjct: 105 GLNLVRPGDRLLVRPGEPVPVDGRLL-GPAVVDESMLTGESLPVSHPQGA--TLASGCVN 161 Query: 213 VLSDWIIVRVTVNPGEAFLD--RMIAMVESAKRQ--KTPNEIALTLLLVALTIVFLLVTV 268 + + ++ + + F R++ ++++ + + AL + + L + L + Sbjct: 162 AGNAFDLLATSTDQSSTFAQVLRLVQAAQASQAPAARMADRYALLFIPLVLAMAALSWLL 221 Query: 269 TLLPFSVFSVNVAQSGAPVTVTALIALLVCLIPTTIGGLLSAIGVAGMSRMMQANVIATS 328 T P +V V + P L+ +P I V MSR Q V+ Sbjct: 222 TGDPRRALAVVVVATPCP---------LILAVPVAI--------VCAMSRCAQRGVLIKH 264 Query: 329 GRAVEAAGDVDVLLLDKTGTITLGNRQASAFLPANGVSEQELADIA 374 G A+E V L DKTGT+T G + + + EL +A Sbjct: 265 GGALEKLAQVRTLFFDKTGTLTAGKARLMNMVTTPSIQADELLRLA 310 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.132 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,074,980 Number of extensions: 40823 Number of successful extensions: 199 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 189 Number of HSP's successfully gapped: 6 Length of query: 390 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 303 Effective length of database: 1,207,925 Effective search space: 366001275 Effective search space used: 366001275 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)