BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0181.p (270 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309429|ref|YP_003773521.1| membrane protein [Herbaspirillu... 128 6e-31 >gi|300309429|ref|YP_003773521.1| membrane protein [Herbaspirillum seropedicae SmR1] Length = 262 Score = 128 bits (321), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 99/263 (37%), Positives = 139/263 (52%), Gaps = 19/263 (7%) Query: 26 LPVLHVLPAAAAESVGTAAPA--SSPAFTTTANIGLYSQYVFRGLTQTNEKPAVQGGLDL 83 + L + A AA V T A A + P + N + S Y +RGLTQT PA+Q G D Sbjct: 1 MKKLILATAIAASFVSTVARAEDAKPDNEFSFNAAVVSDYRYRGLTQTRFDPALQVGADY 60 Query: 84 SHE-SGLYAGIWASNISWISDTTPGAS------ASLEADLYGGFKYDFNDTLSGDVGFLH 136 +H +GLYAG W +NI WI D AS +E D+YGG + D + DVG L+ Sbjct: 61 THNPTGLYAGTWLTNIKWIKDAGAVASPPVNTKGDIEWDIYGGKRGDIGGGFTYDVGGLY 120 Query: 137 YAYPGSF--PNGFTKADTNELYLGLDAKWITLRYSYAVGNTFGSADTRGSSYVDLALTHE 194 Y YPG+ +G A+T ELY + +YS ++ N FG+A+++ S Y DL E Sbjct: 121 YYYPGNTLADSGAKNANTFELYGQVGYGPGYFKYSQSLTNLFGAANSKYSQYFDLGANIE 180 Query: 195 LVAGINGVAHLGHQRYTGPNSSNLSYTDWKLGLNR---DFSGYVLAAYYTGTNAKDAGYT 251 G+ H+GHQ G N + SYTDWK+G+++ D +G L Y GT+ KD T Sbjct: 181 TYYGVILGLHVGHQNVRG-NPNQFSYTDWKIGVSKTFDDLAGITLGLAYVGTDLKDGVTT 239 Query: 252 VKG----KNLGRDQLVLSVSRTF 270 KN+G++ V S+S+TF Sbjct: 240 TPASDGLKNVGKNGFVFSLSKTF 262 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.133 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 824,334 Number of extensions: 35628 Number of successful extensions: 93 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 88 Number of HSP's successfully gapped: 2 Length of query: 270 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 186 Effective length of database: 1,222,130 Effective search space: 227316180 Effective search space used: 227316180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)