BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0177.p (352 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309715|ref|YP_003773807.1| nitrate/sulfonate/bicarbonate A... 55 8e-09 >gi|300309715|ref|YP_003773807.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 319 Score = 55.1 bits (131), Expect = 8e-09, Method: Compositional matrix adjust. Identities = 51/218 (23%), Positives = 92/218 (42%), Gaps = 17/218 (7%) Query: 35 GVSQAQAQAKPKFKLAYAKCAHCMSMALVPELARN--VDIEGINFTAGSDALTALVSKSV 92 G S A A +L K AH +++ + E+A + ++ F G D + +++ + Sbjct: 15 GFSAASHAADDVIRLGNLKFAHYGAVSYIKEIAPKCGIKVDEKVFAKGPDVMQGILAGEL 74 Query: 93 DIAQVTYLSFVSGLDKGFDLVAVSGQVNGGSEMLVGKDMALASDDWAGLKKLIADYKAQG 152 D+ + +SG G + V+G GG+ ++ GKD + S + D K + Sbjct: 75 DVGTTASEAAISGRANGAPIYVVAGFAKGGARLVAGKDSGIKS---------VKDLKGK- 124 Query: 153 KPFRIAASRGNAQDLHMRGELALNGINPSKDVQFVNIPNPSDHAAALQRGEVELICSVEP 212 ++ +RG ++ + EL NG++ +KDV V + +D AL ++ + EP Sbjct: 125 ---KVGVTRGGIHEVLLDAELGQNGLS-AKDVTIVYLAF-ADLNQALLGKNIDAMMQSEP 179 Query: 213 FASQIRMTGVGKHFAFPYKQAAGRLTNLIVTRSDVIKE 250 +SQ G G PY G +V E Sbjct: 180 QSSQAINKGFGVEVMKPYDTPIGEPYRTMVMTEKFYNE 217 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.130 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 996,519 Number of extensions: 39785 Number of successful extensions: 145 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 146 Number of HSP's successfully gapped: 1 Length of query: 352 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 266 Effective length of database: 1,212,660 Effective search space: 322567560 Effective search space used: 322567560 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)