BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0176.p (274 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate A... 192 2e-50 gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate A... 156 2e-39 gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporte... 130 2e-31 gi|300310817|ref|YP_003774909.1| nitrate/sulfonate/bicarbonate A... 125 5e-30 gi|300310541|ref|YP_003774633.1| nitrate transporter permease [H... 107 7e-25 >gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 273 Score = 192 bits (489), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 110/255 (43%), Positives = 163/255 (63%), Gaps = 8/255 (3%) Query: 20 VPIFLVLFWEAFSRSGAIPASLLPAPSRVFATWMDWIFGYSEAAQDS--------SGRWI 71 VPI ++ WE SR+G I +LP+P V W+++ ++ SG + Sbjct: 15 VPIIVIALWEGASRAGWINPQILPSPWAVLRKWVEYATPLKPYDPEAGSWLAWAFSGELV 74 Query: 72 FDAMASCMRVTAGYLIAAMSGIALGVAIGWWRWVEKAIEPTVQMLRPIPPVSWIPLAIIW 131 DA+ S RV AG+LI A + LG+ +G + + + TVQ++RPIPP+++IPLAI+W Sbjct: 75 MDAIGSLYRVVAGFLIGAGLALPLGLLMGSSQRIYGLMNLTVQVIRPIPPIAYIPLAILW 134 Query: 132 FGIANKPAIFLVFLGAFFPILMNTVHGVKTSDRNLLRAAAMAGANQSQLLRFVILPAAMP 191 FG+ N PA+FL+ LGAFFP+LMNT+ GV+ D LRAA GA+QS L V+LPAA+P Sbjct: 135 FGLGNPPALFLISLGAFFPVLMNTIAGVRQVDSIYLRAARNLGASQSTLFLRVMLPAAVP 194 Query: 192 SIFAGLRIGIGSAWMLTVTAEMVAVKSGLGYALWDSYYFLRYDLVIAAMISIGLLGYLAD 251 I +G+RIGIG+A+++ + AEM+AV +GLG+ + ++ + D +IA MI+IGLLG D Sbjct: 195 YILSGVRIGIGTAFIVVIVAEMIAVSNGLGFRIMEAREYFWSDKIIAGMITIGLLGLAID 254 Query: 252 LILKLIMNSVLRWQH 266 L + + N +LRW Sbjct: 255 LGMSRLNNYMLRWHR 269 >gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 278 Score = 156 bits (395), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 95/247 (38%), Positives = 140/247 (56%), Gaps = 9/247 (3%) Query: 20 VPIFLVLFWEAFSRSGAIPASLLPAPSRVFATWMDWIFGYSEAAQDSSGRWIFDAMASCM 79 VP+ +L EA +R IPA LLP PS + T W G + AS Sbjct: 31 VPLAFLLLVEAGARLELIPARLLPPPSELAHTL--WSLA-------QRGELLPHIAASLA 81 Query: 80 RVTAGYLIAAMSGIALGVAIGWWRWVEKAIEPTVQMLRPIPPVSWIPLAIIWFGIANKPA 139 RV AG++I + +ALG+ +G R E +EP+ Q LR IP ++WIPL ++W GI P Sbjct: 82 RVAAGFVIGSALAVALGLLVGISRRAEALLEPSFQALRAIPSLAWIPLLLLWLGIDETPK 141 Query: 140 IFLVFLGAFFPILMNTVHGVKTSDRNLLRAAAMAGANQSQLLRFVILPAAMPSIFAGLRI 199 + L+ +GAFFP+ +N V G++ D L+ +G N L+ ++LPA++P +FAGLR Sbjct: 142 VVLIAIGAFFPVYLNLVAGIRDIDHKLIEVGTTSGLNTQALIGHILLPASLPYLFAGLRG 201 Query: 200 GIGSAWMLTVTAEMVAVKSGLGYALWDSYYFLRYDLVIAAMISIGLLGYLADLILKLIMN 259 G+ AWM V AE++A G+GY L D R DLV+AA++ + LLG L+D +L+ + Sbjct: 202 GLSLAWMFLVAAELIAATKGVGYLLSDGRESSRPDLVLAAILILALLGKLSDGLLRAVEQ 261 Query: 260 SVLRWQH 266 L W+ Sbjct: 262 KSLGWRE 268 >gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 279 Score = 130 bits (326), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 83/241 (34%), Positives = 133/241 (55%), Gaps = 11/241 (4%) Query: 26 LFWEAFSRSGAIPASLLPAPSRVF-ATWMDWIFGYSEAAQDSSGRWIFDAMASCMRVTAG 84 ++WE +++G + + +LP P V A W +SG S R +G Sbjct: 45 VWWEVAAQAGWLSSRILPEPLAVLKAAW----------ELSASGELWRHLAVSSGRAASG 94 Query: 85 YLIAAMSGIALGVAIGWWRWVEKAIEPTVQMLRPIPPVSWIPLAIIWFGIANKPAIFLVF 144 + + A G+ LG+ G R E ++ T+QM+R IP ++ IPL I+WFGI +FLV Sbjct: 95 FAVGAGLGLILGLLSGSLRSAELLLDTTLQMVRNIPALALIPLVILWFGIDETAKLFLVS 154 Query: 145 LGAFFPILMNTVHGVKTSDRNLLRAAAMAGANQSQLLRFVILPAAMPSIFAGLRIGIGSA 204 +G FFP+ +NT HG+++ D+ L+ A G + L R VILP A+P+I GLR +G Sbjct: 155 VGVFFPVYLNTFHGIRSVDQGLIEMAKSYGLSGWPLYRDVILPGALPAILVGLRFALGLV 214 Query: 205 WMLTVTAEMVAVKSGLGYALWDSYYFLRYDLVIAAMISIGLLGYLADLILKLIMNSVLRW 264 W+L + AE ++ ++G+GY ++ FL+ D+V+ ++ LLG LAD + + LRW Sbjct: 215 WVLLIVAETISAQAGIGYMTMNAREFLQTDVVLVGILLYALLGKLADTAARWLERFWLRW 274 Query: 265 Q 265 Sbjct: 275 H 275 >gi|300310817|ref|YP_003774909.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 256 Score = 125 bits (313), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 68/197 (34%), Positives = 117/197 (59%) Query: 66 SSGRWIFDAMASCMRVTAGYLIAAMSGIALGVAIGWWRWVEKAIEPTVQMLRPIPPVSWI 125 S G +AS R+ AG+ + G+ LG+ +G + V + ++P ++ R IPP++++ Sbjct: 56 SDGTLQQSVLASSGRILAGWGAGVVIGVPLGIFMGRFETVRQLLDPYIEFFRFIPPIAFV 115 Query: 126 PLAIIWFGIANKPAIFLVFLGAFFPILMNTVHGVKTSDRNLLRAAAMAGANQSQLLRFVI 185 LA+IW G I L+F F + +NT+ GV+ + LRAAA GA Q ++L VI Sbjct: 116 TLAVIWLGPGELSKIALIFYTTVFIVTLNTIAGVQAVNPLRLRAAASLGAGQWKILSTVI 175 Query: 186 LPAAMPSIFAGLRIGIGSAWMLTVTAEMVAVKSGLGYALWDSYYFLRYDLVIAAMISIGL 245 LP+ +P + G RI +G++++ V+AE+V+ + GLG +W + F R + V +I++G+ Sbjct: 176 LPSTVPFMVTGARIAMGNSFLTVVSAEIVSAREGLGALIWTARNFSRTEWVFVGIIALGI 235 Query: 246 LGYLADLILKLIMNSVL 262 LGYL D +L++ +L Sbjct: 236 LGYLFDRVLRVATRKLL 252 >gi|300310541|ref|YP_003774633.1| nitrate transporter permease [Herbaspirillum seropedicae SmR1] Length = 307 Score = 107 bits (268), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 54/174 (31%), Positives = 104/174 (59%), Gaps = 2/174 (1%) Query: 59 YSEAAQDSSGRWIFDAMASCMRVTAGYLIAAMSGIALGVAIGWWRWVEKAIEPTVQMLRP 118 Y + D W + +AS RV G+ +AA+ GI LG AIG ++ + P + +LRP Sbjct: 100 YRNSPNDQGIGW--NVLASLQRVGTGFGLAALVGIPLGFAIGRVEFLARMFGPIISLLRP 157 Query: 119 IPPVSWIPLAIIWFGIANKPAIFLVFLGAFFPILMNTVHGVKTSDRNLLRAAAMAGANQS 178 + P++W+P+ ++ F AN AI+ +F+ + +P+++NT GV+ ++ + A + ++ Sbjct: 158 VSPLAWLPIGLLVFKSANPAAIWSIFICSIWPMIINTAVGVQRVPQDYMNVARVLNLSEW 217 Query: 179 QLLRFVILPAAMPSIFAGLRIGIGSAWMLTVTAEMVAVKSGLGYALWDSYYFLR 232 ++L ++ P+ +P + G+R+ IG+AW++ V AEM+ G+G+ +WD + L Sbjct: 218 KVLTKILFPSVLPYMLTGVRLSIGTAWLVIVAAEMLTGGVGIGFWVWDEWNNLN 271 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.138 0.447 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 905,558 Number of extensions: 38674 Number of successful extensions: 211 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 200 Number of HSP's successfully gapped: 17 Length of query: 274 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 190 Effective length of database: 1,222,130 Effective search space: 232204700 Effective search space used: 232204700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)