BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0171.p (443 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirill... 722 0.0 gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmemb... 111 1e-25 gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysi... 109 5e-25 gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter prote... 75 8e-15 >gi|300310418|ref|YP_003774510.1| hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 722 bits (1864), Expect = 0.0, Method: Compositional matrix adjust. Identities = 339/439 (77%), Positives = 395/439 (89%), Gaps = 3/439 (0%) Query: 1 LENILLIVAALFLVALNGFFVAAEFGLVKLRQTRIRAIVKTQGLRGRILATVHNQLDAYL 60 +ENILLIV A FLVALNGFFVAAEF LVKLRQTRIRAI KTQG+RGRILA VHNQLDAYL Sbjct: 1 MENILLIVVAFFLVALNGFFVAAEFSLVKLRQTRIRAIAKTQGMRGRILAVVHNQLDAYL 60 Query: 61 SACQLGITLASLGLGWIGEPAFASLLEPLFALLGVSSPELIHGVSFVFAFVIISFLHIVV 120 SACQLGITLASLGLGWIGEPAFA +LEPLF+L GV++ ELIHGVSFVFAF +ISFLHIV Sbjct: 61 SACQLGITLASLGLGWIGEPAFARILEPLFSLAGVTNQELIHGVSFVFAFFVISFLHIVA 120 Query: 121 GELAPKSMAIRNPERIGLWCAMPLYGFYWTMYPAIWLLNLSANWVLRITGLGGVHGHDSH 180 GELAPKSMAIR+PE++GLWCAMPLYGFYW MYP IW+LN S+NW+LR+ GLG HGHD+H Sbjct: 121 GELAPKSMAIRSPEKLGLWCAMPLYGFYWGMYPLIWVLNASSNWLLRVAGLGAGHGHDAH 180 Query: 181 YSSDELKLILRSG---GKTGKFTPQEWSVLAQSLDFGDLKVMDLMRPANEIVALSAANTH 237 YSSDELKLILR+G GK GKFT EW+VL QSL+F +L V D+MRPA+EIVAL + Sbjct: 181 YSSDELKLILRAGNKSGKNGKFTRDEWNVLTQSLNFAELDVADIMRPASEIVALGDDKSL 240 Query: 238 DENMQIIYRNRFSRYPYFDAEQQEVLGLLHLKDVFIAQQDGKAIVDLRQYLRPVQYISPS 297 +EN+ IIYRNR+SRYPY+DAE+Q+VLGL+HLKDVF+AQQDG+AI +L+ YLRPVQYISP+ Sbjct: 241 EENLDIIYRNRYSRYPYYDAERQQVLGLVHLKDVFLAQQDGRAIANLKDYLRPVQYISPA 300 Query: 298 LSAQELLRRFRTGSPHFAIIGRKGQVPLGFVTLDNLLSVLVGEIRDEFRHTSNEWTRQDD 357 L A +LLRRFRTGSPHFA+IG+KGQ P GF+TLDN+LS+LVGEIRDEFRH + EWTRQDD Sbjct: 301 LPALDLLRRFRTGSPHFAVIGKKGQPPAGFITLDNMLSLLVGEIRDEFRHNTGEWTRQDD 360 Query: 358 GTLLGKGSLPIVTLEHMLGIDLEYDETVDSVGGLVMEKLGEVPKEGQKVEFHAFDIVVKK 417 GTLLGKGSLPIVTLE+MLGID+EYD+++DSVGGLVMEKLG++PKEGQK++F AFD+V+K+ Sbjct: 361 GTLLGKGSLPIVTLENMLGIDIEYDDSIDSVGGLVMEKLGDLPKEGQKIDFPAFDVVIKR 420 Query: 418 MKGPKILLVKVYPKMQDGE 436 M GPKI+LVKVYPK+Q+ Sbjct: 421 MSGPKIVLVKVYPKLQEAR 439 >gi|300309700|ref|YP_003773792.1| Mg2+/Co2+ transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 434 Score = 111 bits (278), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 108/430 (25%), Positives = 193/430 (44%), Gaps = 28/430 (6%) Query: 7 IVAALFLVALNGFFVAAEFGLVKLRQTRIRAIVKTQGLRGRILATVHNQLDAYLSACQLG 66 ++A L+ L+ FF E L+ + R+R + K QG R I T+ LD Sbjct: 10 VLALFILILLSAFFSMTETALMAANRHRLRHLAK-QGNRAAI--TILWLLDR-------- 58 Query: 67 ITLASLGLGWIGEPAFASLLEPLFALLGVSSPELIHGVSFVFAFVIISFLHIVVGELAPK 126 T L L I A +L L L +++ H A ++ L I+ E+ PK Sbjct: 59 -TERLLSLVLIANTAINALATALVTALAIAAFG-NHQRVITAATACVAALLIIFAEIVPK 116 Query: 127 SMAIRNPERIGLWCAMPLYGFYWTMYPAIWLLNLSANWVLRITGL-GGVHGHDSHYSSDE 185 + PERI L+ L P IW +NL + +LR+ + G + S +E Sbjct: 117 VIGATYPERISLFTGFILKPLMRVAKPLIWFVNLFVSAILRLLRIKTGARAAEQRISPEE 176 Query: 186 LKLILRSGGKTGKFTPQEW-SVLAQSLDFGDLKVMDLMRPANEIVALSAANTHDENMQII 244 L+ I+ G G F PQ+ S+L D + V D+M P +++ AL+ A D+ + + Sbjct: 177 LRSIVLEG---GSFIPQKHKSILLNLFDLEKISVEDVMTPRSQVEALNLAAPVDDILHQL 233 Query: 245 YRNRFSRYPYFDAEQQEVLGLLHLKDV--FIAQQDGKAIVDLRQYLRPVQYISPSLSAQE 302 ++ P F+ E ++G+LH++ +++ + D+R+ L Y+ Sbjct: 234 ATCYHNKLPVFEGEINHIVGILHVRKTVSLFRREEEISHDDIRELLSAPYYVPVETDVFT 293 Query: 303 LLRRFRTGSPHF-AIIGRKGQVPLGFVTLDNLLSVLVGEIRDEFRHTSNE---WTRQDDG 358 L+ F+ ++ G+V G VTL++++ ++GE +S E W + DG Sbjct: 294 QLQYFQENHERLGVVVDEYGEVQ-GLVTLEDIIEEMIGEFTTSSPGSSGEGFGWDK--DG 350 Query: 359 TLLGKGSLPIVTLEHMLGIDLEYDETVDSVGGLVMEKLGEVPKEGQKVEFHAFDIVVKKM 418 L +GS + + LG+ L D ++ GL++E L ++P+ V+ I + ++ Sbjct: 351 ACLLEGSTTLRDINKRLGLQLPLDGP-KTINGLLLEYLQDIPEASVAVKMDGCVIEIVQV 409 Query: 419 KGPKILLVKV 428 + I +VK+ Sbjct: 410 QNQAIRMVKL 419 >gi|300311018|ref|YP_003775110.1| CBS domains containing hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 442 Score = 109 bits (272), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 91/411 (22%), Positives = 176/411 (42%), Gaps = 11/411 (2%) Query: 17 NGFFVAAEFGLVKLRQTRIRAIVKTQGLRGRILATVHNQLDAYLSACQLGITLASLGLGW 76 + F AE + R+ R+ + K + ++ Q + + Q+G+ ++ G Sbjct: 17 SAFLSCAEISIAASRKIRLEMMSKEGVHNADRVLSLQAQPGNFFTVVQIGLNAVAILGGV 76 Query: 77 IGEPAFASLLEPLFALLGVSSPELIHGVSFVFAFVIISFLHIVVGELAPKSMAIRNPERI 136 +GEPA + + + + + SF +F++++ I+ +L PK +A+ PER Sbjct: 77 VGEPALTPHMREIISSFYIGP--WLENASFAMSFLVVTAFFILFADLMPKRLAMVAPERA 134 Query: 137 GLWCAMPLYGFYWTMYPAIWLLNLSANWVLRITGLGGVHGHDSHYSSDELKLILRSGGKT 196 + P+ P +W N + W+ + GL V + +E+ ++ +G + Sbjct: 135 AVALVKPMLLLETLFKPLVWFFNSLSKWIFTLFGLPQV--RQEVITPNEIHAMVDAGAQA 192 Query: 197 GKFTPQEWSVLAQSLDFGDLKVMDLMRPANEIVALSAANTHDENMQIIYRNRFSRYPYFD 256 G QE ++ + V M P + IV ++ Q I + SR+ D Sbjct: 193 GVLLTQEHHLIENVFELESRYVPSAMTPRDSIVYFLLTDSEATIRQKIIDHPHSRFLVCD 252 Query: 257 AEQQEVLGLLHLKDVFIAQQDGK--AIVDLRQYLRPVQYISPSLSAQELLRRFRTGSPHF 314 VLG + KDV G+ ++ D + + P I SL+ E+L R + Sbjct: 253 GHIDAVLGYIDSKDVLKRLLKGEQFSLAD-KGLVHPPLSIPDSLNLWEVLERMKAMDEDL 311 Query: 315 AIIGRKGQVPLGFVTLDNLLSVLVGEIRDEFRHTSNEWTRQDDGTLLGKGSLPIVTLEHM 374 AI+ + + +G ++ ++ VL+G + + R+D+ + L G P+ + Sbjct: 312 AIVVNEYALVVGIISTTDVTGVLMGNLL-AITEEEQQIVRRDENSWLMDGLTPLNDVMSE 370 Query: 375 LGIDLEYDETV--DSVGGLVMEKLGEVPKEGQKVEFHAFDIVVKKMKGPKI 423 L ID EY V +++ G +M L ++PK+ V F F V ++G +I Sbjct: 371 LDID-EYPNPVGYETLSGFIMYMLRKIPKKTDFVLFAEFKFEVVDIEGNRI 420 >gi|300310054|ref|YP_003774146.1| Mg2+ and Co2+ transporter protein [Herbaspirillum seropedicae SmR1] Length = 292 Score = 75.5 bits (184), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 64/260 (24%), Positives = 117/260 (45%), Gaps = 23/260 (8%) Query: 185 ELKLILRSGGKTGKFTPQEWSVLAQSLDFGDLKVMDLMRPANEIVALSAANTHDENMQII 244 EL +L + S++ DL V D+M P +++ + + + M + Sbjct: 35 ELLEVLHDAHERNLIDADALSMIEGVFQVSDLAVRDIMVPRSQMDTIDISKPIETWMPEV 94 Query: 245 YRNRFSRYPYFDAEQQEVLGLLHLKDV--FIAQQDGKAIVDLRQYLRPVQYISPSLSAQE 302 SR+P D E+ +V+G+L KD+ + A++ D+R LRP +I S Sbjct: 95 LATSHSRFPAVDGERDKVVGILLAKDLLRYYAEES----FDVRDMLRPAIFIPESKRLNV 150 Query: 303 LLRRFRTGSPHFAIIGRKGQVPLGFVTLDNLLSVLVGEIRDEFRHTSNEWTRQDDGTLLG 362 LLR FR H A++ + G +T++++L +VG+I DE+ ++ +D L Sbjct: 151 LLRDFRANRNHMAVVVDEYGGVAGLITIEDVLEQIVGDIEDEY-----DFDEAEDNVLAL 205 Query: 363 K-----GSLPIVTLEHMLGIDLEY-----DETVDSVGGLVMEKLGEVPKEGQKVEFHA-- 410 K + L + + E D+ D++GGLV +G VP++G + + Sbjct: 206 KDGPHGARWRVKALTEIEQFNEEVGSRFSDDDADTIGGLVANHVGRVPRKGDEFDIDGLH 265 Query: 411 FDIVVKKMKGPKILLVKVYP 430 F+++ + +LLV P Sbjct: 266 FEVLRADARQVHVLLVSQLP 285 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.141 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,388,657 Number of extensions: 60174 Number of successful extensions: 164 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 155 Number of HSP's successfully gapped: 4 Length of query: 443 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 355 Effective length of database: 1,203,190 Effective search space: 427132450 Effective search space used: 427132450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)