BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0170.p (190 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogen... 344 3e-96 gi|300313731|ref|YP_003777823.1| sugar phosphate dehydrogenase o... 95 4e-21 gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herb... 94 8e-21 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 84 7e-18 gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 77 8e-16 >gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 344 bits (882), Expect = 3e-96, Method: Compositional matrix adjust. Identities = 162/189 (85%), Positives = 175/189 (92%) Query: 1 MNVVDEAQVDKGIADAVAAYGTLDILISNAGIQIIAPIVDSTLENWRKMLAIHLDGAYLT 60 MNV DE QVDKG+ADAV YG LDI+ISNAGIQII+P+ + +LENWRKMLAIHLDGA+LT Sbjct: 61 MNVTDETQVDKGVADAVNTYGGLDIMISNAGIQIISPVAELSLENWRKMLAIHLDGAFLT 120 Query: 61 TRAAMRAMIKAGKGGSIIYMGSVHSHEASPLKAPYVTAKHGLIGLAKVVAKEGAKDKIRS 120 TRAAM+AMIK G+GGSIIYMGSVHSH ASPLKAPYVTAKHGL+GLAK VAKEGAKDKIR+ Sbjct: 121 TRAAMKAMIKQGRGGSIIYMGSVHSHLASPLKAPYVTAKHGLLGLAKTVAKEGAKDKIRT 180 Query: 121 NVICPGFVRTPLVEKQIPEQARELNISEEDVIKKVMLKDTVDGEFTTTADVAQTAVFLAA 180 NVICPGFVRTPLVEKQIPEQA+E ISEEDVIKK+MLKDTVDGEFTTT DVAQTAVFLAA Sbjct: 181 NVICPGFVRTPLVEKQIPEQAKEFGISEEDVIKKIMLKDTVDGEFTTTQDVAQTAVFLAA 240 Query: 181 FPTNALTGH 189 FPTNALTG Sbjct: 241 FPTNALTGQ 249 >gi|300313731|ref|YP_003777823.1| sugar phosphate dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 708 Score = 94.7 bits (234), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 66/196 (33%), Positives = 105/196 (53%), Gaps = 12/196 (6%) Query: 2 NVVDEAQVDKGIADAVAAYGTLDILISNAGIQIIAPIVDSTLENWRKMLAIHLDGAYLTT 61 ++ EA V + +G +D+L+SNAGI +P+ D++LE W++ L++ G +L + Sbjct: 505 DITSEADVTTILNSVALRFGGIDLLVSNAGIASASPLQDTSLEVWQRNLSVLATGYFLVS 564 Query: 62 RAAMRAMIKAGKGGSIIYMGSVHSHEASPLKAPYVTAKHGLIGLAKVVAKEGAKDKIRSN 121 RAA +M+ G GGSI+Y+ S + AS + Y TAK I LA+ +A EGA IR N Sbjct: 565 RAAFSSMLTQGLGGSIVYVASKNGLVASAGASAYCTAKAAEIHLARCIALEGAPHGIRVN 624 Query: 122 VICP-GFVRTPLVE--KQIPEQARELNISEEDV----IKKVMLKDTVDGEFTTTADVAQT 174 V+ P +R + K E+A I +DV ++ MLK +V E D+A+ Sbjct: 625 VVNPDAVIRGSRIWDGKWKEERAASNKIEADDVEEFYRQRSMLKRSVLPE-----DIAEA 679 Query: 175 AVFLAAFPTNALTGHL 190 F A+ + TG++ Sbjct: 680 VYFFASEKSAKSTGNI 695 >gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 94.0 bits (232), Expect = 8e-21, Method: Compositional matrix adjust. Identities = 58/190 (30%), Positives = 102/190 (53%), Gaps = 4/190 (2%) Query: 2 NVVDEAQVDKGIADAVAAY-GTLDILISNAGIQ-IIAPIVDSTLENWRKMLAIHLDGAYL 59 +V E +VD AD + G LD+LI+NAGI +P+ + L +W + LA++L G +L Sbjct: 62 DVSSEREVDAMFADLAQRWSGRLDVLINNAGIAGPTSPVEEVALSDWDQTLAVNLTGPFL 121 Query: 60 TTRAAMRAMIKAGKGGSIIYMGSVHSHEASPLKAPYVTAKHGLIGLAKVVAKEGAKDKIR 119 TR A+ ++K GG+I+ + SV L+ PY +K+G+IGL + A E IR Sbjct: 122 CTRRAV-PLLKKNGGGAIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIELGPSHIR 180 Query: 120 SNVICPGFVRTPLVEKQIPEQARELNISEEDVIKKVMLKDTVDGEFTTTADVAQTAVFLA 179 N + PG V E+ + +A I E++ ++++ + ++ + T D+A +FL Sbjct: 181 VNAVLPGIVEGARQERIVAAKAAAYGIGHEEMRQRLLSRVSLR-KMVTAEDIANQVIFLC 239 Query: 180 AFPTNALTGH 189 + +++G Sbjct: 240 SPAGASISGQ 249 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 84.0 bits (206), Expect = 7e-18, Method: Compositional matrix adjust. Identities = 57/191 (29%), Positives = 96/191 (50%), Gaps = 20/191 (10%) Query: 8 QVDKGIADAVAA--------YGTLDILISNAGIQIIAPIVDSTLENWRKMLAIHLDGAYL 59 Q+D G +++AA YG D+L++NAG+ +D LENW + + +++ G L Sbjct: 63 QMDLGSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLL 122 Query: 60 TTRAAMRAMIKAGKGGSIIYMGSVHSHEASPLKAPYVTAKHGLIGLAKVVAKEGAKDKIR 119 + A R M+K + G I+ + S+ A + Y T+K +IGL + +A E A+ I Sbjct: 123 AGQHAARLMVKQ-QWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGIT 181 Query: 120 SNVICPGFVRTPLVEKQIPEQARELNISEEDVIKKVMLKDTVDGEFTTTADVAQTAVFLA 179 N + PG V TP+ + EQ R E + V ++ + TT ++A +LA Sbjct: 182 VNSVAPGPVDTPMTQAMHSEQTR------ESYYRLVPMR-----RYGTTDEIASAVCYLA 230 Query: 180 AFPTNALTGHL 190 A + +TGH+ Sbjct: 231 AEESAYITGHV 241 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 77.0 bits (188), Expect = 8e-16, Method: Compositional matrix adjust. Identities = 55/182 (30%), Positives = 90/182 (49%), Gaps = 3/182 (1%) Query: 10 DKGIADAVAAYGTLDILISNAGIQIIAPIVDSTLENWRKMLAIHLDGAYLTTRAAMRAMI 69 D I VA GT+D+L + AG I++ + W ++ + T RA + M+ Sbjct: 66 DAAIKALVAKIGTIDVLFNCAGYVAAGNILECDDKAWDFSFNLNAKAMFHTIRAVLPGML 125 Query: 70 KAGKGGSIIYMGSVHSH-EASPLKAPYVTAKHGLIGLAKVVAKEGAKDKIRSNVICPGFV 128 A K GSI+ + S S + + Y +K ++GL K VA + IR N ICPG + Sbjct: 126 -AKKAGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVAQGIRCNAICPGTI 184 Query: 129 RTPLVEKQIPEQARELNISEEDVIKKVMLKDTVDGEFTTTADVAQTAVFLAAFPTNALTG 188 +P + ++I QA+E SEE+V + + + G +VA A++LA+ +N TG Sbjct: 185 ESPSLNQRISTQAKETGKSEEEVRAAFVGRQPM-GRIGKAEEVAALALYLASDESNFTTG 243 Query: 189 HL 190 + Sbjct: 244 SI 245 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 552,787 Number of extensions: 21215 Number of successful extensions: 136 Number of sequences better than 1.0e-08: 25 Number of HSP's gapped: 135 Number of HSP's successfully gapped: 25 Length of query: 190 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 110 Effective length of database: 1,241,070 Effective search space: 136517700 Effective search space used: 136517700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)