BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0163.p (276 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310583|ref|YP_003774675.1| methyl-accepting chemotaxis I (... 285 3e-78 gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis pro... 78 1e-15 gi|300309873|ref|YP_003773965.1| methyl-accepting chemotaxis tra... 60 2e-10 gi|300309376|ref|YP_003773468.1| methyl-accepting chemotaxis pro... 60 2e-10 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 59 4e-10 >gi|300310583|ref|YP_003774675.1| methyl-accepting chemotaxis I (serine chemoreceptor) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 574 Score = 285 bits (728), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 141/217 (64%), Positives = 181/217 (83%) Query: 1 MISNIKIGARLALGFCLVLLCAAAILLNGLWRMTQMESSSAYIIDQKVASMTAAMSMRES 60 MI +KIG+RLA GFCL+LL A AILL GLWRM ++E++S Y+ID+KVA+MTAA++MRE+ Sbjct: 1 MIQRLKIGSRLAFGFCLILLLATAILLIGLWRMAELEAASEYVIDEKVAAMTAALNMREA 60 Query: 61 GSALALALRKVVTPTDAAEGQQENQRLAKILQAYAGYEGQLTKLTSTDKGKALLTASAAE 120 GS LALALRKVVTPTDAAEG+ E+ L+KILQAYAG+E Q+T+L+ +GK LLTA++ + Sbjct: 61 GSQLALALRKVVTPTDAAEGKAEDAHLSKILQAYAGFEKQITELSPAGRGKELLTAASGQ 120 Query: 121 RKVLFPIVEKIREMVGAGNYFDAAQLLKSDFLPSYDKWMASVAALAAYQQEDMSAAYVEF 180 K LFPIVE IR+ V + NYFDAAQLLKSDFLP++DKWMASV ALAA+QQEDM AA+ E Sbjct: 121 SKQLFPIVETIRQQVASNNYFDAAQLLKSDFLPAHDKWMASVGALAAFQQEDMKAAHAEA 180 Query: 181 QNSYHSGRIGMIVIGLVTLLLGAFFTWSITRTITAPL 217 ++SY +IGM+++G++TL LG FFT+ ITR+I PL Sbjct: 181 KDSYRKAQIGMLLMGVLTLGLGFFFTFVITRSIVGPL 217 >gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 583 Score = 77.8 bits (190), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 50/215 (23%), Positives = 108/215 (50%) Query: 3 SNIKIGARLALGFCLVLLCAAAILLNGLWRMTQMESSSAYIIDQKVASMTAAMSMRESGS 62 +N+ I RLA+ F +++L A I+ GL R+ + + +++ + + ++ Sbjct: 4 NNLSIATRLAIAFSILILVIAGIVALGLARLADINKALDLVVNDRYKKIAIINTISGKVD 63 Query: 63 ALALALRKVVTPTDAAEGQQENQRLAKILQAYAGYEGQLTKLTSTDKGKALLTASAAERK 122 +A++LR + TD A+ E + + + +LT+L + + KA L RK Sbjct: 64 DVAISLRNQLLMTDPAQIAAEQKTIESLSAENTKLYDELTRLVANPEAKAKLDKVTETRK 123 Query: 123 VLFPIVEKIREMVGAGNYFDAAQLLKSDFLPSYDKWMASVAALAAYQQEDMSAAYVEFQN 182 + ++I ++ +G+ A +L+ S+ +P + + + L+ QQ+ M + + + Sbjct: 124 SYAALRKQIDKLQSSGDKDAAIKLMLSEMVPLQKIYFSQLDELSDTQQKLMDQSVDQAEA 183 Query: 183 SYHSGRIGMIVIGLVTLLLGAFFTWSITRTITAPL 217 +Y + R+ M++ G++ AF W+I+R+IT PL Sbjct: 184 AYETTRMIMVIAGVLAATFAAFIAWTISRSITRPL 218 >gi|300309873|ref|YP_003773965.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 547 Score = 60.1 bits (144), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 48/188 (25%), Positives = 90/188 (47%), Gaps = 5/188 (2%) Query: 2 ISNIKIGARLALGFCLVLLCAAAILLNGLWRMTQMESSSAYIIDQKVASMTAAMSMRESG 61 + N+K+ RLALGF L+L+ +I GL+ M ++ + I D A + ++R + Sbjct: 1 MGNLKVVTRLALGFGLILVFLLSISALGLYGMARVNDALTDITDVNNAEIKLVTALRNAL 60 Query: 62 SALALALRKVVTPTDAAEGQQENQRLAKILQAYAGYEGQLTKL-----TSTDKGKALLTA 116 S ALA+R + + A+ Q E+Q LA+ + YA L K+ +T++ + LL Sbjct: 61 SQRALAIRNIALLDEQADMQVESQTLARQEKLYADAYAALDKMFTTEAATTERERQLLVQ 120 Query: 117 SAAERKVLFPIVEKIREMVGAGNYFDAAQLLKSDFLPSYDKWMASVAALAAYQQEDMSAA 176 + P++ K ++ +A ++L + P W+ S+ LA ++ + A Sbjct: 121 IKNDEAATLPLMAKAVQLGLENKAAEAGKMLITQVRPKQQAWIKSLTELAEFEDQLNDEA 180 Query: 177 YVEFQNSY 184 +N+Y Sbjct: 181 ATSARNTY 188 >gi|300309376|ref|YP_003773468.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 534 Score = 60.1 bits (144), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 57/216 (26%), Positives = 99/216 (45%) Query: 2 ISNIKIGARLALGFCLVLLCAAAILLNGLWRMTQMESSSAYIIDQKVASMTAAMSMRESG 61 +SN+ I RL LGF L+++ A+ +G+ R+ Q+ + +I K A + + Sbjct: 3 LSNVTIRVRLWLGFGLLMVLMLAMAASGIARLGQLNAQMKDVIHDKYPKTVVAGDIVDQL 62 Query: 62 SALALALRKVVTPTDAAEGQQENQRLAKILQAYAGYEGQLTKLTSTDKGKALLTASAAER 121 + +A ++R ++ + + E +R++ +A QL KL + +A LT + Sbjct: 63 NLIARSVRNILLLKREPQIKSEYERISGADRAIREDLAQLDKLLTDGDSRAALTTLVTAQ 122 Query: 122 KVLFPIVEKIREMVGAGNYFDAAQLLKSDFLPSYDKWMASVAALAAYQQEDMSAAYVEFQ 181 F +K+ MV G A +L + P M + AL Q E M A E + Sbjct: 123 NAYFEKRDKVLAMVQQGGKEGAIDMLIDEVRPVQSATMNAADALIRQQHEMMKTAGDEVE 182 Query: 182 NSYHSGRIGMIVIGLVTLLLGAFFTWSITRTITAPL 217 ++Y R ++++ L A W ITR+ITAPL Sbjct: 183 SNYAQARTLLLLLAAAGLAAAAVIAWMITRSITAPL 218 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 58.9 bits (141), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 57/234 (24%), Positives = 105/234 (44%), Gaps = 36/234 (15%) Query: 2 ISNIKIGARLALGFCLVLLCAAAILLNGLWRMTQMESSSAYIIDQKVASMTAAM----SM 57 + N+KIG RLA+GF +L+ A ++ G+WR+ + ++ + + A+ ++ Sbjct: 1 MKNLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRIALKKERDAVQWHAAI 60 Query: 58 RESGSALALALRKVVTPTDAAEG---QQENQRLAKILQAYAGYEGQLTKLTSTDKGKALL 114 +E+G A+ K + DA + +Q + + A+I Q E +T Sbjct: 61 KENG-VRTFAVMK--SDDDAFQAYFQKQIDAQSARISQLQKEVEAAITD----------- 106 Query: 115 TASAAERKVLFPIVEKIR-----------EMVGAGNYFDAAQLLKSDFLPSYDKWMASVA 163 AE K LF V K+R ++ AG+ A ++ + + D + V Sbjct: 107 ----AEEKRLFDEVGKLRAAYRATRQSIFDVKTAGDDARAREMTDTTLVKMMDDYADGVL 162 Query: 164 ALAAYQQEDMSAAYVEFQNSYHSGRIGMIVIGLVTLLLGAFFTWSITRTITAPL 217 A +Q++ + Y SGR ++ +G + ++LGA W +TR+IT PL Sbjct: 163 RYADFQKQVIDQTAAAINRDYRSGRSMLLALGAIEIVLGAALAWLLTRSITRPL 216 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 802,772 Number of extensions: 31823 Number of successful extensions: 149 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 143 Number of HSP's successfully gapped: 14 Length of query: 276 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 192 Effective length of database: 1,222,130 Effective search space: 234648960 Effective search space used: 234648960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 130 (54.7 bits)