BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0145.p (162 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310592|ref|YP_003774684.1| phosphate regulon two-component... 289 6e-80 gi|300310718|ref|YP_003774810.1| phosphate regulon two-component... 189 1e-49 gi|300312680|ref|YP_003776772.1| two component response regulato... 117 3e-28 gi|300310849|ref|YP_003774941.1| two component transcription reg... 113 7e-27 gi|300313680|ref|YP_003777772.1| two component response regulato... 109 1e-25 >gi|300310592|ref|YP_003774684.1| phosphate regulon two-component response regulator transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 245 Score = 289 bits (740), Expect = 6e-80, Method: Compositional matrix adjust. Identities = 142/157 (90%), Positives = 148/157 (94%) Query: 6 APRAAXQLSVIMLTAKSMEEDKIAGLDNGADDYITKPFSPRELTARIKALLRRKSPEFAQ 65 A R QLSVIMLTAKSMEEDKIAGLDNGADDYITKPFSPRELTARIKALLRRKSPE AQ Sbjct: 89 ADRQLQQLSVIMLTAKSMEEDKIAGLDNGADDYITKPFSPRELTARIKALLRRKSPEHAQ 148 Query: 66 EEMTVGPVTLDPASCSVALSGDKIDIGHAEYKLLKFFMAHPDRVFSRSQLLDRVWGDHVV 125 E M+ GP+TLDPASCSV+LSG +IDIGHAEYKLLKFF+AHPDRVFSRSQLLDRVWGDHVV Sbjct: 149 EVMSAGPITLDPASCSVSLSGARIDIGHAEYKLLKFFLAHPDRVFSRSQLLDRVWGDHVV 208 Query: 126 IEERTVDVHVLRLRKALRDAEHLIKTVRSVGYMLSAK 162 IEERTVDVHVLRLRKALRDAE+LIKTVRSVGYMLS K Sbjct: 209 IEERTVDVHVLRLRKALRDAEYLIKTVRSVGYMLSTK 245 >gi|300310718|ref|YP_003774810.1| phosphate regulon two-component response regulator transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 230 Score = 189 bits (479), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 85/150 (56%), Positives = 117/150 (78%) Query: 13 LSVIMLTAKSMEEDKIAGLDNGADDYITKPFSPRELTARIKALLRRKSPEFAQEEMTVGP 72 L VIMLTAK MEEDK+ GL+ GADDY+TKPFSP+EL AR++ALLRRK+PE + + Sbjct: 80 LPVIMLTAKGMEEDKVKGLNEGADDYVTKPFSPKELVARVQALLRRKAPEIGSQALKYRD 139 Query: 73 VTLDPASCSVALSGDKIDIGHAEYKLLKFFMAHPDRVFSRSQLLDRVWGDHVVIEERTVD 132 +++D V+++ + + AE+KLL++ +AHP+R+FSR+QLLD+VWGDH IEERTVD Sbjct: 140 ISIDVERRQVSVADKPVLLDQAEFKLLRYLVAHPERIFSRAQLLDKVWGDHTFIEERTVD 199 Query: 133 VHVLRLRKALRDAEHLIKTVRSVGYMLSAK 162 VH++RLRK+L D H ++TVR +GY LSA+ Sbjct: 200 VHIMRLRKSLGDLAHYVQTVRGMGYKLSAE 229 >gi|300312680|ref|YP_003776772.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 220 Score = 117 bits (294), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 64/153 (41%), Positives = 97/153 (63%), Gaps = 6/153 (3%) Query: 9 AAXQLSVIMLTAKSMEEDKIAGLDNGADDYITKPFSPRELTARIKALLRRKSPEFAQEEM 68 A V++LTA+ D++ GL+ GADDY+ KPF+ EL ARIKAL+RR +P+ AQ + Sbjct: 67 AGNNTPVLLLTARDTIPDRVHGLELGADDYLVKPFATPELVARIKALIRRSAPQPAQ--L 124 Query: 69 TVGPVTLDPASCSVALSGDKIDIGHAEYKLLKFFMAHPDRVFSRSQLLDRV--WGDHVVI 126 TVG ++LD A+ + G KI++ E+ +L++ M H RV S+ Q++D V WG+ I Sbjct: 125 TVGGLSLDNATKRADIDGRKIELSLREWTVLEYLMQHASRVVSKQQIIDAVLPWGEDFTI 184 Query: 127 EERTVDVHVLRLRKALRDAEHLIKTVRSVGYML 159 V+V+V R+R + D+ I+T+R GYML Sbjct: 185 N--AVEVYVSRIRLKIADSGVAIRTIRGFGYML 215 >gi|300310849|ref|YP_003774941.1| two component transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 248 Score = 113 bits (283), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 71/164 (43%), Positives = 97/164 (59%), Gaps = 10/164 (6%) Query: 8 RAAXQLSVIMLTAKSMEEDKIAGLDNGADDYITKPFSPRELTARIKALLRRKS---PEFA 64 RA + VIMLTA+ D+I GL+ GADDY+ KPF PREL ARI+++LRR P A Sbjct: 74 RATSSVPVIMLTARGEPLDRILGLEMGADDYLAKPFEPRELFARIRSVLRRTQALPPNMA 133 Query: 65 QEE---MTVGPVTLD-PASCSVALSGDKIDIGHAEYKLLKFFMAHPDRVFSRSQLLDRVW 120 Q + M TLD A V G + + AE++LLK F+ HP+RV +R QLL+ Sbjct: 134 QPDVAAMRFAGWTLDLTARHLVNRDGVVVALSGAEFRLLKVFLDHPNRVLNRDQLLELTQ 193 Query: 121 GDHVVIEERTVDVHVLRLRKALRD---AEHLIKTVRSVGYMLSA 161 G +R+VD+ + RLR+ L D +IKTVR+ GY+L+ Sbjct: 194 GRESDPFDRSVDIQISRLRQKLGDDARTPTIIKTVRNEGYVLAT 237 >gi|300313680|ref|YP_003777772.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 218 Score = 109 bits (272), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 61/149 (40%), Positives = 93/149 (62%), Gaps = 3/149 (2%) Query: 13 LSVIMLTAKSMEEDKIAGLDNGADDYITKPFSPRELTARIKALLRRKSPEFAQEEMTVGP 72 + V++ TA+ D++AGLD GADDY+ KPF EL+AR++AL RR++ A+ + V Sbjct: 73 IPVLITTARDAVADRVAGLDAGADDYLIKPFDLEELSARMRALSRRQAGR-AESLVQVRE 131 Query: 73 VTLDPASCSVALSGDKIDIGHAEYKLLKFFMAHPDRVFSRSQLLDRVWGDHVVIEERTVD 132 V L+PA+ V +SG +++ E+ LL+ FM P V SR+QL ++++G IE V+ Sbjct: 132 VILNPATHEVTVSGKPVNLSAREFTLLQAFMDRPGVVLSRAQLEEKLYGWDDSIESNAVE 191 Query: 133 VHVLRLRKALRDAEHLIKTVRSVGYMLSA 161 VH+ LRK + IK VR VGY++ A Sbjct: 192 VHIHALRKKV--GSDFIKNVRGVGYLVPA 218 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 465,925 Number of extensions: 17113 Number of successful extensions: 136 Number of sequences better than 1.0e-08: 24 Number of HSP's gapped: 111 Number of HSP's successfully gapped: 24 Length of query: 162 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 84 Effective length of database: 1,250,540 Effective search space: 105045360 Effective search space used: 105045360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)