BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0144.p (439 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310593|ref|YP_003774685.1| phosphate regulon two-component... 706 0.0 gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 112 4e-26 gi|300313895|ref|YP_003777987.1| two-component system sensor his... 112 8e-26 gi|300309362|ref|YP_003773454.1| transmembrane sensory transduct... 107 2e-24 gi|300312677|ref|YP_003776769.1| two component hybrid sensor his... 99 7e-22 >gi|300310593|ref|YP_003774685.1| phosphate regulon two-component sensor kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 436 Score = 706 bits (1823), Expect = 0.0, Method: Compositional matrix adjust. Identities = 338/433 (78%), Positives = 377/433 (87%), Gaps = 1/433 (0%) Query: 1 MSPQLLFWVPAALRFLICLIGVCVVWFFFGATAGLIVALAGMGALAFTQLHYLFRLGSWL 60 MSPQLLFW+PA LR ++CL G VV FFFG AGL+ A+ G+GA+ T LHYL RL +WL Sbjct: 1 MSPQLLFWIPAVLRLVLCLAGAGVVGFFFGPIAGLVTAVLGVGAMMITHLHYLLRLSTWL 60 Query: 61 DNPSHAKLPDGWGAWTDIFARLYRMRRDDEKNQIELAEWLARFRQAMSLLPDGVVIMDDV 120 D+ ++LPDGWGAWTD++ARLYRMRRDDEKNQ ELAEWLARFRQAMSLLPDGVVIMDDV Sbjct: 61 DDSGQSRLPDGWGAWTDVYARLYRMRRDDEKNQAELAEWLARFRQAMSLLPDGVVIMDDV 120 Query: 121 LFLEWCNPAAQQHLGLQLERDKGMRVTNLIRNPAFIDYIILGRYEQPLTLSVQERKLIVQ 180 LFLEWCNPAAQQHLGL+L+RDKGMRVTNLIRNPAFIDYIILGRYEQPLT+++ ERKLI+Q Sbjct: 121 LFLEWCNPAAQQHLGLKLDRDKGMRVTNLIRNPAFIDYIILGRYEQPLTMTLHERKLIIQ 180 Query: 181 IIPFENRRQILVTHDATESERIDMMRRDFIANASHELRTPLTVINGFLEIALAQPNLDTD 240 IIPFENRRQILVTHD TESERIDMMRRDF+ANASHELRTPLTVINGFLEIALAQPNL+ Sbjct: 181 IIPFENRRQILVTHDVTESERIDMMRRDFVANASHELRTPLTVINGFLEIALAQPNLEEG 240 Query: 241 VRTAHLKLMTEQGQRMQNLIGDMLTLTRLESVDYPLRSERVHVRPLLEGILEEAKALSSG 300 R AHLKLMTEQGQRMQNLI DMLTLTRLES+DYPLR+E + VRPLLE I EE +ALS+G Sbjct: 241 TRMAHLKLMTEQGQRMQNLIDDMLTLTRLESIDYPLRTEVLRVRPLLERIAEEGRALSAG 300 Query: 301 KHQITLEADGPDLQGNVDELRSAFSNLVSNAVRYTPQDGIIKLYWERKAGGGAQFVVRDN 360 KH+I L DGPDL+GN +ELRSAF+NLV+NAVRYTP+ G I L W+ GA F V+D Sbjct: 301 KHEIVLTIDGPDLKGNAEELRSAFTNLVTNAVRYTPEGGRISLRWQSDE-QGAHFAVQDT 359 Query: 361 GIGISQEHISRLTERFYRVDKSRSRETQGTGLGLAIVRHVLLRHRATLGVESVPDKGSTF 420 GIGIS EHISRLTERFYRVDKSRSRETQGTGLGLAIVRHVLLRH ATL + SVPD GSTF Sbjct: 360 GIGISPEHISRLTERFYRVDKSRSRETQGTGLGLAIVRHVLLRHHATLEIASVPDHGSTF 419 Query: 421 TVGFPANVLIEEF 433 TV FP+ I+++ Sbjct: 420 TVHFPSTQTIQDY 432 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 112 bits (281), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 78/220 (35%), Positives = 120/220 (54%), Gaps = 20/220 (9%) Query: 196 ATESERIDMMRRDFIANASHELRTPLTVINGFLEIALAQPNLDTDVRTAHLKLMTEQGQR 255 ATE++R F+A+A+HELRTPLT + L+ LA+ +D + R A + +R Sbjct: 212 ATETQRA------FVADAAHELRTPLTALR--LQTQLAERAVDEEERRAAFADLKNGLER 263 Query: 256 MQNLIGDMLTLTRLESVDYPLRSERVHVRPLLEGILEEAKALSS-GKHQITLEADGPD-- 312 +L+ +LTL R E + +V +R LL ++ + +++ G + L G D Sbjct: 264 TTHLVHQLLTLARQEP--DAMSFTQVDLRALLGSVVGDLSSVAEHGGIDLGLSDQGDDGR 321 Query: 313 ---LQGNVDELRSAFSNLVSNAVRYTPQDGIIKLYWERKAGGGAQFVVRDNGIGISQEHI 369 + GN D LR F+NLV NA+RYTP G++ + + G VV+D+G GI++ + Sbjct: 322 PLRVTGNADALRILFNNLVDNALRYTPAGGVVDVGVDLSTAGYCTVVVQDSGPGIAESEL 381 Query: 370 SRLTERFYRVDKSR----SRETQGTGLGLAIVRHVLLRHR 405 R+ +RFYR S+ SR G+GLGLAIVR + HR Sbjct: 382 PRVFDRFYRAQHSQEGSGSRAAFGSGLGLAIVRQIADLHR 421 >gi|300313895|ref|YP_003777987.1| two-component system sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 112 bits (279), Expect = 8e-26, Method: Compositional matrix adjust. Identities = 78/236 (33%), Positives = 125/236 (52%), Gaps = 16/236 (6%) Query: 200 ERIDMMRRDFIANASHELRTPLTVINGFLEIALAQPNLDTDVRTAHLKLMTEQGQRMQNL 259 ER + +RR+F+A+ SHELRTPL ++NG LE AL L D +T L+ + + + + L Sbjct: 242 ERNEKLRREFMADVSHELRTPLAILNGQLE-ALEDGVLQPDAQT--LRSLKSEVEHLNKL 298 Query: 260 IGDMLTLTRLESVDYPLRSERVHVRPLLEGILEEAKALSSGKHQ-------ITLEADGPD 312 +GD+ L+ ++ R + + PLL ++ AL S + L A P Sbjct: 299 VGDLYDLSLADAGALTYRRADIDLAPLLR----DSAALYSDRFSQRGMRLSCELPASMP- 353 Query: 313 LQGNVDELRSAFSNLVSNAVRYTPQDGIIKLYWERKAGGGAQFVVRDNGIGISQEHISRL 372 + G+ L+ SNL N++RYT G ++++ + V D+ G+ E ++RL Sbjct: 354 VHGDAARLQQLLSNLFENSLRYTDAGGRLQVHAHLQQQHW-HLVFDDSEPGVPPEVMARL 412 Query: 373 TERFYRVDKSRSRETQGTGLGLAIVRHVLLRHRATLGVESVPDKGSTFTVGFPANV 428 ERF+RV+ SR+R + G GLGLAI R ++ H+ T+G + P G V PA + Sbjct: 413 FERFFRVEASRNRASGGAGLGLAICRRIVDAHQGTIGASASPLGGLRVVVVLPARL 468 >gi|300309362|ref|YP_003773454.1| transmembrane sensory transduction histidine kinase for cobalt/zinc/ cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 467 Score = 107 bits (268), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 87/248 (35%), Positives = 126/248 (50%), Gaps = 26/248 (10%) Query: 194 HDATES--ERIDMMRRDF------IANASHELRTPLTVINGFLEIALAQPNLDTDVRTAH 245 H+ ES E +D + R F A+ +H++RTPL + ++AL +P D A Sbjct: 225 HEIVESFNEMLDRLHRSFQQLSQFSADLAHDVRTPLNNLMVQTQVALGKPR-GIDEYQAL 283 Query: 246 LKLMTEQGQRMQNLIGDMLTLTRLESVDYPLR-------SERVHVRPLLEGILEEAKALS 298 L E+ +R+ ++ ML L R E+ L+ E + EGI +EA Sbjct: 284 LSSNIEEYERLSRMMESMLFLARAENAHVVLQWQELDVAQELTRIADYFEGIADEA---- 339 Query: 299 SGKHQITLEADGPD-LQGNVDELRSAFSNLVSNAVRYTPQDGIIKLYWERKAGGGAQFVV 357 Q+ L G LQ + LR A NLV+NA+RYTP DG+I+L + AG G + V Sbjct: 340 ----QVRLVVQGAGRLQADATLLRRAVGNLVANAIRYTPADGVIRLQVD-AAGDGMEISV 394 Query: 358 RDNGIGISQEHISRLTERFYRVDKSRSRETQGTGLGLAIVRHVLLRHRATLGVESVPDKG 417 + G GI E + RL +RFYR D SRS +GLGLAIVR +++ H V S P + Sbjct: 395 SNPGAGIPAEQLERLFDRFYRADPSRSSAGGSSGLGLAIVRSIMMLHGGQASVTSSPGQL 454 Query: 418 STFTVGFP 425 + F++ FP Sbjct: 455 TVFSLRFP 462 >gi|300312677|ref|YP_003776769.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1160 Score = 99.0 bits (245), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 81/249 (32%), Positives = 126/249 (50%), Gaps = 16/249 (6%) Query: 187 RRQILVTHDATESERIDMMRRDFIANASHELRTPL--TVINGFLEIALAQPNL-DTDVRT 243 + Q+++ ATE ER + + +F+AN SHELRTPL T+I L Q NL D VR Sbjct: 456 KTQVVLVEKATELERANQYKSEFLANMSHELRTPLNSTLILAKLLADNKQGNLSDEQVRF 515 Query: 244 AHLKLMTEQGQRMQNLIGDMLTLTRLESVDYPLRSERVHVRPLLE------GILEEAKAL 297 A + ++ G + LI D+L L+++E+ + E + L + I + K L Sbjct: 516 A--QTISSAGNDLLALINDILDLSKMEAGKLDIVPESFPLTKLADELSASFAITAQQKGL 573 Query: 298 SSGKHQITLEADGPD-LQGNVDELRSAFSNLVSNAVRYTPQDGIIKLYWERKAGGGAQFV 356 ++ +E P ++ ++ L NL+SNA+++T + G + L AGG F Sbjct: 574 G---FKVEVEPGVPQRMETDIQRLGQILKNLLSNAIKFT-EKGEVSLRIYPDAGGRIAFA 629 Query: 357 VRDNGIGISQEHISRLTERFYRVDKSRSRETQGTGLGLAIVRHVLLRHRATLGVESVPDK 416 VRD GIGI ++ + E F + D S R+ GTGLGL+I R + + V S + Sbjct: 630 VRDTGIGIGEDQHQFIFEAFRQADGSTHRKYGGTGLGLSISRDLAHLLGGDIRVTSRLGE 689 Query: 417 GSTFTVGFP 425 GS FT+ P Sbjct: 690 GSIFTLSLP 698 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.139 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,430,673 Number of extensions: 62694 Number of successful extensions: 315 Number of sequences better than 1.0e-08: 36 Number of HSP's gapped: 249 Number of HSP's successfully gapped: 36 Length of query: 439 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 351 Effective length of database: 1,203,190 Effective search space: 422319690 Effective search space used: 422319690 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)