BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0143.p (294 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogen... 102 5e-23 gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogen... 100 3e-22 gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenas... 95 6e-21 gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum s... 66 4e-12 >gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 288 Score = 102 bits (253), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 76/251 (30%), Positives = 129/251 (51%), Gaps = 5/251 (1%) Query: 1 MKNIGFIGVGMMGQGMAHNLVIKGFNVTVMGNRNRKPVEELVAE-GAREASSLQDLVANV 59 MK IG IG+G MG+GMA +L G+ V +G ++ +A+ G S+ L V Sbjct: 1 MKKIGVIGLGNMGRGMAFSLQRGGYEV--LGYDAQESTRTALAQQGLHCVDSIAALAPAV 58 Query: 60 NAIILCVTGSPQVEDLIFRAGGILENCRSGQILIDCSTSEPMSSARIAAQLATKGVSFVD 119 ++L + S VE ++ GG+L++ G +++D +T++P S+ R+AA+L G+ VD Sbjct: 59 EMVVLSLPTSDIVEQVVEGEGGLLQHGAPGLVIVDTTTADPNSTRRLAARLQAAGMELVD 118 Query: 120 APLARTPVEAMQGRLNTMVGADDATFAKVKPILEAFCENIFHIGGTTSAQKLKLLNNFIA 179 AP++ P A + ++G T A+++P+L H+G + KLLNN + Sbjct: 119 APVSGGPKGAATATMGMVIGGSAETVARIEPVLAVMSAKRVHVGPVGAGHVAKLLNNLVT 178 Query: 180 IGTATL-ATEALAMCDAVGVDQRKFFDVISKGGANSGIFQMIAGGILEGRSDGMKFSIAN 238 GT L A EA + A G+D R+ F ++ G ANS + ++ + + F++ Sbjct: 179 -GTHLLIAGEAARIAAAAGLDARQIFQGLAAGSANSKVIEVFYPTWIFNEAFDSGFTMKL 237 Query: 239 AGKDLSYYNNM 249 KDL N+ Sbjct: 238 MRKDLRLAMNL 248 >gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 297 Score = 99.8 bits (247), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 69/208 (33%), Positives = 110/208 (52%), Gaps = 3/208 (1%) Query: 3 NIGFIGVGMMGQGMAHNLVIKGFNVTVMGNRNRKPVEELVAEGAREASSLQDLVANVNAI 62 NI FIG+G MG MA NLV GF+V+ + + V++LV G + + VA + + Sbjct: 4 NIVFIGLGNMGLPMAQNLVKAGFSVSGH-DLVKASVDKLVEVGGKTEADSMAAVAKADVV 62 Query: 63 ILCVTGSPQVEDLIFRAGGILENCRSGQILIDCSTSEPMSSARIAAQLATKGVSFVDAPL 122 I + S VE L G+L + + G +LIDCST P ++ ++AA +G +DAP+ Sbjct: 63 ITMLPASRHVESLYLGEAGVLASAKPGTLLIDCSTIAPEAARKVAAAAKARGFEMLDAPV 122 Query: 123 ARTPVEAMQGRLNTMVGADDATFAKVKPILEAFCENIFHIGGTTSAQKLKLLNNFIAIGT 182 + A G L MVG F +P L+ + I+H G + S Q +K+ NN + +G Sbjct: 123 SGGTNGATAGTLTFMVGGSKEAFEAAQPYLQKMGKAIYHAGESGSGQTVKVCNNML-LGI 181 Query: 183 ATLAT-EALAMCDAVGVDQRKFFDVISK 209 + T EA+ + A G+D + +++SK Sbjct: 182 LMIGTSEAIRLGMANGMDPKVLSEIMSK 209 >gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 298 Score = 95.1 bits (235), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 60/227 (26%), Positives = 112/227 (49%), Gaps = 2/227 (0%) Query: 2 KNIGFIGVGMMGQGMAHNLVIKGFNVTVMGNRNRKPVEELVAEGAREASSLQDLVANVNA 61 +N+G IG+G MG G+A +L+ GFNV R ++ +++ A G ++ +L V+ Sbjct: 3 RNVGVIGLGAMGYGVASSLLRAGFNVHACDVR-KEVLDKFAAAGGVACANPAELAGKVDV 61 Query: 62 IILCVTGSPQVEDLIFRAGGILENCRSGQILIDCSTSEPMSSARIAAQLATKGVSFVDAP 121 +I V + Q E ++F G + + G ++I +T P + + +L KG+ +DAP Sbjct: 62 VITLVVNAAQTETVLFGDNGAVSAMKPGSVVISSATVAPDFAIELGKRLVEKGLLLLDAP 121 Query: 122 LARTPVEAMQGRLNTMVGADDATFAKVKPILEAFCENIFHIGGTTS-AQKLKLLNNFIAI 180 ++ A G + M +AK++ +L ++ +G T K+K++N +A Sbjct: 122 VSGGAARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLAG 181 Query: 181 GTATLATEALAMCDAVGVDQRKFFDVISKGGANSGIFQMIAGGILEG 227 + EA+A+ GV+ ++VI+ NS +F+ IL G Sbjct: 182 VHIAASAEAMALGLREGVNPDALYEVITHSAGNSWMFENRVPHILSG 228 >gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 292 Score = 65.9 bits (159), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 66/243 (27%), Positives = 116/243 (47%), Gaps = 5/243 (2%) Query: 3 NIGFIGVGMMGQGMAHNLVIKGFNVTVMGNRNRKPVEELVAEGAREASSLQDLVANVNAI 62 I F+G+G MG MA NLV G+ V NR+ PVE L A GA+ A++ + A+ + + Sbjct: 2 KIAFLGLGTMGFPMAANLVRAGYAVRAW-NRSPAPVERLAALGAQAAATPAEAAADAD-V 59 Query: 63 ILCVTGSPQVEDLIFRAGGILENCRSGQILIDCSTSEPMSSARIAAQLATKGVSFVDAP- 121 ++ + + G L R G I ++ +T + R+A +GV++V AP Sbjct: 60 LISMLADDDATETSLLDAGALAALRPGAIHVNMATISVALALRLAGLHQARGVAYVAAPV 119 Query: 122 LARTPVEAMQGRLNTMVGADDATFAKVKPILEAFCENIFHIG-GTTSAQKLKLLNNFIAI 180 L R V A G+LN + ++ A V+P+L+ + + +G A KL NF+ Sbjct: 120 LGRVNV-AEAGQLNILAAGEEQALAAVQPLLDVLGQKTWRLGRQPEQANAAKLAMNFMIA 178 Query: 181 GTATLATEALAMCDAVGVDQRKFFDVISKGGANSGIFQMIAGGILEGRSDGMKFSIANAG 240 +EA+A+ G+D+ F ++ + + +++ I + R + F +A Sbjct: 179 SAIGAMSEAVALVQGYGLDKAGFIELATSTAFAAPVYKGYGQAIADDRFEPAGFKLALGL 238 Query: 241 KDL 243 KD+ Sbjct: 239 KDV 241 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 848,306 Number of extensions: 33052 Number of successful extensions: 126 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 120 Number of HSP's successfully gapped: 4 Length of query: 294 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 210 Effective length of database: 1,222,130 Effective search space: 256647300 Effective search space used: 256647300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)