BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0130.p (146 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313809|ref|YP_003777901.1| hypothetical protein Hsero_4527... 102 9e-24 >gi|300313809|ref|YP_003777901.1| hypothetical protein Hsero_4527 [Herbaspirillum seropedicae SmR1] Length = 150 Score = 102 bits (255), Expect = 9e-24, Method: Compositional matrix adjust. Identities = 58/132 (43%), Positives = 82/132 (62%), Gaps = 2/132 (1%) Query: 5 KSLYERFLHAVLFEIVALLLCAPVMSWLLGVSFAHAGALTLMISLVAMTWNVIFNTVFDR 64 + + ++A LFE +A++ +S L G HA A + S +A WN IFN +F+R Sbjct: 2 QGFKRKLVYACLFEALAIVFTTVGLSVLGGHDSGHATAAAVASSTIAFVWNFIFNGLFER 61 Query: 65 LERRWKAPAAERSFAVRIGHAIVFEAGLILAVVPLAAWWLNIGLIEAFVLDIGLVLFFLP 124 E R P R+FA R HAI FE GL+L +VPL AWWL+I L AFVLD+GL++FF+ Sbjct: 62 WEAR--QPRRGRNFARRAAHAIGFEGGLVLMLVPLFAWWLDISLWNAFVLDLGLIVFFMI 119 Query: 125 YTLVFNWCYDKM 136 YT +FN +D++ Sbjct: 120 YTYLFNLLFDRI 131 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.334 0.142 0.467 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 479,347 Number of extensions: 19844 Number of successful extensions: 111 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 110 Number of HSP's successfully gapped: 1 Length of query: 146 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 69 Effective length of database: 1,255,275 Effective search space: 86613975 Effective search space used: 86613975 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.6 bits) S2: 126 (53.1 bits)