BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0125.p (439 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 419 e-118 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 411 e-116 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 360 e-100 gi|300309876|ref|YP_003773968.1| major facilitator superfamily p... 338 6e-94 gi|300313342|ref|YP_003777434.1| major facilitator superfamily p... 327 2e-90 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 419 bits (1077), Expect = e-118, Method: Compositional matrix adjust. Identities = 209/420 (49%), Positives = 281/420 (66%), Gaps = 1/420 (0%) Query: 15 ESNLYRRITLRFVPLLVICFVFAYLDRVNISFAKLQMQQDLGFSDAVYGLGASIFFVGYF 74 E Y ++T R +PLL +C+V +YLDRVN+ FAKLQM QDL FSD VYGLGA IFF+GYF Sbjct: 17 EDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYF 76 Query: 75 LFEIPSNMILHKVGPKRWIARIMVTWGIASAAMMFVTNETGFYILRFLIGSLEAGFVPGA 134 +FE+PSNMILHKVG + WIARIM+TWGI S AM +VT FYI+RFL+G EAGF PG Sbjct: 77 VFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGV 136 Query: 135 LYFFTNWFPSQRRGRINSLFMAAIALCGMIGGPISGGIMKFTHGVSSLHGWQWLFLLEGI 194 + + T W+P+ RRG+I +LFM IAL G+IGGP+SG IM GV GWQW+F+LE I Sbjct: 137 ILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAI 196 Query: 195 PSIVLGVICWIVIDNKIEDARWLKDDEKKLLLARLAAEPKSEAVHSFRDALKQPSTITMS 254 PS++LGV+ + + ++I DA WL ++EK+LL A ++ E + S + Sbjct: 197 PSLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHEESQKEHLSLGAMFANGRVWLGA 256 Query: 255 LIYLVLASGIYGLVFWMPQLIKTAGTDDTFIIGLITVIPYLCAAIGMILIGRSSDRTGER 314 LIY G+YG+ FW+P +IK G D +GL+T IPY AA M+LIGRS+D ER Sbjct: 257 LIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARRER 316 Query: 315 RWHLAGCALAGAVGYAVSAYFGSNTLVLVVGLSFAATGIIAGIGLFWILPPRVLTGAAAA 374 RWH+A A G +G +S +G NT + + L+ A+ GI+ + LFW LP L+G AAA Sbjct: 317 RWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGTAAA 376 Query: 375 GGIALINSVGQLGGIITPYMVGKIRDITGSASLGLYVVALECLVGAALLLWALPKRIYFR 434 GIALINS+G L G ++P++VG ++D T S + G+YV+A ++G L L LP ++ R Sbjct: 377 AGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVIGGLLTL-TLPAKLVNR 435 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 411 bits (1057), Expect = e-116, Method: Compositional matrix adjust. Identities = 208/419 (49%), Positives = 281/419 (67%), Gaps = 1/419 (0%) Query: 11 QDIQESNLYRRITLRFVPLLVICFVFAYLDRVNISFAKLQMQQDLGFSDAVYGLGASIFF 70 D + LY+++ R +P L++C+V AYLDRVN+ FAKLQM QDLGFS+ V+GLGA +FF Sbjct: 14 NDKSKDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFF 73 Query: 71 VGYFLFEIPSNMILHKVGPKRWIARIMVTWGIASAAMMFVTNETGFYILRFLIGSLEAGF 130 +GYFLFE+PSN+++HKVG + WIARIM+TWGI SAA MFV N T FYILRFL+G EAGF Sbjct: 74 IGYFLFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAGF 133 Query: 131 VPGALYFFTNWFPSQRRGRINSLFMAAIALCGMIGGPISGGIMKFTHGVSSLHGWQWLFL 190 PG + + T WFPS RR ++ ++FM+ I + G++G P+SG IM H L GWQW+FL Sbjct: 134 YPGIILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFL 193 Query: 191 LEGIPSIVLGVICWIVIDNKIEDARWLKDDEKKLLLARLAAEPK-SEAVHSFRDALKQPS 249 +E IP++++GV + +DN ++ A+WL D+EK L A + + K E+ H F +K Sbjct: 194 IEAIPAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESKHGFGAIVKDAR 253 Query: 250 TITMSLIYLVLASGIYGLVFWMPQLIKTAGTDDTFIIGLITVIPYLCAAIGMILIGRSSD 309 M LIY G YGL WMP L+K G IGL++ IP+ CA I M LIGRS+D Sbjct: 254 VWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRSAD 313 Query: 310 RTGERRWHLAGCALAGAVGYAVSAYFGSNTLVLVVGLSFAATGIIAGIGLFWILPPRVLT 369 R ERRWHL AL G VG+ +A F NT V + LS AA G++ LFW LP L+ Sbjct: 314 RMRERRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTAFLS 373 Query: 370 GAAAAGGIALINSVGQLGGIITPYMVGKIRDITGSASLGLYVVALECLVGAALLLWALP 428 GAAAA GIA INSVG L G ++PY++G ++D+T + + G+Y++A+ +VG+ L P Sbjct: 374 GAAAAVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLAVMLVVGSIATLATKP 432 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 360 bits (925), Expect = e-100, Method: Compositional matrix adjust. Identities = 196/409 (47%), Positives = 260/409 (63%), Gaps = 5/409 (1%) Query: 19 YRRITLRFVPLLVICFVFAYLDRVNISFAKLQMQQDLGFSDAVYGLGASIFFVGYFLFEI 78 YR+I R +P LV FV A++DRVN+ FAKLQM QDL FS+AVYGLGA IFF+GYF+FE+ Sbjct: 25 YRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFIFEV 84 Query: 79 PSNMILHKVGPKRWIARIMVTWGIASAAMMFVTNETGFYILRFLIGSLEAGFVPGALYFF 138 PSN++L K+G ++ +ARI + WG+AS AM +VT T FYILRFL+G EAGF PG + + Sbjct: 85 PSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGVVLYL 144 Query: 139 TNWFPSQRRGRINSLFMAAIALCGMIGGPISGGIMKFTHGVSSLHGWQWLFLLEGIPSIV 198 T WFP+QRR R+N LFM + A+ G +GGPI+G IM GV L WQWLF+LEGIPSI+ Sbjct: 145 TYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGIPSIL 204 Query: 199 LG--VICWIVIDNKIEDARWLKDDEKKLLLARLAAEPKSEAVH-SFRDALKQPSTITMSL 255 G V+ W + + +A+WL + EK + LAAE + H SF+DA + Sbjct: 205 AGFAVLAW--LPERPANAKWLSEAEKNAVTQVLAAENSAPGKHFSFKDACTNYRVWICAA 262 Query: 256 IYLVLASGIYGLVFWMPQLIKTAGTDDTFIIGLITVIPYLCAAIGMILIGRSSDRTGERR 315 +Y + SG + FW P +IK G IGLI+ IP++ + MI G SD+TGERR Sbjct: 263 VYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHSDKTGERR 322 Query: 316 WHLAGCALAGAVGYAVSAYFGSNTLVLVVGLSFAATGIIAGIGLFWILPPRVLTGAAAAG 375 H A L +G + N + + L+ AA GI+A +FW +P L G AAAG Sbjct: 323 LHSAIATLIACIGLVATGMTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFLAGTAAAG 382 Query: 376 GIALINSVGQLGGIITPYMVGKIRDITGSASLGLYVVALECLVGAALLL 424 GIALINS+G L G + PYM+G ++ TGS S GLY VA LV + L++ Sbjct: 383 GIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALELVASFLVV 431 >gi|300309876|ref|YP_003773968.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 449 Score = 338 bits (866), Expect = 6e-94, Method: Compositional matrix adjust. Identities = 175/396 (44%), Positives = 248/396 (62%), Gaps = 6/396 (1%) Query: 15 ESNLYRRITLRFVPLLVICFVFAYLDRVNISFAKLQMQQDLGFSDAVYGLGASIFFVGYF 74 E +YR++ LR +P L IC+V ++LDR+NI FA+LQM+QDLGFSDA+YGLGA+IF+VGY Sbjct: 21 EDAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKQDLGFSDAMYGLGAAIFYVGYV 80 Query: 75 LFEIPSNMILHKVGPKRWIARIMVTWGIASAAMMFVTNETGFYILRFLIGSLEAGFVPGA 134 LFE+PSNM L + G +R +RIMV WG+AS MM VT+ FY LRFL+G EAGF PG Sbjct: 81 LFEVPSNMALARFGARRTFSRIMVLWGLASVGMMAVTDAAHFYTLRFLLGVFEAGFFPGI 140 Query: 135 LYFFTNWFPSQRRGRINSLFMAAIALCGMIGGPISGGIMKFTHGVSSLHGWQWLFLLEGI 194 + + + WFP++RR + ++F A +A+ G++GG +SG IM+ GV L+GWQW+F +EG Sbjct: 141 VLYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWIMRDMAGVMGLYGWQWMFAIEGA 200 Query: 195 PSIVLGVICWIVIDNKIEDARWLKDDEKKLLLARLAAEP------KSEAVHSFRDALKQP 248 P++VLG++ ++ + A+WL +E+ L AR E ++ + R AL Sbjct: 201 PAVVLGLLAIFLLADGPAQAKWLSPEERSFLQARHQQEALRTHHVSGHSLAALRQALSHR 260 Query: 249 STITMSLIYLVLASGIYGLVFWMPQLIKTAGTDDTFIIGLITVIPYLCAAIGMILIGRSS 308 +T + IY L G L FWMP +I+ G D + L +VIP A+G+ILI R S Sbjct: 261 TTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVEVSLYSVIPNAIGAVGLILIARRS 320 Query: 309 DRTGERRWHLAGCALAGAVGYAVSAYFGSNTLVLVVGLSFAATGIIAGIGLFWILPPRVL 368 DR ER H A C GA+ A S+ V + LS AA I A + +FW LPP +L Sbjct: 321 DRRNERHGHFAVCTTGGALALAALTLHPSSFAVSLGLLSVAAVLIFAALPIFWSLPPGLL 380 Query: 369 TGAAAAGGIALINSVGQLGGIITPYMVGKIRDITGS 404 A GIA I+SVG GI++P+++G ++ TG+ Sbjct: 381 PAQTIAPGIAFISSVGITSGIVSPWLIGLVKTRTGN 416 >gi|300313342|ref|YP_003777434.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 327 bits (837), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 175/426 (41%), Positives = 256/426 (60%), Gaps = 15/426 (3%) Query: 8 ATAQDIQESNLYRRITLRFVPLLVICFVFAYLDRVNISFAKLQMQQDLGFSDAVYGLGAS 67 + +Q LY+++T + +P L++ +V AYLDRVN+ FAKL M DLG S+A YGLGA Sbjct: 5 SQGSAVQFDGLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYGLGAG 64 Query: 68 IFFVGYFLFEIPSNMILHKVGPKRWIARIMVTWGIASAAMMF---------VTNETGFYI 118 IFF+GYF+FE+PSN+ILH+VG K WIARIM+TWG+ S+ M F V+NET FY Sbjct: 65 IFFLGYFIFEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNETVFYT 124 Query: 119 LRFLIGSLEAGFVPGALYFFTNWFPSQRRGRINSLFMAAIALCGMIGGPISGGIMKFTHG 178 LRFL+G+ EAGF PG + + T W+P+ R+ ++ + ++GGPISG +M+F Sbjct: 125 LRFLLGASEAGFFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALMQFMGS 184 Query: 179 VSSLHGWQWLFLLEGIPSIVLGVICWIVIDNKIEDARWLKDDEKKLLLARLAAEPKSEAV 238 SL GWQW+F++E +P+ +LG++ V+ N I A+WL +EK L RLAAE K + Sbjct: 185 GDSLAGWQWMFVIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAENKEKVN 244 Query: 239 HSFRDALKQPSTITMSLIYLVLASGIYGLVFWMPQLIKTAGTDDTFIIGLITVIPYLCAA 298 + P ++L+YL++ G YG+ FW P +I+ AG IG+I+ IPY+ A Sbjct: 245 VPLKSLYALPILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGMISAIPYILGA 304 Query: 299 IGMILIGRSSDRTGERRWHLAGCALAGAVGYAVSAYFGSNTLVLVVGLSFAATGIIAGIG 358 M+ I R +++ G+ R + ++ + + F N + + G++ A G + I Sbjct: 305 TAMVFITRHAEKAGKHRSYSVAVSMVAGLALVLCTLFPHNIYITLAGIAIAVGGSLTSIA 364 Query: 359 LFWILPPRVLTGAAAAGGIALINSVGQLGGIITPYMVGKIRDITGSASLGLYVVALECLV 418 LFW P TGA AA GIA+INSVG LGG + PY++G + GS GL ++ Sbjct: 365 LFWGFPGAYFTGATAAAGIAIINSVGNLGGFVGPYLLGALTTAFGSPRYGL------MML 418 Query: 419 GAALLL 424 G AL L Sbjct: 419 GGALFL 424 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.143 0.448 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,282,788 Number of extensions: 51049 Number of successful extensions: 353 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 313 Number of HSP's successfully gapped: 22 Length of query: 439 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 351 Effective length of database: 1,203,190 Effective search space: 422319690 Effective search space used: 422319690 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)