BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0124.p (250 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310595|ref|YP_003774687.1| 3-oxoacyl-(acyl-carrier protein... 471 e-134 gi|300309919|ref|YP_003774011.1| 3-oxoacyl-(acyl-carrier protein... 194 4e-51 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 131 6e-32 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 127 1e-30 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 115 3e-27 >gi|300310595|ref|YP_003774687.1| 3-oxoacyl-(acyl-carrier protein) reductase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 471 bits (1211), Expect = e-134, Method: Compositional matrix adjust. Identities = 228/250 (91%), Positives = 240/250 (96%) Query: 1 MNHYDFQGRSAIITGGAQGIGFAIAERLLQGKAKVVLWDLDEKLLQEARAKLESLGNVET 60 MNHYDFQGRSAIITGGAQG G+A+AERLLQGKAKVVLWD+DEK L EAR KLESLGNVET Sbjct: 1 MNHYDFQGRSAIITGGAQGFGYAVAERLLQGKAKVVLWDMDEKALAEARRKLESLGNVET 60 Query: 61 VAVNIADQAAVQRAIEATEKVTQSIDVLVHSAGIAGQNGLVADYSPEEWRRVIDIDLNGA 120 V V+I+ QA VQ+AIEATEK+TQSID+LVHSAGIAGQN VA+YSPEEWRRVIDIDLNGA Sbjct: 61 VKVDISSQAEVQQAIEATEKLTQSIDILVHSAGIAGQNNPVAEYSPEEWRRVIDIDLNGA 120 Query: 121 FYVNQVVVQRMIAQNYGRIVNIASIAGKEGNPTASAYSAAKAGLIALTKSLGKETATKNI 180 FYVNQVVVQRMIAQNYGRIVNIASIAGKEGNPTASAYSAAKAG+IALTKSLGKETATKNI Sbjct: 121 FYVNQVVVQRMIAQNYGRIVNIASIAGKEGNPTASAYSAAKAGMIALTKSLGKETATKNI 180 Query: 181 AVNAITPAAAQTRILEQCTQAHIDYMLSKIPRGRFVKVEEIAAMVSWLVSEENSFTTASV 240 AVNAITPAAAQTRILEQCTQAHIDYMLSKIPR RFVKVEE+AAMV+WLVSEENSFTTASV Sbjct: 181 AVNAITPAAAQTRILEQCTQAHIDYMLSKIPRARFVKVEELAAMVAWLVSEENSFTTASV 240 Query: 241 FDLSGGRATY 250 FDLSGGRATY Sbjct: 241 FDLSGGRATY 250 >gi|300309919|ref|YP_003774011.1| 3-oxoacyl-(acyl-carrier protein) reductase [Herbaspirillum seropedicae SmR1] Length = 244 Score = 194 bits (494), Expect = 4e-51, Method: Compositional matrix adjust. Identities = 115/247 (46%), Positives = 151/247 (61%), Gaps = 10/247 (4%) Query: 4 YDFQGRSAIITGGAQGIGFAIAERLLQGKAKVVLWDLDEKLLQEARAKLESLGNVETVAV 63 YDF A++TGG GIG AI+ RL G A+VV+WD R + ++ Sbjct: 8 YDFGDCVAVVTGGHGGIGAAISARLAAGGAQVVVWDQHHDAASPYRQQ----------SI 57 Query: 64 NIADQAAVQRAIEATEKVTQSIDVLVHSAGIAGQNGLVADYSPEEWRRVIDIDLNGAFYV 123 +I D AV +A + T ID L+++AG AG + +Y EW R++ ++L G F+ Sbjct: 58 DITDAPAVMQAAQELLADTGRIDFLINNAGYAGPTVPLDEYDVGEWHRIVQVNLLGVFHA 117 Query: 124 NQVVVQRMIAQNYGRIVNIASIAGKEGNPTASAYSAAKAGLIALTKSLGKETATKNIAVN 183 + V M A GRIVNIAS+AGKEG P ASAYSAAKAG++ALTKSLGKE A + VN Sbjct: 118 CRSVTPAMRAARRGRIVNIASLAGKEGTPNASAYSAAKAGVLALTKSLGKELAESGVLVN 177 Query: 184 AITPAAAQTRILEQCTQAHIDYMLSKIPRGRFVKVEEIAAMVSWLVSEENSFTTASVFDL 243 AI PAA +T +L Q + AH+ M++K P R V+E+A M +WL S SF T +VFDL Sbjct: 178 AIAPAAVKTALLGQMSPAHVQTMIAKSPMHRLGSVDEVADMCAWLCSGSCSFNTGAVFDL 237 Query: 244 SGGRATY 250 SGGRATY Sbjct: 238 SGGRATY 244 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 131 bits (329), Expect = 6e-32, Method: Compositional matrix adjust. Identities = 82/243 (33%), Positives = 130/243 (53%), Gaps = 9/243 (3%) Query: 9 RSAIITGGAQGIGFAIAERLLQGKAKVVLW-----DLDEKLLQEARAKLESLGNVETVAV 63 R A +TGG GIG AI RL + VV +K L R E ++ Sbjct: 4 RIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMR---EQGFDIHASEG 60 Query: 64 NIADQAAVQRAIEATEKVTQSIDVLVHSAGIAGQNGLVADYSPEEWRRVIDIDLNGAFYV 123 N+++ + + A E +DVLV++AGI ++G S ++W VID +LN F V Sbjct: 61 NVSNWESTKAAFEKVRAEIGEVDVLVNNAGIT-RDGQFRKMSKDDWDAVIDTNLNSLFNV 119 Query: 124 NQVVVQRMIAQNYGRIVNIASIAGKEGNPTASAYSAAKAGLIALTKSLGKETATKNIAVN 183 + V++ M + +GRI+NI+S+ G++G + YS AKAG+ T +L +E ATK + VN Sbjct: 120 TKQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVN 179 Query: 184 AITPAAAQTRILEQCTQAHIDYMLSKIPRGRFVKVEEIAAMVSWLVSEENSFTTASVFDL 243 ++P T ++ ++ ++S IP R + EEIA++V+W+ S E + T S F L Sbjct: 180 TVSPGYIGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFSL 239 Query: 244 SGG 246 +GG Sbjct: 240 NGG 242 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 127 bits (318), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 80/248 (32%), Positives = 132/248 (53%), Gaps = 3/248 (1%) Query: 1 MNHYDFQGRSAIITGGAQGIGFAIAERLLQGKAKVVLWDLDEKLLQEARAKLESLGNV-- 58 M+ + QG+ A++TG ++GIG AIA L + AKV+ E L +LG Sbjct: 1 MSAQNLQGQVALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPEAG 60 Query: 59 ETVAVNIADQAAVQRAIEATEKVTQSIDVLVHSAGIAGQNGLVADYSPEEWRRVIDIDLN 118 +N+ D A +E +K S+ +LV++AGI Q+ L EEW VI +L Sbjct: 61 RGAVLNVNDAEASVALVEQVQKDFGSLSILVNNAGIT-QDQLAMRMKDEEWDSVIATNLT 119 Query: 119 GAFYVNQVVVQRMIAQNYGRIVNIASIAGKEGNPTASAYSAAKAGLIALTKSLGKETATK 178 +++ V++ M+ GRI+NI S+ G+ GNP Y+AAKAG+ ++++L +E ++ Sbjct: 120 AVGRLSRAVLRGMMKARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSR 179 Query: 179 NIAVNAITPAAAQTRILEQCTQAHIDYMLSKIPRGRFVKVEEIAAMVSWLVSEENSFTTA 238 NI VN + P T + + +L +IP GR EEIA V++L S + ++ T Sbjct: 180 NITVNCVAPGFIDTDMTRALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITG 239 Query: 239 SVFDLSGG 246 + +++GG Sbjct: 240 TTLNVNGG 247 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 115 bits (289), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 76/252 (30%), Positives = 131/252 (51%), Gaps = 11/252 (4%) Query: 1 MNHYDFQGRSAIITGGAQGIGFAIAERLLQGKAKVVLWDLDEKLLQEARAKLESLGN-VE 59 MN D R A++TGGA GIG +I+++L V++ D++ + L + G+ Sbjct: 1 MNRNDSGARVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAA 60 Query: 60 TVAVNIADQAAVQRAIEATEKVTQSIDVLVHSAGIAGQNGLVADYSPEEWRRVIDIDLNG 119 + +++ ++ A EK DVLV++AG+A + DY E W + +++++ G Sbjct: 61 PLQMDLGSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFL-DYPLENWLQTMNVNVTG 119 Query: 120 AFYVNQVVVQRMIAQNYGRIVNIASIAGKEGNPTASAYSAAKAGLIALTKSLGKETATKN 179 Q + M+ Q +GRIVNI+SI+G +AY +KA +I LT+ + E A Sbjct: 120 VLLAGQHAARLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYG 179 Query: 180 IAVNAITPAAAQTRIL-----EQCTQAHIDYMLSKIPRGRFVKVEEIAAMVSWLVSEENS 234 I VN++ P T + EQ +++ Y L +P R+ +EIA+ V +L +EE++ Sbjct: 180 ITVNSVAPGPVDTPMTQAMHSEQTRESY--YRL--VPMRRYGTTDEIASAVCYLAAEESA 235 Query: 235 FTTASVFDLSGG 246 + T V + GG Sbjct: 236 YITGHVIPVDGG 247 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.130 0.358 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 730,629 Number of extensions: 27447 Number of successful extensions: 269 Number of sequences better than 1.0e-08: 46 Number of HSP's gapped: 186 Number of HSP's successfully gapped: 46 Length of query: 250 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 167 Effective length of database: 1,226,865 Effective search space: 204886455 Effective search space used: 204886455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)