BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0123.p (300 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310596|ref|YP_003774688.1| 2-keto-4-pentenoate hydratase/2... 519 e-148 gi|300312873|ref|YP_003776965.1| hypothetical protein Hsero_3578... 109 3e-25 >gi|300310596|ref|YP_003774688.1| 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase [Herbaspirillum seropedicae SmR1] Length = 281 Score = 519 bits (1336), Expect = e-148, Method: Compositional matrix adjust. Identities = 246/281 (87%), Positives = 265/281 (94%) Query: 20 MKLLRYGPVGQEKPGILDQAGKIRDLSAHIKDVNGAALDDATLAKIRALDLQSLPVVDGN 79 MKLLRYGPVGQEKPGILDQAGKIRDLSAHIKDVNGA LDDA+L KIR LDL +LPVV+GN Sbjct: 1 MKLLRYGPVGQEKPGILDQAGKIRDLSAHIKDVNGAVLDDASLEKIRKLDLNTLPVVEGN 60 Query: 80 PRIGACVGNIGKFICIGLNYADHAAESGLPIPAEPVVFNKWTSAVVGPNDDVKIPRGSKK 139 PRIGACVGNIGKFICIGLNYADHAAES LPIPAEPVVFNKWTSAVVGPND+VKIPRGSKK Sbjct: 61 PRIGACVGNIGKFICIGLNYADHAAESNLPIPAEPVVFNKWTSAVVGPNDNVKIPRGSKK 120 Query: 140 TDWEVELGVVIGKAASYIEEKDALSHIAGYCVVNDVSEREYQTERGGTWDKGKGCDTFGP 199 TDWEVELGV+IGK SYI+EKDA+SH+AGYCVVNDVSEREYQ ERGGTWDKGKGCDTFGP Sbjct: 121 TDWEVELGVIIGKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIERGGTWDKGKGCDTFGP 180 Query: 200 IGPWLVTRDEIANPNNLQLWLEVDGKRYQNGNTNTMIFNVPFIISYLSKFMSLQPGDVIS 259 IGPWLVTRDE+A+P L +WLEVDGKRYQNGNT+TMIFNV I+SYLS+FMSLQPGDVIS Sbjct: 181 IGPWLVTRDEVADPQKLGMWLEVDGKRYQNGNTSTMIFNVAHIVSYLSRFMSLQPGDVIS 240 Query: 260 TGTPPGVGMGVKPNAVYLTAGQTMRLSIEGLGEQQQKTVNA 300 TGTPPGVGMGVKP AVYL AGQT+RL I+GLGEQ+QKT++A Sbjct: 241 TGTPPGVGMGVKPEAVYLRAGQTIRLGIDGLGEQEQKTIDA 281 >gi|300312873|ref|YP_003776965.1| hypothetical protein Hsero_3578 [Herbaspirillum seropedicae SmR1] Length = 227 Score = 109 bits (272), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 70/194 (36%), Positives = 99/194 (51%), Gaps = 5/194 (2%) Query: 82 IGACVGNIGKFICIGLNYADHAAESGL-PIPAEPVVFNKWTSAVVGPNDDVKIPRGSKKT 140 +G + + C+G NYA HA E G P P F K AVV + V P + Sbjct: 16 VGGAAFPVRRIYCVGRNYAAHAREMGHDPDREPPFFFMKPADAVVPTDSVVPYPVATSDY 75 Query: 141 DWEVELGVVIGKAASYIEEKDALSHIAGYCVVNDVSEREYQTER---GGTWDKGKGCDTF 197 E+EL V + K I + AL + GY V D++ R+ Q++ G WD KG D Sbjct: 76 HHEIELVVALAKGGRNIPVEQALELVFGYAVGLDMTRRDIQSQAKKLGRPWDMAKGFDQS 135 Query: 198 GPIGPWLVTRDEIANPNNLQLWLEVDGKRYQNGNTNTMIFNVPFIISYLSKFMSLQPGDV 257 P P +V + +P+ +WL+V+G+ Q G+ +I++V ISYLS + L PGD+ Sbjct: 136 APCAP-IVPVAQAGHPDQGAVWLKVNGEVRQQGDLADLIWSVAETISYLSGLVELFPGDL 194 Query: 258 ISTGTPPGVGMGVK 271 I TGTP GVG VK Sbjct: 195 IFTGTPEGVGAVVK 208 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.137 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,064,967 Number of extensions: 48650 Number of successful extensions: 139 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 137 Number of HSP's successfully gapped: 2 Length of query: 300 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 215 Effective length of database: 1,217,395 Effective search space: 261739925 Effective search space used: 261739925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)