BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0122.p (154 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 72 2e-14 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 68 3e-13 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 67 7e-13 gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 60 6e-11 gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herb... 60 1e-10 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 72.0 bits (175), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 50/147 (34%), Positives = 85/147 (57%), Gaps = 10/147 (6%) Query: 3 DETWENMRAVNVDAGFHLARELARPLIAAREPGSFLMLTSL--HASTPRNLPHYSTSKAA 60 DE W+++ A N+ A L+R + R ++ AR G + +TS+ A P + +Y+ +KA Sbjct: 107 DEEWDSVIATNLTAVGRLSRAVLRGMMKAR-TGRIINITSVVGEAGNPGQM-NYAAAKAG 164 Query: 61 LSMLVKELAKTLGRFGIRVNALVPGAI-----AAGGFVADPALTRHIPLGRLGQSEDLAP 115 ++ + + LA+ +G I VN + PG I A A+ + IPLGRLG +E++A Sbjct: 165 VAGMSRALAREIGSRNITVNCVAPGFIDTDMTRALNEQQSAAILQQIPLGRLGAAEEIA- 223 Query: 116 MAMAVLSNRLSAYVTGASFVVDGGMSL 142 A+A L++ +AY+TG + V+GGM + Sbjct: 224 YAVAFLASPQAAYITGTTLNVNGGMYM 250 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 68.2 bits (165), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 43/149 (28%), Positives = 80/149 (53%), Gaps = 11/149 (7%) Query: 1 VTDETWENMRAVNVDAGFHLARELARPLIAAREPGSFLMLTSLHASTPRNL---PHYSTS 57 +T + ++ + V++ ++++ + P + A++ GS + ++S+ A + PHYS + Sbjct: 100 ITGKDYDRILDVSLRGTLYMSQAVL-PAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAA 158 Query: 58 KAALSMLVKELAKTLGRFGIRVNALVPGAIAAG------GFVADPALTRHIPLGRLGQSE 111 KA + L + +A+ G GIRVN + PG I+ + + IPLGRLG + Sbjct: 159 KAGVLGLARAMAREFGPQGIRVNCITPGLISTDINKDILSAEKKAEIAQTIPLGRLGGPD 218 Query: 112 DLAPMAMAVLSNRLSAYVTGASFVVDGGM 140 D+ + + L++ LS Y TG + V+GGM Sbjct: 219 DVGGVCV-FLASDLSQYCTGVTLDVNGGM 246 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 67.0 bits (162), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 46/151 (30%), Positives = 80/151 (52%), Gaps = 15/151 (9%) Query: 1 VTDETWENMRAVNVDAGFHLARELARPLIAAREPGSFLMLTSLHASTPRNL---PHYSTS 57 +T ++ + V++ ++++ + P + A++ GS + ++S+ A + PHYS + Sbjct: 100 ITGADYDRILDVSLRGTLYMSQAVL-PAMRAQQNGSIICISSVSAQRGGGILGGPHYSAA 158 Query: 58 KAALSMLVKELAKTLGRFGIRVNALVPGAIAAGGFVADPALTRH--------IPLGRLGQ 109 KA + L + +A+ G GIRVN++ PG I G + LT IPL RLG+ Sbjct: 159 KAGVLGLARAMAREFGPEGIRVNSITPGLI--GTDIIKGKLTEEKKAEIAETIPLARLGR 216 Query: 110 SEDLAPMAMAVLSNRLSAYVTGASFVVDGGM 140 ++D+ A L + LS Y TG + V+GGM Sbjct: 217 ADDIGG-ACVFLGSDLSQYCTGITLDVNGGM 246 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 60.5 bits (145), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 48/163 (29%), Positives = 78/163 (47%), Gaps = 32/163 (19%) Query: 3 DETWENMRAVNVDAGFHLARELARPLIAAREPGSFLMLTSLHASTP--RNLPHYSTSKAA 60 D+ W+ +N A FH R + P + A++ GS + + S +S N Y SKAA Sbjct: 99 DKAWDFSFNLNAKAMFHTIRAV-LPGMLAKKAGSIVNIASAASSVKGVANRFAYGASKAA 157 Query: 61 LSMLVKELAKTLGRFGIRVNALVPGAIAAGGFVADPALTRHI------------------ 102 + L K +A GIR NA+ PG I + P+L + I Sbjct: 158 VVGLTKSVAADFVAQGIRCNAICPGTIES------PSLNQRISTQAKETGKSEEEVRAAF 211 Query: 103 ----PLGRLGQSEDLAPMAMAVLSNRLSAYVTGASFVVDGGMS 141 P+GR+G++E++A +A+ + S+ S + TG+ ++DGG S Sbjct: 212 VGRQPMGRIGKAEEVAALALYLASDE-SNFTTGSIHMIDGGWS 253 >gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 59.7 bits (143), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 53/149 (35%), Positives = 79/149 (53%), Gaps = 20/149 (13%) Query: 4 ETWENMRAVNVDAGFHLARELARPLIAAREPGSFLMLTSLHASTPRNLPH-----YSTSK 58 E + AVNV + F +E AR + E G + + S +A +P Y+ SK Sbjct: 117 EDLDRTLAVNVRSVFVATQEAARYM---GEGGRVISIGSTNAD---RIPFAGGAVYAMSK 170 Query: 59 AALSMLVKELAKTLGRFGIRVNALVPGAI------AAGGFVADPALTRHIPLGRLGQSED 112 +AL L K LA+ LG GI VN + PG + A+G F A +LT + L R G++E+ Sbjct: 171 SALVGLTKGLARDLGPRGITVNNVQPGPVDTDMNPASGDFAA--SLTGLMALPRYGKAEE 228 Query: 113 LAPMAMAVLSNRLSAYVTGASFVVDGGMS 141 +A +A L+ S Y+TGA+ ++DGG S Sbjct: 229 IASF-VAYLAGPESGYITGANLMIDGGFS 256 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 503,758 Number of extensions: 18864 Number of successful extensions: 146 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 150 Number of HSP's successfully gapped: 16 Length of query: 154 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 77 Effective length of database: 1,255,275 Effective search space: 96656175 Effective search space used: 96656175 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)