BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0119.p (294 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogen... 141 5e-35 gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogen... 140 2e-34 gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenas... 108 8e-25 gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum s... 82 5e-17 >gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 297 Score = 141 bits (356), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 102/299 (34%), Positives = 151/299 (50%), Gaps = 8/299 (2%) Query: 1 MTKKICFIGLGMMGYPMAALLTKAGHTVHVADADPARAAQFAAEYQAQAFDAAAAQLPPA 60 M+ I FIGLG MG PMA L KAG +V D A + D+ AA + A Sbjct: 1 MSANIVFIGLGNMGLPMAQNLVKAGFSVSGHDLVKASVDKLVEVGGKTEADSMAA-VAKA 59 Query: 61 DIVITMLPNSDIVESVVLGKNKDGLAFRLSTATFIDMSSSEPMRSRQLAADLAEIGHHYI 120 D+VITMLP S VES+ LG+ LA ID S+ P +R++AA G + Sbjct: 60 DVVITMLPASRHVESLYLGE-AGVLASAKPGTLLIDCSTIAPEAARKVAAAAKARGFEML 118 Query: 121 DAPVSGGVKKAIEGTLAIMVGGAATTYAACTEVLQCMGKTLFHVGGPGAGHAMKALNNFV 180 DAPVSGG A GTL MVGG+ + A LQ MGK ++H G G+G +K NN + Sbjct: 119 DAPVSGGTNGATAGTLTFMVGGSKEAFEAAQPYLQKMGKAIYHAGESGSGQTVKVCNNML 178 Query: 181 SAAGLVAAVEALHVGQIFGLDPAVMTDVLNTSTGRNNTTEAK------VKQFMLSGSFGS 234 ++ EA+ +G G+DP V++++++ S+GRN T E ++ S + Sbjct: 179 LGILMIGTSEAIRLGMANGMDPKVLSEIMSKSSGRNWTLEVYNPCPGVMETAPASKGYAG 238 Query: 235 GFSLQLMTKDLGVAMKLGQELNYPMQLGNECLKIWKDAAQSLDRVADHTEMYRLLADTR 293 GF + LM KDLG+A++ N + LG ++ + + D + ++ +L + Sbjct: 239 GFGVDLMLKDLGLAVENALATNCAIPLGATARNLYDIHSMNGAGKLDFSSIFNMLGKAQ 297 >gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 288 Score = 140 bits (352), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 101/287 (35%), Positives = 147/287 (51%), Gaps = 5/287 (1%) Query: 3 KKICFIGLGMMGYPMAALLTKAGHTVHVADADPARAAQFAAEYQAQAFDAAAAQLPPADI 62 KKI IGLG MG MA L + G+ V DA + A + D+ AA P ++ Sbjct: 2 KKIGVIGLGNMGRGMAFSLQRGGYEVLGYDAQESTRTALA-QQGLHCVDSIAALAPAVEM 60 Query: 63 VITMLPNSDIVESVVLGKNKDGLAFRLSTATFIDMSSSEPMRSRQLAADLAEIGHHYIDA 122 V+ LP SDIVE VV G+ L +D ++++P +R+LAA L G +DA Sbjct: 61 VVLSLPTSDIVEQVVEGEGGL-LQHGAPGLVIVDTTTADPNSTRRLAARLQAAGMELVDA 119 Query: 123 PVSGGVKKAIEGTLAIMVGGAATTYAACTEVLQCMGKTLFHVGGPGAGHAMKALNNFVSA 182 PVSGG K A T+ +++GG+A T A VL M HVG GAGH K LNN V+ Sbjct: 120 PVSGGPKGAATATMGMVIGGSAETVARIEPVLAVMSAKRVHVGPVGAGHVAKLLNNLVTG 179 Query: 183 AGLVAAVEALHVGQIFGLDPAVMTDVLNTSTGRNNTTEAKVKQFMLSGSFGSGFSLQLMT 242 L+ A EA + GLD + L + + E ++ + +F SGF+++LM Sbjct: 180 THLLIAGEAARIAAAAGLDARQIFQGLAAGSANSKVIEVFYPTWIFNEAFDSGFTMKLMR 239 Query: 243 KDLGVAMKLGQELNYPMQLGNECLKIWKDAAQSLDRVADHTEMYRLL 289 KDL +AM L +E L E ++W A+S++ +AD + R++ Sbjct: 240 KDLRLAMNLIEETRSTTPLAREAGRLW---ARSVEGLADAEDFNRIV 283 >gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 298 Score = 108 bits (269), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 79/263 (30%), Positives = 132/263 (50%), Gaps = 6/263 (2%) Query: 1 MTKKICFIGLGMMGYPMAALLTKAGHTVHVADADPARAAQFAAEYQAQAFDAAAAQLP-P 59 M++ + IGLG MGY +A+ L +AG VH D +FAA A A+L Sbjct: 1 MSRNVGVIGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVAC--ANPAELAGK 58 Query: 60 ADIVITMLPNSDIVESVVLGKNKDGLAFRLSTATFIDMSSSEPMRSRQLAADLAEIGHHY 119 D+VIT++ N+ E+V+ G N A + + I ++ P + +L L E G Sbjct: 59 VDVVITLVVNAAQTETVLFGDNGAVSAMKPGS-VVISSATVAPDFAIELGKRLVEKGLLL 117 Query: 120 IDAPVSGGVKKAIEGTLAIMVGGAATTYAACTEVLQCMGKTLFHVGGP-GAGHAMKALNN 178 +DAPVSGG +A G + +M G A YA +VL M ++ +G G G +K +N Sbjct: 118 LDAPVSGGAARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQ 177 Query: 179 FVSAAGLVAAVEALHVGQIFGLDPAVMTDVLNTSTGRNNTTEAKVKQFMLSGSFGSGFSL 238 ++ + A+ EA+ +G G++P + +V+ S G + E +V +LSG + ++ Sbjct: 178 LLAGVHIAASAEAMALGLREGVNPDALYEVITHSAGNSWMFENRVPH-ILSGDYTPLSAV 236 Query: 239 QLMTKDLGVAMKLGQELNYPMQL 261 + KDLG+ + + +P+ L Sbjct: 237 DIFVKDLGLVLDTARRSKFPLPL 259 >gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 292 Score = 82.0 bits (201), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 84/262 (32%), Positives = 128/262 (48%), Gaps = 6/262 (2%) Query: 4 KICFIGLGMMGYPMAALLTKAGHTVHVADADPARAAQFAAEYQAQAFDAAAAQLPPADIV 63 KI F+GLG MG+PMAA L +AG+ V + PA + A AQA A AD++ Sbjct: 2 KIAFLGLGTMGFPMAANLVRAGYAVRAWNRSPA-PVERLAALGAQAAATPAEAAADADVL 60 Query: 64 ITMLPNSDIVESVVLGKNKDGLAFRLSTATFIDMSSSEPMRSRQLAADLAEIGHHYIDAP 123 I+ML + D E+ +L LA A ++M++ + +LA G Y+ AP Sbjct: 61 ISMLADDDATETSLLDAGA--LAALRPGAIHVNMATISVALALRLAGLHQARGVAYVAAP 118 Query: 124 VSGGVKKAIEGTLAIMVGGAATTYAACTEVLQCMGKTLFHVG-GPGAGHAMKALNNFVSA 182 V G V A G L I+ G AA +L +G+ + +G P +A K NF+ A Sbjct: 119 VLGRVNVAEAGQLNILAAGEEQALAAVQPLLDVLGQKTWRLGRQPEQANAAKLAMNFMIA 178 Query: 183 AGLVAAVEALHVGQIFGLDPAVMTDVLNTSTGRNNTTEAKVKQFMLSGSFG-SGFSLQLM 241 + + A EA+ + Q +GLD A + L TST Q + F +GF L L Sbjct: 179 SAIGAMSEAVALVQGYGLDKAGFIE-LATSTAFAAPVYKGYGQAIADDRFEPAGFKLALG 237 Query: 242 TKDLGVAMKLGQELNYPMQLGN 263 KD+ +A++ G++ + P+ L + Sbjct: 238 LKDVRLALEAGEQAHVPLSLAS 259 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.132 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 751,735 Number of extensions: 25423 Number of successful extensions: 145 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 136 Number of HSP's successfully gapped: 4 Length of query: 294 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 210 Effective length of database: 1,222,130 Effective search space: 256647300 Effective search space used: 256647300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)