BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0099.p (227 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 128 4e-31 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 120 1e-28 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 119 3e-28 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 117 1e-27 gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogen... 115 2e-27 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 128 bits (321), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 79/219 (36%), Positives = 123/219 (56%), Gaps = 9/219 (4%) Query: 2 RLANKVVILTGAAGGVGRATARLLAREGAQVVVADKNPDGVRELAKEIGAASLGVTVDVS 61 RLA K V++T AA G+GRA+ L AREGA+V+ D + + ELA G + +DV+ Sbjct: 7 RLAGKTVLITAAAQGIGRASTELFAREGARVIATDISKPHLDELAGIAGVETH--LLDVT 64 Query: 62 QHASVKAMVAQTLAHFGRVDVLINNAGFGRVGTVETIEEEEWDSIIGVNLKGTFLCSKNV 121 A++KA+VA+ G +DVL N AG+ G + +++ WD +N K F + V Sbjct: 65 DDAAIKALVAK----IGTIDVLFNCAGYVAAGNILECDDKAWDFSFNLNAKAMFHTIRAV 120 Query: 122 IPLMAAAGGGSIVNVGSY-TAIAAIRERAAYIASKGGIAALTKAMALDHVGQKIRVNCVA 180 +P M A GSIVN+ S +++ + R AY ASK + LTK++A D V Q IR N + Sbjct: 121 LPGMLAKKAGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVAQGIRCNAIC 180 Query: 181 PGTIATPYFEKMFAXRARRWAAWRNRKKLPLPFCGWRRM 219 PGTI +P + + +A+ ++ +++ F G + M Sbjct: 181 PGTIESPSLNQRISTQAKETG--KSEEEVRAAFVGRQPM 217 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 120 bits (300), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 79/205 (38%), Positives = 118/205 (57%), Gaps = 10/205 (4%) Query: 1 MRLANKVVILTGAAG--GVGRATARLLAREGAQVVVAD---KNPDGVRELAKEIGAASLG 55 M L +KV I+TG AG G+G +TARL+A +GA++V+ D P G A +GA LG Sbjct: 1 MLLKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGA---AARLGAQHLG 57 Query: 56 VTVDVSQHASVKAMVAQTLAHFGRVDVLINNAGFGRVGTVETIEEEEWDSIIGVNLKGTF 115 + DV+ A+ +A A L FGR+D+L+NNAG + + I +++D I+ V+L+GT Sbjct: 58 LVADVTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGTL 117 Query: 116 LCSKNVIPLMAAAGGGSIVNVGSYTAI--AAIRERAAYIASKGGIAALTKAMALDHVGQK 173 S+ V+P M A GSIV + S +A I Y A+K G+ L +AMA + Q Sbjct: 118 YMSQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQG 177 Query: 174 IRVNCVAPGTIATPYFEKMFAXRAR 198 IRVNC+ PG I+T + + + + Sbjct: 178 IRVNCITPGLISTDINKDILSAEKK 202 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 119 bits (297), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 79/195 (40%), Positives = 112/195 (57%), Gaps = 6/195 (3%) Query: 1 MRLANKVVILTGAAG--GVGRATARLLAREGAQVVVADKNPDGVRELAKEIGAASLGVTV 58 M L +KV ++TG AG G+G ATARL+A +GA+VVV D A IG+ LG+ Sbjct: 1 MLLKDKVAVITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAARIGSQHLGLVA 60 Query: 59 DVSQHASVKAMVAQTLAHFGRVDVLINNAGFGR-VGTVETIEEEEWDSIIGVNLKGTFLC 117 DV+ AS + A L FGR+D+L+NNAG + V T+E I ++D I+ V+L+GT Sbjct: 61 DVTDKASCETAAAAILQQFGRIDILVNNAGITQPVKTLE-ITGADYDRILDVSLRGTLYM 119 Query: 118 SKNVIPLMAAAGGGSIVNVGSYTAI--AAIRERAAYIASKGGIAALTKAMALDHVGQKIR 175 S+ V+P M A GSI+ + S +A I Y A+K G+ L +AMA + + IR Sbjct: 120 SQAVLPAMRAQQNGSIICISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPEGIR 179 Query: 176 VNCVAPGTIATPYFE 190 VN + PG I T + Sbjct: 180 VNSITPGLIGTDIIK 194 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 117 bits (292), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 69/193 (35%), Positives = 105/193 (54%), Gaps = 2/193 (1%) Query: 1 MRLANKVVILTGAAGGVGRATARLLAREGAQVVVADKNPDGVRELAKEIGAASLGV-TVD 59 R KVVI+TGAA G+G ATAR + EGA+V++AD++ + ++ + D Sbjct: 10 QRFEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPERTAARETD 69 Query: 60 VSQHASVKAMVAQTLAHFGRVDVLINNAGFGRVGTVETIEEEEWDSIIGVNLKGTFLCSK 119 VS H V+ +V + FG++DVL+++AG G V + E+W + N+ G F ++ Sbjct: 70 VSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATNVNGVFYGAR 129 Query: 120 NVIPLMAAAGGGSIVNVGSYTAIAAIRERAAYIASKGGIAALTKAMALDHVGQKIRVNCV 179 +P + G IVNV S + +AA +AY ASKG + LT+AMALD + +R+N V Sbjct: 130 EALPHLEKT-RGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGVRINAV 188 Query: 180 APGTIATPYFEKM 192 P T M Sbjct: 189 CPSLTHTAMTADM 201 >gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 115 bits (289), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 67/204 (32%), Positives = 113/204 (55%), Gaps = 4/204 (1%) Query: 1 MRLANKVVILTGAAGGVGRATARLLAREGAQVVVADKNPDGVRELAKEI---GAASLGVT 57 M L +KV ++TG+A G+G+ A AR+GA+VV+AD + + A+EI G ++ V Sbjct: 1 MLLKDKVALITGSASGIGKEIAVEYARQGAKVVIADLALEAAKATAEEIVKEGGTAMAVA 60 Query: 58 VDVSQHASVKAMVAQTLAHFGRVDVLINNAGFGRVGTVETIEEEEWDSIIGVNLKGTFLC 117 ++V+ V VA + +G +D++I+NAG + V + E W ++ ++L G FL Sbjct: 61 MNVTDETQVDKGVADAVNTYGGLDIMISNAGIQIISPVAELSLENWRKMLAIHLDGAFLT 120 Query: 118 SKNVIPLMAAAG-GGSIVNVGSYTAIAAIRERAAYIASKGGIAALTKAMALDHVGQKIRV 176 ++ + M G GGSI+ +GS + A +A Y+ +K G+ L K +A + KIR Sbjct: 121 TRAAMKAMIKQGRGGSIIYMGSVHSHLASPLKAPYVTAKHGLLGLAKTVAKEGAKDKIRT 180 Query: 177 NCVAPGTIATPYFEKMFAXRARRW 200 N + PG + TP EK +A+ + Sbjct: 181 NVICPGFVRTPLVEKQIPEQAKEF 204 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 576,491 Number of extensions: 19925 Number of successful extensions: 202 Number of sequences better than 1.0e-08: 49 Number of HSP's gapped: 146 Number of HSP's successfully gapped: 49 Length of query: 227 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 145 Effective length of database: 1,231,600 Effective search space: 178582000 Effective search space used: 178582000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)