BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0098.p (164 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 192 2e-50 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 100 7e-23 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 97 5e-22 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 94 5e-21 gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semial... 83 1e-17 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 192 bits (487), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 86/158 (54%), Positives = 113/158 (71%) Query: 1 MSTLLINGQWVSPVNGGTIDVIDPCNGEVFTTIDRGSAADIDLAVKAAQGALDGAWGKLT 60 M I +WV+P +G +I V+DP +G+ F I RG+A DID AV+AA+ A +GAWG+L+ Sbjct: 1 MKQHFIANRWVAPTSGESIPVLDPSDGQPFDAIARGNATDIDQAVRAARLAYEGAWGRLS 60 Query: 61 ATERGRIMLRYAELILKDVEEIAQLEARDTGKPITTARNDAVAVARYFEFYGAAADKIHG 120 A ER R++++ A + EE+AQLE RD GKP+ AR DA A+ RYFEFY AADK+HG Sbjct: 61 AAERSRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARADAAAIVRYFEFYAGAADKLHG 120 Query: 121 ETIPYLNGYNVSVVRVPHGVTAHIIPWNYPAQMFGRTL 158 +TIPY GY V +R PHGVT HI+PWNYP Q+FGR + Sbjct: 121 DTIPYQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCV 158 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 100 bits (248), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 53/157 (33%), Positives = 85/157 (54%), Gaps = 2/157 (1%) Query: 4 LLINGQWVSPVNGGTIDVIDPCNGEVFTTIDRGSAADIDLAVKAAQGALDGAWGKLTATE 63 L ING+W + +G TIDVI+P EV I AD+D A++AA + W ++A E Sbjct: 7 LFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFE-TWRNMSAFE 65 Query: 64 RGRIMLRYAELILKDVEEIAQLEARDTGKPITTARNDAVAVARYFEFYGAAADKIHGETI 123 R +IM R A+L+ + EE+A+L + GKP+ A+ + + A +++ A + +G + Sbjct: 66 RSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLV 125 Query: 124 P-YLNGYNVSVVRVPHGVTAHIIPWNYPAQMFGRTLA 159 P + G V++ P G A PWN+P R L+ Sbjct: 126 PARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLS 162 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 97.4 bits (241), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 54/157 (34%), Positives = 84/157 (53%), Gaps = 2/157 (1%) Query: 5 LINGQWVSPVNGGTIDVIDPCNGEVFTTIDRGSAADIDLAVKAAQGALDGAWGKLTATER 64 +NGQW G + V +P NGE+ + AA+ A++AA A AW K TA ER Sbjct: 15 FVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWP-AWKKKTAKER 73 Query: 65 GRIMLRYAELILKDVEEIAQLEARDTGKPITTARNDAVAVARYFEFYGAAADKIHGETIP 124 I+ R+ EL+L + +++A + + GKP+ AR + A + E++ + +G+TIP Sbjct: 74 SAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTIP 133 Query: 125 YLNGYN-VSVVRVPHGVTAHIIPWNYPAQMFGRTLAP 160 + N + V++ GV A I PWN+PA M R P Sbjct: 134 SPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGP 170 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 94.4 bits (233), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 56/157 (35%), Positives = 77/157 (49%), Gaps = 2/157 (1%) Query: 5 LINGQWVSPVNGGTIDVIDPCNGEVFTTIDRGSAADIDLAVKAAQGALDGAWGKLTATER 64 I G++V PV G + I P G F + R SA D++LA+ AA A +WGK + TER Sbjct: 22 FIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAA-KKSWGKTSPTER 80 Query: 65 GRIMLRYAELILKDVEEIAQLEARDTGKPI-TTARNDAVAVARYFEFYGAAADKIHGETI 123 ++L+ A+ + ++E +A E D GKPI T D +F ++ AA G Sbjct: 81 ANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQEGSIC 140 Query: 124 PYLNGYNVSVVRVPHGVTAHIIPWNYPAQMFGRTLAP 160 P N P GV IIPWN+P M LAP Sbjct: 141 PIDNDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAP 177 >gi|300310458|ref|YP_003774550.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 493 Score = 83.2 bits (204), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 55/157 (35%), Positives = 86/157 (54%), Gaps = 2/157 (1%) Query: 5 LINGQWVSPVNGGTIDVIDPCNGEVFTTIDRGSAADIDLAVKAAQGALDGAWGKLTATER 64 LI W +G +DV DP G+VF ++ G AAD AV+AA A A +R Sbjct: 17 LIGADWRGAADGRQLDVSDPATGQVFASVPDGGAADARAAVEAAVAAFAAW-RATPAKQR 75 Query: 65 GRIMLRYAELILKDVEEIAQLEARDTGKPITTARNDAVAVARYFEFYGAAADKIHGETIP 124 I+ R+ +L+L +++ +L +R+ GKP+ A+ + A Y E++G A + +G+ IP Sbjct: 76 AGIIKRWNDLLLAHQDDLGRLISREQGKPLAEAKGEVAYAASYVEWFGEEATRANGDIIP 135 Query: 125 Y-LNGYNVSVVRVPHGVTAHIIPWNYPAQMFGRTLAP 160 + G + ++ P GV A I PWN+PA M R +AP Sbjct: 136 APVTGRRMMALKEPVGVVAAITPWNFPAAMIARKIAP 172 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 540,094 Number of extensions: 21765 Number of successful extensions: 102 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 85 Number of HSP's successfully gapped: 15 Length of query: 164 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 86 Effective length of database: 1,250,540 Effective search space: 107546440 Effective search space used: 107546440 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)