BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0097.p (300 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 347 7e-97 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 216 2e-57 gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrog... 206 2e-54 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 205 4e-54 gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 186 2e-48 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 347 bits (890), Expect = 7e-97, Method: Compositional matrix adjust. Identities = 166/287 (57%), Positives = 209/287 (72%) Query: 9 LAVEAGFPDGALNVVTGYGYEAGAALTAHPHINFATFTGSPEVGVLVQESAAKNHVPCVL 68 LA E GFP+GALN+VTGYG+EAG L HP +N +FTGSP VG LV +AA++H P L Sbjct: 186 LAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVNHISFTGSPNVGKLVVRAAAEHHTPVTL 245 Query: 69 ELGGKSPQIVFNDADLDKAVPVIIKAIVQNTGQTCAAGSRLLVQQDIYDKCIDRIAAGFN 128 ELGGKSPQIVF DAD++ +PV++ AIVQN GQTC+AGSRLLVQ+ Y++ ++R++ F+ Sbjct: 246 ELGGKSPQIVFEDADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQVLERLSGLFS 305 Query: 129 QVKVGTPAMDYDCGPIINKKQFDRVNKFIDGAKASGVPVLAEGTIGGNLSGNGYFVKPVL 188 Q++VG+ MD DCGP+I K Q +RV F+ A+ G+ + A+G I + G++ P L Sbjct: 306 QLQVGSSQMDLDCGPLIRKTQQERVAGFLSDAQRDGIALAAQGKIAAGVPTGGFYQAPAL 365 Query: 189 FGAVPRNHTLACDEVFGPVLSVIPFVDEEDAIDLANATDYGLTAGVWTENGSRQARMAKR 248 VP H LA +EVFGPVL+ +PF DE AI LAN TDYGL A VWT +G RQ R+A+ Sbjct: 366 LRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVASVWTRDGGRQMRLARA 425 Query: 249 VVSGQVFINCYGAGGGVELPFGGFKKSGHGREKGFMALEEFSTTKTI 295 + SGQVFIN YGAGGGVELPFGG K SGHGREKGF AL F+ KTI Sbjct: 426 IRSGQVFINNYGAGGGVELPFGGVKASGHGREKGFEALYGFTVLKTI 472 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 216 bits (549), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 124/290 (42%), Positives = 166/290 (57%), Gaps = 5/290 (1%) Query: 8 RLAVEAGFPDGALNVVTGYGYEAGAALTAHPHINFATFTGSPEVGVLVQESAAKNHVPCV 67 RLA EAGFPDG +NVVTGYG G AL HP + +FTGSPE G +Q A + Sbjct: 194 RLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQRIAGEQFKRVT 253 Query: 68 LELGGKSPQIVFNDADLDKAVPVIIKAIVQNTGQTCAAGSRLLVQQDIYDKCIDRIAAGF 127 LELGGKSPQIVF+DA + A+ + N GQ CAAGSR+LVQ+ + + +A Sbjct: 254 LELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQRFAKALAEAA 313 Query: 128 NQVKVGTPAMD-YDCGPIINKKQFDRVNKFIDGAKASGVPVLAEGTIGGNLSGNGYFVKP 186 + VG P+ GP+ K QF+RVN++I G +LA G + G+FV+P Sbjct: 314 AGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGV---SRPDKGWFVQP 370 Query: 187 VLFGAVPRNHTLACDEVFGPVLSVIPFVDEEDAIDLANATDYGLTAGVWTENGSRQARMA 246 +F ++A +E+FGPV ++I F EE+AI LAN + YGL A VWT N +R R+A Sbjct: 371 TIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWTTNLARAHRVA 430 Query: 247 KRVVSGQVFINCYGAGGGVELPFGGFKKSGHGREKGFMALEEFSTTKTIV 296 V G V +NC+ + LP+GG K SG GRE G ++ KT+ Sbjct: 431 AGVKVGAVGVNCW-SPLDANLPWGGIKDSGMGREGGLAGALAYTEEKTVT 479 >gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 504 Score = 206 bits (523), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 116/289 (40%), Positives = 166/289 (57%), Gaps = 5/289 (1%) Query: 8 RLAVEAGFPDGALNVVTGYGYEAGAALTAHPHINFATFTGSPEVGVLVQESAAKNHVPCV 67 RL +AG P G +NV+ G G A + F GSP G L+ ++AA +PCV Sbjct: 204 RLGEQAGLPAGLVNVLAGLGQTMVPQAMATWTVKKVIFVGSPATGALIAKAAAARVMPCV 263 Query: 68 LELGGKSPQIVFNDADLDKAVPVIIKAIVQNTGQTCAAGSRLLVQQDIYDKCIDRIAAGF 127 LELGGKS I+F DADL A AI GQ+C AGSRLLVQ+ +YD+ ++ +AAG Sbjct: 264 LELGGKSANIIFEDADLRLAAFGAQAAIFSGAGQSCVAGSRLLVQRKVYDRFVETVAAGA 323 Query: 128 NQVKVGTPAMD-YDCGPIINKKQFDRVNKFIDGAKASGVPVLAEGTIGGNLSGNGYFVKP 186 ++++G P D + GPI N+KQ++ + + + +G + A T G GYFV+P Sbjct: 324 EKIRLGAPLDDSTEVGPINNRKQYEHIQRMVARGLEAGATLAAGHTRYGE---EGYFVRP 380 Query: 187 VLFGAVPRNHTLACDEVFGPVLSVIPFVDEEDAIDLANATDYGLTAGVWTENGSRQARMA 246 L +A E+FGPV IPF DEE+AI +AN +++GL VWT + +R R+A Sbjct: 381 TLLAHASNAMEVARSEIFGPVAVAIPFEDEEEAIAIANDSEFGLAGAVWTRDVARAHRVA 440 Query: 247 KRVVSGQVFINCYGAGGGVELPFGGFKKSGHGREKGFMALEEFSTTKTI 295 V +G ++N Y V PFGGF +SG+GR G AL +++ TK++ Sbjct: 441 ASVNAGTFWVNSYKT-INVASPFGGFNRSGYGRSSGMEALYDYTQTKSV 488 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 205 bits (522), Expect = 4e-54, Method: Compositional matrix adjust. Identities = 116/289 (40%), Positives = 170/289 (58%), Gaps = 3/289 (1%) Query: 12 EAGFPDGALNVVTGYGYEAGAALTAHPHINFATFTGSPEVGVLVQESAAKNHVPCVLELG 71 EAG P G N+V G G E GAAL+AHP ++ + TGS GVLV ++AA ELG Sbjct: 199 EAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVTVKRVAQELG 258 Query: 72 GKSPQIVFNDADLDKAVPVIIKAIVQNTGQTCAAGSRLLVQQDIYDKCIDRIAAGFNQVK 131 GKSP +V DADL++A+P + A ++N GQ+C+A +RL+V + + + A Q+K Sbjct: 259 GKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVHELALATLAQMK 318 Query: 132 VGTPA-MDYDCGPIINKKQFDRVNKFIDGAKASGVPVLAEGTIGGNLSGNGYFVKPVLFG 190 VG P GP+ N+ QF RV + I+ G +LA G + GY+ +P +F Sbjct: 319 VGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDGLAQGYYARPTIFS 378 Query: 191 AVPRNHTLACDEVFGPVLSVIPFVDEEDAIDLANATDYGLTAGVWTENGSRQARMAKRVV 250 V + +A +E+FGPVL+++P+ E+AI +AN T YGL A V + + +A R+ Sbjct: 379 EVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRDKDQVRAVAARIQ 438 Query: 251 SGQVFINCYGAGGGVELPFGGFKKSGHGREKGFMALEEFSTTKTIVHFY 299 SGQV +N Y A + PFGG+K+SG+GRE G +EE+ K I+ +Y Sbjct: 439 SGQVHLN-YPAWDP-QAPFGGYKQSGNGREYGVEGMEEYMEVKAILGYY 485 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 186 bits (473), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 115/296 (38%), Positives = 168/296 (56%), Gaps = 16/296 (5%) Query: 16 PDGALNVVTGYGYEAGAALTAHPHINFATFTGSPEVGVLVQESAAKNHVPCVLELGGKSP 75 P G +N+V G+G EAG L ++ I FTG G L+ + A++N +P LELGGKSP Sbjct: 209 PPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYASQNLIPVTLELGGKSP 268 Query: 76 QIVFNDADLDKAVPVIIKAI------VQNTGQTCAAGSRLLVQQDIYDKCIDRIAAGFNQ 129 I F D LDK KA+ N G+ C SR+LVQ+ IY++ I+R Sbjct: 269 NIFFADV-LDKDDDFFDKALEGFAMFALNQGEVCTCPSRVLVQESIYERFIERALKRVAA 327 Query: 130 VKVGTPAMDYDC--GPIINKKQFDRVNKFIDGAKASGVPVLAEG---TIGGNLSGNGYFV 184 +K G P +D G +++Q +++ +ID K G VLA G +GG+L+ +GY+V Sbjct: 328 IKQGNP-LDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGGGREELGGDLA-SGYYV 385 Query: 185 KPVLFGAVPRNHTLACDEVFGPVLSVIPFVDEEDAIDLANATDYGLTAGVWTENGSRQAR 244 KP +F + +E+FGPV+SV F DEE+A+ +AN T YGL AG+WT +G+R R Sbjct: 386 KPTVFQGNNKMRIFQ-EEIFGPVVSVTTFKDEEEALAIANDTLYGLGAGLWTRDGTRAFR 444 Query: 245 MAKRVVSGQVFINCYGAGGGVELPFGGFKKSGHGREKGFMALEEFSTTKTIVHFYD 300 M + + +G+V+ NCY FGG+K+SG GRE M L+ + TK ++ Y Sbjct: 445 MGREIQAGRVWTNCYHLYPA-HAAFGGYKQSGIGRENHKMMLDHYQQTKNLLVSYS 499 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.139 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,033,087 Number of extensions: 46900 Number of successful extensions: 189 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 140 Number of HSP's successfully gapped: 19 Length of query: 300 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 215 Effective length of database: 1,217,395 Effective search space: 261739925 Effective search space used: 261739925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)