BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0092.p (350 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311178|ref|YP_003775270.1| lipase [Herbaspirillum seropedi... 479 e-136 gi|300311659|ref|YP_003775751.1| esterase/lipase [Herbaspirillum... 132 4e-32 gi|300309659|ref|YP_003773751.1| esterase-lipase superfamily pro... 110 2e-25 >gi|300311178|ref|YP_003775270.1| lipase [Herbaspirillum seropedicae SmR1] Length = 340 Score = 479 bits (1233), Expect = e-136, Method: Compositional matrix adjust. Identities = 239/339 (70%), Positives = 269/339 (79%), Gaps = 2/339 (0%) Query: 12 MKTLTSTLALSVLTLAVVNASAADLPGVEQNVAKFLQSLE--GSKTLDTMTPAEARSVLV 69 M + A++ + L NA AA GVE FLQ+LE G K L+ ++PA+AR+VLV Sbjct: 1 MTSFKQLAAVAAIGLVFGNAYAAGDAGVEPTTQAFLQALEAGGGKPLEQLSPADARAVLV 60 Query: 70 AAQAASKVTLPAADVSEKTIRVNGSDLKLTIVRPAGSKQKTLPAFMYFHGGGWVLGDFPT 129 AQA+ K+ LP ADVSEKTI +G +KLTIVRPAGS +KTLPAFM+FHGGGWVLGDFPT Sbjct: 61 GAQASVKLQLPKADVSEKTISADGQQIKLTIVRPAGSAKKTLPAFMFFHGGGWVLGDFPT 120 Query: 130 HERLVRDLVAGSGAVAVFVNYALSPEAEYGVAIEQAYAATKWVAEHGKEINIDGKRLGVA 189 HERLVRDLVA SGAVAVFVNY SPEA YGVAI QAYAATKWVAEHGKEIN+DGKRL VA Sbjct: 121 HERLVRDLVANSGAVAVFVNYTPSPEAHYGVAINQAYAATKWVAEHGKEINVDGKRLAVA 180 Query: 190 GNSAGGNIAAVVALMASEKGTPALRSQVLLSPVTDANFDTPSYKQFANGYFLTKDLMVWF 249 GNS GGN+AAVVALMA +KG PALRS+VLL PVTDANF+T SY QFANGYFLTK++M WF Sbjct: 181 GNSVGGNMAAVVALMAKDKGGPALRSEVLLWPVTDANFETASYNQFANGYFLTKNMMKWF 240 Query: 250 WDNYTTDAKARKQIYASPLQATSEQLKGLPPTLILTAEFDVLRDEAEAYGRKLDVAGVPV 309 WD+YTTDA RK+I ASPL AT QL GLPPTLI TAE DVLRDE E YGRKL AGV V Sbjct: 241 WDSYTTDAAKRKEITASPLNATPAQLSGLPPTLIQTAEKDVLRDEGEEYGRKLAAAGVAV 300 Query: 310 KSVRYNGMIHDFGLLNAFSHLPAPRSAMAQASQELKTNL 348 SVRYNGMIHDFGLLN + +PA ++AM QA +ELK L Sbjct: 301 TSVRYNGMIHDFGLLNVLAKVPAVQTAMRQAGEELKFRL 339 >gi|300311659|ref|YP_003775751.1| esterase/lipase [Herbaspirillum seropedicae SmR1] Length = 317 Score = 132 bits (332), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 76/209 (36%), Positives = 109/209 (52%), Gaps = 5/209 (2%) Query: 114 FMYFHGGGWVLGDFPTHERLVRDLVAGSGAVAVFVNYALSPEAEYGVAIEQAYAATKWVA 173 +YFHGGG+ +G +H+ L L A + + V+Y L+PE + A E A A W+ Sbjct: 87 LLYFHGGGYCVGSLDSHDTLCSALAARTPCCVLAVDYRLAPEHRFPTAFEDAADAYDWLL 146 Query: 174 EHGKEINIDGKRLGVAGNSAGGN-IAAVVALMASEKGTPALRSQVLLSPVTDANFDTPSY 232 +HG + + RL V G+SAGG AA+ P L QVLL P T A+ D S+ Sbjct: 147 QHGPALGLATNRLAVGGDSAGGTLAAALALRARQAARAPCL--QVLLYPCTSAHQDGDSH 204 Query: 233 KQFANGYFLTKDLMVWFWDNYTTDAKARKQIYASPLQATSEQLKGLPPTLILTAEFDVLR 292 ++FA GY L + + W + + R+ +PLQA + G P L++ + D L Sbjct: 205 RRFATGYLLEGETLQWMFGHTLNGDHERQDWRFAPLQAAD--VSGAAPALVVIGDHDPLL 262 Query: 293 DEAEAYGRKLDVAGVPVKSVRYNGMIHDF 321 DE AY KL AGVPV+ + GM+HDF Sbjct: 263 DEGMAYAEKLRQAGVPVQLDIHPGMVHDF 291 >gi|300309659|ref|YP_003773751.1| esterase-lipase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 332 Score = 110 bits (275), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 84/222 (37%), Positives = 114/222 (51%), Gaps = 14/222 (6%) Query: 103 PAGSKQKTLPAFMYFHGGGWVLGDFPTHERLVRDLVAGSGAVAVF-VNYALSPEAEYGVA 161 P QK P MYFHGGG+V G + R +A G +AV V YAL+P + A Sbjct: 76 PLARGQKR-PLVMYFHGGGFVSGSLEDADTPAR-FIAEHGQLAVLSVGYALAPAKPFPAA 133 Query: 162 IEQAYAATKWVAEHGKEINIDGKRLGVAGNSAGGNIAAVVALMASEKGTPALRSQVLLSP 221 E A+AA W ++ E+ +D R+ VAG+ AGG +AA + LM ++ L +QVL+SP Sbjct: 134 PEDAFAAATWACKNATELKVDLDRMVVAGDDAGGCLAASLTLMTRDRCGKPLAAQVLISP 193 Query: 222 VTDANFDTPSYKQFANGYFLTKDL----MVWFWDNYTTDAKARKQIYASPLQATSEQLKG 277 + D PS + + L DL + Y R YA+PL+ + +L G Sbjct: 194 MLD-----PSMRLLGDAARLQSDLTAETCAASYRQYLPQTMQRLHPYAAPLEVS--RLAG 246 Query: 278 LPPTLILTAEFDVLRDEAEAYGRKLDVAGVPVKSVRYNGMIH 319 LPP I TAE DVL EAE Y L AGVP + R+ G+ H Sbjct: 247 LPPAFIATAERDVLCPEAEKYASSLIRAGVPTQVSRFAGITH 288 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.130 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,022,862 Number of extensions: 43622 Number of successful extensions: 181 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 175 Number of HSP's successfully gapped: 5 Length of query: 350 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 264 Effective length of database: 1,212,660 Effective search space: 320142240 Effective search space used: 320142240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)