BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0083.p (263 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 142 2e-35 gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 136 1e-33 gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydroge... 122 3e-29 gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogena... 120 2e-28 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 120 2e-28 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 142 bits (359), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 91/254 (35%), Positives = 133/254 (52%), Gaps = 11/254 (4%) Query: 14 FRMDGRTALITGASKGLGEAMAEALAEQGAKVVLVARTTSELETVAARIRSQGGQAEVRT 73 F ++G+ ALITG+S G+G A+A +A+ GA +VL R +L VAA++R++G + Sbjct: 15 FSLEGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVAAQLRAEGHTIHESS 74 Query: 74 CDVTDRKAIRAVIASLEE----LDILINNAGSNIPEPIDVVTDEHLDYLQDLNTTAYFVV 129 D T + ++A + +E + IL+NNAG P++ + L N + F Sbjct: 75 FDATSSEEVKAAVQKIESEIGPIGILVNNAGIQRRNPLEEFKESDWHDLISTNLNSVFFA 134 Query: 130 AQAAVKKMKEHPDRKNRGA-SIINIASQMGHIGSESRTVYCMTKHAVVGLTRAMAVELAA 188 AQA + M RGA IINI S +G Y TK AV T+ MA++ Sbjct: 135 AQAVARHMIP------RGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAIDWGK 188 Query: 189 TGIRVNSIGPTFIDTPLVRRVVDTPEKYNTLVSRIPMGHMGQVQDIAAAAVYLASSASGM 248 GI+VN +GP + T + +V+ + + LV R P G V+D+ AAV+LAS AS Sbjct: 189 YGIQVNGLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDASRF 248 Query: 249 VTGAGLLVDGGWTA 262 V G L VDGG TA Sbjct: 249 VNGHILYVDGGVTA 262 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 136 bits (343), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 91/259 (35%), Positives = 138/259 (53%), Gaps = 24/259 (9%) Query: 15 RMDGRTALITGASKGLGEAMAEALAEQGAKVVLVARTTSELETVAARIRSQGGQAEVRT- 73 R+ G+T LIT A++G+G A E A +GA+V+ + L+ +A G A V T Sbjct: 7 RLAGKTVLITAAAQGIGRASTELFAREGARVIATDISKPHLDELA-------GIAGVETH 59 Query: 74 -CDVTDRKAIRAVIASLEELDILINNAGSNIPEPIDVVTDEHLDYLQDLNTTAYFVVAQA 132 DVTD AI+A++A + +D+L N AG I D+ D+ +LN A F +A Sbjct: 60 LLDVTDDAAIKALVAKIGTIDVLFNCAGYVAAGNILECDDKAWDFSFNLNAKAMFHTIRA 119 Query: 133 AVKKMKEHPDRKNRGASIINIASQMGHI-GSESRTVYCMTKHAVVGLTRAMAVELAATGI 191 + M + SI+NIAS + G +R Y +K AVVGLT+++A + A GI Sbjct: 120 VLPGM-----LAKKAGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVAQGI 174 Query: 192 RVNSIGPTFIDTPLVRRVVDTPEKYN---------TLVSRIPMGHMGQVQDIAAAAVYLA 242 R N+I P I++P + + + T K V R PMG +G+ +++AA A+YLA Sbjct: 175 RCNAICPGTIESPSLNQRISTQAKETGKSEEEVRAAFVGRQPMGRIGKAEEVAALALYLA 234 Query: 243 SSASGMVTGAGLLVDGGWT 261 S S TG+ ++DGGW+ Sbjct: 235 SDESNFTTGSIHMIDGGWS 253 >gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 241 Score = 122 bits (306), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 86/245 (35%), Positives = 132/245 (53%), Gaps = 9/245 (3%) Query: 18 GRTALITGASKGLGEAMAEALAEQGAKVVLVARTTSELETVAARIRSQGGQAEVRTCDVT 77 G+T +ITGA +G+G A A LA++GA+V+ +ART S+L AR++ + G A V D++ Sbjct: 5 GKTVIITGAGRGIGRACALLLAQRGAQVIALARTASDL----ARLQEEIG-ARVIAVDLS 59 Query: 78 DRKAIRAVIASLEELDILINNAGSNIPEPIDVVTDEHLDYLQDLNTTAYFVVAQAAVKKM 137 D A R +A D LIN+AG N+ E + ++DE + + +N A + QA +M Sbjct: 60 DPAAARRAMAEAGTADFLINSAGINVLESVLEMSDEGYEAVLGVNLRAALITCQA-FARM 118 Query: 138 KEHPDRKNRGASIINIASQMGHIGSESRTVYCMTKHAVVGLTRAMAVELAATGIRVNSIG 197 + + G +++NI S GH G E Y +K + G TR +A EL GIRVN++ Sbjct: 119 RIA---QGGGGAMVNITSIAGHRGFEQHLCYAASKAGLEGATRVLARELGPHGIRVNAVA 175 Query: 198 PTFIDTPLVRRVVDTPEKYNTLVSRIPMGHMGQVQDIAAAAVYLASSASGMVTGAGLLVD 257 PT T L P K +++R P +D+A + L S + M+ GA + VD Sbjct: 176 PTITLTELAEAAWSDPAKSGPMMARHPSARFASPEDVARSIAMLLSPDAAMLNGAVVPVD 235 Query: 258 GGWTA 262 GG+ A Sbjct: 236 GGFLA 240 >gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 120 bits (300), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 81/249 (32%), Positives = 129/249 (51%), Gaps = 11/249 (4%) Query: 15 RMDGRTALITGASKGLGEAMAEALAEQGAKVVLVARTTSELETVAARIRSQGGQAEVRTC 74 +++G+ AL+TG + G+G A A+ LA QGA+V + R +EL+ A I + Sbjct: 4 QLNGKIALVTGGTSGIGLATAQELAAQGAQVFITGRRQAELDAAVASIGAAATGIRADAS 63 Query: 75 DVTDRKAIRAVIA-SLEELDILINNAGSNIPEPIDVVTDEHLDYLQDLNTTAYFVVAQAA 133 +++ + A IA S LDIL NAG P+ +T+EH D + N Q A Sbjct: 64 VLSELDTVYAQIAKSAGRLDILFANAGGGDMMPLGAITEEHFDRIFGTNVRGVLFTVQKA 123 Query: 134 VKKMKEHPDRKNRGASIINIASQMGHIGSESRTVYCMTKHAVVGLTRAMAVELAATGIRV 193 + + + GAS+I +S +G+ + +VY +K AV R+ A++L IRV Sbjct: 124 LPLLVD-------GASVILTSSTTSVLGTANFSVYSASKAAVRNFARSWALDLKDRAIRV 176 Query: 194 NSIGPTFIDTPLVRRVVDTPEK---YNTLVSRIPMGHMGQVQDIAAAAVYLASSASGMVT 250 N + P I TP + +V + ++ L S++P+G +G+ Q+I A +LAS A+ V Sbjct: 177 NVVSPGPIRTPGLGELVPDDARQGLFDYLASQVPLGRLGEPQEIGKAVAFLASDAASFVN 236 Query: 251 GAGLLVDGG 259 G L VDGG Sbjct: 237 GIELFVDGG 245 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 120 bits (300), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 83/249 (33%), Positives = 135/249 (54%), Gaps = 12/249 (4%) Query: 16 MDGRTALITGASKGLGEAMAEALAEQGAKVVLVARTTSELETVAARIRSQGGQA-EVRTC 74 + G+ AL+TGAS+G+G A+A LA QGAKV+ A + S + + + G +A Sbjct: 6 LQGQVALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPEAGRGAVL 65 Query: 75 DVTDRKAIRAVIASLEE----LDILINNAGSNIPEPIDVVTDEHLDYLQDLNTTAYFVVA 130 +V D +A A++ +++ L IL+NNAG + + DE D + N TA ++ Sbjct: 66 NVNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRLS 125 Query: 131 QAAVKKMKEHPDRKNRGASIINIASQMGHIGSESRTVYCMTKHAVVGLTRAMAVELAATG 190 +A ++ M K R IINI S +G G+ + Y K V G++RA+A E+ + Sbjct: 126 RAVLRGMM-----KARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRN 180 Query: 191 IRVNSIGPTFIDTPLVRRVVDTPEKYNTLVSRIPMGHMGQVQDIAAAAVYLASSASGMVT 250 I VN + P FIDT + R + ++ ++ +IP+G +G ++IA A +LAS + +T Sbjct: 181 ITVNCVAPGFIDTDMTRAL--NEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYIT 238 Query: 251 GAGLLVDGG 259 G L V+GG Sbjct: 239 GTTLNVNGG 247 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.130 0.359 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 666,626 Number of extensions: 22466 Number of successful extensions: 247 Number of sequences better than 1.0e-08: 48 Number of HSP's gapped: 163 Number of HSP's successfully gapped: 48 Length of query: 263 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 180 Effective length of database: 1,226,865 Effective search space: 220835700 Effective search space used: 220835700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)