BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0074.p (462 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 342 3e-95 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 338 4e-94 gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrog... 336 2e-93 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 295 5e-81 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 281 9e-77 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 342 bits (878), Expect = 3e-95, Method: Compositional matrix adjust. Identities = 200/448 (44%), Positives = 272/448 (60%), Gaps = 17/448 (3%) Query: 13 DAERAVEAASAALDGPWGKLTASARGLLMFRLADLIERDAEQLAQTEVRDNGKLIVE-MR 71 D E A++AA AA WGK + + R ++ ++AD +E + E LA E DNGK I E M Sbjct: 57 DVELALDAAHAAKKS-WGKTSPTERANMLLKIADRMEANLELLATAETLDNGKPIRETMA 115 Query: 72 AQVGYLPQYFRYFGGLSDKIEGAVLPIDKADTFNFTTHEPLGVCVAITAWNSPLLLAVNK 131 A + +FRYF EG++ PID DT+ + HEPLGV I WN P+L+AV K Sbjct: 116 ADIPLAIDHFRYFAAAVRTQEGSICPIDN-DTYAYHFHEPLGVVGQIIPWNFPILMAVWK 174 Query: 132 LAPGLAAGNTFVLKPSEFTSASAIELAKLFVEAGFPAGVLNVVTGFGNEVGARLVSHPKV 191 LAP LAAGN VLKP+E T AS + L +L + P GV+N+V GFG E G L S+ ++ Sbjct: 175 LAPALAAGNCVVLKPAEQTPASIMVLIELIADL-IPPGVVNIVQGFGVEAGKPLASNKRI 233 Query: 192 AKIGFTGSEIGGQRIYEAAAKGFKHVTLELGGKSANIVFADA--KLDDAVKGAISG--IF 247 AKI FTG G+ I + A++ VTLELGGKS NI FAD K DD A+ G +F Sbjct: 234 AKIAFTGETTTGRLIMQYASQNLIPVTLELGGKSPNIFFADVLDKDDDFFDKALEGFAMF 293 Query: 248 AAT-GQTCVAGSRLLVHESIHDEFVEKLVAYARKARMGDPKETATQVGPVTTGPQYLKIL 306 A G+ C SR+LV ESI++ F+E+ + + G+P + +T +G + Q KIL Sbjct: 294 ALNQGEVCTCPSRVLVQESIYERFIERALKRVAAIKQGNPLDKSTMIGAQASQEQLEKIL 353 Query: 307 EYIDIAKKEGATCVLGGKAASRPECG----DGQFVEPTIFTDVRNDMRIAQEEVFGPVLS 362 YIDI K+EGA + GG R E G G +V+PT+F N MRI QEE+FGPV+S Sbjct: 354 SYIDIGKQEGAKVLAGG---GREELGGDLASGYYVKPTVFQG-NNKMRIFQEEIFGPVVS 409 Query: 363 IIKFKDDDDAVRIANDSLYGLAAGVWTQDIRRAMSLHKRLKVGIVWVNMYRAVSYMSPFG 422 + FKD+++A+ IAND+LYGL AG+WT+D RA + + ++ G VW N Y + FG Sbjct: 410 VTTFKDEEEALAIANDTLYGLGAGLWTRDGTRAFRMGREIQAGRVWTNCYHLYPAHAAFG 469 Query: 423 GYKRSGVGRENGMEAIREYLQTKSVWIS 450 GYK+SG+GREN + Y QTK++ +S Sbjct: 470 GYKQSGIGRENHKMMLDHYQQTKNLLVS 497 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 338 bits (868), Expect = 4e-94, Method: Compositional matrix adjust. Identities = 193/431 (44%), Positives = 258/431 (59%), Gaps = 8/431 (1%) Query: 27 GPWGKLTASARGLLMFRLADLIERDAEQLAQTEVRDNGKLIVEMRAQ-VGYLPQYFRYFG 85 G W KL+ RG L+ +LA L+ERD + LA + R G+ +E R V + R Sbjct: 58 GAWSKLSGLQRGQLLHKLAALVERDTDLLADMDARAIGRSPMEPRMMDVPNAISHLRAAA 117 Query: 86 GLSDKIEGAVLPIDK---ADTFNFTTHEPLGVCVAITAWNSPLLLAVNKLAPGLAAGNTF 142 G ++++EG +P T ++T EP+GV AI WN+PL++ K+A LAAG T Sbjct: 118 GWANQMEGRTIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTV 177 Query: 143 VLKPSEFTSASAIELAKLFVEAGFPAGVLNVVTGFGNEVGARLVSHPKVAKIGFTGSEIG 202 V+KP+E T SA+ LA+L EAGFP GV+NVVTG+GN VG L HP VAKI FTGS Sbjct: 178 VIKPAEETPQSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEA 237 Query: 203 GQRIYEAAAKGFKHVTLELGGKSANIVFADAKLDDAVKGAISGIFAATGQTCVAGSRLLV 262 G+ I A + FK VTLELGGKS IVF DA +DA+ G G+F GQ C AGSR+LV Sbjct: 238 GRAIQRIAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILV 297 Query: 263 HESIHDEFVEKLVAYARKARMGDPKETATQVGPVTTGPQYLKILEYIDIAKKEGATCVLG 322 S+ F + L A +GDP + ++GPV Q+ ++ YI EGAT + G Sbjct: 298 QRSLAQRFAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAG 357 Query: 323 GKAASRPECGDGQFVEPTIFTDVRNDMRIAQEEVFGPVLSIIKFKDDDDAVRIANDSLYG 382 G SRP+ G FV+PTIF D N M IA+EE+FGPV +II F +++A+ +ANDS YG Sbjct: 358 G--VSRPD--KGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYG 413 Query: 383 LAAGVWTQDIRRAMSLHKRLKVGIVWVNMYRAVSYMSPFGGYKRSGVGRENGMEAIREYL 442 LAA VWT ++ RA + +KVG V VN + + P+GG K SG+GRE G+ Y Sbjct: 414 LAATVWTTNLARAHRVAAGVKVGAVGVNCWSPLDANLPWGGIKDSGMGREGGLAGALAYT 473 Query: 443 QTKSVWISTAS 453 + K+V + AS Sbjct: 474 EEKTVTVLLAS 484 >gi|300310293|ref|YP_003774385.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 504 Score = 336 bits (861), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 178/431 (41%), Positives = 256/431 (59%), Gaps = 5/431 (1%) Query: 29 WGKLTASARGLLMFRLADLIERDAEQLAQTEVRDNGKLIVEMRAQVGYLPQYFRYFGGLS 88 W L+ +ARG ++ + +I +AE LA+ E +GK I + RA++ + + F Y+ G + Sbjct: 75 WWALSHAARGRALYAVGAVIRAEAEPLARLEAISSGKPIRDCRAEMQKVAEMFEYYAGWA 134 Query: 89 DKIEGAVLPIDKADTFNFTTHEPLGVCVAITAWNSPLLLAVNKLAPGLAAGNTFVLKPSE 148 DK G V+P+ + N+T EP G + +T WN+P +L P LA GN +LKPSE Sbjct: 135 DKFYGEVIPVPSSH-LNYTRREPYGTVLQMTPWNAPAFTCGWQLGPALATGNAVLLKPSE 193 Query: 149 FTSASAIELAKLFVEAGFPAGVLNVVTGFGNEVGARLVSHPKVAKIGFTGSEIGGQRIYE 208 T S++ +A+L +AG PAG++NV+ G G + + ++ V K+ F GS G I + Sbjct: 194 LTPFSSLAIARLGEQAGLPAGLVNVLAGLGQTMVPQAMATWTVKKVIFVGSPATGALIAK 253 Query: 209 AAAKGFKHVTLELGGKSANIVFADAKLDDAVKGAISGIFAATGQTCVAGSRLLVHESIHD 268 AAA LELGGKSANI+F DA L A GA + IF+ GQ+CVAGSRLLV ++D Sbjct: 254 AAAARVMPCVLELGGKSANIIFEDADLRLAAFGAQAAIFSGAGQSCVAGSRLLVQRKVYD 313 Query: 269 EFVEKLVAYARKARMGDPKETATQVGPVTTGPQYLKILEYIDIAKKEGATCVLGGKAASR 328 FVE + A A K R+G P + +T+VGP+ QY I + + GAT G Sbjct: 314 RFVETVAAGAEKIRLGAPLDDSTEVGPINNRKQYEHIQRMVARGLEAGATLAAGHTRYGE 373 Query: 329 PECGDGQFVEPTIFTDVRNDMRIAQEEVFGPVLSIIKFKDDDDAVRIANDSLYGLAAGVW 388 +G FV PT+ N M +A+ E+FGPV I F+D+++A+ IANDS +GLA VW Sbjct: 374 ----EGYFVRPTLLAHASNAMEVARSEIFGPVAVAIPFEDEEEAIAIANDSEFGLAGAVW 429 Query: 389 TQDIRRAMSLHKRLKVGIVWVNMYRAVSYMSPFGGYKRSGVGRENGMEAIREYLQTKSVW 448 T+D+ RA + + G WVN Y+ ++ SPFGG+ RSG GR +GMEA+ +Y QTKSVW Sbjct: 430 TRDVARAHRVAASVNAGTFWVNSYKTINVASPFGGFNRSGYGRSSGMEALYDYTQTKSVW 489 Query: 449 ISTASETPDPF 459 + T++ PF Sbjct: 490 VETSASPATPF 500 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 295 bits (755), Expect = 5e-81, Method: Compositional matrix adjust. Identities = 172/438 (39%), Positives = 250/438 (57%), Gaps = 4/438 (0%) Query: 13 DAERAVEAASAALDGPWGKLTASARGLLMFRLADLIERDAEQLAQTEVRDNGKLIVEMRA 72 D ++AV AA A +G WG+L+A+ R L+ +LA ++ E+LAQ E RD GK + + RA Sbjct: 40 DIDQAVRAARLAYEGAWGRLSAAERSRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARA 99 Query: 73 QVGYLPQYFRYFGGLSDKIEGAVLPIDKADTFNFTTHEPLGVCVAITAWNSPLLLAVNKL 132 + +YF ++ G +DK+ G +P T T EP GV I WN P+ + + Sbjct: 100 DAAAIVRYFEFYAGAADKLHGDTIPYQAGYTV-LTLREPHGVTGHIVPWNYPMQIFGRCV 158 Query: 133 APGLAAGNTFVLKPSEFTSASAIELAKLFVEAGFPAGVLNVVTGFGNEVGARLVSHPKVA 192 LAAGNT V+KP+E S + +A+L E GFP G LN+VTG+G+E G LV HP V Sbjct: 159 GGALAAGNTCVVKPAEDACLSILRVAELAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVN 218 Query: 193 KIGFTGSEIGGQRIYEAAAKGFKHVTLELGGKSANIVFADAKLDDAVKGAISGIFAATGQ 252 I FTGS G+ + AAA+ VTLELGGKS IVF DA ++ + ++ I GQ Sbjct: 219 HISFTGSPNVGKLVVRAAAEHHTPVTLELGGKSPQIVFEDADMEAMLPVVVNAIVQNAGQ 278 Query: 253 TCVAGSRLLVHESIHDEFVEKLVAYARKARMGDPKETATQVGPVTTGPQYLKILEYIDIA 312 TC AGSRLLV +++ +E+L + ++G + GP+ Q ++ ++ A Sbjct: 279 TCSAGSRLLVQRGAYEQVLERLSGLFSQLQVGS-SQMDLDCGPLIRKTQQERVAGFLSDA 337 Query: 313 KKEGATCVLGGKAASRPECGDGQFVEPTIFTDVRNDMRIAQEEVFGPVLSIIKFKDDDDA 372 +++G GK A+ G G + P + DV R+AQEEVFGPVL+ + F+D+ A Sbjct: 338 QRDGIALAAQGKIAAGVPTG-GFYQAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHA 396 Query: 373 VRIANDSLYGLAAGVWTQDIRRAMSLHKRLKVGIVWVNMYRAVSYMS-PFGGYKRSGVGR 431 + +AN + YGL A VWT+D R M L + ++ G V++N Y A + PFGG K SG GR Sbjct: 397 IALANGTDYGLVASVWTRDGGRQMRLARAIRSGQVFINNYGAGGGVELPFGGVKASGHGR 456 Query: 432 ENGMEAIREYLQTKSVWI 449 E G EA+ + K++ I Sbjct: 457 EKGFEALYGFTVLKTIAI 474 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 281 bits (719), Expect = 9e-77, Method: Compositional matrix adjust. Identities = 163/434 (37%), Positives = 241/434 (55%), Gaps = 5/434 (1%) Query: 12 ADAERAVEAASAALDGPWGKLTASARGLLMFRLADLIERDAEQLAQTEVRDNGKLIVEMR 71 A+ RA+EAA+AA W K TA R ++ R +L+ + + LA + GK + E R Sbjct: 49 AETRRAIEAANAAWPA-WKKKTAKERSAILRRWYELMLANTDDLALIMTAEQGKPLAEAR 107 Query: 72 AQVGYLPQYFRYFGGLSDKIEGAVLPIDKADTFNFTTHEPLGVCVAITAWNSPLLLAVNK 131 ++GY + +F + G +P E +GVC AIT WN P + K Sbjct: 108 GEIGYAASFIEWFAEEGKRTYGDTIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRK 167 Query: 132 LAPGLAAGNTFVLKPSEFTSASAIELAKLFVEAGFPAGVLNVVTGFGNEVGARLVSHPKV 191 P LAAG VLKP+E T SA+ LA L AG PAGV +VVTG +G + S+P V Sbjct: 168 AGPALAAGCPMVLKPAEATPFSALALAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIV 227 Query: 192 AKIGFTGSEIGGQRIYEAAAKGFKHVTLELGGKSANIVFADAKLDDAVKGAISGIFAATG 251 KI FTGS G+ + + +A K ++LELGG + IVF DA LD AV+GAI+ + G Sbjct: 228 RKISFTGSTGVGKLLMQQSASSIKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAG 287 Query: 252 QTCVAGSRLLVHESIHDEFVEKLVAYARKARMGDPKETATQVGPVTTGPQYLKILEYIDI 311 QTCV +R+ V + ++D F KLV +K ++G E GP+ K+ +++ Sbjct: 288 QTCVCANRIYVQDGVYDAFAAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVAD 347 Query: 312 AKKEGATCVLGGKAASRPECGDGQFVEPTIFTDVRNDMRIAQEEVFGPVLSIIKFKDDDD 371 A +GA +LGGK R G F EPT+ DV M++A+EE FGP+ + +FK D++ Sbjct: 348 AVAKGARVLLGGK---RHALGH-SFFEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEE 403 Query: 372 AVRIANDSLYGLAAGVWTQDIRRAMSLHKRLKVGIVWVNMYRAVSYMSPFGGYKRSGVGR 431 + +AND+ +GLA+ +++DI R + + L+ G+V +N + ++PFGG K+SG+GR Sbjct: 404 VLALANDTEFGLASYFYSRDIGRIWRVAEGLESGMVGINTGLISNEVAPFGGVKQSGLGR 463 Query: 432 ENGMEAIREYLQTK 445 E I +YL K Sbjct: 464 EGSHYGIDDYLVVK 477 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.136 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,436,802 Number of extensions: 63130 Number of successful extensions: 222 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 176 Number of HSP's successfully gapped: 20 Length of query: 462 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 373 Effective length of database: 1,198,455 Effective search space: 447023715 Effective search space used: 447023715 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)