BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0073.p (259 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 144 7e-36 gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 135 3e-33 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 128 5e-31 gi|300313779|ref|YP_003777871.1| short-chain dehydrogenase [Herb... 126 2e-30 gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogen... 125 3e-30 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 144 bits (363), Expect = 7e-36, Method: Compositional matrix adjust. Identities = 85/256 (33%), Positives = 138/256 (53%), Gaps = 9/256 (3%) Query: 7 MPSFRVDGKTALVTGAGSGIGAAISSTLAGSGARVHMLSLNGEQLAATAAALRELGGDVH 66 + F ++GK AL+TG+ GIG A++ +A +GA + + + +LAA AA LR G +H Sbjct: 12 LQGFSLEGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVAAQLRAEGHTIH 71 Query: 67 AHACDVTDSD----AVRRLIDGLPGLDIVVNNAGTNIPEPFLEVSDAHLDFMCNLNVRAC 122 + D T S+ AV+++ + + I+VNNAG P E ++ + + N+ + Sbjct: 72 ESSFDATSSEEVKAAVQKIESEIGPIGILVNNAGIQRRNPLEEFKESDWHDLISTNLNSV 131 Query: 123 FVVSQAAVRKMLADPARVAKGGCVINVSSQMGHVGSPDRTVYCMNKHAIEGLTKAMAVEL 182 F +QA R M+ A G +IN+ S +G P Y K A++ TK MA++ Sbjct: 132 FFAAQAVARHMIPRGA-----GKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAIDW 186 Query: 183 AATGVRVNSIGPTFVDTPLIRRIVDTPEKHEFLVSKIPMGRMATMEDIAAAALYLASPAA 242 G++VN +GP + T + +V+ + ++LV + P R +ED+ AA++LAS A+ Sbjct: 187 GKYGIQVNGLGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDAS 246 Query: 243 AMVTGHCLLVDGGWTA 258 V GH L VDGG TA Sbjct: 247 RFVNGHILYVDGGVTA 262 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 135 bits (340), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 90/259 (34%), Positives = 131/259 (50%), Gaps = 24/259 (9%) Query: 11 RVDGKTALVTGAGSGIGAAISSTLAGSGARVHMLSLNGEQLAATAAALRELGG--DVHAH 68 R+ GKT L+T A GIG A + A GARV ++ L EL G V H Sbjct: 7 RLAGKTVLITAAAQGIGRASTELFAREGARVIATDISKPHL-------DELAGIAGVETH 59 Query: 69 ACDVTDSDAVRRLIDGLPGLDIVVNNAGTNIPEPFLEVSDAHLDFMCNLNVRACFVVSQA 128 DVTD A++ L+ + +D++ N AG LE D DF NLN +A F +A Sbjct: 60 LLDVTDDAAIKALVAKIGTIDVLFNCAGYVAAGNILECDDKAWDFSFNLNAKAMFHTIRA 119 Query: 129 AVRKMLADPARVAKGGCVINVSSQMGHV-GSPDRTVYCMNKHAIEGLTKAMAVELAATGV 187 + MLA K G ++N++S V G +R Y +K A+ GLTK++A + A G+ Sbjct: 120 VLPGMLAK-----KAGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVAQGI 174 Query: 188 RVNSIGPTFVDTPLIRRIVDTPEKHE---------FLVSKIPMGRMATMEDIAAAALYLA 238 R N+I P +++P + + + T K V + PMGR+ E++AA ALYLA Sbjct: 175 RCNAICPGTIESPSLNQRISTQAKETGKSEEEVRAAFVGRQPMGRIGKAEEVAALALYLA 234 Query: 239 SPAAAMVTGHCLLVDGGWT 257 S + TG ++DGGW+ Sbjct: 235 SDESNFTTGSIHMIDGGWS 253 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 128 bits (321), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 84/254 (33%), Positives = 139/254 (54%), Gaps = 12/254 (4%) Query: 7 MPSFRVDGKTALVTGAGSGIGAAISSTLAGSGARVHMLSLNGEQLAATAAALRELGGDVH 66 M + + G+ ALVTGA GIG AI++ LA GA+V + + AA L LG + Sbjct: 1 MSAQNLQGQVALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPEAG 60 Query: 67 AHAC-DVTDSDAVRRLIDGLP----GLDIVVNNAGTNIPEPFLEVSDAHLDFMCNLNVRA 121 A +V D++A L++ + L I+VNNAG + + + D D + N+ A Sbjct: 61 RGAVLNVNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTA 120 Query: 122 CFVVSQAAVRKMLADPARVAKGGCVINVSSQMGHVGSPDRTVYCMNKHAIEGLTKAMAVE 181 +S+A +R M+ A+ G +IN++S +G G+P + Y K + G+++A+A E Sbjct: 121 VGRLSRAVLRGMMK-----ARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALARE 175 Query: 182 LAATGVRVNSIGPTFVDTPLIRRIVDTPEKHEFLVSKIPMGRMATMEDIAAAALYLASPA 241 + + + VN + P F+DT + R + ++ ++ +IP+GR+ E+IA A +LASP Sbjct: 176 IGSRNITVNCVAPGFIDTDMTRAL--NEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQ 233 Query: 242 AAMVTGHCLLVDGG 255 AA +TG L V+GG Sbjct: 234 AAYITGTTLNVNGG 247 >gi|300313779|ref|YP_003777871.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 126 bits (316), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 82/247 (33%), Positives = 125/247 (50%), Gaps = 9/247 (3%) Query: 14 GKTALVTGAGSGIGAAISSTLAGSGARVHMLSLNGEQLAATAAALRELGGDVHAHACDVT 73 GK ++TG GSGIGAA+ G GA+V L + E A A+L++ C++ Sbjct: 19 GKRVVITGGGSGIGAALVEAFVGQGAQVCFLDIAAEPSQALVASLKDAPIAPRFFPCNLM 78 Query: 74 DSDAVRRLIDGLP----GLDIVVNNAGTNIPEPFLEVSDAHLDFMCNLNVRACFVVSQAA 129 + +A+R + G+DI++NNA + +V+ A+ D +N+R F +QA Sbjct: 79 NLEALRATFTEIETVMGGVDILINNAANDDRHKTADVTPAYWDERLAVNLRHQFFCAQAV 138 Query: 130 VRKMLADPARVAKGGCVINVSSQMGHVGSPDRTVYCMNKHAIEGLTKAMAVELAATGVRV 189 + M R K G ++N S H+G PD T+Y K IEG+T MA + GVRV Sbjct: 139 LPGM-----RERKQGVILNFGSISWHLGLPDLTLYMTAKAGIEGMTHGMARDFGRDGVRV 193 Query: 190 NSIGPTFVDTPLIRRIVDTPEKHEFLVSKIPMGRMATMEDIAAAALYLASPAAAMVTGHC 249 N+I P + TP + TPE+ +++ + D+AA AL+L+S + A TG Sbjct: 194 NAIIPGAIRTPRQTLLWHTPEEEAKILAAQCLPTRVDPHDVAALALFLSSDSGAKCTGRE 253 Query: 250 LLVDGGW 256 VD GW Sbjct: 254 YYVDAGW 260 >gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 125 bits (315), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 86/261 (32%), Positives = 135/261 (51%), Gaps = 22/261 (8%) Query: 15 KTALVTGAGSGIGAAISSTLAGSGARVHMLSLNGEQLAATAAALRELGGDVHAHACDVTD 74 K AL+TG+ SGIG I+ A GA+V + L E ATA + + GG A A +VTD Sbjct: 6 KVALITGSASGIGKEIAVEYARQGAKVVIADLALEAAKATAEEIVKEGGTAMAVAMNVTD 65 Query: 75 SDAVRR----LIDGLPGLDIVVNNAGTNIPEPFLEVSDAHLDFMCNLNVRACFVVSQAAV 130 V + ++ GLDI+++NAG I P E+S + M +++ F+ ++AA+ Sbjct: 66 ETQVDKGVADAVNTYGGLDIMISNAGIQIISPVAELSLENWRKMLAIHLDGAFLTTRAAM 125 Query: 131 RKMLADPARVAKGGCVINVSSQMGHVGSPDRTVYCMNKHAIEGLTKAMAVELAATGVRVN 190 + M+ + +GG +I + S H+ SP + Y KH + GL K +A E A +R N Sbjct: 126 KAMI----KQGRGGSIIYMGSVHSHLASPLKAPYVTAKHGLLGLAKTVAKEGAKDKIRTN 181 Query: 191 SIGPTFVDTPLIRRIVDTPEK-------HEFLVSKIPM-----GRMATMEDIAAAALYLA 238 I P FV TPL+ + + PE+ E ++ KI + G T +D+A A++LA Sbjct: 182 VICPGFVRTPLVEKQI--PEQAKEFGISEEDVIKKIMLKDTVDGEFTTTQDVAQTAVFLA 239 Query: 239 SPAAAMVTGHCLLVDGGWTAQ 259 + +TG ++V GW Q Sbjct: 240 AFPTNALTGQSVVVSHGWHMQ 260 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 684,839 Number of extensions: 24299 Number of successful extensions: 227 Number of sequences better than 1.0e-08: 49 Number of HSP's gapped: 162 Number of HSP's successfully gapped: 49 Length of query: 259 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 176 Effective length of database: 1,226,865 Effective search space: 215928240 Effective search space used: 215928240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)