BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0071.p (546 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314045|ref|YP_003778137.1| choline dehydrogenase [Herbaspi... 395 e-111 gi|300314074|ref|YP_003778166.1| choline dehydrogenase [Herbaspi... 389 e-109 >gi|300314045|ref|YP_003778137.1| choline dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 543 Score = 395 bits (1014), Expect = e-111, Method: Compositional matrix adjust. Identities = 232/549 (42%), Positives = 316/549 (57%), Gaps = 18/549 (3%) Query: 5 YDYVIVGAGSAGCVLANRLSRDAGYRVLLLEAGGEDSNIWLKIPAGVPRVV-GHPDLT-W 62 +DYVIVG GSAG LA RLS D V LLEAGG+ ++ ++ PA V ++ G+ L W Sbjct: 3 FDYVIVGGGSAGATLAARLSEDPRISVCLLEAGGQGDSLLVRTPAAVVAMLPGYGKLNNW 62 Query: 63 GYMSEPEPGLNNRKIIWPRGKTLGGSSSINGHVYMRGNPGDYDGWEAMGNVGWGWKDVLP 122 + P+PGLN R+ PRG+ LGGSS+IN +Y+RG DYDGW G GW W+ VLP Sbjct: 63 ALQTTPQPGLNGRRGYQPRGRALGGSSAINAMLYVRGQRQDYDGWAQAGCPGWDWESVLP 122 Query: 123 YFRRSESHFTGASDLHGADGELHVSQLAEPHDASKAFVAAAQHIGIERNDDFNGATQEGV 182 YF+R+E++ GA HGA G L VS+ P ++AF+ A Q G DFN EGV Sbjct: 123 YFKRAENNVRGADAWHGASGPLQVSEQNRPRPITRAFIEAGQARGHRLCQDFNTGDNEGV 182 Query: 183 GYLQFS-----IHKGVRDSASAAFLHPVRNRR-NLTVLTHALAESLEFEGKRVTGVRVRV 236 G Q + H+G R SA+AA+LHPV +R NL+VL A+ L FEGKR GV R Sbjct: 183 GLYQVTQFHTPAHRGERCSAAAAYLHPVMGQRPNLSVLRQVRAQRLLFEGKRAIGVAYRQ 242 Query: 237 NGNVRVVHASREVILSGGTINSPQLLMLSGIGPAEQLQAMGIPVKMNVPAVGQNLHDHIY 296 V A+REVI++ G SPQLL LSG+G ++ + GI + +P VGQNL DH+ Sbjct: 243 QQGDAQVRAAREVIIAAGAFGSPQLLQLSGVGRSDDILPHGIALHHPLPGVGQNLQDHLD 302 Query: 297 AHCLVSVDPTFSINRMISSNWRMIPDVFKYLATRRGLLTSAAAQVGLFVRTEQGLQYPDL 356 + T + + R++ + + GL+ + A+ F++T L PD+ Sbjct: 303 FTQGWTTRDTDNFGLGVVGGLRLLGQLLPWKRHGEGLIATPFAEGAAFLKTRPELDRPDI 362 Query: 357 QVQMRPFSMISKSAGMYKAEPAFCXGEGAPAVTASCTLLRPYSKGEVTLRSADATQAPRM 416 Q+ +++ A A F + +LRP+S+G V L+SAD P + Sbjct: 363 QLHF-CIAIVDDHARKLHAGYGF---------SLHMCMLRPHSRGRVGLQSADPMADPLI 412 Query: 417 VANYLTDDRDLPPLMEGLRMIRRIFAADPFRPKLRDEIMPGTAHQSDKELISYLRGNAQS 476 YL+D RDL +++G RM R+I +P R R E+ G D + S +R A + Sbjct: 413 DPGYLSDPRDLATMIDGARMARQIVMTEPLRHYCRRELFGGRDDMDDAQWESMIRHRADT 472 Query: 477 MYHPVGTCRMGVDAGAVVDPELKLNGFEGVRVVDASVMPRIPSGNTNAPTIMIAEKAADM 536 +YHP GTCRMG DA AVVD +L+++G +G+RVVDASVMP + SGNTNAPTIMIAEKAADM Sbjct: 473 IYHPAGTCRMGEDAMAVVDAQLRVHGLQGLRVVDASVMPTLVSGNTNAPTIMIAEKAADM 532 Query: 537 IIAERQLVR 545 I A + R Sbjct: 533 IRASHEQAR 541 >gi|300314074|ref|YP_003778166.1| choline dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 539 Score = 389 bits (1000), Expect = e-109, Method: Compositional matrix adjust. Identities = 225/543 (41%), Positives = 308/543 (56%), Gaps = 20/543 (3%) Query: 5 YDYVIVGAGSAGCVLANRLSRDAGYRVLLLEAGGEDSNIWLKIPAGVPRVVGHPDLTWGY 64 YDY+I+GAG+AGCV+ANRLSR G +VLL+EAG +D IW+ IP G + +P W + Sbjct: 7 YDYIIIGAGTAGCVMANRLSRKTGKKVLLIEAGAKDDYIWIHIPVGYLYCINNPRTDWMF 66 Query: 65 MSEPEPGLNNRKIIWPRGKTLGGSSSINGHVYMRGNPGDYDGW-EAMGNVGWGWKDVLPY 123 +E + GLN R +I+PRGK LGG SSING +YMRG DYD W + G+ W W++VLP Sbjct: 67 RTEADAGLNGRSLIYPRGKVLGGCSSINGMIYMRGQARDYDHWADVTGDDSWRWQNVLPL 126 Query: 124 FRRSESHFTGASDLHGADGELHVSQLAEPHDASKAFVAAAQHIGIERNDDFNGATQEGVG 183 F++SE + GA HGA GE V + D AF AA GI + +DFN EG G Sbjct: 127 FKKSEDYHLGAGQFHGAGGEWRVEKQRLRWDILDAFRDAAAENGIPKVEDFNCGDNEGCG 186 Query: 184 YLQFSIHKGVRDSASAAFLHPVRNRRNLTVLTHALAESLEFE----GKRVTGVRVRVNGN 239 Y + +GVR +AS AFL P NLT++T + L E G TGV G+ Sbjct: 187 YFDVNQKRGVRWNASKAFLRPAMKDGNLTIMTGSHVSRLRMEQGEQGPVCTGVEFTGGGS 246 Query: 240 VRVVHASREVILSGGTINSPQLLMLSGIGPAEQLQAMGIPVKMNVPAVGQNLHDHIYAHC 299 A +E IL G I SP +L +SGI LQ IPV +P VG+NL DH+ Sbjct: 247 AWFAEA-KETILCAGAIGSPHILQMSGIADPALLQQHQIPVVHALPGVGENLQDHLQMRM 305 Query: 300 LVSVDPTFSINRMISSNWRMIPDVFKYLATRRGLLTSAAAQVGLFVRTEQGLQYPDLQVQ 359 + V+ ++N M S+ + +YL T+ G ++ A +Q+G F +++ +LQ Sbjct: 306 VFKVNGAKTLNAMASTLVGKMQIGLQYLFTQSGPMSMAPSQLGAFAKSDAQQASANLQYH 365 Query: 360 MRPFSMISKSAGMYKAEPAFCXGEGAPAVTASCTLLRPYSKGEVTLRSADATQAPRMVAN 419 ++P S+ K A PAF TAS LRP S+G V L S D APR+ N Sbjct: 366 VQPLSL-EKFGDPLHAFPAF---------TASVCNLRPTSRGHVRLASGDHALAPRITTN 415 Query: 420 YLTDDRDLPPLMEGLRMIRRIFAADPFRPKLR-DEIMPGTAHQSDKELISYLRGNAQSMY 478 YL+ + DL LR+ RRI AA P + R +E PG ++++++L +++ Sbjct: 416 YLSTEEDLKVAANALRLTRRI-AASPALSRYRPEEYKPGAHYETEEDLYRAAGEVGTTIF 474 Query: 479 HPVGTCRMGV--DAGAVVDPELKLNGFEGVRVVDASVMPRIPSGNTNAPTIMIAEKAADM 536 HPVGTCRMG DA AVVD + ++ G G+ V DASVMP I SGNTN+PT+MIAEK A Sbjct: 475 HPVGTCRMGRSDDAMAVVDAQGRVRGVGGLSVADASVMPSITSGNTNSPTVMIAEKVASS 534 Query: 537 IIA 539 +++ Sbjct: 535 LLS 537 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,771,435 Number of extensions: 77799 Number of successful extensions: 217 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 208 Number of HSP's successfully gapped: 2 Length of query: 546 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 456 Effective length of database: 1,193,720 Effective search space: 544336320 Effective search space used: 544336320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)