BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0057.p (388 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313279|ref|YP_003777371.1| vanillate O-demethylase oxygena... 73 4e-14 >gi|300313279|ref|YP_003777371.1| vanillate O-demethylase oxygenase subunit oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 349 Score = 72.8 bits (177), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 55/169 (32%), Positives = 82/169 (48%), Gaps = 10/169 (5%) Query: 30 RNHWYPIKLSREIGESQVVKATVCGENILLKR-IDGKVFAIRDRCIHRGVPLSEKLECYS 88 +N WY EI +S+ + +CGE I+ R GKV A+ D C HRG PLS L Sbjct: 4 KNAWYVACTPDEI-DSKPLGRQICGEKIVFYRGAGGKVAAVEDFCPHRGAPLS--LGFVR 60 Query: 89 KGTITCWYHGFTYKWENGELCDILASPDSRAIGRRSIKTYPVEEAKGVVFVFVGDIDTTP 148 +G + C YHG E G ++ P R G I++YPVEE G ++V+ GD + Sbjct: 61 EGQLVCGYHGL----EMGCDGKAISMPGQRVRGFPCIRSYPVEERYGFIWVWPGDKEQA- 115 Query: 149 HPLSEDVPPMFLDADMALEGDSYMVDSNWRVGCENGFDGLH-VYIHRTS 196 P + + A G Y ++ ++R+ +N D H Y+H +S Sbjct: 116 DPAQIHHLEWADNPEWAYGGGLYHINCDYRLMIDNLMDLTHETYVHASS 164 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.138 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,278,727 Number of extensions: 52880 Number of successful extensions: 104 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 104 Number of HSP's successfully gapped: 1 Length of query: 388 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 301 Effective length of database: 1,207,925 Effective search space: 363585425 Effective search space used: 363585425 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)