BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0053.p (70 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311802|ref|YP_003775894.1| extradiol dioxygenase [Herbaspi... 101 1e-23 >gi|300311802|ref|YP_003775894.1| extradiol dioxygenase [Herbaspirillum seropedicae SmR1] Length = 138 Score = 101 bits (251), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 45/70 (64%), Positives = 53/70 (75%) Query: 1 MPHLGIILQMEEWRVLADTLSNSKNFKFILSPNIRFPGEPGEQATMFFQDPFGNPIEAKG 60 MPHLG+IL M +W+ LAD LS S +F+L+P IRF GEPGEQATMFF DP GNPIE KG Sbjct: 69 MPHLGVILPMPQWQALADRLSASAQVQFVLAPQIRFAGEPGEQATMFFCDPSGNPIEVKG 128 Query: 61 FTNLSGVYEK 70 F +L VY + Sbjct: 129 FADLQQVYAR 138 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.140 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 235,701 Number of extensions: 7636 Number of successful extensions: 10 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 10 Number of HSP's successfully gapped: 1 Length of query: 70 Length of database: 1,619,870 Length adjustment: 42 Effective length of query: 28 Effective length of database: 1,421,000 Effective search space: 39788000 Effective search space used: 39788000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 123 (52.0 bits)