BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0046.p (447 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 239 3e-64 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 228 7e-61 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 213 4e-56 gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 207 2e-54 gi|300313056|ref|YP_003777148.1| major facilitator superfamily p... 205 5e-54 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 239 bits (611), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 143/424 (33%), Positives = 238/424 (56%), Gaps = 13/424 (3%) Query: 26 QARATARKAAIAASAGTAIEYYEFGVYGYMAATIGPLFFPSTDATASLLAILAVFGSAFL 85 Q + ++ IAA G A+E+Y+F VYG+ +A I LFFP+ + SLL LA FG F Sbjct: 2 QPQLNKQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGFF 61 Query: 86 MRPIGGIVLGRLGDRIGRRGILLVTVIGMGIATGLIGLLPTASTAGILAPLMLLLVRLVQ 145 MRP+GG++LG DR GR+ + V ++ M +A +I PT +T GI AP+M+++ R++Q Sbjct: 62 MRPVGGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQ 121 Query: 146 GFFAGGEVTGAAAYVAECAPPGKRGFYGAFTPVGVAVGGAVAAALCGITSVLLTPAQMAE 205 GF GGE + + A++ E AP KRG YG++ VG + A + + + L+P + Sbjct: 122 GFATGGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDS 181 Query: 206 WGWRIPFLLAIPMVLISTAVRRRVEESAAFQEFQAHNEPVKAPLR--EVLTQHPGKIFKV 263 WGWR+PF+L + + + +RR + E+ F E + N+P AP++ +V+ + + Sbjct: 182 WGWRLPFVLGLLIGPVGLWIRRHMHETDDFLE--SANKPNAAPIKLLDVVKTNLRGVLVS 239 Query: 264 MALAFGQNVGYYVGLVFMNIYMTTYLKYDKTAIYWIMAAVGVTMAMLMPFFGSLSDRWGR 323 M V +YV LV M + L + + + ++PF G+LSDR GR Sbjct: 240 MGQVINGTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLGR 299 Query: 324 RPVLAIGFIGYMVLVWPM-MFLMDQQSLPLAALAMFIMALPLPIVQSVGY---PT-YAEQ 378 R VL + ++++V+P+ +++ SLP + +M + L IV + PT +EQ Sbjct: 300 RTVLLYSSLAFLLMVYPLFVWVSAAPSLP----RLLVMQVLLCIVIGANFGPMPTALSEQ 355 Query: 379 FPTKVRYTGMAFSINIGAILGGGVTPYLATSLMATTGNLKSPAFLLIAAAVLSLLALLTV 438 FPT+VR TG+A + N+ A+L GG P++ T L T+G+ +PA+ ++ AA + + A + Sbjct: 356 FPTRVRSTGLAVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGVSAAWAM 415 Query: 439 HETA 442 + + Sbjct: 416 RDQS 419 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 228 bits (581), Expect = 7e-61, Method: Compositional matrix adjust. Identities = 143/423 (33%), Positives = 226/423 (53%), Gaps = 6/423 (1%) Query: 20 ADKLDPQARATARKAAIAASAGTAIEYYEFGVYGYMAATIGPLFFPSTDATASLLAILAV 79 AD + + R+A +A G +E+++F VY + A I LFFP+ + SLL +A Sbjct: 8 ADGIVSVSNQQRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVAT 67 Query: 80 FGSAFLMRPIGGIVLGRLGDRIGRRGILLVTVIGMGIATGLIGLLPTASTAGILAPLMLL 139 FG F MRP+GGI+LG DR+GR+ L VT++ M + T +IGL PT + G+ APL+++ Sbjct: 68 FGVGFFMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIV 127 Query: 140 LVRLVQGFFAGGEVTGAAAYVAECAPPGKRGFYGAFTPVGVAVGGAVAAALCGITSVLLT 199 + RL+QGF AGGE+ A A++ E AP +R +Y ++ + V + AA+ + L Sbjct: 128 VARLLQGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLD 187 Query: 200 PAQMAEWGWRIPFLLAIPMVLISTAVRRRVEESAAFQEFQAHNEPVKAPLREVLTQHPGK 259 A +A WGWR+PFLL I + + +R +EE+ F E + +PL+EV+ P + Sbjct: 188 AAALASWGWRLPFLLGIIIGPVGYYIRHHLEETPTFLE---EKDKTDSPLKEVIRNFPRE 244 Query: 260 IFKVMALAFGQNVGYYVGLVFMNIYMTTYLKYDKTAIYWIMAAVGVTMAMLMPFFGSLSD 319 ++ V YV L +M Y LK +T + G+ + +L P G L+D Sbjct: 245 TSASFSMVILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLAD 304 Query: 320 RWGRRPVLAIGFIGYMVLVWPMMFLMDQQSLPLAALAMFIMALPLPIVQSVG--YPTYAE 377 R GRRP+L+ ++L +P MF + L+ L +F + I G ++E Sbjct: 305 RIGRRPLLSGSAALILMLAYP-MFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSE 363 Query: 378 QFPTKVRYTGMAFSINIGAILGGGVTPYLATSLMATTGNLKSPAFLLIAAAVLSLLALLT 437 FP KV TG++ + N+ + GG + T L+A TG+ +PAF ++ AA +S + Sbjct: 364 LFPAKVLSTGLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISFVGTRF 423 Query: 438 VHE 440 V E Sbjct: 424 VRE 426 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 213 bits (541), Expect = 4e-56, Method: Compositional matrix adjust. Identities = 140/437 (32%), Positives = 228/437 (52%), Gaps = 29/437 (6%) Query: 11 SSPAAEAGIADKLDPQARATARKAAIAASAGTAIEYYEFGVYGYMAATIGPLFFPSTDAT 70 SS A G AD PQAR +A I++S G A+E+++ +YG A I FFP+ D + Sbjct: 2 SSNTAAPG-ADITHPQAR----RAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDS 56 Query: 71 ASLLAILAVFGSAFLMRPIGGIVLGRLGDRIGRRGILLVTVIGMGIATGLIGLLPTASTA 130 SLL A FG +F MRP+G +VLG DR GR+ L+++++ M + T +I +P+ ++ Sbjct: 57 VSLLLTFATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASI 116 Query: 131 GILAPLMLLLVRLVQGFFAGGEVTGAAAYVAECAPPGKRGFYGAFTPVGVAVGGAVAAAL 190 G+LAP + L +++QGF AGGE + A++ E A P +RGF+ ++ + +AA Sbjct: 117 GLLAPAGIALGKMIQGFSAGGEFGSSTAFLVEHA-PHRRGFFSSWQVASQGISLLLAALF 175 Query: 191 CGITSVLLTPAQMAEWGWRIPFLLAIPMVLISTAVRRRVEESAAFQEFQAHNEPVKAPLR 250 + + +LTP Q+A WGWR+PF+ + + +RR ++E+ EF+ +E APLR Sbjct: 176 GAVLNNMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEA---PEFKESSEKTNAPLR 232 Query: 251 EVLTQHPGKIFKVMALAFGQNVGYYVGLVFMNIYMTTYLKYDKTAIYWIMAAVGVTMAML 310 + ++ V Y+ L ++ Y L + + M+ G+ M + Sbjct: 233 DTFAHQKMRLLIGAGSVIMATVSVYLSL-YIPTYAVKQLGLPAWSSFAAMSVAGLIMFIG 291 Query: 311 MPFFGSLSDRWGRRPVLAIGFIGYMVLVWPM-MFLMDQ---------QSLPLAALAMFIM 360 P G+LSD+ GR P + Y+VL +PM +FL + Q++ + M+ Sbjct: 292 SPLVGALSDKIGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFA 351 Query: 361 ALPLPIVQSVGYPTYAEQFPTKVRYTGMAFSINIGAILGGGVTPYLATSLMATTGNLKSP 420 A+P A+ FP R TGM+ + NI + GG + T L+ TG+ S Sbjct: 352 AMP---------ALLADIFPVATRGTGMSLAYNIAVTIFGGFAGLIITWLIDFTGDKLSV 402 Query: 421 AFLLIAAAVLSLLALLT 437 ++ +I AVLSL+A L+ Sbjct: 403 SYFVIFGAVLSLIASLS 419 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 207 bits (526), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 136/418 (32%), Positives = 220/418 (52%), Gaps = 19/418 (4%) Query: 39 SAGTAIEYYEFGVYGYMAATIGPLFFPSTDATASLLAILAVFGSAFLMRPIGGIVLGRLG 98 ++G +E ++F ++G+ A I FFPS + ASL+ FG+ FLMRP+G I+LG Sbjct: 18 TSGNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTFGAGFLMRPLGAIILGAYV 77 Query: 99 DRIGRRGILLVTVIGMGIATGLIGLLPTASTAGILAPLMLLLVRLVQGFFAGGEVTGAAA 158 DR+GRR L+VT+ M + T LI +P+ +T G LAPL++L+ RL+QGF AG E+ G + Sbjct: 78 DRVGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLIGRLLQGFSAGVELGGVSV 137 Query: 159 YVAECAPPGKRGFYGAFTPVGVAVGGAVAAALCGITSVLLTPAQMAEWGWRIPFLLAIPM 218 Y+AE A PG +GFY ++ V VAAA+ + S LTPA+++ WGWRIPF + + Sbjct: 138 YLAEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTPAEVSGWGWRIPFFIGCLI 197 Query: 219 VLISTAVRRRVEESAAFQEFQAHNEPVKAPLREVLTQHPGKIFKVMALAFGQNVGYYVGL 278 V + +RR ++E+ F + + P + + ++ + M L V +Y+ Sbjct: 198 VPVLFVIRRSLQETEEFM--RRKHRPSTGQIFRSMIENWKLVIAGMMLVSMTTVSFYLIT 255 Query: 279 VFMNIYMTTYLKYDKTAIYWIMAAVGVTMAMLMPFFGSLSDRWGRRPVLAIGFIGYMVLV 338 V+ + LK A + VG++ + +P G+LSDR GRRP+L + ++ Sbjct: 256 VYTPTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDRVGRRPLLVTFTVLTILTA 315 Query: 339 WP-MMFLMDQQSLPLAALAMFIMALPLPIVQSVGYPTY--------AEQFPTKVRYTGMA 389 +P + +L+ S M I+ L L + Y +Y E P VR G + Sbjct: 316 YPAVNWLVHDASFA----KMLIVELWLSFL----YASYNGAMVVALTEVMPADVRTAGFS 367 Query: 390 FSINIGAILGGGVTPYLATSLMATTGNLKSPAFLLIAAAVLSLLALLTVHETAKEKLA 447 + ++ + GG TP +AT L+ T + +P + AAV L+A L ++ + A Sbjct: 368 LAYSLATAVFGGFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIATLILYRNKAVRQA 425 >gi|300313056|ref|YP_003777148.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 465 Score = 205 bits (522), Expect = 5e-54, Method: Compositional matrix adjust. Identities = 139/435 (31%), Positives = 232/435 (53%), Gaps = 30/435 (6%) Query: 31 ARKAAIAASAGTAIEYYEFGVYGYMAATIGP-LFFPSTDATASLLAILAVFGSAFLMRPI 89 ++KAA + G+A+EYY+F +Y A+ I P LFFP D T +++A LA +G ++ RPI Sbjct: 13 SKKAAASGWIGSALEYYDFFIYATAASLIFPQLFFPKGDPTTAIIASLATYGVGYVARPI 72 Query: 90 GGIVLGRLGDRIGRRGILLVTVIGMGIATGLIGLLPTASTAGILAPLMLLLVRLVQGFFA 149 G VLG LGD GR+ +LL + MG +T +GLLPT G+ AP +L+++RL QGF Sbjct: 73 GAFVLGHLGDTHGRKKVLLWCMFLMGFSTIGVGLLPTYDQVGLWAPALLVIMRLAQGFAV 132 Query: 150 GGEVTGAAAYVAECAPPGKRGFYGAFTPVGVAVGGAVAAALCGITSVLLTPAQMAEWGWR 209 GE++GA++ + E +P G+RG++ +FT GV G +AAA+ + + Q WGWR Sbjct: 133 AGEISGASSMILEHSPFGRRGYFSSFTLQGVQAGQILAAAVFLPLAHYMPAEQFNVWGWR 192 Query: 210 IPFLLAIPMVLISTAVRRRVEESAAFQEFQAHNEPVKAPLREVLTQHPGKIFKVMALAFG 269 IPFLL+ +++ +RR V+E+ F+ ++P+ + + +V+ +A Sbjct: 193 IPFLLSFLVIIAGWVIRRTVDETPVFKAADKEELKRRSPVVDAFRDCSPNMLRVVCMAL- 251 Query: 270 QNVGYYVGLVFMNIYMTTY---LKYDKTAIYWIMAAVGVTMAMLMPFFGSLSDRWGRRPV 326 NV V +F Y + + K WI + +++P+ G LSDR GRRP Sbjct: 252 MNVIPVVATIFGAAYAVQPAYGIGFAKDVYLWIPVVGNIVAVLVIPYVGKLSDRIGRRPP 311 Query: 327 LAIGFIGYMVLVWPMMFLMDQQSLPLAALAMFIMALPLPIVQSVGY----PT-YAEQFPT 381 + +G + + + ++ + +++ LA F+M++ + V GY P+ Y E FP Sbjct: 312 IIVGSLLAGLFAFAYLYAISIRNVELA----FVMSILMWGVVYQGYNAVFPSFYPELFPA 367 Query: 382 KVRYTGMAFSINIGAILGG-------GVTPYLATSLMATTGNLKSPAFLLIAAAVLSLLA 434 + R + MA + N+G + V P ++++ T G++ A ++S LA Sbjct: 368 RARVSAMAIAQNLGTAITAMLPALFTAVAPPGSSNIWLTVGSIA------FAITIVSALA 421 Query: 435 LLTVHETAK---EKL 446 L+ ET + EKL Sbjct: 422 ALSARETYRIPLEKL 436 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.138 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,112,571 Number of extensions: 41462 Number of successful extensions: 356 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 335 Number of HSP's successfully gapped: 16 Length of query: 447 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 359 Effective length of database: 1,203,190 Effective search space: 431945210 Effective search space used: 431945210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)