BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0044.p (393 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoac... 641 0.0 gi|300310161|ref|YP_003774253.1| aminohydrolase [Herbaspirillum ... 386 e-108 gi|300310472|ref|YP_003774564.1| hippurate hydrolase [Herbaspiri... 345 5e-96 >gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoacylase/carboxypeptidase [Herbaspirillum seropedicae SmR1] Length = 399 Score = 641 bits (1653), Expect = 0.0, Method: Compositional matrix adjust. Identities = 299/389 (76%), Positives = 345/389 (88%) Query: 5 FCRIADLDDVKDELVGIRRHIHQHPELSYEEVDTAALVAARLESWGYAVARNIGGHGLVA 64 C + DL D++DE+ IRRH+HQHPELS+EEVDTAALVA RLE WGYAV R+IGG+GLVA Sbjct: 9 LCEVGDLHDIQDEITAIRRHLHQHPELSFEEVDTAALVAQRLEQWGYAVTRHIGGNGLVA 68 Query: 65 TLRSGSSTRSIGVRADMDALPINEMTGLSYASERQGKMHACGHDGHTAMLLGAALQLSRT 124 TLR GSS RSIG+RADMDALPI E TGL +AS + G MHACGHDGHTAMLLGAA L+RT Sbjct: 69 TLRVGSSARSIGLRADMDALPIQEETGLDWASVKPGAMHACGHDGHTAMLLGAARHLART 128 Query: 125 RNFDGTVNLIFQPAEEAGFNSGAVQMIRDGLFERFPCDAIFGMHNHPGVETGTFMFRAGP 184 R FDGT+NLIFQPAEEAGF+SGA +M+ DGLFERFPC+A+FG+HNHPGVE GTFMFR+GP Sbjct: 129 RRFDGTLNLIFQPAEEAGFDSGAQKMLADGLFERFPCEAVFGIHNHPGVEAGTFMFRSGP 188 Query: 185 FMAACDTVKIKILGKGSHAARPHQSIDPLVAASSLVMALQTVVSRNIDPMEAAVVTVGSL 244 FMAACDTVKI+I G+GSHAARPH S+DP+V A+SLVMALQTVVSRNIDPM++AVVTVGSL Sbjct: 189 FMAACDTVKIRITGRGSHAARPHLSVDPVVVAASLVMALQTVVSRNIDPMDSAVVTVGSL 248 Query: 245 HAGHAANVIPEHATMELSVRSFKQEVRELLEQRIRALVQSHVEGYGAKAEVEYIRGYPVL 304 HAG A+NVIPE ATMELSVRSFK EVRELLEQRIRALV +H + YGA+AE++Y+RGYPVL Sbjct: 249 HAGKASNVIPEFATMELSVRSFKPEVRELLEQRIRALVSTHAQSYGAQAEIDYLRGYPVL 308 Query: 305 VNSEAETEFAKQIAVELVGADKIIPSFPPIAGSEDFAYFLQQRPGCFLRVGNGAGKPMLH 364 VNS+AET+FA+ +A ELVG +K+I F PIAGSEDFAYFL+QRPGCFLRVGNG GKPMLH Sbjct: 309 VNSDAETDFARSVAEELVGPEKVIAPFGPIAGSEDFAYFLRQRPGCFLRVGNGQGKPMLH 368 Query: 365 NAHYDFSDDNVTVGAAYWTRLVERYLQAQ 393 NA YDF+D N+ +GAAYWTRLVER++ Q Sbjct: 369 NAGYDFNDANIPIGAAYWTRLVERWMPLQ 397 >gi|300310161|ref|YP_003774253.1| aminohydrolase [Herbaspirillum seropedicae SmR1] Length = 402 Score = 386 bits (992), Expect = e-108, Method: Compositional matrix adjust. Identities = 194/385 (50%), Positives = 258/385 (67%), Gaps = 13/385 (3%) Query: 17 ELVGIRRHIHQHPELSYEEVDTAALVAARLESWGYAVARNIGGHGLVATLRSGSSTRSIG 76 +LV IRRH+H HPEL +EE TA+ VA L S+G VA NIGG+G+VATLR G STR+I Sbjct: 16 DLVSIRRHLHAHPELRFEEKRTASFVADMLRSYGLTVAENIGGYGVVATLRKGKSTRAIA 75 Query: 77 VRADMDALPINEMTGLSYASERQGKMHACGHDGHTAMLLGAALQLSRTRNFDGTVNLIFQ 136 +RADMDALP++E S+ S GKMHACGHDGHT MLLGAA +LSR FDGTV+ +FQ Sbjct: 76 LRADMDALPMSEQNDFSHISTCAGKMHACGHDGHTTMLLGAARRLSREVEFDGTVHFVFQ 135 Query: 137 PAEEAGFNSGAVQMIRDGLFERFPCDAIFGMHNHPGVETGTFMFRAGPFMAACDTVKIKI 196 PAEE G +GA MI DGLFERFP DAIFG+HN PG+ G+F R GP MA+ +T + Sbjct: 136 PAEEGG--AGARLMIEDGLFERFPADAIFGVHNWPGLPAGSFGLRPGPLMASSNTFSATL 193 Query: 197 LGKGSHAARPHQSIDPLVAASSLVMALQTVVSRNIDPMEAAVVTVGSLHAGHAANVIPEH 256 G+G+H A+PH+SIDP++AA+ L +A Q++V+RNI+P AV++V LH G A NVIPE Sbjct: 194 FGRGAHGAQPHRSIDPVLAAAQLTLAWQSIVTRNINPNHRAVISVTQLHTGTADNVIPEQ 253 Query: 257 ATMELSVRSFKQEVRELLEQRIRALVQSHVEGYGAKAEVEYIRGYPVLVNSEAETEFAKQ 316 AT+ +VRSF E +L+E R+ A+ ++ + + + + + R YP +VN T+ A Q Sbjct: 254 ATLSGTVRSFDAETLDLIEHRMAAIAEAIGQMFDLRVQFHFERLYPAVVNHPEATQQAMQ 313 Query: 317 IAVELVGADKIIPSFPPIAGSEDFAYFLQQRPGCFLRVGNG-----------AGKPMLHN 365 + VGA+++ P SEDFA++LQ +PGC+ +GNG A LH+ Sbjct: 314 AMMAAVGAEQVDGDTEPAFASEDFAFYLQHKPGCYAFLGNGVIADGKQAGSQATSRELHS 373 Query: 366 AHYDFSDDNVTVGAAYWTRLVERYL 390 YDF+DD + G AYW LV +YL Sbjct: 374 PFYDFNDDIIDAGVAYWVSLVRQYL 398 >gi|300310472|ref|YP_003774564.1| hippurate hydrolase [Herbaspirillum seropedicae SmR1] Length = 403 Score = 345 bits (884), Expect = 5e-96, Method: Compositional matrix adjust. Identities = 169/374 (45%), Positives = 237/374 (63%), Gaps = 5/374 (1%) Query: 20 GIRRHIHQHPELSYEEVDTAALVAARLESWGYAVARNIGGHGLVATLRSGSSTRSIGVRA 79 G+RR +H HPEL +EE TA +V L+S GY V R +GG G+VA+L R I +RA Sbjct: 20 GLRRDLHAHPELRFEEHRTADVVVRELQSLGYTVTRGLGGTGVVASLAGADPQRGIVLRA 79 Query: 80 DMDALPINEMTGLSYASERQGKMHACGHDGHTAMLLGAALQLSRTRNFDGTVNLIFQPAE 139 D+DALPI E ++AS G MHACGHDGHT MLLGAA L + G+V+ +FQP E Sbjct: 80 DLDALPIVEANDFAHASCTHGVMHACGHDGHTVMLLGAARLLKQGPMLPGSVHFVFQPGE 139 Query: 140 EAGFNSGAVQMIRDGLFERFPCDAIFGMHNHPGVETGTFMFRAGPFMAACDTVKIKILGK 199 E G +GA +MI DGLFE+FP +A+FGMHN PG+ G F R GP MAA +KI I GK Sbjct: 140 EGG--AGARKMIDDGLFEQFPTEAVFGMHNWPGLPAGQFGLRPGPIMAAGSRLKITITGK 197 Query: 200 GSHAARPHQSIDPLVAASSLVMALQTVVSRNIDPMEAAVVTVGSLHAGHAANVIPEHATM 259 G+HAA+PH +DP+ A S+V+ QT+ +R+ DP++ AV++V HAG NVIP+ A + Sbjct: 198 GAHAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVISVCMFHAGDTDNVIPDRAEL 257 Query: 260 ELSVRSFKQEVRELLEQRIRALVQSHVEGYGAKAEVEYIRGYPVLVNSEAETEFAKQIAV 319 ++R+ +++ L++ IR + + E YGAK EVE+ + YP +N+ AET+F +++ Sbjct: 258 RGTIRTLSSTLQQKLQEDIRRMCHALAEAYGAKVEVEFFQYYPATINTPAETDFCERVIR 317 Query: 320 ELVGADKIIPSFPPIAGSEDFAYFLQQRPGCFLRVGNGA---GKPMLHNAHYDFSDDNVT 376 + G D+I P SEDF + L++RPG ++ +GN LH+ HYDF+DD + Sbjct: 318 QTFGDDRIRTGIPANMTSEDFGFMLEERPGAYVLIGNAPQDRASHSLHHPHYDFNDDIIE 377 Query: 377 VGAAYWTRLVERYL 390 G YW L + Y Sbjct: 378 AGVRYWVALAQGYF 391 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,253,771 Number of extensions: 53860 Number of successful extensions: 153 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 149 Number of HSP's successfully gapped: 3 Length of query: 393 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 306 Effective length of database: 1,207,925 Effective search space: 369625050 Effective search space used: 369625050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)