BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0032.p (294 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309777|ref|YP_003773869.1| transcription regulator protein... 402 e-113 gi|300313769|ref|YP_003777861.1| transcription regulator protein... 137 1e-33 gi|300309921|ref|YP_003774013.1| LysR family transcription regul... 130 2e-31 gi|300312196|ref|YP_003776288.1| LysR family transcription regul... 126 2e-30 gi|300311233|ref|YP_003775325.1| LysR family transcription regul... 109 3e-25 >gi|300309777|ref|YP_003773869.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 291 Score = 402 bits (1032), Expect = e-113, Method: Compositional matrix adjust. Identities = 193/291 (66%), Positives = 237/291 (81%) Query: 1 MDFNALEDFQLVAAHGGFGKASRASGRSKATLSRRVADLEEALGIRLIERGARSLELTEA 60 MD NAL DFQLVAAHG FG+ASR SGR+KATLSRRVA+LEE LG+RL+ERG + L +TEA Sbjct: 1 MDINALVDFQLVAAHGSFGRASRESGRAKATLSRRVAELEEELGVRLVERGTQRLTVTEA 60 Query: 61 GQLLRARTEGPMHEVADAVAAARDGLATPRGRLRIAAPLLFSQLALGRIAARFLASYPEV 120 G+LL R+EGP+ E+A+A+ AR+G + PRGRLRIAAPL+FSQLALGR+AA F A+YP+V Sbjct: 61 GELLLQRSEGPLRELAEAMTQAREGSSAPRGRLRIAAPLVFSQLALGRLAASFHAAYPQV 120 Query: 121 QIEAVAEDRLVNLVDEHFDVAIRINPSKDSALVGRCFAKDRLVLAAAPSIQMPKGGRDKP 180 +++ VAEDRLV+LV EHFD+ IRINPS D LVGRCFA+D L L AAP++ MP+ K Sbjct: 121 EVDVVAEDRLVDLVQEHFDLCIRINPSDDGNLVGRCFARDSLALVAAPTLPMPRARAGKS 180 Query: 181 FPVPAVVTPGYRDGDVWSVRNGQFTIEPRPVLRLSSLLMVRDAIVDGAGAAMLPQSIIGG 240 PVPAVV P +RD D+WS+R G+ I P+PVLRLSSL+MV+DA+ GAGAA+LP S+ Sbjct: 181 HPVPAVVMPTFRDSDLWSLREGKLLIAPQPVLRLSSLMMVKDAVCAGAGAALLPWSLASA 240 Query: 241 LLEKGELVSWGIAGDEVELWVLHTSRRLQSPKVRAFVEFMCKQYPNGTFTI 291 L+E G+L WG G EVELWVLHTSRRLQSPKVRAFV+FMC+QYP+G F + Sbjct: 241 LVEAGQLKLWGKLGGEVELWVLHTSRRLQSPKVRAFVDFMCRQYPDGRFVV 291 >gi|300313769|ref|YP_003777861.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 318 Score = 137 bits (345), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 97/297 (32%), Positives = 150/297 (50%), Gaps = 24/297 (8%) Query: 2 DFNALEDFQLVAAHGGFGKASRASGRSKATLSRRVADLEEALGIRLIERGARSLELTEAG 61 D N L F V HG F A+RA K+ +SRRVA+LE+ LG+RL++R R + +TE G Sbjct: 3 DLNDLALFAAVVVHGSFSAAARALNTPKSRVSRRVAELEQRLGVRLLQRSTRVVRVTEVG 62 Query: 62 QLLRARTEGPMHEVADAVAAARDGLATPRGRLRIAAPLLFSQLALGRIAARFLASYPEVQ 121 E + AV A P GRLR+++P+ + + L + ARFL ++P+V+ Sbjct: 63 SAFFTHCEAMTNAARAAVEVTEHAGARPAGRLRVSSPMGVAHVFLAPLLARFLCAHPDVR 122 Query: 122 IEAVAEDRLVNLVDEHFDVAIRINPS-KDSALVGRCFAKDRLVLAAAP----------SI 170 +E +R V+++ E FDVA+R+ + +DS+LV R F + VLAA+P ++ Sbjct: 123 LELELSNRRVDVIGEGFDVAMRVRSTLEDSSLVVRTFGTSQQVLAASPAYVRAHGPFDTV 182 Query: 171 QMPKGGRDKPFPVPAVVTPGYR----DGDVWSVRNGQFTIEPRPVLRLSSLLMVRDAIVD 226 Q +G R +R DG+V I+ P + + +V + Sbjct: 183 QSLQGQRGLGPGGLPGQAQRWRLQGPDGEVAE-------IDYAPAMVTDDMQLVMQTAIG 235 Query: 227 GAGAAMLPQSIIGGLLEKGELVSW--GIAGDEVELWVLHTSRRLQSPKVRAFVEFMC 281 G G A+LP ++ G + +GELV G +L + SRR P VRAF+EF+ Sbjct: 236 GVGLALLPFNVAHGAIARGELVVVLPQYRGAPHQLHAVFPSRRGLVPAVRAFIEFLA 292 >gi|300309921|ref|YP_003774013.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 299 Score = 130 bits (326), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 103/293 (35%), Positives = 146/293 (49%), Gaps = 21/293 (7%) Query: 6 LEDFQLVAAHGGFGKASRASGRSKATLSRRVADLEEALGIRLIERGARSLELTEAGQLLR 65 +E F VA G F KA+ A G SKA +SR V +LE LG RL++R R L LTEAG L Sbjct: 7 IECFIAVADLGSFVKAAEALGISKAAVSRTVLELEARLGSRLMQRTTRRLSLTEAGALYL 66 Query: 66 ARTEGPMHEVADAVAAARDGLATPRGRLRIAAPLLFSQLALGRIAARFLASYPEVQIEAV 125 R + + + +A G A P G LRI APL F L L + FL +PE+ ++ Sbjct: 67 ERCKQIVAALEEADDLLSSGSAKPTGLLRINAPLTFGVLHLAPLWPMFLERHPEISLDIT 126 Query: 126 AEDRLVNLVDEHFDVAIRINPSKDSALVGRCFAKDRLVLAAAPSIQMPKGGRDKPFPVPA 185 DR+V+++DE +D+AIRI DS LV R L + A+P+ G P + Sbjct: 127 LSDRIVDIIDEGYDMAIRIARLSDSTLVHRKLTSTSLHVCASPAYLDAHGTPAHPHELAQ 186 Query: 186 VVTPGY---RDGDVWSVRNGQFTIEPRPV-LRLSSLLMVRD------AIVDGAGAAMLPQ 235 T Y + D W QF PV +R+SS L V + A + G G P Sbjct: 187 HQTISYSYNQSKDEW-----QFNGPEGPVSVRVSSRLHVNNGDSCVQAALGGIGITRQPT 241 Query: 236 SIIGGLLEKGELVSWGIAGD----EVELWVLHTSRRLQSPKVRAFVEFMCKQY 284 +I L G+LVS I GD E+ ++ ++ SR K+RA ++F+ + + Sbjct: 242 FMIDQYLRSGQLVS--ILGDYGAPELGIYAVYPSRAHLPAKMRAMLDFLTEAF 292 >gi|300312196|ref|YP_003776288.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 126 bits (317), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 91/300 (30%), Positives = 147/300 (49%), Gaps = 22/300 (7%) Query: 1 MD-FNALEDFQLVAAHGGFGKASRASGRSKATLSRRVADLEEALGIRLIERGARSLELTE 59 MD A++ F V G F A +G+SK +SR+V++LE+ LGIRL+ R R L LT+ Sbjct: 1 MDKLQAMQTFVAVVESGSFVAAVDVTGQSKPAVSRQVSELEQFLGIRLLHRTTRRLSLTD 60 Query: 60 AGQLLRARTEGPMHEVADAVAAARDGLATPRGRLRIAAPLLFSQLALGRIAARFLASYPE 119 G++ AR + + + D + GRLRI AP F L + +F +P Sbjct: 61 EGRVYFARCKELLETIQDVESEVGLNSDQACGRLRIGAPQDFGVEQLAPLWGKFTEQHPL 120 Query: 120 VQIEAVAEDRLVNLVDEHFDVAIRINPSKDSALVGRCFAKDRLVLAAAPSIQMPKGG--- 176 + + DR ++L++E FD+AIRI+ DS +V R A R++ AAP G Sbjct: 121 ITLNITLSDRTIDLLEEGFDMAIRISALGDSRMVARPLAPVRMIACAAPEFLARHGTPGT 180 Query: 177 ----RDKPFPVPAVVTPGYR-DGDVWSVRN---GQFTIEPRPVLRLSSLLMVRDAIVDGA 228 RD PF ++ YR GD WS R+ + R + +++ R + G Sbjct: 181 PAELRDYPF-----ISYSYRAGGDDWSFRHVHGDTCQVRVRSTVYVNNGATCRAMAIAGQ 235 Query: 229 GAAMLPQSIIGGLLEKGELVS----WGIAGDEVELWVLHTSRRLQSPKVRAFVEFMCKQY 284 G A+ P I+ ++ G+LV W G+++ + L+ +R PKV+ +F+ +Q+ Sbjct: 236 GIAIQPDFIVNAAIQAGQLVELFEGWS-TGEQLVVHTLYPTRTHLPPKVQRMRDFLIEQF 294 >gi|300311233|ref|YP_003775325.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 315 Score = 109 bits (272), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 88/292 (30%), Positives = 136/292 (46%), Gaps = 12/292 (4%) Query: 3 FNALEDFQLVAAHGGFGKASRASGRSKATLSRRVADLEEALGIRLIERGARSLELTEAGQ 62 N + F V G F +A+R G + + +SR +A LE+ALG +L++R R L L+E+GQ Sbjct: 10 LNEMAVFAKVVETGSFSEAARQLGATPSAISRAIARLEKALGTQLLQRTTRKLRLSESGQ 69 Query: 63 LLRARTEGPMHEVADAVAAARDGL-ATPRGRLRIAAPLLFSQLALGRIAARFLASYPEVQ 121 + AR M A AV A A P+G++R++ P + FLA++P+V Sbjct: 70 DIHARCRD-MLNAAQAVLAVSGAFDAEPQGQVRVSVPKAVGHALVHPHVPDFLAAHPKVD 128 Query: 122 IEAVAEDRLVNLVDEHFDVAIRINPSKDSALVGRCFAKDRLVLAAAPSIQMPKGGRDKP- 180 + EDR ++L+D+ D+AIRI L+GR + +L A P G P Sbjct: 129 VLLRLEDRYMDLIDDEVDLAIRITDHPPPGLMGRRLMRIDHLLCATPHYLATHGTPAHPQ 188 Query: 181 -FPVPAVVTPGYRDGDV-WSVR--NGQFTIEPRPVLRLSSLLMVRDAIVDGAGAAMLPQS 236 + + G D W R +E R + + DA++ G A LP Sbjct: 189 ALKEHSCIYLGESPADARWRFRRQGKSVNVEVRGRYAANHTGVRLDAVLRHLGIASLPYF 248 Query: 237 IIGGLLEKGELV----SWGIAGDEV-ELWVLHTSRRLQSPKVRAFVEFMCKQ 283 I LE+G LV W ELW L+ + R P++ AFV+F+ ++ Sbjct: 249 IARPALEEGSLVQVLPQWSFRTHYCGELWALYPATRHLPPRLGAFVQFLAQR 300 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 952,124 Number of extensions: 41624 Number of successful extensions: 302 Number of sequences better than 1.0e-08: 47 Number of HSP's gapped: 277 Number of HSP's successfully gapped: 47 Length of query: 294 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 210 Effective length of database: 1,222,130 Effective search space: 256647300 Effective search space used: 256647300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)