BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0031.p (309 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [H... 127 1e-30 gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [H... 123 2e-29 gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [H... 115 4e-27 gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [H... 100 3e-22 gi|300310417|ref|YP_003774509.1| zinc-containing alcohol dehydro... 73 3e-14 >gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 127 bits (319), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 104/324 (32%), Positives = 149/324 (45%), Gaps = 37/324 (11%) Query: 3 NIRALVLTEYGGPSALKISSIEAPVAGAGQVLVRVQAAGVNGLDWKVREGYV-------- 54 ++A+ +GGP L+ P G+VLVRV A G+N DW +REGY Sbjct: 5 TMKAIQQHAFGGPEVLRYEDAPVPELQPGEVLVRVHAVGLNPPDWYLREGYKMLPPEWQP 64 Query: 55 RDAFPLQLPTVPGIELAGIVEAAGAGTR-LRKGDRVM-------GPRGGLGAYADLVAVD 106 AFPL L G +++G+VEA + GD V G GG AYA V+V Sbjct: 65 TVAFPLIL----GTDISGVVEAVADDVKDFAVGDEVYSMVRFPSGLAGGSRAYAQYVSVA 120 Query: 107 EANLCLIPESLSFVEAAALPVASVAAWQSL-----NFAGPIQ---------AGQRILIHG 152 + + L P+ +S +AAA P++ + AWQ + + P+Q + ++++G Sbjct: 121 ASEIALKPQRISHAQAAAAPMSLLTAWQFMIELGHDIQNPLQPHRHEPVPLKDKTVVVNG 180 Query: 153 AAGGLGGFAVQYAHQAGAVVFAAALEEHAGYLRSLGADHVIAYDKEQFEASARDIDLVLD 212 AAGG+G FA+Q A GA V A H +LR LGAD I Y + E D+DLV+D Sbjct: 181 AAGGVGHFALQLAKWKGARVIAIGSGGHEAFLRDLGADEFIDYTRSAAEDVLSDVDLVID 240 Query: 213 YVGGEVLNRSWSVLAEDGVIVSTASPAIQASTPAGRRGLWFMNT---SDGARLQAIAEDV 269 VGG R L G + + A R G+ T S GA+LQ +A + Sbjct: 241 AVGGPASGRFLRTLKRGGALFPIFPLGFSGAEEAARLGVNVSTTQVRSSGAQLQQLAGLL 300 Query: 270 AHGRLHSKIGAVVRFDELPAAIER 293 G + I + + A ER Sbjct: 301 DSGVVRVAIDSTFALQDAGKAHER 324 >gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 309 Score = 123 bits (309), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 93/294 (31%), Positives = 146/294 (49%), Gaps = 5/294 (1%) Query: 4 IRALVLTEYGGPSALKISSIEAPVAGAGQVLVRVQAAGVNGLDWKVREGYVRDAFPLQLP 63 +RA+ +YG PS L + ++ G V V +QAAGV +D K+R G ++ L LP Sbjct: 1 MRAICYHQYGPPSVLHLCDLDEQPLPPGHVRVAMQAAGVAPVDTKLRAGRLQHLVALDLP 60 Query: 64 TVPGIELAGIVEAAGAGTR-LRKGDRV--MGPRGGLGAYADLVAVDEANLCLIPESLSFV 120 VPG + AG++ A G G L+ G+ V M G G + + + + P+ LS Sbjct: 61 KVPGRDGAGVITALGEGVHTLQVGEAVCVMPSMAGRGTTLAELVLPQEQVVRKPDVLSMQ 120 Query: 121 EAAALPVASVAAWQSLNFAGPIQAGQRILIHGAAGGLGGFAVQYAHQAGAVVFAAALEEH 180 +AAAL + +AW +L PI AG ++ IH +G +G VQ A GA V A+ + Sbjct: 121 QAAALLQPACSAWIALMQTAPIAAGMKVFIHAGSGAVGSLMVQLAAHLGAEVTASCRSSN 180 Query: 181 AGYLRSLGADHVIAYDKEQFEASARDIDLVLDYVGGEVLNRSWSVLAEDGVIV-STASPA 239 Y+ +LGA V+AYD+++ RD D+V+D VGG ++S+ +L G +V A+P Sbjct: 181 VEYVAALGASRVLAYDRDEL-GRVRDQDVVIDLVGGAAHDQSYRMLRRGGQLVWLVAAPI 239 Query: 240 IQASTPAGRRGLWFMNTSDGARLQAIAEDVAHGRLHSKIGAVVRFDELPAAIER 293 + G + D + LQ +A G + V+ E+ A +R Sbjct: 240 RERGEEFGVSVRRALIVEDQSILQQVAALAQQGVWRPAVSGVLPMQEIARAHQR 293 >gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 115 bits (288), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 86/242 (35%), Positives = 120/242 (49%), Gaps = 11/242 (4%) Query: 4 IRALVLTEYGGPSALKISSIEAPVAGAGQVLVRVQAAGVNGLDWKVREGYVRDAFPLQLP 63 +RA+ + E+GGP L+ P AG+VL++V AAGVN D R G P Sbjct: 1 MRAIEIKEFGGPEVLQACERPVPELKAGEVLIKVHAAGVNRPDVFQRTGNY--PVPPGAS 58 Query: 64 TVPGIELAGIVEAAGAG-TRLRKGDRVMGPRGGLGAYADLVAVDEANLCLIPESLSFVEA 122 +PG+E+AG + A G + + GD V G G YA+ A A +P+ LS +EA Sbjct: 59 DLPGLEVAGEIVAGDLGDSGFKLGDLVCALVQG-GGYAEFCAAPLAQCLPVPKGLSALEA 117 Query: 123 AALPVASVAAWQSLNFAGPIQAGQRILIHGAAGGLGGFAVQYAHQAGAVVFAAA-LEEHA 181 AALP W ++ G + + +L+ G + G+G A+Q A G VFA A E Sbjct: 118 AALPENYFTVWSNVFDRGQLSGEETLLVQGGSSGIGVTAIQIARALGHRVFATAGSAEKC 177 Query: 182 GYLRSLGADHVIAYDKEQFEA------SARDIDLVLDYVGGEVLNRSWSVLAEDGVIVST 235 LGA+ I Y E FEA R +D++LD VGG+ L R LA+DG +V Sbjct: 178 RACEELGAERAINYKTEDFEAVVKELTGGRGVDVILDMVGGDYLPREIKALADDGRLVFI 237 Query: 236 AS 237 A Sbjct: 238 AQ 239 >gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 324 Score = 99.8 bits (247), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 93/297 (31%), Positives = 134/297 (45%), Gaps = 31/297 (10%) Query: 5 RALVLTEYGGPSALKISSIEAPVAGAGQVLVRVQAAGVNGLDWKVREGYVRDAFPLQLPT 64 +A+ +++ GGP ++ +E GAG+V +R A G+N +D R G +P LP Sbjct: 3 KAIRMSKTGGPEVMEYVDVEVGEPGAGEVRIRHAAVGLNFIDCYFRAGL----YPQPLPA 58 Query: 65 VPGIELAGIVEAAGAGTR-LRKGDRVMGPRGGLGAYADLVAVDEANLCLIPESLSFVEAA 123 G E AG +EA GAG + GDRV GAYA+ + L +P+++SF AA Sbjct: 59 GLGQEAAGTIEAVGAGVSGFKVGDRVAYAGRPNGAYAEERVMPADILVKLPDAISFDTAA 118 Query: 124 ALPVASVAAWQSLNFAGPIQAGQRILIHGAAGGLGGFAVQYAHQAGA-VVFAAALEEHAG 182 A+ + + L +Q G IL H AAGG+G A Q+A G ++ + +E A Sbjct: 119 AMMLQGMTVQYLLRQTYAVQPGDTILFHAAAGGIGLIACQWARALGVNLIGTVSSDEKAA 178 Query: 183 YLRSLGADHVIAYDKEQFE------ASARDIDLVLDYVGGEVLNRSWSVLAEDGVIVSTA 236 GA HVI Y E F R + +V D +G + S L G++VS Sbjct: 179 LALQHGAAHVINYKTENFVERVKELTGGRGVQVVYDSIGKDTFLGSLDCLRPRGMMVSFG 238 Query: 237 SPAIQASTPA--------GRRGLWFMN-------TSDGARLQAIAEDVAHGRLHSKI 278 + AS P RG F+ TS A L A A D+ L +KI Sbjct: 239 N----ASGPVAPFGVSELASRGSLFLTRPSLGNYTSTRAELDATAGDLFDMVLGNKI 291 >gi|300310417|ref|YP_003774509.1| zinc-containing alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 72.8 bits (177), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 68/199 (34%), Positives = 99/199 (49%), Gaps = 19/199 (9%) Query: 15 PSALKISSIEAPVAGAGQVLVRVQAAGVNGLDWKVREGYVRDAFPLQLPTVPGIELAGIV 74 P AL +++ P G +LV V+A VN +D K+R ++A V G +++G+V Sbjct: 16 PDALIETTLPDPTPGEHDLLVEVKAISVNPVDTKIR-ASAKEA------RVLGWDVSGVV 68 Query: 75 EAAG-AGTRLRKGDRVM--GPRGGLGAYADLVAVDEANLCLIPESLSFVEAAALPVASVA 131 A G A T GD V G GA ++L VDE + P SL +AAA+P+ ++ Sbjct: 69 RAVGPAVTLFAPGDEVFYAGSITRPGANSELHVVDERIVGRKPSSLGHADAAAIPLTAIT 128 Query: 132 AWQSLNFAGPIQ----AGQRILIHGAAGGLGGFAVQYAHQAG--AVVFAAALEEHAGYLR 185 AW+ L + GQ +LI GAAGG+G Q A + V+ A+ E A ++ Sbjct: 129 AWELLFDRVAVAEGGGQGQHLLIIGAAGGVGSMLTQLARKLTQLTVIGTASRPETAHWVG 188 Query: 186 SLGADHVIAYD---KEQFE 201 LGA HVI + K Q E Sbjct: 189 ELGAHHVIDHSQPLKPQLE 207 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 932,792 Number of extensions: 38895 Number of successful extensions: 183 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 171 Number of HSP's successfully gapped: 5 Length of query: 309 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 224 Effective length of database: 1,217,395 Effective search space: 272696480 Effective search space used: 272696480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)