BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0029.p (127 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312793|ref|YP_003776885.1| TetR family transcription regul... 98 2e-22 >gi|300312793|ref|YP_003776885.1| TetR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 188 Score = 97.8 bits (242), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 53/137 (38%), Positives = 78/137 (56%), Gaps = 16/137 (11%) Query: 3 RHFPTREALFDALLRTNLDALTQKASEFESSHPPDEALVSWFREGVAFVHSYSGVVALMA 62 RHFPTREAL +ALLR++ +AL ++A E +++ AL+ W +E +AF H + GV+A M Sbjct: 45 RHFPTREALLEALLRSSFEALAERADELQAADDSAAALMEWLQEIIAFTHEHRGVIAPMV 104 Query: 63 RAHEDPNS-----------DGIDLFALXN-----WADMDGIDLFALMSALGWVGDQPSFA 106 ED S G L + AD+DG +LF L++AL W+ +QPS A Sbjct: 105 SVIEDHESALHASCVSLRAAGAALLSRAQADGKARADLDGAELFDLIAALAWLREQPSHA 164 Query: 107 PRGDHLFHVITSAILTN 123 R D +F +I+ A+L Sbjct: 165 SRADRIFGIISGAVLNT 181 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 414,947 Number of extensions: 15841 Number of successful extensions: 51 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 50 Number of HSP's successfully gapped: 1 Length of query: 127 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 52 Effective length of database: 1,264,745 Effective search space: 65766740 Effective search space used: 65766740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 125 (52.8 bits)