BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0028.p (256 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 219 2e-58 gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogena... 211 6e-56 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 113 1e-26 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 112 2e-26 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 112 4e-26 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 219 bits (557), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 121/244 (49%), Positives = 157/244 (64%), Gaps = 4/244 (1%) Query: 2 NRLHQKTAVITGGATGIGLAAAKRFIEEGAFVFIFGRRQEALDAAMADLGPNARAVKGSI 61 R K V+TGG+ GIGLA AK F EGA V++ GRR + L+ A+ ++G A V G Sbjct: 5 KRFEDKVVVVTGGSEGIGLATAKLFAAEGARVYVSGRRADKLEQAVREIGNGAVGVPGDA 64 Query: 62 TDPDDLDRLYAAVKAERGTLDIVFANAGAGGP--LPLGKLTAEHIDETFDTNVKGTIFTV 119 DDLDRLYA V+A+ G LD++FANAG PLG + E D F+ NV+G +F+V Sbjct: 65 AKLDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFNLNVRGLLFSV 124 Query: 120 QKALPLMGQGGSIILTGSSAGTTGAPAFSVYSASKAAVRNLARTWAEDLKGTGIRVNVLS 179 QKALPLM QGG+I+L GS AG G P S+Y+ASKAAVR+ AR+W DLK GIRVNV++ Sbjct: 125 QKALPLMSQGGAIVLNGSVAGVKGFPGQSLYNASKAAVRSFARSWTADLKERGIRVNVVA 184 Query: 180 PGPTATELAKAAL-GEEGMK-VFAAMNPLQRMADPAELGAAAAFLASQDSSFMTASEVAV 237 PG T T L + L GM+ V + PL R+ +P E+G A FLAS +SS++ E+ V Sbjct: 185 PGGTETRLMRDYLEARPGMEDVLKQVVPLGRLGEPDEIGRAVLFLASAESSYIAGVELFV 244 Query: 238 DXDS 241 D S Sbjct: 245 DGGS 248 >gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 211 bits (536), Expect = 6e-56, Method: Compositional matrix adjust. Identities = 126/261 (48%), Positives = 161/261 (61%), Gaps = 17/261 (6%) Query: 1 MNR-LHQKTAVITGGATGIGLAAAKRFIEEGAFVFIFGRRQEALDAAMADLGPNARAVKG 59 MN+ L+ K A++TGG +GIGLA A+ +GA VFI GRRQ LDAA+A +G A ++ Sbjct: 1 MNKQLNGKIALVTGGTSGIGLATAQELAAQGAQVFITGRRQAELDAAVASIGAAATGIRA 60 Query: 60 SITDPDDLDRLYAAVKAERGTLDIVFANAGAGGPLPLGKLTAEHIDETFDTNVKGTIFTV 119 + +LD +YA + G LDI+FANAG G +PLG +T EH D F TNV+G +FTV Sbjct: 61 DASVLSELDTVYAQIAKSAGRLDILFANAGGGDMMPLGAITEEHFDRIFGTNVRGVLFTV 120 Query: 120 QKALPLMGQGGSIILTGSSAGTTGAPAFSVYSASKAAVRNLARTWAEDLKGTGIRVNVLS 179 QKALPL+ G S+ILT S+ G FSVYSASKAAVRN AR+WA DLK IRVNV+S Sbjct: 121 QKALPLLVDGASVILTSSTTSVLGTANFSVYSASKAAVRNFARSWALDLKDRAIRVNVVS 180 Query: 180 PGPTAT----ELAKAALGEEGMKVFAAMNPLQRMADPAELGAAAAFLASQDSSFMTASEV 235 PGP T EL + A+ PL R+ +P E+G A AFLAS +SF+ E+ Sbjct: 181 PGPIRTPGLGELVPDDARQGLFDYLASQVPLGRLGEPQEIGKAVAFLASDAASFVNGIEL 240 Query: 236 AVDXDSSFMTASEVAGGLAQI 256 VD GG+AQ+ Sbjct: 241 FVD------------GGMAQV 249 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 113 bits (283), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 86/250 (34%), Positives = 130/250 (52%), Gaps = 12/250 (4%) Query: 1 MNRLHQKTAVITGGATGIGLAAAKRFIEEGAFVFIFGRRQEALD---AAMADLGPNARAV 57 MN L K A+ITG + GIG A A F GA + + RRQ+AL+ A + G A V Sbjct: 1 MNTLQGKVALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRASVV 60 Query: 58 KGSITDPDDLDRLYAAVKAERGTLDIVFANAGA-GGPLPLGKLTAEHIDETFDTNVKGTI 116 G + + +R A E G LDI NAGA G PL +++ + +T N+ Sbjct: 61 AGDVGEAATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTSAF 120 Query: 117 FTVQKALP-LMGQG-GSIILTGSSAGTT-GAPAFSVYSASKAAVRNLARTWAEDLKGTGI 173 + +P ++ +G G+I+ T S G++ G P + Y A+KA + L + A D GI Sbjct: 121 LGARCQIPAMLARGAGAIVFTSSFVGSSAGLPGMAAYGAAKAGLMGLVKGIAADYAVQGI 180 Query: 174 RVNVLSPGPTATELAKAALGEEGMKVFAA-MNPLQRMADPAELGAAAAFLASQDSSFMTA 232 R N L PG T++ G+ K +AA ++ ++R+A P E+ AAA FLAS +SF+T Sbjct: 181 RANALLPGGVDTDMG----GDPQQKQWAAGLHAMKRIAQPEEIAAAALFLASPMASFVTG 236 Query: 233 SEVAVDXDSS 242 + + D +S Sbjct: 237 TALYADGGNS 246 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 112 bits (281), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 85/244 (34%), Positives = 118/244 (48%), Gaps = 12/244 (4%) Query: 4 LHQKTAVITGGA--TGIGLAAAKRFIEEGAFVFIFGRRQEALDAAMADLGPNARAVKGSI 61 L K AVITGGA G+G A A+ E+GA V + Q AA A +G + + Sbjct: 3 LKDKVAVITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAARIGSQHLGLVADV 62 Query: 62 TDPDDLDRLYAAVKAERGTLDIVFANAGAGGPLPLGKLTAEHIDETFDTNVKGTIFTVQK 121 TD + AA+ + G +DI+ NAG P+ ++T D D +++GT++ Q Sbjct: 63 TDKASCETAAAAILQQFGRIDILVNNAGITQPVKTLEITGADYDRILDVSLRGTLYMSQA 122 Query: 122 ALPLM--GQGGSIILTGSSA-----GTTGAPAFSVYSASKAAVRNLARTWAEDLKGTGIR 174 LP M Q GSII S + G G P YSA+KA V LAR A + GIR Sbjct: 123 VLPAMRAQQNGSIICISSVSAQRGGGILGGPH---YSAAKAGVLGLARAMAREFGPEGIR 179 Query: 175 VNVLSPGPTATELAKAALGEEGMKVFAAMNPLQRMADPAELGAAAAFLASQDSSFMTASE 234 VN ++PG T++ K L EE A PL R+ ++G A FL S S + T Sbjct: 180 VNSITPGLIGTDIIKGKLTEEKKAEIAETIPLARLGRADDIGGACVFLGSDLSQYCTGIT 239 Query: 235 VAVD 238 + V+ Sbjct: 240 LDVN 243 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 112 bits (279), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 82/244 (33%), Positives = 118/244 (48%), Gaps = 12/244 (4%) Query: 4 LHQKTAVITGGA--TGIGLAAAKRFIEEGAFVFIFGRRQEALDAAMADLGPNARAVKGSI 61 L K A+ITGGA G+G + A+ +GA + I + A A LG + + Sbjct: 3 LKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAARLGAQHLGLVADV 62 Query: 62 TDPDDLDRLYAAVKAERGTLDIVFANAGAGGPLPLGKLTAEHIDETFDTNVKGTIFTVQK 121 TD + AAV + G +DI+ NAG P L ++T + D D +++GT++ Q Sbjct: 63 TDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGTLYMSQA 122 Query: 122 ALPLMG--QGGSIILTGSSA-----GTTGAPAFSVYSASKAAVRNLARTWAEDLKGTGIR 174 LP M Q GSI+ S + G G P YSA+KA V LAR A + GIR Sbjct: 123 VLPAMQAQQSGSIVCISSVSAQRGGGILGGPH---YSAAKAGVLGLARAMAREFGPQGIR 179 Query: 175 VNVLSPGPTATELAKAALGEEGMKVFAAMNPLQRMADPAELGAAAAFLASQDSSFMTASE 234 VN ++PG +T++ K L E A PL R+ P ++G FLAS S + T Sbjct: 180 VNCITPGLISTDINKDILSAEKKAEIAQTIPLGRLGGPDDVGGVCVFLASDLSQYCTGVT 239 Query: 235 VAVD 238 + V+ Sbjct: 240 LDVN 243 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.131 0.362 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 613,952 Number of extensions: 21591 Number of successful extensions: 193 Number of sequences better than 1.0e-08: 49 Number of HSP's gapped: 167 Number of HSP's successfully gapped: 49 Length of query: 256 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 173 Effective length of database: 1,226,865 Effective search space: 212247645 Effective search space used: 212247645 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)