BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0024.p (481 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 412 e-116 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 384 e-108 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 371 e-104 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 325 7e-90 gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipo... 213 3e-56 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 412 bits (1058), Expect = e-116, Method: Compositional matrix adjust. Identities = 229/460 (49%), Positives = 305/460 (66%), Gaps = 8/460 (1%) Query: 16 LISASLAACSLAPSYQQPAFPVAAHWQNDLQKAEPAGTQSDTRQAASIAWTDFFQDAQLK 75 +++ L CSLAP+Y++P PV + + A+ AG QA ++ W FF D +L+ Sbjct: 20 VMAGVLGGCSLAPTYERPQAPVESSY-----PADAAGRAE--LQAVNLGWRQFFPDQRLQ 72 Query: 76 SVIAAALDHNRDLRMATLNIERARAMYNIQVADQVPNLALNGNGARQRLPADVNPTGKAG 135 +IAAAL++NRDLR A LNIE ARA Y I AD +PNL L+GN AR R A + TG+ Sbjct: 73 QLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAARTRTAASQSLTGQPT 132 Query: 136 VNSSYFAGVGLAAFEIDLFGRVRNLSEAALQRYLATEQAQRSAQISLIAEVANTYEALVA 195 +++SY G+ + A+E+D FGRVRNL +AAL YL+TE+AQ+SAQISL+A+VA Y A + Sbjct: 133 ISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQVAQAYLAERS 192 Query: 196 DRRLLQLTENTLASRADSYERQKQLYAEGASSEYDLRQAESLFEAAKAARAQQERRKTLD 255 L+L + TL SR + + ++ GAS+ D R ++L E+A+ A+ Q ER++ Sbjct: 193 YAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESARVAKTQLERQRAQA 252 Query: 256 ENALTVLVGHPLAADIGTANATSAEQVLPDLPAGLPSDLLTARPDILQQEAVLRSQNANI 315 ENALT+LVG + ++ A S E+++ D+PAGLPSDLLT RPDI E L + NANI Sbjct: 253 ENALTLLVGKKIG-ELPPAQLLSDEKIVTDIPAGLPSDLLTNRPDIRSAEQSLLAANANI 311 Query: 316 GAARAAFFPRISLTGNFGSASSQLSGLFDSGSRSWSFIPQISIPIFEGGRNIANLNVAET 375 GAARAAFFPRISLTGNFGSASS L GLF+SGS +WSF P +S+PIF+ GRN ANL++AE Sbjct: 312 GAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFDFGRNSANLDLAEV 371 Query: 376 DKKIAVAQYEKTVQVAFREVADALAGRATLADEAGAVKAQEAAESARYGFARSRYEAGYS 435 K AV YEK +Q AFREVADAL R +L ++ A +A AA+ R R++ G + Sbjct: 372 RKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAAQQERLKLTDLRFKNGIA 431 Query: 436 SYLEYLDAQRSLFAIQQQSIQVQLAELQTRITLYKVLGGG 475 S L+ LDAQR LF+ QQ IQ + L I LYK LGGG Sbjct: 432 SSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGG 471 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 384 bits (986), Expect = e-108, Method: Compositional matrix adjust. Identities = 216/472 (45%), Positives = 296/472 (62%), Gaps = 20/472 (4%) Query: 16 LISASLAACSLAPSYQQPAFPVAAHWQNDLQKAEPAGTQSDTRQAASIAWTDFFQDAQLK 75 +++ L C+L P Y +P PVAAH+ ++ A S A I W D F D L+ Sbjct: 19 MLAMLLGGCTLMPDYHRPDAPVAAHFAG--ADSKNAAGASSAPVVADIGWRDVFTDPSLQ 76 Query: 76 SVIAAALDHNRDLRMATLNIERARAMYNIQVADQVPNLALNGNGARQRLPADVNPTGKAG 135 VIA AL++NRDLR+A LNIE+ARA Y +Q A P++ + R P D++ TG A Sbjct: 77 QVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAATSETASRTPGDLSSTGSAL 136 Query: 136 VNSSYFAGVGLAAFEIDLFGRVRNLSEAALQRYLATEQAQRSAQISLIAEVANTYEALVA 195 + SY A +G +A+E+DLFGRVR+L E ALQ +L+T +A+RS QISLIAEVA + L + Sbjct: 137 TSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQISLIAEVATAWLTLAS 196 Query: 196 DRRLLQLTENTLASRADSYERQKQLYAEGASSEYDLRQAESLFEAAKAARAQQERRKTLD 255 D+ L+L ++TL S++ SY ++ + G+SS LRQA++ E+A+ + D Sbjct: 197 DQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTSVESARVDVESYTAQVDQD 256 Query: 256 ENALTVLVGHPLAADIGTANATSAEQVLPD-----------LPAGLPSDLLTARPDILQQ 304 NAL +LVG D+ TA Q LPD +P GLPSDLL R DI+Q Sbjct: 257 RNALVLLVGK----DLPTA---LLPQGLPDTRLAAASPLATIPPGLPSDLLQRRADIVQA 309 Query: 305 EAVLRSQNANIGAARAAFFPRISLTGNFGSASSQLSGLFDSGSRSWSFIPQISIPIFEGG 364 E LR+ NANIGAARAAF+P I+LT N G+AS++L+GLF GS SWSF+PQIS+PIF+GG Sbjct: 310 ERDLRAANANIGAARAAFYPSITLTVNAGTASARLAGLFKGGSGSWSFVPQISLPIFDGG 369 Query: 365 RNIANLNVAETDKKIAVAQYEKTVQVAFREVADALAGRATLADEAGAVKAQEAAESARYG 424 N ANL++A + I+VAQYEK +Q AFREV+DALA R TL + A +A A + Sbjct: 370 ANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQEALVQASDEAFR 429 Query: 425 FARSRYEAGYSSYLEYLDAQRSLFAIQQQSIQVQLAELQTRITLYKVLGGGW 476 +++RY +G SYL LD+QR+L++ Q+ I QL+ +T YK LGGGW Sbjct: 430 LSQARYHSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYKTLGGGW 481 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 371 bits (953), Expect = e-104, Method: Compositional matrix adjust. Identities = 213/465 (45%), Positives = 286/465 (61%), Gaps = 9/465 (1%) Query: 23 ACSLAPSYQQPAFPVAAHW------QNDLQKAEPAGTQSDTRQAASIAWTDFFQDAQLKS 76 +LAP Y QPA PV A W Q + A PA + D A + W +FF D +L+ Sbjct: 28 CANLAPDYTQPAAPVPASWTSQAAAQPAVGSAGPAAGEID---ADHLGWREFFLDPRLQQ 84 Query: 77 VIAAALDHNRDLRMATLNIERARAMYNIQVADQVPNLALNGNGARQRLPADVNPTGKAGV 136 VIAAALD+NRDLR+A LNIE+ARA Y I A++ P+++ G + R PA+++ TG A V Sbjct: 85 VIAAALDNNRDLRVAALNIEKARAQYRITDANRYPSISATGTSSASRTPAELSTTGSAAV 144 Query: 137 NSSYFAGVGLAAFEIDLFGRVRNLSEAALQRYLATEQAQRSAQISLIAEVANTYEALVAD 196 + VG++A+E+D FGR++NLS+AAL+ Y + +RS ISL+AEVA + L +D Sbjct: 145 SHQQSVTVGVSAYELDFFGRLKNLSDAALESYQYQVETRRSTHISLVAEVATAWLTLASD 204 Query: 197 RRLLQLTENTLASRADSYERQKQLYAEGASSEYDLRQAESLFEAAKAARAQQERRKTLDE 256 R LL+L ++T AS++ ++ ++ + G S DL E+ E+A+A A+ + D Sbjct: 205 RDLLKLAQDTYASQSRTFALSQRSHDIGTVSGVDLASQEATVESARADVARYASQVEQDL 264 Query: 257 NALTVLVGHPLAADIGTANATSAEQVLPDLPAGLPSDLLTARPDILQQEAVLRSQNANIG 316 NAL +LVG + A + A VLP PAGLPS +L RPD+L E L+S NA+IG Sbjct: 265 NALRLLVGADIDAALLPAGLPDTASVLPATPAGLPSQVLLRRPDVLAAEHTLKSANADIG 324 Query: 317 AARAAFFPRISLTGNFGSASSQLSGLFDSGSRSWSFIPQISIPIFEGGRNIANLNVAETD 376 AARAAFFP ISLT N G+AS L GLF +GS SW+F P I++PIF G A+L+ A+ Sbjct: 325 AARAAFFPSISLTANGGTASRTLGGLFKAGSGSWTFSPSINLPIFNAGSLRASLDSAKIT 384 Query: 377 KKIAVAQYEKTVQVAFREVADALAGRATLADEAGAVKAQEAAESARYGFARSRYEAGYSS 436 + I VA YEK +Q AFREVADALA RATL A AA + + +RY G S Sbjct: 385 RDIDVANYEKAIQTAFREVADALAVRATLDQRLDAQGKATAASEKAFRLSDARYRNGVDS 444 Query: 437 YLEYLDAQRSLFAIQQQSIQVQLAELQTRITLYKVLGGGWKMAKQ 481 YL L AQR+L++ QQ I ++L E RITLYKVLGGGW Q Sbjct: 445 YLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVLGGGWSEQTQ 489 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 325 bits (832), Expect = 7e-90, Method: Compositional matrix adjust. Identities = 195/453 (43%), Positives = 281/453 (62%), Gaps = 3/453 (0%) Query: 23 AC-SLAPSYQQPAFPVAAHWQNDLQKAEPAGTQSDTRQAASIAWTDFFQDAQLKSVIAAA 81 AC SLAP YQ+PA PVAA + D E A ++ + + W D+F D L+++I A Sbjct: 24 ACQSLAPHYQRPAAPVAAVYP-DGPVNEAAHQVANEAASPPLPWRDYFTDPPLQALITQA 82 Query: 82 LDHNRDLRMATLNIERARAMYNIQVADQVPNLALNGNGARQRLPADVNPTGKAGVNSSYF 141 L+++RDLR A L ++ A+A+Y I AD P + R R PAD+N T + V S+Y Sbjct: 83 LENSRDLRTAALRVQEAQALYGIGHADLFPTIGAQAGLDRSRTPADLNLTRRPLVGSAYQ 142 Query: 142 AGVGLAAFEIDLFGRVRNLSEAALQRYLATEQAQRSAQISLIAEVANTYEALVADRRLLQ 201 AG+GL+++EIDL+GR+R+L +AAL+ A++ + + +SL+A+VA+ + L L+ Sbjct: 143 AGLGLSSWEIDLWGRLRSLDDAALESARASQATRSAVALSLMAQVADAWLELAELDERLE 202 Query: 202 LTENTLASRADSYERQKQLYAEGASSEYDLRQAESLFEAAKAARAQQERRKTLDENALTV 261 + T+ASR +S+ + GA+S +L Q E+L A+A AQ ++++ NALT+ Sbjct: 203 IANQTIASRQESFRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNALTL 262 Query: 262 LVGHPLAADIGTANATSAEQVLPDLPAGLPSDLLTARPDILQQEAVLRSQNANIGAARAA 321 LVG P+ +G A A + V L GLP+ LLT RPD+ E L + NANIGAARAA Sbjct: 263 LVGSPVVLPVGQARA-GLDAVFAPLQPGLPALLLTRRPDVAAAEHRLMAANANIGAARAA 321 Query: 322 FFPRISLTGNFGSASSQLSGLFDSGSRSWSFIPQISIPIFEGGRNIANLNVAETDKKIAV 381 FFP ISLTGN G+AS++LSGLF GS +W+F P I++PIF GR NL ++E + +AV Sbjct: 322 FFPSISLTGNVGTASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVRRDLAV 381 Query: 382 AQYEKTVQVAFREVADALAGRATLADEAGAVKAQEAAESARYGFARSRYEAGYSSYLEYL 441 A YEKTVQ AFREVADALA R L + ++ + R + RY+ G + +L+ L Sbjct: 382 AAYEKTVQTAFREVADALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAPFLDVL 441 Query: 442 DAQRSLFAIQQQSIQVQLAELQTRITLYKVLGG 474 DAQR L + QQQ +Q++ A L + + LY LGG Sbjct: 442 DAQRDLLSAQQQLVQIRRALLSSSVALYAALGG 474 >gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 481 Score = 213 bits (542), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 159/472 (33%), Positives = 251/472 (53%), Gaps = 12/472 (2%) Query: 6 QSLRTIAGISLISASLAACSLAPSYQQPAFPVAAHWQNDLQKAEPAGTQSDTRQAASIAW 65 + LR + ++L + + L PS A + H+ L + PA A++I W Sbjct: 7 RHLRLLPTLALCACAPLTAPLPPS----ALDLPTHYPRTLAEDAPANAAPT---ASAIGW 59 Query: 66 TDFFQDAQLKSVIAAALDHNRDLRMATLNIERARAMYNIQVADQVPNLALNGNGARQRLP 125 D+F A L+++IA AL HN DLR L +++ +A + IQ + +P + L R R P Sbjct: 60 QDYFPQAPLQALIAEALRHNPDLRDIRLRLQQVQAQWRIQRSTGLPTVNLEAQARRSRGP 119 Query: 126 ADVNPTGKAGVNSSYFAGVGLAAFEIDLFGRVRNLSEAALQRYLATEQAQRSAQISLIAE 185 + +G A Y G+G +++E+D +GR+ +L+EAALQ ++A+ A+++ ISLI + Sbjct: 120 LEPPESGPAASTREYRVGLGASSWELDFWGRIASLNEAALQEWMASRAAEQALVISLIGQ 179 Query: 186 VANTYEALVADRRLLQLTENTLASRADSYERQKQLYAEGASSEYDLRQAESLFEAAKAAR 245 VA + +L L L E A+ A+S + + G + L Q S +A + Sbjct: 180 VAAAWTSLRELDERLHLLETLQAATAESLALMQARHDAGRADAAALAQQRSELAQMRAEQ 239 Query: 246 AQQERRKTLDENALTVLVG-HPLAADIGTANATSAEQVLP-DLPAGLPSDLLTARPDILQ 303 A+ ++ + + L +L G L A + A T +LP L GLPS LL ARPDI+ Sbjct: 240 AELQQMRASQRSTLEMLAGLTQLPAALEPAPTTI---LLPFALAPGLPSTLLAARPDIIA 296 Query: 304 QEAVLRSQNANIGAARAAFFPRISLTGNFGSASSQLSGLFDSGSRSWSFIPQISIPIFEG 363 E L++ A + AARAAFFPRISLT G AS+ L LF SR+W+F+P+IS+P+ +G Sbjct: 297 AEHRLQASAAQVAAARAAFFPRISLTAFGGVASTALQDLFKGPSRAWNFVPEISLPLLDG 356 Query: 364 GRNIANLNVAETDKKIAVAQYEKTVQVAFREVADALAGRATLADEAGAVKAQEAAESARY 423 G + ++A D++ A+ YE+TVQ AFREVA L L ++ + ++ R Sbjct: 357 GLRQSRYDIAALDQQRALLDYERTVQNAFREVAQRLEEAHWLKEQLRLRREVVQQQAQRQ 416 Query: 424 GFARSRYEAGYSSYLEYLDAQRSLFAIQQQSIQVQLAELQTRITLYKVLGGG 475 R G +S L+ L+++R QQ+ ++ A L+ +I LY LGGG Sbjct: 417 AQMHMRLRYGRASRLDVLESERDHLRAQQRQHMLEHALLRNQIALYVALGGG 468 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.128 0.348 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,269,795 Number of extensions: 49464 Number of successful extensions: 279 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 245 Number of HSP's successfully gapped: 18 Length of query: 481 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 392 Effective length of database: 1,198,455 Effective search space: 469794360 Effective search space used: 469794360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)