BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0022.p (324 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309610|ref|YP_003773702.1| cation/multidrug efflux protein... 195 4e-51 gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump pr... 67 2e-12 gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance ... 63 3e-11 >gi|300309610|ref|YP_003773702.1| cation/multidrug efflux protein [Herbaspirillum seropedicae SmR1] Length = 1047 Score = 195 bits (496), Expect = 4e-51, Method: Compositional matrix adjust. Identities = 92/242 (38%), Positives = 150/242 (61%) Query: 4 FNLSAFGVRERAVTLFLIIAIAVAGAFAFIKLGRAEDPSFTVKIMTVTALWPGATAKEMQ 63 FNLSA+ ++ +A+ F++ + + G ++ +L ++EDP FT K+M + WPGATA+++Q Sbjct: 7 FNLSAWTLQHQALVTFILALVTLLGIGSYSRLSQSEDPPFTFKVMVIQTDWPGATARQVQ 66 Query: 64 QQVGDRLEKRLQELEYYDRVETAARPGLITMKLFLKDSTLPALVPEQFYQARKKLGDEVI 123 +Q+ DR+ ++LQE D + + +R G + +KD+ + VP+ +YQ RKK+GD Sbjct: 67 EQITDRIARKLQETPDVDFLRSYSRAGQSQLFFTIKDTAPASQVPQTWYQVRKKVGDMAA 126 Query: 124 NMPRGAIGPVINDEYSDVYFTLFSLNAPGLPHRQLVTEAETLRQQLLHVDGVQKVNILGE 183 +P+G GP NDE+ DVY +++L G QL A+ LR +LLHV GV K++ GE Sbjct: 127 TLPQGVKGPYFNDEFGDVYTNIYALAGDGFSPAQLRDYADQLRGELLHVPGVAKIDYFGE 186 Query: 184 QQQKIYVDISNQRLATLGVKAQAIFDALNRQNDMTPSGFVETAGPRVYLRLDGAINNIDA 243 + + IY+DI N R+ LG+ Q + DA+ QN + P G + T RVY+R G +N+ A Sbjct: 187 RNEHIYIDIGNARMTRLGISPQQLADAIGGQNAVVPGGLITTGNDRVYVRPTGQFDNLRA 246 Query: 244 VK 245 ++ Sbjct: 247 LE 248 >gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1063 Score = 66.6 bits (161), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 54/249 (21%), Positives = 113/249 (45%), Gaps = 6/249 (2%) Query: 4 FNLSAFGVRERAVTLFLIIAIAVAGAFAFIKLGRAEDPSFTVKIMTVTALWPGATAKEMQ 63 N+ A +R + + + I +A + P + ++++ + G +A+EM Sbjct: 1 MNMVALALRRPYTFIVMALLIVLATPLTLKNMATDIFPEINIPVVSIIWNYNGLSAQEMG 60 Query: 64 QQVGDRLEKRLQEL-EYYDRVETAARPGLITMKLFLKDSTLPALVPEQFYQARKKLGDEV 122 Q++ + E+ L + + +E+ + G+ +K+F + A + Q + +V Sbjct: 61 QRIAAQNERGLTTVVSDIEHIESQSLAGVSVIKVFFQPK---ANIQTAIAQVVASVQTQV 117 Query: 123 INMPRGAIGP-VINDEYSDVYFTLFSLNAPGLPHRQLVTEA-ETLRQQLLHVDGVQKVNI 180 +P G P VI S + +L++P LP + + A +TLR QL+ + GV Sbjct: 118 RQLPPGITPPLVIKYSASSIPVMQLALSSPTLPEQTVFDAAVQTLRPQLVTIPGVAVPYP 177 Query: 181 LGEQQQKIYVDISNQRLATLGVKAQAIFDALNRQNDMTPSGFVETAGPRVYLRLDGAINN 240 G + + I VD+ Q L G+ + +A+N QN + PSG + ++++G+ Sbjct: 178 YGGKSRLISVDLDTQALQARGLAPIDVVNAINTQNLILPSGTAKFGATEYSVKINGSPEA 237 Query: 241 IDAVKAIPI 249 + + +P+ Sbjct: 238 VAGLNELPV 246 >gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance cation efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1073 Score = 63.2 bits (152), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 60/257 (23%), Positives = 114/257 (44%), Gaps = 23/257 (8%) Query: 9 FGVRERAVTLFLIIAIAVAGAFAFIKLGRAEDPSFTVKIMTVTALWPGATAKEMQQQVGD 68 F + R + L ++ + G +++ KL P T + + PG + E +Q++ Sbjct: 8 FAIEHRWLVLLAVLGMGAYGVYSYQKLPIDAVPDITNVQVQINTSAPGYSPLEAEQRISY 67 Query: 69 RLEKRLQELEYYDRVETAARPGLITMKLFLKDSTLPALVPEQFYQARKKLGDEVINMPRG 128 +E + L ++V + +R GL + + KD T Y AR+ L +E I + R Sbjct: 68 PIETLMSGLPNLEQVRSLSRYGLSQVTVIFKDGT-------DIYFARQ-LVNERIQLARS 119 Query: 129 AIGP-------VINDEYSDVY-FTLFSLNAPGLPHRQLVTEAE-------TLRQQLLHVD 173 + P I+ ++Y +T+ S + P T + ++ QL V Sbjct: 120 QLPPGIDPGMGPISTGLGEIYLWTVESRDDARKPDGTPYTATDLREIQDWVIKPQLRLVP 179 Query: 174 GVQKVNILGEQQQKIYVDISNQRLATLGVKAQAIFDALNRQNDMTPSGFVETAGPRVYLR 233 GV ++N +G ++ V S ++LA+ G+ I AL + N +G++E G + +R Sbjct: 180 GVTEINTIGGFAKEYLVAPSAEKLASYGMTMDTIIKALEKNNSNVGAGYIERRGEQYLIR 239 Query: 234 LDGAINNIDAVKAIPIA 250 G + +ID + I +A Sbjct: 240 APGQVASIDDIGNIVLA 256 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 940,279 Number of extensions: 36615 Number of successful extensions: 112 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 115 Number of HSP's successfully gapped: 3 Length of query: 324 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 239 Effective length of database: 1,217,395 Effective search space: 290957405 Effective search space used: 290957405 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)