BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0021.p (368 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309609|ref|YP_003773701.1| transport/efflux transmembrane ... 135 4e-33 gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbas... 61 2e-10 gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 59 6e-10 >gi|300309609|ref|YP_003773701.1| transport/efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 376 Score = 135 bits (341), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 122/353 (34%), Positives = 182/353 (51%), Gaps = 15/353 (4%) Query: 15 ALIATLAACSKPAPPPATAPR--VASFTVQNVEHDSASYTGVVHARTESNLGFRVPGKIV 72 AL+ TLAACSKPAP A APR V Q + + V +R +++ FR+ G++ Sbjct: 22 ALVITLAACSKPAPV-ADAPREVVVLQAQQRGQAGTLHLPAEVQSRYVTSMSFRIAGQLA 80 Query: 73 ERLVDPGELVKRGQALFKLDSTDYTLAQHAARSAVIAAQ----ARDVQAQADEVRLRKLL 128 ER V G++V++GQ L +LD D AA++ + +A+ A + Q Q D + R+ L Sbjct: 81 ERRVHLGDVVRKGQVLARLDPADANQNASAAQAELASARQHLDAAEKQLQRDVQQAREAL 140 Query: 129 NSGAVSRQAYEQSKALADSAKAQLDAERARAGQVENQSDYSVLRADADGVVMDVLADAGQ 188 +S Q EQS+ +A +Q A +ARA NQ DY+ L A DGV+ A+ G Sbjct: 141 ----ISPQQLEQSQDAHAAALSQFKAAQARAAVAGNQRDYTTLVAQHDGVISAEQANTGD 196 Query: 189 VVGAGQTIVRLAQKGPREAVVSIPENGLG--QARRQASATLYTDDTQKFAAKLRELSAMA 246 V+ AGQ + LA G + V + + + Q A TL + F K+RE+S A Sbjct: 197 VLAAGQPVYSLAWSGQVDVVTEVADRQVATLQPGAAAVVTLAALPGKTFTGKVREVSPAA 256 Query: 247 DPLTRTYQARYVLSGKGEQAPLGSTVTIRLTGDTTSTEQT-EVPIGALIDKGHGASVWLI 305 D +RTY+ + L LG T + +T +S +P AL +G ++W++ Sbjct: 257 DAQSRTYRVKVTLEQPDPAVRLGMTGEVAITPAASSAAPVLLLPATALFHQGDRPALWVV 316 Query: 306 EKQTQVVKLVPVTVVRLGEEFAAISAGLKAGDVVVAYGTHLLKAGDKVSVVSP 358 +Q Q ++L PVTV GE ++S G+ A D VV G H L AG+KV V+P Sbjct: 317 GEQGQ-LELRPVTVAAYGERSISVSQGVTASDKVVVQGVHTLTAGEKVKPVAP 368 >gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 60.8 bits (146), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 95/362 (26%), Positives = 147/362 (40%), Gaps = 47/362 (12%) Query: 29 PPATAPRVASFTVQNV-EHDSASY---TGVVHARTESNLGFRVPGKIVERLVDPGELVKR 84 P A AP S T+ +V E D Y G V A + R+ G++ + GE VK+ Sbjct: 41 PQAKAPPPVSVTLASVQERDLPQYLAGVGTVTANASVVVKTRIDGQLEKVGFQEGEDVKK 100 Query: 85 GQALFKLDSTDYTLAQHAARSAVIAAQARDVQAQADEVRLRKLLNSGAVSRQAYEQSKAL 144 GQ L ++D+ A + A + A+ D R L + A ++Q Sbjct: 101 GQLLAQIDARALQAQLAQAEAQRAKDSATLMNARLDLQRYTTLRSQDAATQQ-------- 152 Query: 145 ADSAKAQLDAERARAGQVEN--------------QSDYSVLRADADGVVMDVLADAGQVV 190 QLD ++A Q+E Q Y+ + A G V L D G +V Sbjct: 153 ------QLDTQKALVAQLEAAVKTDEAQVAYAKVQLSYTTINAPISGRVGARLVDPGNIV 206 Query: 191 GA--GQTIVRLAQKGPREAVVSIPEN---GLGQARRQASATL----YTDDTQKFAA--KL 239 A +V + Q P V ++PE G+ +A+R L Y D+Q+ A KL Sbjct: 207 HATDANGLVVINQIDPITLVFTLPEEAVAGINRAQRAHQQPLQVSAYARDSQEVLATGKL 266 Query: 240 RELSAMADPLTRTYQARYVLSGKGEQAPLGSTVTIRLTGDTTSTEQTEVPIGALIDKGHG 299 L + T Q + + G V RL + T VP A+ G Sbjct: 267 VLLDNQINTTNGTVQLKARFDNPDHKLWPGQYVNARLQMRSHGNALT-VPAAAIQRNQQG 325 Query: 300 ASVWLIEKQTQVVKLVPVTVVRLGEEFAAISAG--LKAGDVVVAYGTHLLKAGDKVSVVS 357 V+ I+++ Q ++ PV V R+ + A ++AG ++AG VV G + LK G ++ Sbjct: 326 TYVYAIDQEDQ-ARMQPVKVERIQDGIAVLAAGNAVQAGQRVVIDGQYKLKPGSRIVESK 384 Query: 358 PQ 359 PQ Sbjct: 385 PQ 386 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 58.9 bits (141), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 54/193 (27%), Positives = 87/193 (45%), Gaps = 6/193 (3%) Query: 6 KYMGMALSGALIATLAAC-SKPA----PPPATAPRVASFTVQNVEHDSASYTGVVHARTE 60 ++ + ++ A+ A L AC KPA P P + V + Q V + G V A Sbjct: 6 RFTRLTMTVAIAAALVACGQKPAAQGGPMPPSEVSVVTIAPQRVAITN-ELPGRVEAFRV 64 Query: 61 SNLGFRVPGKIVERLVDPGELVKRGQALFKLDSTDYTLAQHAARSAVIAAQARDVQAQAD 120 + + RV G + +R+ G VK GQ LFK+D Y + +A++ + AQA Q Sbjct: 65 AQVRARVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQTTLT 124 Query: 121 EVRLRKLLNSGAVSRQAYEQSKALADSAKAQLDAERARAGQVENQSDYSVLRADADGVVM 180 R + L+ AVS+Q Y+ + A A+A + A +A Y+ + + G + Sbjct: 125 AQRYKPLVEVNAVSKQEYDNAVAAQKQAEADVAAGKAAVQTASLNLGYATVTSPISGRIG 184 Query: 181 DVLADAGQVVGAG 193 L G +VG G Sbjct: 185 RALVTEGALVGQG 197 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.127 0.345 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 966,737 Number of extensions: 35463 Number of successful extensions: 198 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 193 Number of HSP's successfully gapped: 9 Length of query: 368 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 281 Effective length of database: 1,207,925 Effective search space: 339426925 Effective search space used: 339426925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 131 (55.1 bits)