BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0020.p (140 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310485|ref|YP_003774577.1| transcription regulator protein... 69 2e-13 >gi|300310485|ref|YP_003774577.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 145 Score = 68.6 bits (166), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 39/124 (31%), Positives = 65/124 (52%), Gaps = 5/124 (4%) Query: 10 CNLFATKQAARFITQLYERHLAPVNVTSSQVTLLAVIHERPEITMTELSEMVVMDRTSLV 69 C ++ R IT LY+ HL ++ +Q +L++ I + I L++ + MDR++L Sbjct: 6 CTCAPLRRLTRRITVLYDHHLQDCELSITQYSLISRIGRKGPIANITLAQDMGMDRSTLS 65 Query: 70 RAIQPLSRDGYV-----VQKSEAGSRKIGLSLSKAGTRKFLDALPHWRAAQKEYEELVGK 124 RA++PL G++ ++ R L LS+ G K A P+WR AQ E + L+G Sbjct: 66 RALKPLMAAGWIETVDLPADAQLDKRSFALQLSQDGRAKLEQARPYWRRAQDEIDRLLGP 125 Query: 125 DRAK 128 D A+ Sbjct: 126 DLAR 129 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.129 0.356 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 358,000 Number of extensions: 11575 Number of successful extensions: 45 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 44 Number of HSP's successfully gapped: 1 Length of query: 140 Length of database: 1,619,870 Length adjustment: 76 Effective length of query: 64 Effective length of database: 1,260,010 Effective search space: 80640640 Effective search space used: 80640640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)