BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0018.p (278 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 100 1e-22 gi|300309599|ref|YP_003773691.1| enoyl-CoA hydratase [Herbaspiri... 88 7e-19 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 86 3e-18 gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 79 5e-16 gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspiri... 76 3e-15 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 100 bits (250), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 79/259 (30%), Positives = 128/259 (49%), Gaps = 9/259 (3%) Query: 19 ILFEHRDDGVLLATLNRPEVMNATNARLHWELTKLWQVVKDDAATKVVVITGAGDRAFSA 78 IL E+RD G+ + TLNRP+ +N+ +H EL ++ D + +V+++TGAG R F A Sbjct: 6 ILLENRD-GIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAG-RGFCA 63 Query: 79 GGDL--QWVEEMVGNPQKIATVHQEASDLVYNMLNCDKPIVSAINGTAVGAGLAVALLAD 136 G DL + V+ ++ + LV ++ P++ A+NG A GAG + L D Sbjct: 64 GQDLGDRAVKPGDDGVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPLACD 123 Query: 137 VSVIAEDARITDGHVKLGVAAGDHAAIVWPLLCGMAKAKYYLMTADFIDGKEAERLGLVT 196 + + A A + KLG+ P L G A+A + + I ++AER GL+ Sbjct: 124 IVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWGLIW 183 Query: 197 FCEPPSEVLPKALAVAAKLARGSQTAIRATKMSLNNWMRMAGPIFDNA-LAQEMLNFLG- 254 C +++ +A A+AA AR + ATK +L + A + + L ++ + LG Sbjct: 184 KCVDDDQLMEEAHAMAAHFARAPTKGLAATKATL--YASPAHTLPEQLDLERDTMRALGR 241 Query: 255 -EDAREGLAAVRSKRQADF 272 D REG+ A KR F Sbjct: 242 SRDYREGVTAFLEKRAPQF 260 >gi|300309599|ref|YP_003773691.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 88.2 bits (217), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 74/255 (29%), Positives = 124/255 (48%), Gaps = 17/255 (6%) Query: 24 RDDGVLLATLNRPEVMNATNARLHWELTKLWQVVKDDAATKVVVITGAGDRAFSAGGDLQ 83 + DGVL +R + NA A ++ +L + + D+A + ++ TG +AFSAG DL Sbjct: 9 KADGVLTIHFDREDKKNAITAAMYQQLADALKEAESDSAVRAILFTG-KPKAFSAGNDL- 66 Query: 84 WVEEMVGNPQKI--ATVHQEASDLVYNMLNCDKPIVSAINGTAVGAGLAVALLADVSVIA 141 E+ + NP K A V Q ++ + + KPIV+A++G AVG G + L D+ A Sbjct: 67 --EDFLKNPPKTQDAPVFQ----FLWQISHASKPIVAAVSGVAVGIGTTLLLHCDLVYAA 120 Query: 142 EDARITDGHVKLGVAAGDHAAIVWPLLCGMAKAKYYLMTADFIDGKEAERLGLVTFCEPP 201 ++A+ + +LG+ ++++ P + G +A L+ + EA ++G V Sbjct: 121 DNAKFSMPFTQLGLCPEAASSLLLPHIAGYQRAAEKLLLGEAFGADEACQMGFVNRVMAQ 180 Query: 202 SEVLPKALAVAAKLARGSQTAIRATKMSLNNWMRMAGPIFDNALAQEMLNF----LGEDA 257 E+LP A AAKLA +IR TK + A + + E+ +F +A Sbjct: 181 EELLPYAQQQAAKLAALPAASIRVTKQLMKKNRVTAT---EQQMVAEIEHFSRMLTAPEA 237 Query: 258 REGLAAVRSKRQADF 272 +E A KR+ DF Sbjct: 238 KEAFTAFFEKRKPDF 252 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 85.9 bits (211), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 78/263 (29%), Positives = 126/263 (47%), Gaps = 13/263 (4%) Query: 13 YEDFQHILFEHRDDGVLLATLNRPEVMNATNARLHWELTKLWQVVKDDAATKVVVITGAG 72 YED +L + VL TLNRP + NA + E+ ++D + +VVVI+G Sbjct: 7 YED---LLAARHGEHVLHLTLNRPALRNALRNQSLREIVAALARAEEDDSVRVVVISG-N 62 Query: 73 DRAFSAGGDLQWVEEMVGNPQKIATVHQEASDLVYNMLNCDKPIVSAINGTAVGAGLAVA 132 ++AF+AG DL EM+ + IAT + + KPI++A+NG A+GAG + Sbjct: 63 EKAFAAGADLN---EMI-HKDAIATQLDVRAQYWRAIARFPKPILAAVNGYALGAGCELL 118 Query: 133 LLADVSVIAEDARITDGHVKLGVAAGDHAAIVWPLLCGMAKAKYYLMTADFIDGKEAERL 192 + AD+++ A A+I + +G G G A +++ +FI +A + Sbjct: 119 MHADIAIAARGAKIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQA 178 Query: 193 GLVTFCEPPSEVLPKALAVAAKLARGSQTAIRATKMSLNNWMRM---AGPIFDNALAQEM 249 GLV L + LA+A +A+ S A+R K ++ + AG +F+ M Sbjct: 179 GLVAEVVDDDATLERTLALAHSIAQKSPLAVRLAKEAMLQSFELGLEAGLLFERKSFSLM 238 Query: 250 LNFLGEDAREGLAAVRSKRQADF 272 D +EG+AA + KR A F Sbjct: 239 AA--SADRQEGIAAFQEKRAAVF 259 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 78.6 bits (192), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 72/275 (26%), Positives = 121/275 (44%), Gaps = 33/275 (12%) Query: 11 MNYEDFQHILFEHRDDGVLLATLNRPEVMNATNARLHWELTKLWQVVKDDAATKVVVITG 70 M+YE+ Q E V L L+RP+ +NA + L EL + ++ITG Sbjct: 1 MSYENIQVETHEK----VGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITG 56 Query: 71 AGDRAFSAGGDLQWVEEMVGNPQKIATVHQEASDLVYNMLNCDKPIVSAINGTAVGAGLA 130 + ++AF+AG D + M G + + + KP+++A+ G A+G G Sbjct: 57 S-EKAFAAGAD---ISAMAGYDYMDVFKGEFITRNWETLRRIRKPVIAAVAGYALGGGCE 112 Query: 131 VALLADVSVIAEDARITDGHVKLGVAAGDHAAIVWPLLCGMAKAKYYLMTADFIDGKEAE 190 +A++ D + A++A+ +KLG+ G P AKA +T +D +EAE Sbjct: 113 LAMMCDFIIAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAE 172 Query: 191 RLGLVTFCEPPSEVL-------------PKALAVAAKLARGSQTAIRATKMSLNNWMRMA 237 R GLV+ ++L P+ +A+ K + RA + +L+ M+ Sbjct: 173 RAGLVSRIVAADKLLEEAMAAAIIIAEMPRQVAMMVK-----DSVNRAYETTLSEGMKYE 227 Query: 238 GPIFDNALAQEMLNFLGEDAREGLAAVRSKRQADF 272 +F + A ED +EG+ A KRQ F Sbjct: 228 RALFYSCFAT-------EDQKEGMKAFLEKRQPVF 255 >gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 75.9 bits (185), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 65/257 (25%), Positives = 113/257 (43%), Gaps = 8/257 (3%) Query: 19 ILFEHRDDGVLLATLNRPEVMNATNARLHWELTKLWQVVKDDAATKVVVITGAGDRAFSA 78 IL G+ TLNRP+ NA + + L +K D+ + V++ G +AF A Sbjct: 8 ILEREDQQGITTLTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAANG-KAFCA 66 Query: 79 GGDLQWVEEMVGNPQKI--ATVHQEASDLVYNMLNCDKPIVSAINGTAVGAGLAVALLAD 136 G DL+ +M PQ+ + S L+ ++ P+++ + G A AG + D Sbjct: 67 GHDLR---QMQATPQQTYYEALFHRCSHLMQSLRELPVPVIAKVQGLATAAGCQLVATCD 123 Query: 137 VSVIAEDARITDGHVKLGVAAGDHAAIVWPLLCGMAKAKYYLMTADFIDGKEAERLGLVT 196 +++ AE AR + +G+ A + L + +A L+T FID A GL+ Sbjct: 124 LAIAAESARFAVSGINVGLFCSTPAVALTRNL-PIKRAFEMLVTGKFIDAATAAAWGLIN 182 Query: 197 FCEPPSEVLPKALAVAAKLARGSQTAIRATKMSLNNWMRMAGP-IFDNALAQEMLNFLGE 255 P E+ A+A ++ +I K + MA P + +A N + Sbjct: 183 QAVPEQELDSATQALAREICSKPAISIARGKAMVYRQQMMALPDAYAHAAEVMACNIMDP 242 Query: 256 DAREGLAAVRSKRQADF 272 +A+EG+ A KR+ ++ Sbjct: 243 EAQEGITAFLDKRKPNW 259 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 754,473 Number of extensions: 25940 Number of successful extensions: 105 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 96 Number of HSP's successfully gapped: 8 Length of query: 278 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 194 Effective length of database: 1,222,130 Effective search space: 237093220 Effective search space used: 237093220 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)