BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0017.p (647 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [H... 280 3e-76 gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspir... 279 4e-76 gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 207 3e-54 gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [H... 139 9e-34 gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligas... 112 8e-26 >gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 660 Score = 280 bits (715), Expect = 3e-76, Method: Compositional matrix adjust. Identities = 191/607 (31%), Positives = 309/607 (50%), Gaps = 23/607 (3%) Query: 54 WDVKPTAYVDLARGPEFPRWFPGGRLNWVNTVLSYARRADSSGRLALVAESEDGSTRQLT 113 W T +D + P F +WF G++N L ++ + A++ ES+DG +++ Sbjct: 57 WHKPFTQTLDESNAP-FYKWFADGQVNVSYNCLDVNLENGNADKTAVIFESDDGKVTRVS 115 Query: 114 YAQLAQQVSDFAAGLGVLGVERGDRVGLMMENGVEATVSALAIAYMGAIMVPLFSGFGVD 173 Y +L Q+V FA GL LG+++GDRV + M VE + A A +GA +F GF Sbjct: 116 YRELHQKVCQFANGLKSLGIKKGDRVVIYMPMSVEGVAAMQACARIGATHSVVFGGFSAK 175 Query: 174 AIISRFSAAEVSFVISTTGFARRTKRVNVESPMREAW---ARTPAVRGIIWKRGPGEQGG 230 ++ R A V++ R K++ ++S + EA +++KR G Sbjct: 176 SLHERVIDAGAVAVLTADYQVRGGKQLPLKSIVDEALDMGGCDTLKHVVVYKRA----GA 231 Query: 231 KANG-DARDHDWNDVLELGAEKPAPAASMDANDPFMVIYTSGTTGKPKGVVHTHGSFPLK 289 + N + RD +DV+ ++ P +DA P ++YTSG+TGKPKGV H + L Sbjct: 232 EINWVEGRDLWLSDVVANQSDTCEPEW-VDAEHPLFILYTSGSTGKPKGVQHASAGYLLW 290 Query: 290 IAHDSAVHFNVREGDVYCWPADMGWIAG-SLVLSSALLRGATLVCYDGAPDYPDWSRMSR 348 F+++ DVY AD+GWI G + + L GAT + ++G P YP+ R Sbjct: 291 AVLTMKWTFDIKPDDVYWCTADIGWITGHTYIAYGPLAVGATQIVFEGVPTYPNAGRFWD 350 Query: 349 LVERHGVTHFGSAPTLIRGM-----AGNEAMALQGDVSSIRMLITAGEGIDPEHFSWFQQ 403 +V RH T F +APT IR + A + Q D+SS+R+L + GE I+PE + W+ + Sbjct: 351 MVARHKATIFYTAPTAIRSLIKAADADEKVHPKQYDLSSLRILGSVGEPINPEAWMWYYK 410 Query: 404 TFGRGERPLINYTGGTEVSGALLSSVV-LRPIPPSGFNTTSPGVNVAVVDASGAPVIDVV 462 G P+++ TE G ++S + P P PG+ A+VD +G + + Sbjct: 411 NVGHENCPIVDTFWQTETGGHMISPLPGATPQVPGSCTLPLPGITAAIVDETGNDIPNGN 470 Query: 463 GE-LAVREPFVGMTQSFWKDDQRYLETYWRTTPG--IWVHGDLALHRSSDGNYFMMGRSD 519 G L V+ P+ M ++ W D +R+ ++Y+ G I++ GD A+ G + + GR D Sbjct: 471 GGILVVKRPWPSMIRTIWNDPERFKKSYFPEELGGKIYLAGDGAVRNKETGYFTITGRID 530 Query: 520 DTLKVAGKRLGPAEVEEVVLELDSIAEAAAIGVADADKGQRLVIFVVTLAGVDTSEEA-- 577 D L V+G R+G E+E ++ +AEAA +G D G+ + FVV T +EA Sbjct: 531 DVLNVSGHRMGTMEIESALVANSIVAEAAVVGKPDETTGESICAFVVLKRPRPTGDEAKQ 590 Query: 578 LLARVSQHVDVRLGRPFRPSAVHVVKQLPKTRTTKIMRRVIRSVYTG-APPGDLSSLDNP 636 + + V +G +P + LPKTR+ KIMRR++R + G A D+S+L+NP Sbjct: 591 IAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRSGKIMRRLLRVLAKGEAITQDVSTLENP 650 Query: 637 AALDEIR 643 A LD+++ Sbjct: 651 AILDQLK 657 >gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 628 Score = 279 bits (714), Expect = 4e-76, Method: Compositional matrix adjust. Identities = 205/636 (32%), Positives = 322/636 (50%), Gaps = 29/636 (4%) Query: 27 NYDELLQLAEADPARYWNVVQGYCGIVWDVKPTAYVDLARGPEFPRWFPGGRLNWVNTVL 86 Y++ + + P +W Q I W + +D +R P F RWF G+ N + + Sbjct: 2 QYEQFYRQSVEQPEAFW--AQEAQRIHWQQPFSRTLDYSR-PPFARWFIDGQTNLCHNAV 58 Query: 87 S--YARRADSSGRLALVAESEDGSTRQLTYAQLAQQVSDFAAGLGVLGVERGDRVGLMME 144 RA + +A+ +E S R TYA+L Q+V AA + LGV+RGDRV + M Sbjct: 59 DRHLPERAQQAALIAI--STETNSERVYTYAELQQEVMAMAASMQALGVQRGDRVLIYMP 116 Query: 145 NGVEATVSALAIAYMGAIMVPLFSGFGVDAIISRFSAAEVSFVISTTGFARRTKRVNVES 204 EA + LA A +GA+ +F GF +++ SR A+ ++S +R K + + Sbjct: 117 MIAEAVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQPRLIVSADAGSRGGKVIPYKG 176 Query: 205 PMREA--WARTPAVRGIIWKRGPGEQGGKANGDARDHDWNDVLELGAEKPAPAASMDAND 262 + EA A+ ++ RG A RD D+ + + P +++N Sbjct: 177 LLDEAIAMAQHRPQHVLLVDRGLAPMARTAQ---RDVDYAPLRTQHLGQQVPVTWLESNA 233 Query: 263 PFMVIYTSGTTGKPKGVVHTHGSFPLKIAHDSAVHFNVREGDVYCWPADMGWIAG-SLVL 321 ++YTSGTTGKPKGV G + + +A F + G+ Y +D+GW+ G S ++ Sbjct: 234 SSYILYTSGTTGKPKGVQRDVGGYAVALASSMQHIFCGQPGETYFCTSDIGWVVGHSYIV 293 Query: 322 SSALLRGATLVCYDGAPDYPDWSRMSRLVERHGVTHFGSAPTLIRGMAGNEAMALQG-DV 380 L+ G V Y+G P PD +VE++ VT SAPT IR + ++ D Sbjct: 294 YGPLIAGMATVLYEGLPIRPDAGIWWSIVEKYKVTRMFSAPTAIRVLKKQPPECMERYDT 353 Query: 381 SSIRMLITAGEGIDPEHFSWFQQTFGRGERPLINYTGGTEVSGALLS---SVVLRPIPPS 437 SS++ L AGE +D SW G + P+I+ TE ++S + +P Sbjct: 354 SSLKALYLAGEPLDETTSSWIS---GALKVPVIDNYWQTESGWPIISIAKGIDDKPTRLG 410 Query: 438 GFNTTSPGVNVAVVD-ASGAPV-IDVVGELAVREPFV-GMTQSFWKDDQRYLETYWRTTP 494 PG +A++D A+G P D G +A+ P G Q+ + DD+R++ TYWR+ P Sbjct: 411 SPGVPMPGYRLAILDEATGEPCGPDQKGVVAIEGPLPPGCMQTVYGDDERFVNTYWRSLP 470 Query: 495 -GIWVHGDLALHRSSDGNYFMMGRSDDTLKVAGKRLGPAEVEEVVLELDSIAEAAAIGVA 553 ++ D + R DG YF++GR+DD + VAG RLG E+EE + +++E A +GV Sbjct: 471 REVYSTFDWGI-RDKDGYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVVGVE 529 Query: 554 DADKGQRLVIFVVTLAG--VDTSEE--ALLARVSQHVDVRLGRPFRPSAVHVVKQLPKTR 609 D KGQ F + + T E+ AL A + VD ++G RP+ V V LPKTR Sbjct: 530 DKLKGQVAFAFAIPKQAYTIATPEQRKALEAEIMAVVDKQIGAVGRPARVFFVSGLPKTR 589 Query: 610 TTKIMRRVIRSVYTGAPPGDLSSLDNPAALDEIRAV 645 + K++RR I+S+ G PGDL ++++PAAL+++RAV Sbjct: 590 SGKLLRRAIQSICEGRDPGDLPTIEDPAALEQVRAV 625 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 207 bits (526), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 169/540 (31%), Positives = 256/540 (47%), Gaps = 41/540 (7%) Query: 90 RRADSSGRLALVAESEDGSTRQLTYAQLAQQVSDFAAGLGVLGVERGDRVGLMMENGVEA 149 R AD SGR A++ E DGS +Y QL FA L GV RGDR+G+ + +E Sbjct: 41 RWADGSGRTAIICEDADGSVTSYSYDQLKILSDRFANALRAAGVGRGDRIGIYLSQRIET 100 Query: 150 TVSALAIAYMGAIMVPLFSGFGVDAIISRF-SAAEVSFVISTTGFARRTKRVNVESPMRE 208 ++ LA +GAI VPLF FG DAI R ++ V+ V +G + T Sbjct: 101 VITHLAAYKLGAITVPLFYLFGPDAIAYRLDNSGAVALVTDASGMEKATLA--------- 151 Query: 209 AWARTPAVRGIIWKRGPGEQGGKANGDARDHDWNDVLELGAEKPAPAASMDANDPFMVIY 268 PA+R + + D+ D L + P ++ A+DP M+IY Sbjct: 152 --GELPALRLVFCVESSDLVLPQTT------DFWDRLHAAPAELDPVLTL-ADDPAMIIY 202 Query: 269 TSGTTGKPKGVVHTH----GSFP-LKIAHDSAVHFNVREGDVYCWPADMGWIAGSL-VLS 322 TSGTTGK KG +H H G P ++++HDS +EGD + PAD WI G L VL Sbjct: 203 TSGTTGKAKGALHAHRVLLGHLPGVEVSHDSFP----QEGDRFWTPADWAWIGGLLDVLL 258 Query: 323 SALLRGATLVCYDGAPDYPDWSRMSRLVERHGVTHFGSAPTLIRGMAGNEAMALQGDVSS 382 +L G +V + D + + L+ RH + + PT ++ + G + Q D S Sbjct: 259 PSLYHGVAVVARR--LEKFDAAEVFGLLARHQIRNVFFPPTALKMLRGAATVRAQADFS- 315 Query: 383 IRMLITAGEGIDPEHFSWFQQTFGRGERPLINYTGGTEVSGALLSSVVLRPIPPSGFNTT 442 +R + + GE + + +W ++ G + + G TE + + SS P Sbjct: 316 LRSVASGGETLGDDLIAWGREALGV---TINEFYGQTECNLVVSSSSHCYPSVSGSMGRA 372 Query: 443 SPGVNVAVVDASGAPVID-VVGELAVREPFVGMTQSFWKDDQRYLETYWRTTPGIWVHGD 501 PG V +VD G + VG +A+R P M +W++++ E + + GD Sbjct: 373 VPGHVVQIVDEQGQVLPHGTVGNIAIRAPDPVMFLRYWRNEEATREKF---AGDFLLTGD 429 Query: 502 LALHRSSDGNYFMMGRSDDTLKVAGKRLGPAEVEEVVLELDSIAEAAAIGVADADKGQRL 561 L G +GR+DD + AG R+GPA +EE ++ ++ AA +GV DA + + + Sbjct: 430 LG-SMDEQGYIRYLGRNDDVITSAGYRIGPAAIEECLMRHPAVRIAAVVGVKDALRTEVV 488 Query: 562 VIFVVTLAGVDTSEEALLARVSQHVDVRLGRPFRPSAVHVVKQLPKTRTTKIMRRVIRSV 621 FVV GV T +EAL A + QHV +L P + V LP T T KIMR+ ++ + Sbjct: 489 KAFVVLKDGV-TPDEALKAELQQHVRAQLAAHEYPRLISFVAALPTTATGKIMRKTLKEM 547 >gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 139 bits (349), Expect = 9e-34, Method: Compositional matrix adjust. Identities = 151/569 (26%), Positives = 238/569 (41%), Gaps = 71/569 (12%) Query: 74 FPGGRLNWVNTVLSYARRADSSGRLALVAESEDGSTRQLTYAQLAQQVSDFAAGLGVLGV 133 F G +N + R +AL S G +Q ++AQ+ + A L G+ Sbjct: 29 FQGDFETGINACVECCDRHVKGDNIALEFVSLHGEHQQYSFAQVRSMAARVANLLRQQGI 88 Query: 134 ERGDRVGLMMENGVEATVSALAIAYMGAIMVPLFSGFGVDAIISRFSAAEVSFVISTTGF 193 + G V M+ E + L GA+ PLF+ FG AI R + + S ++ T Sbjct: 89 QPGQIVAGMLPRTPELLATVLGTLRAGAVYQPLFTAFGPKAIEHRLALSGASLIV--TNV 146 Query: 194 ARRTKRVNVESPMREAWARTPAVRGIIWKRGP---GEQGGKANGDARDHDWNDVLELGAE 250 A R K + A P V + P G+ +A DA+ D+ VL GA Sbjct: 147 ANRDKLDEI--------ANCPQVCTVRESNDPLRAGDIDFRAAVDAQSDDFAPVLRSGA- 197 Query: 251 KPAPAASMDANDPFMVIYTSGTTGKPKGVVHTHGSFPLKIAHDSAVHFNVREG------D 304 D +++ TSGTTG KGV P+ ++ A +RE D Sbjct: 198 -----------DLMLMMSTSGTTGAAKGV-------PVPLSALQAFSVYMREAVGLLPQD 239 Query: 305 VYCWPADMGW-------IAGSLVLSSALLRGATLVCYDGAPDYPDWSRMSRLVERHGVTH 357 + AD GW I G L + + G + R G+T Sbjct: 240 KFWNMADPGWAYGLYYAIIGPLAIGHGITFNEAAFTVSGT---------YETIRRLGITS 290 Query: 358 FGSAPTLIRGMAGNEAMALQGDVSSIRMLITAGEGIDPEHFSWFQQTFGRGERPLINYTG 417 APT R M A +R + +AGE ++ E WF + P+ ++ G Sbjct: 291 LAGAPTAYRMMMAAGEEAAASVKGRLRAVSSAGEPLNAEVVRWFASAL---DVPIHDHYG 347 Query: 418 GTEVSGALLSSVVLR-PIPPSGFNTTSPGVNVAVVDASGAPV-IDVVGELAV---REPFV 472 TE+ + + LR + P PG V V+D + + + GELA+ R P Sbjct: 348 QTELGMVVNNHHALRHEVVPGSAGFAMPGYRVVVLDENHQELGANQPGELAIDIARSPLY 407 Query: 473 GMTQSFWKDDQRYLETYWRTTPGIWVHGDLALHRSSDGNYFMMGRSDDTLKVAGKRLGPA 532 + +WK D + + +T GD + +G+ +GRSDD + AG R+GP Sbjct: 408 WFS-GYWKQDTPAIAHGYYST------GD-NVELEPNGSISFIGRSDDVITSAGYRIGPF 459 Query: 533 EVEEVVLELDSIAEAAAIGVADADKGQRLVIFVVTLAGVDTSEEALLARVSQHVDVRLGR 592 +VE +LE ++A+ A IG+ D ++ + + FVV L+ + +AL +++QHV RL Sbjct: 460 DVESALLEHPAVADVAVIGLPDPERTEIVKAFVV-LSDQFKASDALREQLAQHVKRRLSA 518 Query: 593 PFRPSAVHVVKQLPKTRTTKIMRRVIRSV 621 P A+ + LPKT + K+ R V+R + Sbjct: 519 HAYPRAIEFLDALPKTPSGKLQRFVLRKM 547 >gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 112 bits (281), Expect = 8e-26, Method: Compositional matrix adjust. Identities = 138/559 (24%), Positives = 243/559 (43%), Gaps = 77/559 (13%) Query: 89 ARRADSSGRLALVAESEDGSTRQLTYAQLAQQVSDFAAGLGVLGVERGDRVGLMMENGVE 148 ARR L L + DGS R + + ++ +V A L GV++GD++ + N + Sbjct: 40 ARRFPDEPGLILAQGAADGSDRLIPWREINARVDALAHALTRRGVKKGDKMLVHSRNNQQ 99 Query: 149 ATVSALAIAYMGAIMVPLFSGFGVD----AIISRFSAAEVSFVISTTGFARRTKRVNVES 204 SA A MG + VP + F + A + + S A V ++ GFA+ V Sbjct: 100 IFESAWAAFKMGVVWVP--TNFRITPPEAAYLGQSSGACV--MLYDRGFAQYVDAVK--- 152 Query: 205 PMREAWARTPAVRGIIWKRGPGEQGGKANGDARDHDWNDVLELGAEKPAPAASMDANDPF 264 A +PA+ ++ A+ A + D+ ++ G+ A +D +DP Sbjct: 153 ------AASPALEHVV---------ALADARAGELDYEALVREGSGAAFREAEVDYDDPL 197 Query: 265 MVIYTSGTTGKPKGVVHTHGSFPLKIAH---DSAVHFNVREGDVYCWPADMGWIAGSLV- 320 YTSGTTG PK + +HG + + D + + P G +++ Sbjct: 198 WFFYTSGTTGHPKAGMLSHGQMAFVVTNHLADLMPGLTHQSRSLVVAPLSHGAGVHAVIN 257 Query: 321 ----LSSALLRGATLVCYDGAPDYPDWSRMSRLVERHGVTHFGSAPTLIRGMAGNEAMAL 376 +S LL LV + W +LVE++ V + + PT+++ + +E++ Sbjct: 258 TARGAASILLSSERLVVEEA------W----QLVEKYRVDNLFTVPTIVKILVEDESVD- 306 Query: 377 QGDVSSIRMLITAGEGIDPEHFSWFQQTFGRGERPLINYTGGTEVSGALLSSVVLRPI-- 434 + D SS++ +I AG + + + G+ L+ Y G EV+G + L P Sbjct: 307 RYDHSSLKHVIYAGAPMYRADQVYALKKLGK---VLVQYYGLGEVTGNI---TFLPPYLH 360 Query: 435 ------PPSGFNTTS---PGVNVAVVDASGAPVIDV-VGELAVREP--FVGMTQSFWKDD 482 P + T G+ +A+++ G + GE+ VR P F+G + + Sbjct: 361 FEDDAHPQARVGTCGMPRTGMQIAILNDDGQELAPFETGEICVRGPAVFMGYHNNPEANA 420 Query: 483 QRYLETYWRTTPGIWVHGDLALHRSSDGNYFMMGRSDDTLKVAGKRLGPAEVEEVVLELD 542 + + ++ T GDL H DG ++ GRS D G + P E+EE +L Sbjct: 421 KAFKHDWFHT-------GDLG-HVDQDGFLYITGRSSDMYISGGSNVYPREIEEALLTHP 472 Query: 543 SIAEAAAIGVADADKGQRLVIFVVTLAGVDTSEEALLARVSQHVDVRLGRPFRPSAVHVV 602 +++E A +GV D G+ + +V AG EALLA H++ R+G+ P Sbjct: 473 AVSEVAVLGVPDPKWGESGLAVIVCKAGQQVDGEALLA----HLESRIGKYKWPRRFIYW 528 Query: 603 KQLPKTRTTKIMRRVIRSV 621 +PK+ KI+++ I+++ Sbjct: 529 DSMPKSGYGKIVKKQIKAL 547 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,244,791 Number of extensions: 103152 Number of successful extensions: 370 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 334 Number of HSP's successfully gapped: 14 Length of query: 647 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 556 Effective length of database: 1,188,985 Effective search space: 661075660 Effective search space used: 661075660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)