BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0015.p (300 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogen... 224 7e-60 gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenas... 158 5e-40 gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogen... 108 5e-25 gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum s... 69 7e-13 >gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 297 Score = 224 bits (571), Expect = 7e-60, Method: Compositional matrix adjust. Identities = 123/289 (42%), Positives = 181/289 (62%), Gaps = 2/289 (0%) Query: 11 IGFIGLGQMGAPMVRSLLRAGRKVHVYDINPQAVAEALS-HGAIAANSIASLASQSDLIV 69 I FIGLG MG PM ++L++AG V +D+ +V + + G A+S+A++A ++D+++ Sbjct: 5 IVFIGLGNMGLPMAQNLVKAGFSVSGHDLVKASVDKLVEVGGKTEADSMAAVA-KADVVI 63 Query: 70 TMLPSPSHVRAAFLDDDGILAHAAKGSLVVDCSTIDPETAKQLHQTSRLRGIDMADAPVS 129 TMLP+ HV + +L + G+LA A G+L++DCSTI PE A+++ ++ RG +M DAPVS Sbjct: 64 TMLPASRHVESLYLGEAGVLASAKPGTLLIDCSTIAPEAARKVAAAAKARGFEMLDAPVS 123 Query: 130 GGVLGAAAATLTLLVGAVPALFERIREVLAPVGSEFIRCGEPGAGQVVKIANNLLAAISM 189 GG GA A TLT +VG FE + L +G GE G+GQ VK+ NN+L I M Sbjct: 124 GGTNGATAGTLTFMVGGSKEAFEAAQPYLQKMGKAIYHAGESGSGQTVKVCNNMLLGILM 183 Query: 190 VATSEAMTLGVKLGADPVLLANAINHSTGRCWASEHYNPWPGVCPDAPASHDYVGGASTG 249 + TSEA+ LG+ G DP +L+ ++ S+GR W E YNP PGV APAS Y GG Sbjct: 184 IGTSEAIRLGMANGMDPKVLSEIMSKSSGRNWTLEVYNPCPGVMETAPASKGYAGGFGVD 243 Query: 250 ILIKDLSLALGEAKGVTMPLFLGAIAQQVYQLSAAQGNGKKDFASVVNL 298 +++KDL LA+ A + LGA A+ +Y + + G GK DF+S+ N+ Sbjct: 244 LMLKDLGLAVENALATNCAIPLGATARNLYDIHSMNGAGKLDFSSIFNM 292 >gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 298 Score = 158 bits (400), Expect = 5e-40, Method: Compositional matrix adjust. Identities = 95/290 (32%), Positives = 160/290 (55%), Gaps = 8/290 (2%) Query: 11 IGFIGLGQMGAPMVRSLLRAGRKVHVYDINPQAVAEALSHGAIAANSIASLASQSDLIVT 70 +G IGLG MG + SLLRAG VH D+ + + + + G +A + A LA + D+++T Sbjct: 5 VGVIGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVACANPAELAGKVDVVIT 64 Query: 71 MLPSPSHVRAAFLDDDGILAHAAKGSLVVDCSTIDPETAKQLHQTSRLRGIDMADAPVSG 130 ++ + + D+G ++ GS+V+ +T+ P+ A +L + +G+ + DAPVSG Sbjct: 65 LVVNAAQTETVLFGDNGAVSAMKPGSVVISSATVAPDFAIELGKRLVEKGLLLLDAPVSG 124 Query: 131 GVLGAAAATLTLLVGAVPALFERIREVLAPVGSEFIRCGEP-GAGQVVKIANNLLAAISM 189 G AA+ +T++ ++ +I +VLA + + R G+ G G VKI N LLA + + Sbjct: 125 GAARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLAGVHI 184 Query: 190 VATSEAMTLGVKLGADPVLLANAINHSTGRCWASEHYNPWPGVCPDAPASHDYVGGASTG 249 A++EAM LG++ G +P L I HS G W E N P + S DY ++ Sbjct: 185 AASAEAMALGLREGVNPDALYEVITHSAGNSWMFE--NRVPHIL-----SGDYTPLSAVD 237 Query: 250 ILIKDLSLALGEAKGVTMPLFLGAIAQQVYQLSAAQGNGKKDFASVVNLY 299 I +KDL L L A+ PL L A A Q++ +++ G+G +D ++V+ ++ Sbjct: 238 IFVKDLGLVLDTARRSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIKIF 287 >gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 288 Score = 108 bits (271), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 91/293 (31%), Positives = 139/293 (47%), Gaps = 18/293 (6%) Query: 11 IGFIGLGQMGAPMVRSLLRAGRKVHVYDINPQAVAEALSHGAIAANSIASLASQSDLIVT 70 IG IGLG MG M SL R G +V YD G +SIA+LA +++V Sbjct: 4 IGVIGLGNMGRGMAFSLQRGGYEVLGYDAQESTRTALAQQGLHCVDSIAALAPAVEMVVL 63 Query: 71 MLPSPSHVRAAFLDDDGILAHAAKGSLVVDCSTIDPETAKQLHQTSRLRGIDMADAPVSG 130 LP+ V + G+L H A G ++VD +T DP + ++L + G+++ DAPVSG Sbjct: 64 SLPTSDIVEQVVEGEGGLLQHGAPGLVIVDTTTADPNSTRRLAARLQAAGMELVDAPVSG 123 Query: 131 GVLGAAAATLTLLVGAVPALFERIREVLAPVGSEFIRCGEPGAGQVVKIANNLLAAISMV 190 G GAA AT+ +++G RI VLA + ++ + G GAG V K+ NNL+ ++ Sbjct: 124 GPKGAATATMGMVIGGSAETVARIEPVLAVMSAKRVHVGPVGAGHVAKLLNNLVTGTHLL 183 Query: 191 ATSEAMTLGVKLGADPVLLANAINHSTGRCWASEHYNP-WPGVCPDAPASHDYVGGASTG 249 EA + G D + + + E + P W + +A + G + Sbjct: 184 IAGEAARIAAAAGLDARQIFQGLAAGSANSKVIEVFYPTW--IFNEA-----FDSGFTMK 236 Query: 250 ILIKDLSLALG--EAKGVTMPLFLGAIAQQVYQLSAAQGNG---KKDFASVVN 297 ++ KDL LA+ E T PL A++ +L A G +DF +VN Sbjct: 237 LMRKDLRLAMNLIEETRSTTPL-----AREAGRLWARSVEGLADAEDFNRIVN 284 >gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 292 Score = 68.6 bits (166), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 83/298 (27%), Positives = 132/298 (44%), Gaps = 32/298 (10%) Query: 11 IGFIGLGQMGAPMVRSLLRAGRKVHVYDINPQAVAEALSHGAIAANSIASLASQSDLIVT 70 I F+GLG MG PM +L+RAG V ++ +P V + GA AA + A A+ +D++++ Sbjct: 3 IAFLGLGTMGFPMAANLVRAGYAVRAWNRSPAPVERLAALGAQAAATPAEAAADADVLIS 62 Query: 71 MLPSPSHVRAAFLDDDGILAHAAK---------GSLVVDCSTIDPETAKQLHQTSRLRGI 121 ML DDD G++ V+ +TI A +L + RG+ Sbjct: 63 MLA----------DDDATETSLLDAGALAALRPGAIHVNMATISVALALRLAGLHQARGV 112 Query: 122 DMADAPVSGGVLGAAAATLTLLVGAVPALFERIREVLAPVGSEFIRCG-EPGAGQVVKIA 180 APV G V A A L +L ++ +L +G + R G +P K+A Sbjct: 113 AYVAAPVLGRVNVAEAGQLNILAAGEEQALAAVQPLLDVLGQKTWRLGRQPEQANAAKLA 172 Query: 181 NNLLAAISMVATSEAMTLGVKLGADPVLLANAINHSTGRCWASEHYNPWPGVCPD---AP 237 N + A ++ A SEA+ L G D A I +T +A+ Y + D P Sbjct: 173 MNFMIASAIGAMSEAVALVQGYGLDK---AGFIELATSTAFAAPVYKGYGQAIADDRFEP 229 Query: 238 ASHDYVGGASTGILIKDLSLALGEAKGVTMPLFLGAIAQQVYQLSAAQGNGKKDFASV 295 A G + +KD+ LAL + +PL L + + ++ A G G D+A++ Sbjct: 230 A------GFKLALGLKDVRLALEAGEQAHVPLSLASALRDLHIDGLAHGEGHLDWAAL 281 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 824,977 Number of extensions: 30786 Number of successful extensions: 144 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 140 Number of HSP's successfully gapped: 4 Length of query: 300 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 215 Effective length of database: 1,217,395 Effective search space: 261739925 Effective search space used: 261739925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)