BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0010.p (503 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbasp... 117 2e-27 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 95 1e-20 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 91 2e-19 gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbasp... 78 1e-15 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 72 7e-14 >gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 117 bits (293), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 76/257 (29%), Positives = 129/257 (50%), Gaps = 14/257 (5%) Query: 116 TFPRDADWDAMSEPQFRALVRGFFRQNYPDHLRHLSRRLHWNEIGEWYLTLSRQGWIAPA 175 ++P +D S R VR + P++ R + + E ++ L++ GW+A Sbjct: 2 SYPTASD----SHADLRDAVRDLCKTFPPEYWRKIDEARGYPE--QFVDALTKAGWLAAL 55 Query: 176 WPKEHGGMGLSPDKHLAFIEEQEKYGVARMIDAGLTMTGPLLIRHGTPAQQQAYLPKIIS 235 P+E+GG GL + +EE + G G L+RHG+P Q++ YLP+I S Sbjct: 56 IPQEYGGSGLGLTEASVIMEEINRTGGNSGACHGQMYNMGTLLRHGSPEQKERYLPRIAS 115 Query: 236 GEHIWSQGYSEPNAGSDLASLRTEAVLDQDGNNFIVNGQKTWTTMAQDSTHIFMLVRTS- 294 GE + Q + + + R + V + G+ +++NGQK W + Q S + +L RT+ Sbjct: 116 GE-LRLQSMAVTEPTTGTDTTRIKTVAVKKGDRYVINGQKVWISRVQHSDLMILLARTTP 174 Query: 295 --RSGKPQAGISFLLADIHSP---GITVRPIRDLVGHEHFCEVFFDNVKVPKDNLVGEIN 349 + + G+S L D+ G+ VRPI ++V HE E+FFDN+++P +NL+GE Sbjct: 175 LEQVSRKSEGMSIFLVDLKEAIGHGMEVRPILNMVNHET-NELFFDNLEIPAENLIGEEG 233 Query: 350 QGWSLAKALLGFERIFL 366 +G+ L ER + Sbjct: 234 RGFRYILDGLNAERTLI 250 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 95.1 bits (235), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 62/219 (28%), Positives = 112/219 (51%), Gaps = 15/219 (6%) Query: 156 WNEIGEWYLTLSRQGWIAPAWPKEHGGMGLSPDKHLAFIEEQEKYGVARMIDAGL--TMT 213 W ++G+ G + +E+GG GL H+ +EE + + + G + Sbjct: 48 WKKLGDL-------GVLGITVSEEYGGAGLGYLAHIIAMEEISRASASVGLSYGAHSNLC 100 Query: 214 GPLLIRHGTPAQQQAYLPKIISGEHIWSQGYSEPNAGSDLASLRTEAVLDQDGNNFIVNG 273 + R+G Q++ YLP++ISG+ I + SEPNAGSD+ S++ A D+ G+ +++NG Sbjct: 101 VNQIKRNGNEEQKRKYLPRLISGDFIGALAMSEPNAGSDVVSMKLRA--DKKGDRYVLNG 158 Query: 274 QKTWTTMAQDSTHIFMLVRTSRSGKPQAGISFLLADIHSPGITVRPIRDLVGHE--HFCE 331 K W T D+ + + +T + G++ L + G +V D +G H E Sbjct: 159 SKMWITNGPDADVLVVYAKTDLEAGAR-GMTAFLVEKGYKGFSVAQKLDKLGMRGSHTGE 217 Query: 332 VFFDNVKVPKDNLVGEINQGWSLAKALLGFERIFL-GNP 369 + F + +VP++N++G + +G ++ + L FER L G P Sbjct: 218 LVFQDCEVPEENVLGGVGRGVNVLMSGLDFERSVLSGGP 256 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 91.3 bits (225), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 83/348 (23%), Positives = 155/348 (44%), Gaps = 30/348 (8%) Query: 166 LSRQGWIAPAWPKEHGGMGLSPDKHLAFIEE--QEKYGVARMIDAGLTMTGPLLIRHGTP 223 L+ G + P++ GG G+ + +EE + ++ ++ + ++G+P Sbjct: 43 LAELGAMGMCVPEQWGGAGMDYMSLVLALEEIAAGDGATSTIVSVQNSLACGITEKYGSP 102 Query: 224 AQQQAYLPKIISGEHIWSQGYSEPNAGSDLASLRTEAVLDQDGNNFIVNGQKTWTTMAQD 283 Q++ +L + GE + +EP+ GSD A++RT A ++DG++FI+NG K + T + Sbjct: 103 TQKEQWLKPLARGEMLGCFCLTEPHTGSDAAAIRTRA--ERDGDDFILNGTKQFITSGKH 160 Query: 284 STHIFMLVRTSRSGKPQAGISFLLADIHSPGITVRPIRDLVGH--EHFCEVFFDNVKVPK 341 + + T RS + GIS L +PG V + +G ++ DN +VP Sbjct: 161 AGVAIVFAVTDRSAGKK-GISCFLVPCDTPGFVVGRSEEKMGQHASDTVQIMLDNCRVPA 219 Query: 342 DNLVGEINQGWSLAKALLGFERIFLGNPRQSQNALAKLHALAAAQA-------LFEDPLF 394 L+G+ +G+ +A + L RI + A A++ A+ + E Sbjct: 220 TALLGKEGEGYRIALSNLEAGRIGIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAV 279 Query: 395 NSKYARL--LLDVADLSALYAKYADMVKRGESLPPSVSILKIWATETYRRIG--LLLYEA 450 N + A + LLD A L + + A + +G S+ K++A+E +I + Sbjct: 280 NFRLADMNTLLDAARL--MVWRAAQLKDQGRPCLKEASMAKMFASEAAEKIASDAIQIHG 337 Query: 451 GQEYGGNHPIEELGGDFDNLLTPLINSASATIYGGTNEIQRNILAKTV 498 G Y + P+E + D IY G N+IQR ++ + + Sbjct: 338 GVGYTSDFPVERIYRDVR----------ICQIYEGANDIQRLVIGRAI 375 >gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 415 Score = 78.2 bits (191), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 56/222 (25%), Positives = 106/222 (47%), Gaps = 17/222 (7%) Query: 162 WYLTLSRQGWIAPAWPKEHGGMGLSPDKHLAFIEEQEKYGV---ARMIDAGLTMTGPLLI 218 +Y ++ G+I P+ GG LS EE V + ++ GL + ++ Sbjct: 49 FYQAMADAGFIKALLPESFGGTALSTLDFALAAEELTAVDVNVPSALLGTGLGLHA--VV 106 Query: 219 RHGTPAQQQAYLPKIIS-GEHIWSQGYSEPNAGSDL----ASLRTEAVLDQDGNNFIVNG 273 HGTP Q+Q +LP I G + + ++E G++ S+ + +G+ +++NG Sbjct: 107 HHGTPEQKQRFLPDFIEDGSRLAALAFTEVTGGANYDSTDPSVGVQTFARLEGDEWVING 166 Query: 274 QKTWTTMAQ----DSTHIF-MLVRTSRSGKPQAGISFLLADIHSPGITVRPIRDLVGHEH 328 K +TT H++ ++ RT + PQ ++ ++ ++PGI + + D +GH Sbjct: 167 AKHYTTNGTGWDGKGAHLYSVICRTDPALPPQQSLAVIMVPGNAPGIEIVGLLDTMGHRA 226 Query: 329 FC--EVFFDNVKVPKDNLVGEINQGWSLAKALLGFERIFLGN 368 V F+NV+VP N+VG+ G S+ +A + +G+ Sbjct: 227 TISPRVHFNNVRVPASNIVGKPGDGISMLEAAFSWTAALIGS 268 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 72.4 bits (176), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 59/200 (29%), Positives = 100/200 (50%), Gaps = 11/200 (5%) Query: 170 GWIAPAWPKEHGGMGLSPDKHLAFIEEQEKYGVARMIDAGLT---MTGPLLIRHGTPAQQ 226 G+ P+ GG+GLS EE + A LT M ++ G P+ + Sbjct: 49 GFCGLYTPEADGGLGLSRLDATVVFEELAR--ACTSTAAYLTIHNMVSWMIASWGQPSLK 106 Query: 227 QAYLPKIISGEHIWSQGYSEPNAGSDLASLRTEAVLDQDGNNFIVNGQKTWTTMAQDSTH 286 + ++ +G I S +EP +GSD ASL+T A L +DG ++++NG K + + A + Sbjct: 107 AQWCEQLAAGRKIGSYCLTEPGSGSDAASLKTTARL-EDG-HYVLNGSKAFISGAGSTDV 164 Query: 287 IFMLVRTSRSGKPQAGISFLLADIHSPGITVRPIRDLVG--HEHFCEVFFDNVKVPKDNL 344 + ++ RT G+ G+S ++ ++PG++ +G + + FDNVKVP D+L Sbjct: 165 LVVMART--GGEGARGVSAIVVPGNAPGVSYGKKESKMGWNSQPTRTISFDNVKVPADHL 222 Query: 345 VGEINQGWSLAKALLGFERI 364 +G+ QG+ A L RI Sbjct: 223 LGQEGQGFIFAMKGLDGGRI 242 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,625,999 Number of extensions: 71684 Number of successful extensions: 219 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 204 Number of HSP's successfully gapped: 7 Length of query: 503 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 414 Effective length of database: 1,198,455 Effective search space: 496160370 Effective search space used: 496160370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)