BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0004.p (386 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbasp... 634 0.0 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 184 1e-47 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 180 1e-46 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 167 1e-42 gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbasp... 114 1e-26 >gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 634 bits (1634), Expect = 0.0, Method: Compositional matrix adjust. Identities = 314/383 (81%), Positives = 347/383 (90%) Query: 4 APDQYQDIRDAVRDLCKEFPAQYFREIDEQRGYPERFVDALANAGWLAALIPQEYGGSGL 63 A D + D+RDAVRDLCK FP +Y+R+IDE RGYPE+FVDAL AGWLAALIPQEYGGSGL Sbjct: 6 ASDSHADLRDAVRDLCKTFPPEYWRKIDEARGYPEQFVDALTKAGWLAALIPQEYGGSGL 65 Query: 64 GLAEASVIMEEINRSGGNSGACHGQMYNMGTLLRHGSAEQKAKYLPDIASGKLRLQSMAV 123 GL EASVIMEEINR+GGNSGACHGQMYNMGTLLRHGS EQK +YLP IASG+LRLQSMAV Sbjct: 66 GLTEASVIMEEINRTGGNSGACHGQMYNMGTLLRHGSPEQKERYLPRIASGELRLQSMAV 125 Query: 124 TEPTTGTDTTKIKTTAVKKGDKYVVNGQKVWISRVQHSDLMILLARTTPLAEVKKKSEGM 183 TEPTTGTDTT+IKT AVKKGD+YV+NGQKVWISRVQHSDLMILLARTTPL +V +KSEGM Sbjct: 126 TEPTTGTDTTRIKTVAVKKGDRYVINGQKVWISRVQHSDLMILLARTTPLEQVSRKSEGM 185 Query: 184 SIFIVNLRDSIGKGLTVKPILNMVNHETNELFFDNLEIPAENLIGEEGQGFKYILTGLNA 243 SIF+V+L+++IG G+ V+PILNMVNHETNELFFDNLEIPAENLIGEEG+GF+YIL GLNA Sbjct: 186 SIFLVDLKEAIGHGMEVRPILNMVNHETNELFFDNLEIPAENLIGEEGRGFRYILDGLNA 245 Query: 244 ERTLIAAECIGDGYWFIEKARDYANERVVFGRPIGQNQGVQYPLARAFVNLEAANLMRFQ 303 ERTLIAAECIGDGYWFI+KA YA ERVVF RPIG NQGVQ+PLA A++ +EAANLMR+Q Sbjct: 246 ERTLIAAECIGDGYWFIDKATQYARERVVFDRPIGMNQGVQFPLAEAYIEVEAANLMRYQ 305 Query: 304 ACTRYDAHQDCGAQANMAKMLAADASWEAANACLQAHGGFGFACEYDVERKFRETRLYQV 363 AC +DA Q CGAQANMAK LAA A+WEAAN CLQ HGGFGFACEYDVERKFRETRLYQV Sbjct: 306 ACALFDAQQSCGAQANMAKYLAAKAAWEAANVCLQTHGGFGFACEYDVERKFRETRLYQV 365 Query: 364 APISTNMILSYVGEHVLKLPRSY 386 APISTN+I SYV EHVL LPRS+ Sbjct: 366 APISTNLIFSYVAEHVLGLPRSF 388 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 184 bits (466), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 122/374 (32%), Positives = 201/374 (53%), Gaps = 26/374 (6%) Query: 1 MLHAPDQYQDIRDAVRDLCKEFPAQYFREIDEQRGYPERFVDALANAGWLAALIPQEYGG 60 ML +P +++ IRDA+R +E + + D +P + LA G + +P+++GG Sbjct: 1 MLLSP-EHEMIRDAMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGG 59 Query: 61 SGLGLAEASVIMEEINRSGG---------NSGACHGQMYNMGTLLRHGSAEQKAKYLPDI 111 +G+ + +EEI G NS AC G ++GS QK ++L + Sbjct: 60 AGMDYMSLVLALEEIAAGDGATSTIVSVQNSLAC-------GITEKYGSPTQKEQWLKPL 112 Query: 112 ASGKLRLQSMAVTEPTTGTDTTKIKTTAVKKGDKYVVNGQKVWISRVQHSDLMILLARTT 171 A G++ L +TEP TG+D I+T A + GD +++NG K +I+ +H+ + I+ A T Sbjct: 113 ARGEM-LGCFCLTEPHTGSDAAAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVTD 171 Query: 172 PLAEVKKKSEGMSIFIVNLRDSIGKGLTVKPILNMVNH--ETNELFFDNLEIPAENLIGE 229 A K G+S F+V D+ G + + M H +T ++ DN +PA L+G+ Sbjct: 172 RSAGKK----GISCFLVPC-DTPGF-VVGRSEEKMGQHASDTVQIMLDNCRVPATALLGK 225 Query: 230 EGQGFKYILTGLNAERTLIAAECIGDGYWFIEKARDYANERVVFGRPIGQNQGVQYPLAR 289 EG+G++ L+ L A R IAA+ +G E A YA +R FG PI ++Q V + LA Sbjct: 226 EGEGYRIALSNLEAGRIGIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLAD 285 Query: 290 AFVNLEAANLMRFQACTRYDAHQDCGAQANMAKMLAADASWEAANACLQAHGGFGFACEY 349 L+AA LM ++A D + C +A+MAKM A++A+ + A+ +Q HGG G+ ++ Sbjct: 286 MNTLLDAARLMVWRAAQLKDQGRPCLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDF 345 Query: 350 DVERKFRETRLYQV 363 VER +R+ R+ Q+ Sbjct: 346 PVERIYRDVRICQI 359 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 180 bits (457), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 125/399 (31%), Positives = 211/399 (52%), Gaps = 28/399 (7%) Query: 1 MLHAP----DQYQDI---RDAVRDLCKEFPAQYFREIDEQRGYPERFVDALANAGWLAAL 53 M+H P D +DI R+AV A EID +P L + G L Sbjct: 1 MIHLPGLSFDHGEDIAALREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGIT 60 Query: 54 IPQEYGGSGLGLAEASVIMEEINRSGGNSGACHGQMYNM--GTLLRHGSAEQKAKYLPDI 111 + +EYGG+GLG + MEEI+R+ + G +G N+ + R+G+ EQK KYLP + Sbjct: 61 VSEEYGGAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRL 120 Query: 112 ASGKLRLQSMAVTEPTTGTDTTKIKTTAVKKGDKYVVNGQKVWISRVQHSDLMILLARTT 171 SG + ++A++EP G+D +K A KKGD+YV+NG K+WI+ +D++++ A+T Sbjct: 121 ISGDF-IGALAMSEPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKT- 178 Query: 172 PLAEVKKKSEGMSIFIVNLRDSIGKGLTVKPILN---MVNHETNELFFDNLEIPAENLIG 228 +++ + GM+ F+V + KG +V L+ M T EL F + E+P EN++G Sbjct: 179 ---DLEAGARGMTAFLV---EKGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLG 232 Query: 229 EEGQGFKYILTGLNAERTLIAAECIGDGYWFIEKARDYANERVVFGRPIGQNQGVQYPLA 288 G+G +++GL+ ER++++ +G ++ Y ++R FG+ IG+ Q +Q LA Sbjct: 233 GVGRGVNVLMSGLDFERSVLSGGPLGIMQACMDVVVPYVHDRKQFGQAIGEFQLMQGKLA 292 Query: 289 RAFVNLEAANLMRF---QACTRYDAHQDCGA---QANMAKMLAAD-ASWEAANACLQAHG 341 + + A + QAC R D+ A A A + +A+ A+W A A +Q+ G Sbjct: 293 DMYSTMMACKAYVYAVGQACDRADSADKVRALRKDAAGAILYSAEKATWMAGEA-IQSLG 351 Query: 342 GFGFACEYDVERKFRETRLYQVAPISTNMILSYVGEHVL 380 G G+ EY V R +R+ +LY++ ++ + +G + Sbjct: 352 GNGYINEYPVGRLWRDAKLYEIGAGTSEIRRMLIGRELF 390 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 167 bits (424), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 117/371 (31%), Positives = 191/371 (51%), Gaps = 17/371 (4%) Query: 11 IRDAVRDLCKEFPAQYFREIDEQRGYPERFVDALANAGWLAALIPQEYGGSGLGLAEASV 70 + + RD A + + DE+ +P + G+ P+ GG GL +A+V Sbjct: 12 FQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADGGLGLSRLDATV 71 Query: 71 IMEEINRSGGNSGACHGQMYNMGTLL--RHGSAEQKAKYLPDIASGKLRLQSMAVTEPTT 128 + EE+ R+ S A + ++NM + + G KA++ +A+G+ ++ S +TEP + Sbjct: 72 VFEELARAC-TSTAAYLTIHNMVSWMIASWGQPSLKAQWCEQLAAGR-KIGSYCLTEPGS 129 Query: 129 GTDTTKIKTTAVKKGDKYVVNGQKVWISRVQHSDLMILLARTTPLAEVKKKSEGMSIFIV 188 G+D +KTTA + YV+NG K +IS +D+++++ART + + G+S +V Sbjct: 130 GSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMARTG-----GEGARGVSAIVV 184 Query: 189 NLRD---SIGKGLTVKPILNMVNHETNELFFDNLEIPAENLIGEEGQGFKYILTGLNAER 245 S GK + + + T + FDN+++PA++L+G+EGQGF + + GL+ R Sbjct: 185 PGNAPGVSYGKK---ESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLDGGR 241 Query: 246 TLIAAECIGDGYWFIEKARDYANERVVFGRPIGQNQGVQYPLARAFVNLEAANLMRFQAC 305 IA +G ++ A Y ER FGRPI Q +Q+ LA L AA M A Sbjct: 242 INIATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKLADMQTELVAARQMVRLAA 301 Query: 306 TRYDA-HQDCGAQANMAKMLAADASWEAANACLQAHGGFGFACEYDVERKFRETRLYQVA 364 T+ DA + MAK LA D + N LQ HGG+G+ EY +ER FR+ R++Q+ Sbjct: 302 TKLDAGSPEATTYCAMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVHQIL 361 Query: 365 PISTNMILSYV 375 TN I+ + Sbjct: 362 E-GTNEIMRVI 371 >gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 415 Score = 114 bits (285), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 87/333 (26%), Positives = 158/333 (47%), Gaps = 40/333 (12%) Query: 7 QYQDIRDAVRDLCKEFPAQYFREI---------DEQRGYPER-FVDALANAGWLAALIPQ 56 Q + + A++ + F A R++ ++R Y R F A+A+AG++ AL+P+ Sbjct: 6 QLSESQKALQAGARAFAADVLRKVGPTITPLVKPDERFYATRPFYQAMADAGFIKALLPE 65 Query: 57 EYGGSGLGLAEASVIMEEINRSGGN-SGACHGQMYNMGTLLRHGSAEQKAKYLPDIASGK 115 +GG+ L + ++ EE+ N A G + ++ HG+ EQK ++LPD Sbjct: 66 SFGGTALSTLDFALAAEELTAVDVNVPSALLGTGLGLHAVVHHGTPEQKQRFLPDFIEDG 125 Query: 116 LRLQSMAVTEPTTGTD------TTKIKTTAVKKGDKYVVNGQKVWISRVQHSD-----LM 164 RL ++A TE T G + + ++T A +GD++V+NG K + + D L Sbjct: 126 SRLAALAFTEVTGGANYDSTDPSVGVQTFARLEGDEWVINGAKHYTTNGTGWDGKGAHLY 185 Query: 165 ILLARTTP-------LAEVKKKSEGMSIFIVNLRDSIGKGLTVKPILNMVNHETNELFFD 217 ++ RT P LA + I IV L D++G T+ P + F+ Sbjct: 186 SVICRTDPALPPQQSLAVIMVPGNAPGIEIVGLLDTMGHRATISP----------RVHFN 235 Query: 218 NLEIPAENLIGEEGQGFKYILTGLNAERTLIAAECIGDGYWFIEKARDYA-NERVVFGRP 276 N+ +PA N++G+ G G + + LI + C+G + A +A ++R P Sbjct: 236 NVRVPASNIVGKPGDGISMLEAAFSWTAALIGSACVGVMRTAFDIALRFAQSDRRSGTIP 295 Query: 277 IGQNQGVQYPLARAFVNLEAANLMRFQACTRYD 309 + ++Q V + LA + +EAA + +++C + D Sbjct: 296 VIEHQNVGFMLADIKMRIEAARYLTWKSCHQLD 328 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,140,569 Number of extensions: 45799 Number of successful extensions: 158 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 141 Number of HSP's successfully gapped: 9 Length of query: 386 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 299 Effective length of database: 1,207,925 Effective search space: 361169575 Effective search space used: 361169575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)