BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0138.p (327 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312411|ref|YP_003776503.1| NADH:flavin oxidoreductase/NADH... 317 8e-88 gi|300313850|ref|YP_003777942.1| xenobiotic reductase A [Herbasp... 105 7e-24 gi|300312734|ref|YP_003776826.1| NADH:flavin oxidoreductase [Her... 74 2e-14 >gi|300312411|ref|YP_003776503.1| NADH:flavin oxidoreductase/NADH oxidase family protein [Herbaspirillum seropedicae SmR1] Length = 366 Score = 317 bits (812), Expect = 8e-88, Method: Compositional matrix adjust. Identities = 180/361 (49%), Positives = 216/361 (59%), Gaps = 42/361 (11%) Query: 2 TSLFDPLKLGDISLANRFVMAPLTRSRAVDGFKPGPLTVEYYRQRATAGLIIAEATQISQ 61 + LF P++LG + L NR VMAPLTRSRA G P L EYY QRA AGLIIAEATQIS Sbjct: 8 SDLFKPVQLGAVQLQNRIVMAPLTRSRAQAGDVPSDLAAEYYAQRAGAGLIIAEATQISP 67 Query: 62 MGQGYLNTPGIYSPEXGVAPVSSTNRAANAK----------------------------- 92 G+GY TPGIY+ + VA A +AK Sbjct: 68 EGKGYAWTPGIYN-DAHVAAWKKITDAVHAKGGRIFLQLWHVGRISHPDLQPGHALPVAP 126 Query: 93 --------TFTTEGYSDVSPPRALRDDELPALVEDYRKAARNAIDAGFDGVEVHAANTYL 144 FT G+ PRAL ELPA+VE Y+KAA+ ++ AGFDGVE+HAAN YL Sbjct: 127 SAVQPEGQAFTESGFKPFVTPRALEAAELPAIVEQYKKAAQLSMQAGFDGVEIHAANGYL 186 Query: 145 LEQFLRDSVNDRSGPYGGSIENRARLVLEVLQAVAKEVGAGRTGIRLSPMTTFNDTAIDS 204 L+QFLRD N R+ YGGSIENRARL+LEV+QAV V + R GIRLSP++ ND A DS Sbjct: 187 LDQFLRDKTNQRTDGYGGSIENRARLLLEVVQAVTSVVPSERVGIRLSPISPANDIA-DS 245 Query: 205 DPQALYNYVVEQIASLGLAYLHVIEGETGGVRVPEGAPVKFDYAALRSRFPGAFVVNNGF 264 DP+AL++YVVEQ+ L YLHV+EG TGG P P FD LR F G ++ NNG+ Sbjct: 246 DPKALFSYVVEQLNRFKLVYLHVVEGATGG---PREVPGGFDLQVLRDLFKGLYIANNGY 302 Query: 265 DGAEGQAVVEAGKADAIAFGRPFIANPDLVRRVRESKPLNPLRAETLYGGGAEGYTDYPA 324 D + +AD +AFGRP+IANPDLV+R L TLYGGGA GYTDYP Sbjct: 303 DLDMALQARASNRADLVAFGRPWIANPDLVQRFLHGAELATFNQATLYGGGAAGYTDYPP 362 Query: 325 L 325 + Sbjct: 363 M 363 >gi|300313850|ref|YP_003777942.1| xenobiotic reductase A [Herbaspirillum seropedicae SmR1] Length = 362 Score = 105 bits (261), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 103/341 (30%), Positives = 150/341 (43%), Gaps = 55/341 (16%) Query: 1 MTSLFDPLKLGDISLANRFVMAPLTRSRAVDGFKPGPLTVEYYRQ--RATAGLIIAEATQ 58 M++LF P KL D+SL NR + P+ + AV+G K + +Y R AGL+I EAT Sbjct: 1 MSALFQPFKLKDVSLRNRIAVPPMCQYMAVEG-KVNDWHLSHYASIARGGAGLVIVEATA 59 Query: 59 ISQMGQGYLNTPGIYSPEXGVA--PVSSTNRAANA------------------------- 91 +S G+ GI+S G A PV +AA A Sbjct: 60 VSPEGRITPGCTGIWSDALGQAFVPVVQAIKAAGAVPGIQIGHAGRKASANRPWEGDDHI 119 Query: 92 KTFTTEGYSDVSP------------PRALRDDELPALVEDYRKAARNAIDAGFDGVEVHA 139 G+ +SP P+A+ D++ + +D+ AA A +AGF+ +E+H Sbjct: 120 AADDARGWDTLSPSAIAYGRGLPKVPKAMSLDDIARVRQDFVNAAVRAREAGFEWLELHF 179 Query: 140 ANTYLLEQFLRDSVNDRSGPYGGSIENRARLVLEVLQAVAKEVGAGRTGIRLSPMTTFND 199 A+ YL + F N R YGGS+ENRAR +LE L+AV +EV L+ F Sbjct: 180 AHGYLGQSFFSAHANQRDDLYGGSVENRARFLLETLRAV-REVWPE----HLALTVRFGV 234 Query: 200 TAIDS-DPQALYNY--VVEQIASLGLAYLHVIEGET-GGVRVPEGAPVKFDYAALRSRFP 255 D D + L VV Q G+ + V G T V++P G P A R R Sbjct: 235 LEYDGRDEETLMESIGVVRQFKDSGMDMISVTIGFTIPEVQIPWG-PAFLGPIAKRVREE 293 Query: 256 GAFVVNNGF---DGAEGQAVVEAGKADAIAFGRPFIANPDL 293 A V++ + A + V+ G+ D + GR + NP Sbjct: 294 AAVPVSSAWGFGTPALAEQAVKDGQLDLVMVGRSHLVNPHW 334 >gi|300312734|ref|YP_003776826.1| NADH:flavin oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 372 Score = 73.6 bits (179), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 64/216 (29%), Positives = 98/216 (45%), Gaps = 41/216 (18%) Query: 2 TSLFDPLKLGDISLANRFVMAPLTRSRAVDGFKPGPLTVEY-YRQRATAGLIIAEATQIS 60 + LF PL L ++L NR ++P+ + A DGF V R AGL++ EAT + Sbjct: 3 SHLFSPLPLRGVTLPNRIAVSPMCQYSAEDGFVNDWHLVHLGSRAVGGAGLVLMEATAVV 62 Query: 61 QMGQGYLNTPGIYSPEXGVAPVSSTNR----------------------------AANAK 92 G+ GI+ E +AP+ R A + Sbjct: 63 AEGRISSADLGIWKDEH-IAPLRRITRFIEQQGAVPGIQLAHAGRKSSILRPWDGPAGSV 121 Query: 93 TFTTEGYSDVSP-----------PRALRDDELPALVEDYRKAARNAIDAGFDGVEVHAAN 141 + G++ +P P+A+ +++ ALV+ + AA+ A+ AGF +E+H A+ Sbjct: 122 PPSAGGWTPQAPSALPFDDGYSTPQAMSVEQIGALVQAFADAAQRALAAGFKVIELHGAH 181 Query: 142 TYLLEQFLRDSVNDRSGPYGGSIENRARLVLEVLQA 177 YL QFL N RS YGGS ENR R +LE+ +A Sbjct: 182 GYLGHQFLSPLSNQRSDEYGGSFENRIRFLLEITRA 217 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.135 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,103,060 Number of extensions: 49060 Number of successful extensions: 153 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 151 Number of HSP's successfully gapped: 4 Length of query: 327 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 242 Effective length of database: 1,217,395 Effective search space: 294609590 Effective search space used: 294609590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)