BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0137.p (238 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 421 e-119 gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 148 4e-37 gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 121 6e-29 gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 59 3e-10 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 421 bits (1082), Expect = e-119, Method: Compositional matrix adjust. Identities = 195/238 (81%), Positives = 219/238 (92%) Query: 1 MAQNLEGRESHDREVVFRFEAPMRERAGFLVLKGNLFDFAIMKTSVISDSFRARYLSTPG 60 MA+NL+GR S+DRE+++ F AP+RERAGFLVLKGNLFDFAIMKTSVIS++FR RYLSTPG Sbjct: 357 MAENLQGRASNDREMIYPFAAPLRERAGFLVLKGNLFDFAIMKTSVISETFRERYLSTPG 416 Query: 61 SEGIFECTAAVFDGSDDYHARINDPSLGIDENTILVIRGAGPVGWPGSAEVVNMQPPDAL 120 E IFEC A VFDGSDDYHARINDP+L IDENT+L IRGAGPVGWPGSAEVVNMQPPDAL Sbjct: 417 QENIFECRAVVFDGSDDYHARINDPALKIDENTLLAIRGAGPVGWPGSAEVVNMQPPDAL 476 Query: 121 LRRGITTLPTLGDGRQSGTSDSPSILNASPESAVGGGLAYLHTGDKIRIDLNTGRCDMLI 180 ++RG++TLPTLGDGRQSGTSDSPSILNASPESAVGGGLAYL GD++RIDLNTG C+ML+ Sbjct: 477 IKRGVSTLPTLGDGRQSGTSDSPSILNASPESAVGGGLAYLRDGDRVRIDLNTGECNMLV 536 Query: 181 SDEELTRRKAEGIPAVPASQTPWQEIYRDTVGQLETGAVMELAVKYQGIAKVLPRHNH 238 S+EEL RRK+EGIP VP SQTPWQEIYR TVGQLETGA MELA+KYQG+A+ LPRHNH Sbjct: 537 SEEELARRKSEGIPPVPPSQTPWQEIYRSTVGQLETGACMELALKYQGVAQTLPRHNH 594 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 148 bits (373), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 90/241 (37%), Positives = 134/241 (55%), Gaps = 16/241 (6%) Query: 1 MAQNLEGRESHDREVVFRFEAPMRERAGFLVLKGNLF-DFAIMKTSVISDSFRARYLSTP 59 + +N + + + EV+ F+ P +E AG +LKGNL D A++K S + + L Sbjct: 351 LWENCQDAPNWNEEVIRSFDKPFKEAAGIAILKGNLAPDGAVIKPSAATPAL----LKHR 406 Query: 60 GSEGIFECTAAVFDGSDDYHARINDPSLGIDENTILVIRGAGPVGWPGSAEVVNMQPPDA 119 G A VF+ SDD H RI+D +L +DE +LV++ GP G+PG AE NM P Sbjct: 407 GR-------AVVFENSDDLHKRIDDENLDVDETCVLVLKNCGPKGYPGMAEAGNMPLPPK 459 Query: 120 LLRRGITTLPTLGDGRQSGTSDSPSILNASPESAVGGGLAYLHTGDKIRIDLNTGRCDML 179 +LR+GIT + + D R SGT+ +L+ SPE+A GG LA + GD I +D+ + + Sbjct: 460 ILRKGITDMVRVSDARMSGTAYGTVVLHVSPEAAAGGPLAVVQNGDFIELDVEARKLHLD 519 Query: 180 ISDEELTRRKAEG-IPAVPAS-QTPWQEIYRDTVGQLETGAVMELAVKYQGIAKVLPRHN 237 +SDEEL RR+A+ P +P Q W ++Y D V Q GA ++ V G +P+ N Sbjct: 520 VSDEELARRRAQWQKPELPPQMQRGWVKLYVDHVQQANQGADLDFLVGKSG--PYVPKDN 577 Query: 238 H 238 H Sbjct: 578 H 578 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 121 bits (303), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 83/247 (33%), Positives = 124/247 (50%), Gaps = 26/247 (10%) Query: 1 MAQNLEGRESHDREVVFRFEAPMRERAGFLVLKGNLFDF-AIMKTSVISDSF---RARYL 56 M N++ ++ EVV P+ E G +L+GNL A++K S + R R Sbjct: 356 MWDNVKDAPIYNDEVVRPLAKPLIEDGGICILRGNLAPRGAVLKPSAATPELMKHRGR-- 413 Query: 57 STPGSEGIFECTAAVFDGSDDYHARINDPSLGIDENTILVIRGAGPVGWPGSAEVVNMQP 116 A VF+ + Y RINDP L +D + +LV++ GP G+PG AEV NM Sbjct: 414 ------------AVVFEDFNHYKERINDPDLDVDASCVLVMKNVGPKGYPGMAEVGNMGL 461 Query: 117 PDALLRRGITTLPTLGDGRQSGTSDSPSILNASPESAVGGGLAYLHTGDKIRIDLNTGRC 176 P +L G+ + + D R SGT+ IL+ +PE+A GG L + GD I +D G+ Sbjct: 462 PPKVLATGVKDMVRISDARMSGTAYGTVILHVAPEAAAGGPLGIVQDGDFIELDAYAGKL 521 Query: 177 DMLISDEELTRRKAEGIPAVPASQTP-----WQEIYRDTVGQLETGAVMELAVKYQGIAK 231 + IS+EE+ RR E V A + P +Q +Y D V Q + G + V +G A Sbjct: 522 QLDISEEEMKRR-LEARAKVLAERKPEMVGGYQSLYVDRVLQADEGCDFDFLVGCRGAA- 579 Query: 232 VLPRHNH 238 +P+H+H Sbjct: 580 -VPKHSH 585 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 58.9 bits (141), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 50/172 (29%), Positives = 81/172 (47%), Gaps = 17/172 (9%) Query: 20 EAPMRERAGFLVLKGNLFDF-AIMKTSVISDSFRARYLSTPGSEGIFECTAAVFDGSDDY 78 E + G VL GN+ + I+KT+ + +S L G+ +FE A +G Sbjct: 417 EHAYSQDGGLAVLYGNIAEKGCIVKTAGVDESI----LKFSGTARVFESQDAAVEGILGD 472 Query: 79 HARINDPSLGIDENTILVIRGAGPVGWPGSAEVVNMQPPDALLRRGITTLPTL-GDGRQS 137 + D ++++R GP G PG E+ + P + +G+ L DGR S Sbjct: 473 KVKAGD---------VVIVRYEGPKGGPGMQEM--LYPTSYIKSKGLGKACALFTDGRFS 521 Query: 138 GTSDSPSILNASPESAVGGGLAYLHTGDKIRIDLNTGRCDMLISDEELTRRK 189 G S I +ASPE+A GG + + GD I ID+ ++ IS ++L +R+ Sbjct: 522 GGSSGLVIGHASPEAAEGGAIGLVEEGDMIDIDIPNRTINLRISSDDLAKRR 573 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.136 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 787,453 Number of extensions: 34952 Number of successful extensions: 101 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 99 Number of HSP's successfully gapped: 4 Length of query: 238 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 156 Effective length of database: 1,231,600 Effective search space: 192129600 Effective search space used: 192129600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)