BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0136.p (319 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 555 e-159 gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 242 3e-65 gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 234 8e-63 gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 103 2e-23 gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase ... 57 3e-09 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 555 bits (1431), Expect = e-159, Method: Compositional matrix adjust. Identities = 267/277 (96%), Positives = 274/277 (98%) Query: 5 HQTPRRFRSQDWFDNPDHIDMTALYLERFMNYGITAEELRSGRPIIGIAQSGSDISPCNR 64 + TPRRFRSQDWFDNPDHIDMTALYLERFMNYGITAEELRSGRPIIGIAQSGSDISPCNR Sbjct: 5 NATPRRFRSQDWFDNPDHIDMTALYLERFMNYGITAEELRSGRPIIGIAQSGSDISPCNR 64 Query: 65 IHLELARRVRDGIRDAGGIPMEFPLHPIFENCRRPTAAIDRNLSYLGLVEILHGYPIDAV 124 IHLELA+RVRDGIRDAGGIPMEFPLHPIFENCRRPTAAIDRNL+YLGLVEILHGYPIDAV Sbjct: 65 IHLELAKRVRDGIRDAGGIPMEFPLHPIFENCRRPTAAIDRNLAYLGLVEILHGYPIDAV 124 Query: 125 VLTTGCDKTTPAQIMAASTVDIPAIVLSGGPMLDGWMDGELVGSGAAIWKGRRQLSAGQI 184 VLTTGCDKTTP+QIMAA+TVDIPAIVLSGGPMLDGWMDGELVGSG+AIWKGR+ LSAG I Sbjct: 125 VLTTGCDKTTPSQIMAAATVDIPAIVLSGGPMLDGWMDGELVGSGSAIWKGRKLLSAGSI 184 Query: 185 DNEKFLEIAAASAPSSGHCNTMGTASTMNAMAEALGMSLTGCSAIPAPYRERGQMAYETG 244 DNEKFLEIAAASAPSSGHCNTMGTASTMNAMAEALGMSLTGCSAIPAPYRERGQMAYETG Sbjct: 185 DNEKFLEIAAASAPSSGHCNTMGTASTMNAMAEALGMSLTGCSAIPAPYRERGQMAYETG 244 Query: 245 RRIVGMAYEDLRPSAILTRDAFLDAIVVNAAIGGSTN 281 RRIVGMAYEDLRPSAILTRDAFLDAIVVNAAIGGSTN Sbjct: 245 RRIVGMAYEDLRPSAILTRDAFLDAIVVNAAIGGSTN 281 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 242 bits (617), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 137/294 (46%), Positives = 183/294 (62%), Gaps = 6/294 (2%) Query: 2 TNQHQTPRRFRSQDWFDNPDHIDMTALYLERFMNYGITAEELRSGRPIIGIAQSGSDISP 61 +++ R RS WF D +Y N GI E + G+P+IGI + S+++P Sbjct: 3 SDKKDKSRTLRSAGWFGTADKNGF--MYRSWMKNQGIPDHEFQ-GKPVIGICNTWSELTP 59 Query: 62 CNRIHLELARRVRDGIRDAGGIPMEFPLHPIFENCRRPTAAIDRNLSYLGLVEILHGYPI 121 CN ++A VR GI +AGG P+EFP+ E+ RPTA + RNL+ + + E + G PI Sbjct: 60 CNAHFRKIAEHVRRGIIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMDVEESIRGNPI 119 Query: 122 DAVVLTTGCDKTTPAQIMAASTVDIPAIVLSGGPMLDGWMDGELVGSGAAIWKGRRQLSA 181 DAVVL TGCDKTTPA +M A++ D+PAIV++GGPML+G G +GSG +W+ Q+ A Sbjct: 120 DAVVLLTGCDKTTPALLMGAASCDVPAIVVTGGPMLNGKHQGRDIGSGTVVWQLSEQVKA 179 Query: 182 GQIDNEKFLEIAAASAPSSGHCNTMGTASTMNAMAEALGMSLTGCSAIPAPYRERGQMAY 241 G+I F+ A + S+G CNTMGTASTM MAE+LG+SL +AIPA R +A+ Sbjct: 180 GEITIHDFMAAEAGMSRSAGTCNTMGTASTMACMAESLGVSLPHNAAIPAVDARRYVLAH 239 Query: 242 ETGRRIVGMAYEDLRPSAILTRDAFLDAIVVNAAIGGSTNXGRAGHHVGAATGR 295 +G RIV M +E L S ILTR AF +AI NAAIGGSTN A H+ A GR Sbjct: 240 LSGMRIVDMVWEGLTLSKILTRKAFENAIRTNAAIGGSTN---AVIHLKAIAGR 290 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 234 bits (596), Expect = 8e-63, Method: Compositional matrix adjust. Identities = 134/284 (47%), Positives = 173/284 (60%), Gaps = 6/284 (2%) Query: 12 RSQDWFDNPDHIDMTALYLERFMNYGITAEELRSGRPIIGIAQSGSDISPCNRIHLELAR 71 RSQ WF D +Y N GI ++ GRP+IGI + S+++PCN LA Sbjct: 11 RSQAWFGRQDRDGF--IYRSWVKNRGIPHDQF-DGRPVIGICNTYSELTPCNSHFRALAE 67 Query: 72 RVRDGIRDAGGIPMEFPLHPIFENCRRPTAAIDRNLSYLGLVEILHGYPIDAVVLTTGCD 131 +V+ GI +AGG P+EFP+ + E RPTA + RNL+ + + E + P+D VVL GCD Sbjct: 68 QVKIGIWEAGGFPLEFPVMSLGETLLRPTAMLYRNLASMDVEESIRANPLDGVVLLMGCD 127 Query: 132 KTTPAQIMAASTVDIPAIVLSGGPMLDGWMDGELVGSGAAIWKGRRQLSAGQIDNEKFLE 191 KTTPA +M A++VD+P I +SGGPML G G +GSG +W+ + AG++ E+F E Sbjct: 128 KTTPALLMGAASVDVPTIGVSGGPMLSGKYRGRELGSGTGVWQMSEDVRAGKMTQEEFFE 187 Query: 192 IAAASAPSSGHCNTMGTASTMNAMAEALGMSLTGCSAIPAPYRERGQMAYETGRRIVGMA 251 + S GHC TMGTASTM +M EALG+ L +AIPA R +A GRRIV M Sbjct: 188 AESCMHRSHGHCMTMGTASTMASMVEALGVGLPHNAAIPAVDARRNVLARNAGRRIVQMV 247 Query: 252 YEDLRPSAILTRDAFLDAIVVNAAIGGSTNXGRAGHHVGAATGR 295 EDL S ILTR AF +AI VNAAIGGSTN A H+ A GR Sbjct: 248 EEDLVLSKILTRQAFENAIRVNAAIGGSTN---AVIHLLAIAGR 288 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 103 bits (256), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 76/257 (29%), Positives = 131/257 (50%), Gaps = 28/257 (10%) Query: 47 RPIIGIAQSGSDISPCNRIHL-ELARRVRDGIRDAGGIPMEFPLHPIFE-----NCRRPT 100 +PII + S + P +HL +L + V I AGG+ EF + + + R Sbjct: 34 KPIIAVVNSFTQFVP-GHVHLKDLGQLVAREIEAAGGVAKEFNTIAVDDSGTHGDARMLY 92 Query: 101 AAIDRNLSYLGLVEILHGYPIDAVVLTTGCDKTTPAQIMAASTVDIPAIVLSGGPMLDGW 160 + R+L + +++ + DA+V + CDK TP +MAA ++IP + +SGGPM G Sbjct: 93 SLPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVVFVSGGPMEAGK 152 Query: 161 M------DGELVG---SGAAIWKGRRQLSAGQIDNEKFLEIAAASAPSSGHCNTMGTAST 211 + D +++ A I G + +S + E+ ++ P+ G C+ M TA++ Sbjct: 153 VIKTVNTDKKVIKLDLVDAMIQAGDKNISDADV-----AEVERSACPTCGSCSGMFTANS 207 Query: 212 MNAMAEALGMSLTGCSAIPAPYRERGQMAYETGRRIVGMA---YED----LRPSAILTRD 264 MN + E LG+SL G I A + +R ++ GR IV +A YE + P I T++ Sbjct: 208 MNCLTEVLGLSLPGNGTIVATHADRKELFLRAGRLIVELAKRHYEQDDYSILPRNICTKE 267 Query: 265 AFLDAIVVNAAIGGSTN 281 + +A+ ++ ++GGSTN Sbjct: 268 TWENAMTLDVSMGGSTN 284 >gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 623 Score = 56.6 bits (135), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 46/165 (27%), Positives = 76/165 (46%), Gaps = 11/165 (6%) Query: 121 IDAVVLTTGCDKTTPAQIMAASTV-DIPAIVLSGGPMLDGWMDGELVGSGAAIWKGRRQL 179 DA + CDK P ++ A +PA+ + GPM G + + V K R+ Sbjct: 147 FDAALYLGVCDKIVPGLLIGALHFGHLPAVFVPAGPMTSGMSNSDKV-------KIRQLY 199 Query: 180 SAGQIDNEKFLEIAAASAPSSGHCNTMGTASTMNAMAEALGMSLTGCSAIP--APYRE-R 236 +AG+I + LE + S +G C GTA++ + EA+G+ L G + I P R+ Sbjct: 200 TAGKIGRAELLEAESKSYHGAGTCTFYGTANSNQMLMEAMGLHLPGAAFITPNTPLRDAL 259 Query: 237 GQMAYETGRRIVGMAYEDLRPSAILTRDAFLDAIVVNAAIGGSTN 281 A + +I + + ++ + ++AIV A GGSTN Sbjct: 260 TAAAAKQAAKITDLGTQYTPVGRMIDEKSIVNAIVALHATGGSTN 304 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,027,235 Number of extensions: 45242 Number of successful extensions: 151 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 147 Number of HSP's successfully gapped: 5 Length of query: 319 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 234 Effective length of database: 1,217,395 Effective search space: 284870430 Effective search space used: 284870430 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)