BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0032.0135.p
         (293 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313173|ref|YP_003777265.1| gluconolactonase [Herbaspirillu...   191   9e-50
gi|300310377|ref|YP_003774469.1| gluconolactonase [Herbaspirillu...   151   6e-38

>gi|300313173|ref|YP_003777265.1| gluconolactonase [Herbaspirillum
           seropedicae SmR1]
          Length = 302

 Score =  191 bits (484), Expect = 9e-50,   Method: Compositional matrix adjust.
 Identities = 107/292 (36%), Positives = 150/292 (51%), Gaps = 8/292 (2%)

Query: 2   SPAAPTCVWPVGAELGEGPVWHAAAQAFCFVDIKGRAIHRCNAEGGQRESWSTPRQPGFV 61
           S AA T  +P    LGE P+WHA   A  ++DI G+A+HR + E GQ + W  P +PG +
Sbjct: 7   SAAAVTVAFPEPMLLGESPLWHAQEAALYWIDIPGKAVHRLHPESGQHKRWDLPEEPGSI 66

Query: 62  LPCDDGNLLCGMQGGLYRFDPKTGNFTLLQAVEEGLPGNRINDGFADHRGRLWFGTMDDA 121
           +    G L+  ++ G++  +  TG  TLL A        R NDG  D  GR W GT+ + 
Sbjct: 67  VRHAQGGLVVAIRTGMFHLNTDTGALTLLLAAPYDSSRIRFNDGRCDASGRFWCGTIYEP 126

Query: 122 EENASGALYCRDRGILREQDRNYIITNGPAMSPDGKTLYHTDTLLRTVYAFDVD-AAGNL 180
            +   G LYC +R  LR+     + +NG A S D + +YH +T    +  +D D A G  
Sbjct: 127 RDRDGGTLYCFERNALRDAHHAVVTSNGLAFSSDQRLMYHANTPAHRINLYDYDLATGAT 186

Query: 181 SGKRPFIVIDGS-------GYPDGMAVDAEGCLWVALFGGWRIERFLPSGELAGVQPFPC 233
           S  R     D         G PDG AVD++G  W A+F G R+ R  P GE+      P 
Sbjct: 187 SNCRLLRQFDSDKSAPDYGGRPDGAAVDSQGAYWCAMFEGGRVLRLSPQGEILQEIRLPA 246

Query: 234 ANITKLAFGGADLQTVQVTTAWKGLSPAERLQQPLAGGLFSFRADTPGLAQH 285
              T +AFGG DL+T+ +TT   G SPAE  Q P +G + + R D PG  ++
Sbjct: 247 RCPTMVAFGGDDLRTLYITTGRNGRSPAELAQYPESGCVLAVRVDVPGQLEY 298


>gi|300310377|ref|YP_003774469.1| gluconolactonase [Herbaspirillum
           seropedicae SmR1]
          Length = 292

 Score =  151 bits (382), Expect = 6e-38,   Method: Compositional matrix adjust.
 Identities = 97/276 (35%), Positives = 142/276 (51%), Gaps = 6/276 (2%)

Query: 15  ELGEGPVWHAAAQAFCFVDIKGRAIHRCNAEGGQRESWSTP-RQPGFVLPCDDGNLLCGM 73
           ELGEG +W   +Q+  + DI    +   + + G   SW  P R   +    D   LL G+
Sbjct: 12  ELGEGVLWCDRSQSVFWTDIHASRLWNHDPQTGLTRSWGMPERLCCYAFTADPQQLLIGL 71

Query: 74  QGGLYRFDPKTGNFTLLQAVEEGLPGNRINDGFADHRGRLWFGTMDDAEENASGALYCR- 132
           +  L  F+  TG    +  +E+ LP  R+NDG  D +GR  FGT+++    A  A + R 
Sbjct: 72  ESRLAFFNLSTGTIAPICRIEDDLPSTRLNDGRCDRQGRFVFGTLNEDAGRAPIASFYRL 131

Query: 133 --DRGILREQDRNYIITNGPAMSPDGKTLYHTDTLLRTVYAFDVD-AAGNLSGKRPFIVI 189
             D  + R    +  I+N    S DGK +YH D++   +   D D A+G + G+R F  +
Sbjct: 132 NTDLTLERLSLPSIAISNSICFSLDGKLMYHCDSMAGKIMVCDYDTASGAVGGQRVFADV 191

Query: 190 DGSGYPDGMAVDAEGCLWVALFGGWRIERFLPSGELAGVQPFPCANITKLAFGGADLQTV 249
              G PDG  +DAEG LW A +GG R+ R+ P G +  V   P +  + +AFGGA   T+
Sbjct: 192 AQPGGPDGSTIDAEGYLWNAQWGGARVVRYAPDGRIDRVVAIPTSQPSCVAFGGAQFDTL 251

Query: 250 QVTTAWKGLSPAERLQQPLAGGLFSFR-ADTPGLAQ 284
            VTTA +G+S  +R   P AG LF+   AD  GL +
Sbjct: 252 YVTTAHEGMSAEQRAADPQAGALFAVALADVRGLPE 287


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.321    0.140    0.454 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,138,355
Number of extensions: 54656
Number of successful extensions: 137
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 133
Number of HSP's successfully gapped: 2
Length of query: 293
Length of database: 1,619,870
Length adjustment: 84
Effective length of query: 209
Effective length of database: 1,222,130
Effective search space: 255425170
Effective search space used: 255425170
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 130 (54.7 bits)