BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0130.p (387 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313778|ref|YP_003777870.1| D-xylose ABC transporter permea... 550 e-157 gi|300310372|ref|YP_003774464.1| sugar ABC transporter permease ... 239 4e-64 gi|300310397|ref|YP_003774489.1| ribose/xylose/arabinose/galacto... 161 9e-41 gi|300310383|ref|YP_003774475.1| sugar ABC transporter permease ... 145 5e-36 gi|300310064|ref|YP_003774156.1| sugar ABC transporter permease ... 134 1e-32 >gi|300313778|ref|YP_003777870.1| D-xylose ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 385 Score = 550 bits (1416), Expect = e-157, Method: Compositional matrix adjust. Identities = 329/384 (85%), Positives = 356/384 (92%) Query: 4 TLSTGNTTANIKQLFSRYKIMALLIAIAIIWAFFSWKTEGGFVTPRNLSNLLRQMSVTGI 63 T ST + A++KQ+F RYKI+ALLIAIAIIW FFS+ T+GGFVTPRNLSNLLRQMSVTGI Sbjct: 2 TSSTLASMADLKQVFRRYKIIALLIAIAIIWGFFSFMTDGGFVTPRNLSNLLRQMSVTGI 61 Query: 64 LACGMVLVIIAGEIDLSVGSLLGLLGGVAAILNVTHNLPLPLNLFLVLLLGLLLGLFNGY 123 LACGMVLVIIAGEIDLSVGSLLGLLGG+AA+L+V+H+LPL LNL L L GL LGL NGY Sbjct: 62 LACGMVLVIIAGEIDLSVGSLLGLLGGLAALLDVSHHLPLALNLVLALGFGLALGLLNGY 121 Query: 124 LTAYMRIPSFIVGLGGMLAFRGVLLGITGGLTIAPVSSDLVYLGQGYLPASFGVALVVVL 183 LTAY+ IPSFIVGLGGMLAFRG+LLGITGGLTIAPVS ++VYLGQGYLP GVAL + L Sbjct: 122 LTAYLGIPSFIVGLGGMLAFRGILLGITGGLTIAPVSDNMVYLGQGYLPPQMGVALGIAL 181 Query: 184 FVLALALTWRQRVNRVRHALPVPPLWRDAIKVALIGAVLLAFVRTLNSYDGIPVPVLLLL 243 FVLA+ALTW RVNR RH LP+ LWRDA+KV +IGAVLLAFVRTLNSYDGIPVPVLLLL Sbjct: 182 FVLAVALTWMSRVNRSRHGLPLAALWRDALKVVVIGAVLLAFVRTLNSYDGIPVPVLLLL 241 Query: 244 ALLGLFSYITTQTVFGRRIYSVGSNMEATRLSGVNVQSVKLWVFGIMGVMCALAGLVNTA 303 ALLGLFSY++TQTVFGRRIYSVGSNMEATRLSGVNVQ+VKLWVFGIMGVMCALAGLVNTA Sbjct: 242 ALLGLFSYVSTQTVFGRRIYSVGSNMEATRLSGVNVQAVKLWVFGIMGVMCALAGLVNTA 301 Query: 304 RLAAGSPSSGSMGELDAIAACFIGGTSMRGGSGTIYGALIGALVMASLDNGMSMLDVGTY 363 RLAAGSPS+G+MGELDAIAACFIGGTSMRGGSGTIYGALIGALVMASLDNGMSMLDVGTY Sbjct: 302 RLAAGSPSAGNMGELDAIAACFIGGTSMRGGSGTIYGALIGALVMASLDNGMSMLDVGTY 361 Query: 364 WQMIVKGSILMLAVWVDVSTRSGR 387 WQMIVKGSIL+LAVWVDVSTRSGR Sbjct: 362 WQMIVKGSILVLAVWVDVSTRSGR 385 >gi|300310372|ref|YP_003774464.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 405 Score = 239 bits (609), Expect = 4e-64, Method: Compositional matrix adjust. Identities = 144/368 (39%), Positives = 225/368 (61%), Gaps = 8/368 (2%) Query: 26 LLIAIAIIWAFFSWKTEGGFVTPRNLSNLLRQMSVTGILACGMVLVIIAGEIDLSVGSLL 85 +L+++ I AFF T+G + P NL+NL+ Q S I+A GM++VI+AG IDLSVGS++ Sbjct: 37 MLMSLVAIMAFFQIMTDGTLMRPLNLTNLVLQNSYIVIMALGMLMVIVAGHIDLSVGSVV 96 Query: 86 GLLGGVAAILNVTHNLPLPLNLFLVLLLGLLLGLFNGYLTAYMRIPSFIVGLGGMLAFRG 145 GL+G +AA+L V + + L+ G L+G GY AY +IPSFIV L GML F+G Sbjct: 97 GLIGALAAVLMVDYGWGFVPASIVCLIAGGLIGAAQGYWIAYFKIPSFIVTLAGMLVFKG 156 Query: 146 VLLGITGGLTIAPVSSDLVYLGQGYLPA-----SFGVALVVVLFVLALAL---TWRQRVN 197 + L + G ++ P L G++P +F ++V + A+ L R Sbjct: 157 MALALLQGQSLGPFPQTFQMLSSGFIPELTGNTTFRTTSLIVGVIAAVVLILVKLHGRRK 216 Query: 198 RVRHALPVPPLWRDAIKVALIGAVLLAFVRTLNSYDGIPVPVLLLLALLGLFSYITTQTV 257 + +H + P+ +K + A ++AF L++Y G+P ++++ AL+ L+++IT++T Sbjct: 217 QTKHGMEDEPVLFFLLKNGVFAAAIIAFSYLLSTYRGMPNVLIIMFALMVLYTFITSRTT 276 Query: 258 FGRRIYSVGSNMEATRLSGVNVQSVKLWVFGIMGVMCALAGLVNTARLAAGSPSSGSMGE 317 GRR+Y+VG N +A +LSG+ + V + F MGV+ ALAGL+ ARL +P +G E Sbjct: 277 LGRRVYAVGGNEKAAKLSGIKTERVSFFTFVNMGVLAALAGLIFAARLNTATPKAGLGFE 336 Query: 318 LDAIAACFIGGTSMRGGSGTIYGALIGALVMASLDNGMSMLDVGTYWQMIVKGSILMLAV 377 LD IAACFIGG S GG G + GA+IGA VM ++NGMS++ +G +Q ++KG +L++AV Sbjct: 337 LDVIAACFIGGASASGGVGKVMGAVIGAFVMGVMNNGMSIMGIGIDYQQMIKGFVLLMAV 396 Query: 378 WVDVSTRS 385 DV ++ Sbjct: 397 CFDVYNKN 404 >gi|300310397|ref|YP_003774489.1| ribose/xylose/arabinose/galactoside ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 328 Score = 161 bits (407), Expect = 9e-41, Method: Compositional matrix adjust. Identities = 122/340 (35%), Positives = 173/340 (50%), Gaps = 61/340 (17%) Query: 45 FVTPRNLSNLLRQMSVTGILACGMVLVIIAGEIDLSVGSLLGLLGGVAAILNVTHNLPLP 104 F T N N+LRQ+S+ GILA GM VI+ G IDLSVG+ + L G ++A L V LP P Sbjct: 50 FFTLSNWFNVLRQVSIVGILAVGMSFVILTGGIDLSVGAAMALAGTISAGLIVNSGLPAP 109 Query: 105 LNLFLVLLLGLLLGLFNGYLTAYMRIPSFIVGLGGMLAFRGVLLGITGGLTIAPVSSDLV 164 L L + L +GL NG L A+ R+P+ IV LA GV G+ L+ Sbjct: 110 LALLCGVGLATCIGLLNGALVAWGRMPAIIV----TLATMGVARGV-----------GLI 154 Query: 165 YLGQGYLPASFGVALVVVLFVLALALTWRQRVNRVRHALPVPPLWRDAIKVALIGAVLLA 224 Y G GY + P W V IG V Sbjct: 155 YSG-GYPISGL-------------------------------PGWISWFGVGRIGMV--- 179 Query: 225 FVRTLNSYDGIPVPVLLLLALLGLFSYITTQTVFGRRIYSVGSNMEATRLSGVNVQSVKL 284 PVPV+L+L + L + +T FGR +Y++G N A RLSGV +KL Sbjct: 180 -----------PVPVILMLIVYALAWLLLQRTAFGRHVYAIGGNEMAARLSGVKTTRIKL 228 Query: 285 WVFGIMGVMCALAGLVNTARLAAGSPSSGSMGELDAIAACFIGGTSMRGGSGTIYGALIG 344 V+ I G LA ++ T RL +G P++G ELDAIAA +GGT++ GG G + G LIG Sbjct: 229 AVYAISGFTSGLAAIILTGRLMSGQPNAGVGFELDAIAAVVLGGTAIAGGRGLVVGTLIG 288 Query: 345 ALVMASLDNGMSMLDVGTYWQMIVKGSILMLAVWVDVSTR 384 A+++ L+NG++++ + Y Q I++G I++LA+++ R Sbjct: 289 AVLLGILNNGLNLMGINPYLQDIIRGVIILLAIYIAREWR 328 >gi|300310383|ref|YP_003774475.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 347 Score = 145 bits (367), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 124/387 (32%), Positives = 196/387 (50%), Gaps = 55/387 (14%) Query: 1 MTSTLSTGNTTANIKQLFSRYKIMALLIAIAIIWAFFSWKTEGGFVTPRNLSNLLRQMSV 60 M +T S A + +R K++A ++ ++ FFS+ + F+ NL ++L+ +V Sbjct: 15 MANTASAQGLRARLFNPAARQKLLAFA-SLLLMILFFSFASPN-FMEVDNLVSILQSTAV 72 Query: 61 TGILACGMVLVIIAGEIDLSVGSLLGLLGGVAAILNVTHNLPLPLNLFLVLLLGLLLGLF 120 G+LA VII IDLSVG+++ +A ++ +PLPL + + G L G Sbjct: 73 NGVLAIACTYVIITSGIDLSVGTMMTFCAVMAGVVLTNWGMPLPLGIAAAIFFGALSGWI 132 Query: 121 NGYLTAYMRIPSFIVGLGGMLAFRGVLLGITGGLTIAPVSSDLVYLGQGYLPASFGVALV 180 +G + A +++P FI LG M+ +G+ L I+G I Y + G + Sbjct: 133 SGMVIAKLKVPPFIATLGMMMLLKGLSLVISGTRPI-------------YFNDTEGFS-- 177 Query: 181 VVLFVLALALTWRQRVNRVRHALPVPPLWRDAIKVALIGAVLLAFVRTLNSYDGIPVPVL 240 A+ + + +LP+P AVL+ F+ + + Sbjct: 178 --------AIAQDSLIGDLIPSLPIP------------NAVLILFLVAIGA--------- 208 Query: 241 LLLALLGLFSYITTQTVFGRRIYSVGSNMEATRLSGVNVQSVKLWVFGIMGVMCALAGLV 300 S I +TVFGR +++GSN EA RLSGV V K+ V+ G +C +AGL+ Sbjct: 209 ---------SIILNKTVFGRYTFALGSNEEALRLSGVKVDFWKVAVYTFSGAICGIAGLI 259 Query: 301 NTARLAAGSPSSGSMGELDAIAACFIGGTSMRGGSGTIYGALIGALVMASLDNGMSMLDV 360 +RL + P+ G ELDAIAA IGGTS+ GG+GTI G +IGA +M+ L NG+ ++ V Sbjct: 260 IASRLNSAQPALGQGYELDAIAAVVIGGTSLSGGTGTILGTIIGAFIMSVLVNGLRIMSV 319 Query: 361 GTYWQMIVKGSILMLAVWVDVSTRSGR 387 WQ +V G I++LAV++D+ R R Sbjct: 320 AQEWQTVVTGVIIILAVYLDILRRRRR 346 >gi|300310064|ref|YP_003774156.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 344 Score = 134 bits (336), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 108/353 (30%), Positives = 180/353 (50%), Gaps = 78/353 (22%) Query: 36 FFSWKTEGGFVTPRNLSNLLRQMSVTGILACGMVLVIIAGEIDLSVGSLLGLLG--GVAA 93 + S F + N N+LRQ+++ +LA GM VI+ IDLSVGS+L + G+ Sbjct: 51 YLSGDGTSNFASAENTMNILRQVAINLVLAAGMTFVILTAGIDLSVGSVLAVSAVLGMQV 110 Query: 94 ILNVTHNLPLPLNLFLVLLLGLLLGLFNGYLTAYMRIPSFIVGLGGMLAFRGVLLGITGG 153 L +P+ +F GL++G+ NG + A + I +F+V LG M AFRG + G Sbjct: 111 SLGAAPGWAIPMFIF----SGLVMGMVNGAMVALLNINAFVVTLGTMTAFRGAAYLLADG 166 Query: 154 LTIAPVSSDL---VYLGQG---YLPASFGVALVVVLFVLALALTWRQRVNRVRHALPVPP 207 T+ +++D+ ++G G ++P VA+ VVL L+W Sbjct: 167 TTV--LNNDIPSFEWIGNGDFLHVPWLIWVAVAVVL------LSW--------------- 203 Query: 208 LWRDAIKVALIGAVLLAFVRTLNSYDGIPVPVLLLLALLGLFSYITTQTVFGRRIYSVGS 267 V+L +LG+ Y ++G Sbjct: 204 -------------------------------VILRKTVLGMHIY------------AIGG 220 Query: 268 NMEATRLSGVNVQSVKLWVFGIMGVMCALAGLVNTARLAAGSPSSGSMGELDAIAACFIG 327 N++A RL+G+ V V L+V+ I G+ LAG ++ +RL + + GS ELDAIAA +G Sbjct: 221 NLQAARLTGIRVGLVLLFVYSISGLFSGLAGAMSASRLYGANGNWGSGYELDAIAAVVLG 280 Query: 328 GTSMRGGSGTIYGALIGALVMASLDNGMSMLDVGTYWQMIVKGSILMLAVWVD 380 GTS+ GG G+I+G ++GAL++ ++NG+++L + ++WQ + KG++++LAV +D Sbjct: 281 GTSLMGGVGSIWGTVVGALIIGVMNNGLTILGLSSFWQYVAKGAVIVLAVILD 333 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.142 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 942,003 Number of extensions: 33648 Number of successful extensions: 276 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 272 Number of HSP's successfully gapped: 24 Length of query: 387 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 300 Effective length of database: 1,207,925 Effective search space: 362377500 Effective search space used: 362377500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)