BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0111.p (831 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312928|ref|YP_003777020.1| transmembrane acyl-CoA dehydrog... 1278 0.0 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 117 3e-27 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 99 9e-22 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 87 6e-18 >gi|300312928|ref|YP_003777020.1| transmembrane acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 824 Score = 1278 bits (3306), Expect = 0.0, Method: Compositional matrix adjust. Identities = 631/821 (76%), Positives = 705/821 (85%), Gaps = 3/821 (0%) Query: 1 MILWLVLFVAGALALMMMRAGAWTWLVAELAWIWIGGTTDQANAVVCILLAILLCVPTLL 60 MI+WL+L +A ALALMMMRA AW WL AE +W+G + V +LLAIL PTLL Sbjct: 1 MIIWLILLIASALALMMMRASAWAWLAAEAVLLWLGARLAEIEWPVLVLLAILFFTPTLL 60 Query: 61 VAITSVRQTLITRPALDIFRKILPGMSATERDAIEAGTVWWDAELFSGKPDWNKLMQLPP 120 VAI SVRQ L+TRPALDIFRKI+P MSATERDAIEAGTVWWDAELFSGKPDW++L LP Sbjct: 61 VAIRSVRQALMTRPALDIFRKIMPAMSATERDAIEAGTVWWDAELFSGKPDWSRLFALPA 120 Query: 121 PTLTAEEQSFLDNEVTQLCAMVSDWDTSVVWQDLQPEAWQFVKDKGFLGMIIPKQYGGKQ 180 P LTAEEQ+FLDNEV QLC MVSDWD +V QDL PEAWQF+KDKGFLGMIIP+QYGGKQ Sbjct: 121 PRLTAEEQAFLDNEVEQLCGMVSDWDATVKTQDLPPEAWQFIKDKGFLGMIIPRQYGGKQ 180 Query: 181 FSAYMHSQVIMKLSSRCSVAAISVMVPNSLGPAELLLHYGTEEQKNHYLPRLARGEEIPC 240 FSAYMHSQV+MKL+SR S AAI+VMVPNSLGPAELLLHYGTE QKNHYLPRLA+G EIPC Sbjct: 181 FSAYMHSQVVMKLASRSSAAAIAVMVPNSLGPAELLLHYGTEVQKNHYLPRLAQGLEIPC 240 Query: 241 FALTSPYAGSDAAAIPDVGVVCKGVHEGREVIGFRVTWAKRYITLAPVATVLGLAFRAHD 300 FALTSPYAGSDAAAIPD+G+VCKGVHEGRE +GFRVTW+KRYITLAP+ATVLGLAFRA D Sbjct: 241 FALTSPYAGSDAAAIPDIGIVCKGVHEGRETLGFRVTWSKRYITLAPIATVLGLAFRASD 300 Query: 301 PDHLLSDNSEPGITCALIPTSHPGVVTGRRHWPLNAVFQNGPTSGENVFIPLDWVIGGQP 360 PD LL E GITCALIPT HPGVVTGRRHWPLNAVFQNGPT G++VFIP++WVIGG Sbjct: 301 PDGLLGGEREAGITCALIPTDHPGVVTGRRHWPLNAVFQNGPTEGKDVFIPMEWVIGGVA 360 Query: 361 QVGKGWRMLMECLAAGRAISLPSSSVGFSKMAVRGTSAYTAMRRQFKIAIGKFEGIQEAL 420 QVG+GWRMLMECLAAGRAISLPSSSVGFSKMAVRGTS Y AMRRQFKI IGKFEG+QEAL Sbjct: 361 QVGRGWRMLMECLAAGRAISLPSSSVGFSKMAVRGTSGYAAMRRQFKIEIGKFEGVQEAL 420 Query: 421 ARMGGNLYMMDATRRLSSLAVDAGEKPSVISAISKYHVTERGRMLVNDGMDVIGGKGICM 480 ARMGG+LYMMDATRRLS+LAVDAGEKPSVISAISKYH+TERGRM+VNDGMDVIGGKGICM Sbjct: 421 ARMGGHLYMMDATRRLSALAVDAGEKPSVISAISKYHITERGRMVVNDGMDVIGGKGICM 480 Query: 481 GPGNFLARVYQQIPIAITVEGANILTRCLIIFGQGAIRCHPYVLKEMAAAADDDHDRALQ 540 GPGNFLARVYQQIPIAITVEGANILTRCLIIFGQGAIRCHPYVLKE+AAAAD+D +RA+Q Sbjct: 481 GPGNFLARVYQQIPIAITVEGANILTRCLIIFGQGAIRCHPYVLKELAAAADEDRERAVQ 540 Query: 541 DFDRAFFGHLSFVAANMARSLVAGLSGGLLASAPGPDQAAEETRAYYRAVARVSACFSLL 600 +FDR FGHLSFVAAN ARSLV GLSGG L +A P QAA TR YYRAV +S+ F+LL Sbjct: 541 EFDRLLFGHLSFVAANAARSLVGGLSGGWLLAA--PAQAAHATRRYYRAVVHLSSAFALL 598 Query: 601 TDVSMFVLGGSLKFRERISGRLGDVLSQLYLISATLKRYEDDGRQSADLPYVAWSVQDAL 660 TD SM VLGG+LKFRERIS RLGDVLSQLYL+SA LKRYED+GRQ ADLPYV WSVQ+AL Sbjct: 599 TDASMAVLGGTLKFRERISARLGDVLSQLYLVSAALKRYEDEGRQEADLPYVDWSVQEAL 658 Query: 661 HRAQEAIIDVLENFPNPFIAFALRIWMFPLGLSFRKPSDRLASDVALAMQTPGAARDRLV 720 +RAQEAI++VL NFPNPF+AFALR+ +FPLGL +RKP+D L + VA AMQTPG +R+RL+ Sbjct: 659 NRAQEAIVEVLHNFPNPFVAFALRLAIFPLGLPYRKPADVLGTHVAKAMQTPGESRERLL 718 Query: 721 ADAYRPDFRTDPLGSGEHAFDLLPQVHEIETRLKQSMKDGVLPAMPQSLIEMRRWNARAM 780 ADAY P DPL GE AF L P V ++E RLK +++G LP MPQSL+E+ W+A A+ Sbjct: 719 ADAYLPR-AGDPLACGEQAFALTPVVQQLEHRLKPDIREGKLPPMPQSLLELTAWSALAL 777 Query: 781 EQGLISIDERVVLDDFAHYGDLTVQVDDFDAGFDMQEAVQK 821 E+GL+S+ E+ LD FA YG +V VDDF A FD E V + Sbjct: 778 ERGLLSLQEKEALDAFALYGHQSVAVDDFAADFDRAEMVAQ 818 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 117 bits (294), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 105/383 (27%), Positives = 174/383 (45%), Gaps = 41/383 (10%) Query: 137 QLCAMVSDWDTSVVWQDLQPEAWQFVKDKGFLGMIIPKQYGGKQFSAYMHSQVIMKLSSR 196 +L +DWD + EA + + + G +GM +P+Q+GG + ++++ Sbjct: 21 RLLPFAADWDRN---HTFPAEALKELAELGAMGMCVPEQWGGAGMDYMSLVLALEEIAAG 77 Query: 197 CSVAAISVMVPNSLGPAELLLHYGTEEQKNHYLPRLARGEEIPCFALTSPYAGSDAAAIP 256 + V V NSL + YG+ QK +L LARGE + CF LT P+ GSDAAAI Sbjct: 78 DGATSTIVSVQNSLACG-ITEKYGSPTQKEQWLKPLARGEMLGCFCLTEPHTGSDAAAIR 136 Query: 257 DVGVVCKGVHEGREVIGFRVTWAKRYITLAPVATVLGLAFRAHDPDHLLSDNSEPGITCA 316 R+ F + K++IT A V + F D + GI+C Sbjct: 137 --------TRAERDGDDFILNGTKQFITSGKHAGV-AIVFAVTD-----RSAGKKGISCF 182 Query: 317 LIPTSHPGVVTGRRHWPLNAVFQNGPTSGE----NVFIPLDWVIGGQPQVGKGWRMLMEC 372 L+P PG V GR + Q+ + + N +P ++G + G+G+R+ + Sbjct: 183 LVPCDTPGFVVGRSEEKMG---QHASDTVQIMLDNCRVPATALLGKE---GEGYRIALSN 236 Query: 373 LAAGRAISLPSSSVGFSKMAVRGTSAYTAMRRQFKIAIGKFEGIQEALARMGGNLYMMDA 432 L AGR I + + SVG ++ A +Y R F + I + + + LA M ++DA Sbjct: 237 LEAGR-IGIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADMN---TLLDA 292 Query: 433 TR----RLSSLAVDAGEKPSVISAISKYHVTERGRMLVNDGMDVIGGKGICMGPGNF-LA 487 R R + L D G ++++K +E + +D + + GG G +F + Sbjct: 293 ARLMVWRAAQLK-DQGRPCLKEASMAKMFASEAAEKIASDAIQIHGGVGY---TSDFPVE 348 Query: 488 RVYQQIPIAITVEGANILTRCLI 510 R+Y+ + I EGAN + R +I Sbjct: 349 RIYRDVRICQIYEGANDIQRLVI 371 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 99.4 bits (246), Expect = 9e-22, Method: Compositional matrix adjust. Identities = 102/392 (26%), Positives = 182/392 (46%), Gaps = 36/392 (9%) Query: 125 AEEQSFLDNEVTQLCAMVSDWDTSVVWQ-DLQPEAWQFVKDKGFLGMIIPKQYGGKQFSA 183 A +QS D ++ + WD + D+ +A + GF G+ P+ GG S Sbjct: 11 AFQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGEL----GFCGLYTPEADGGLGLSR 66 Query: 184 YMHSQVIMKLSSRCSVAAISVMVPNSLGPAELLLHYGTEEQKNHYLPRLARGEEIPCFAL 243 + V +L+ C+ A + + N + + ++ +G K + +LA G +I + L Sbjct: 67 LDATVVFEELARACTSTAAYLTIHNMV--SWMIASWGQPSLKAQWCEQLAAGRKIGSYCL 124 Query: 244 TSPYAGSDAAAIPDVGVVCKGVHEGREVIGFRVTWAKRYITLAPVATVLGLAFRAHDPDH 303 T P +GSDAA++ + G H + + +K +I+ A VL + R Sbjct: 125 TEPGSGSDAASLKTTARLEDG-H-------YVLNGSKAFISGAGSTDVLVVMART----- 171 Query: 304 LLSDNSEPGITCALIPTSHPGVVTGRRHWPLNAVFQNGPT---SGENVFIPLDWVIGGQP 360 G++ ++P + PGV G++ + + + PT S +NV +P D ++G Sbjct: 172 --GGEGARGVSAIVVPGNAPGVSYGKKESKMG--WNSQPTRTISFDNVKVPADHLLG--- 224 Query: 361 QVGKGWRMLMECLAAGRAISLPSSSVGFSKMAVRGTSAYTAMRRQFKIAIGKFEGIQEAL 420 Q G+G+ M+ L GR I++ + SVG ++ A+ AY R+QF I F+ +Q L Sbjct: 225 QEGQGFIFAMKGLDGGR-INIATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKL 283 Query: 421 ARMGGNLYMMDATRRLSSLAVDAGE-KPSVISAISKYHVTERGRMLVNDGMDVIGGKG-I 478 A M L RL++ +DAG + + A++K T+ G + N+ + + GG G I Sbjct: 284 ADMQTELVAARQMVRLAATKLDAGSPEATTYCAMAKRLATDLGFRVCNEALQLHGGYGYI 343 Query: 479 CMGPGNFLARVYQQIPIAITVEGANILTRCLI 510 P L R ++ + + +EG N + R +I Sbjct: 344 REYP---LERYFRDVRVHQILEGTNEIMRVII 372 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 86.7 bits (213), Expect = 6e-18, Method: Compositional matrix adjust. Identities = 84/329 (25%), Positives = 145/329 (44%), Gaps = 32/329 (9%) Query: 159 WQFVKDKGFLGMIIPKQYGGKQFSAYMHSQVIMKLSSRCSVAAISVMVPNSLGPAELLLH 218 W+ + D G LG+ + ++YGG H + ++S + +S ++L ++ + Sbjct: 48 WKKLGDLGVLGITVSEEYGGAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRN 107 Query: 219 YGTEEQKNHYLPRLARGEEIPCFALTSPYAGSDAAAIPDVGVVCKGVHEGREVIGFRVTW 278 G EEQK YLPRL G+ I A++ P AGSD VV + ++ + + Sbjct: 108 -GNEEQKRKYLPRLISGDFIGALAMSEPNAGSD--------VVSMKLRADKKGDRYVLNG 158 Query: 279 AKRYITLAPVATVLGLAFRAHDPDHLLSDNSEPGITCALIPTSHPGVVTGRRHWPLNAVF 338 +K +IT P A VL + + D + G+T L+ + G ++ L+ + Sbjct: 159 SKMWITNGPDADVL-VVYAKTDL-----EAGARGMTAFLVEKGYKGFSVAQK---LDKLG 209 Query: 339 QNGPTSGENVF----IPLDWVIGGQPQVGKGWRMLMECLAAGRAISLPSSSVGFSKMAVR 394 G +GE VF +P + V+GG VG+G +LM L R++ L +G + + Sbjct: 210 MRGSHTGELVFQDCEVPEENVLGG---VGRGVNVLMSGLDFERSV-LSGGPLGIMQACMD 265 Query: 395 GTSAYTAMRRQFKIAIGKFEGIQEALARMGGNLYMMDATRRLSSLAVDAGEKPSVISAIS 454 Y R+QF AIG+F+ +Q LA M + A A D + + A+ Sbjct: 266 VVVPYVHDRKQFGQAIGEFQLMQGKLADMYSTMMACKAYVYAVGQACDRADSADKVRALR 325 Query: 455 K------YHVTERGRMLVNDGMDVIGGKG 477 K + E+ + + + +GG G Sbjct: 326 KDAAGAILYSAEKATWMAGEAIQSLGGNG 354 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,525,227 Number of extensions: 107091 Number of successful extensions: 292 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 287 Number of HSP's successfully gapped: 5 Length of query: 831 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 738 Effective length of database: 1,179,515 Effective search space: 870482070 Effective search space used: 870482070 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 135 (56.6 bits)