BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0109.p (239 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 163 1e-41 gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 75 4e-15 gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 75 7e-15 gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspiri... 69 3e-13 gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Her... 60 1e-10 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 163 bits (412), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 94/209 (44%), Positives = 122/209 (58%), Gaps = 6/209 (2%) Query: 33 MQTRKLARDGLTVPEVGLGCWQLGGGWGDHWDNDVAQQTLAQAYATGVRFIDTADVYGDG 92 M++R+ R G V E+G G W +GG WGD D A+ L A GV FIDTADVYGDG Sbjct: 1 MKSRRFGRTGAQVSEIGFGAWAIGGSWGDVSAQD-AKAALHAALDAGVSFIDTADVYGDG 59 Query: 93 ASERSIGQFLTSH--KDLTVATKLGR--GAIYPDGYSREAVRTA-TQRSLERLGVDKLDL 147 SE+ I Q L + VATK GR GY+ A +A RSL+ LGVD LDL Sbjct: 60 RSEQLIAQVLKERGGERPFVATKAGRRLSPHVASGYNDRAQLSAFIDRSLKNLGVDCLDL 119 Query: 148 TQLHCVPPAVLKDGKIFDWMRELRDDGLITRFGASVETVEEGLLCLEQEGMTSLQVIFNI 207 QLHC P V ++F + L G I +G SVE VEE L +E + S+Q+IFNI Sbjct: 120 VQLHCPPTEVYYQQEVFGHLDALVAAGKIRHYGVSVEKVEEALKAIEYPNVKSVQIIFNI 179 Query: 208 FRQKPLADLLPQAKAKGVGIIVRLPLASG 236 FRQ+P++ L +A+ + + +I R+PLASG Sbjct: 180 FRQRPISLFLQEAQRRDIAVIARVPLASG 208 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 75.1 bits (183), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 65/229 (28%), Positives = 111/229 (48%), Gaps = 27/229 (11%) Query: 27 QPQGQHMQ------TRKLARDGLTVPEVGLGCWQLGGGWGDHWDNDVAQQTLAQAYATGV 80 +P H+Q TR+L +G V +GLGC + + D+ + T+ +A G+ Sbjct: 3 EPLTSHLQAHSPVGTRRLGSNGPLVSAIGLGCMGMSDFYAQR-DDAESLATIDRALELGI 61 Query: 81 RFIDTADVYGDGASERSIGQFLTSHKD-LTVATKLG-------RGAIYPDGYSREAVRTA 132 +DTAD+YG +E +G+ + +D +ATK G GA DG S +R A Sbjct: 62 NLLDTADMYGPYTNEELLGRAIKGRRDKFFIATKFGIRRDPSDPGARGVDG-SPAYIRQA 120 Query: 133 TQRSLERLGVDKLDLTQLHCVPPAV---LKDGKIFDWMR--ELRDDGLITRFGASVETVE 187 + SL+RLGV+ +DL H + PA + G + D ++ ++R GL A++E Sbjct: 121 VEGSLKRLGVETIDLYYQHRIDPATPIEVTMGVLADLVQAGKIRYIGLSEASAATLERAH 180 Query: 188 EGLLCLEQEGMTSLQVIFNIFRQKPLADLLPQAKAKGVGIIVRLPLASG 236 +T+LQ ++++ + P A++L + G+G + PL G Sbjct: 181 ------RVHPVTALQSEYSLWTRDPEAEVLAACRTLGIGFVAYSPLGRG 223 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 74.7 bits (182), Expect = 7e-15, Method: Compositional matrix adjust. Identities = 65/217 (29%), Positives = 98/217 (45%), Gaps = 21/217 (9%) Query: 33 MQTRKLARDGLTVPEVGLGCWQLGGGWGDHWDNDVAQQTLAQAYATGVRFIDTADVYGDG 92 M R+L + L V + LG +GD D + + +A A GV FIDTADVY G Sbjct: 1 MHYRRLGKSNLKVSALCLGSMM----FGDQTDLQESGRIVASAREHGVNFIDTADVYTKG 56 Query: 93 ASERSIGQFLTSHK-DLTVATKLGR---GAIYPDGYSREAVRTATQRSLERLGVDKLDLT 148 SE +G+ L + + +A+KLG G YSR + T+ L RL D LD+ Sbjct: 57 KSEEIVGKLLAGQRHEWVLASKLGNAMSGKPNESNYSRSWIVRETENILTRLDTDYLDIL 116 Query: 149 QLHCVPPAVLKDGKIFDWMRELRD---DGLITRFGASV----ETVEEGLLCLEQEGMTSL 201 +H +D + + ++ L D G I FG S E LC + L Sbjct: 117 YMH----RDYQDENLEEAVQALGDLIRVGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPL 172 Query: 202 --QVIFNIFRQKPLADLLPQAKAKGVGIIVRLPLASG 236 Q +N+ ++P ++LP G+G++ P+A G Sbjct: 173 VCQPYYNLLNRQPEVEILPACAHYGLGVVPYSPIARG 209 >gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 69.3 bits (168), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 59/204 (28%), Positives = 96/204 (47%), Gaps = 20/204 (9%) Query: 40 RDGLTVPEVGLGCWQLGGGWGDHWDNDVAQQTLAQAYATGVRFIDTADVYGDGASERSIG 99 R+G +VP +G G W +G + + L G+ IDTA++Y +G +ER + Sbjct: 10 RNGQSVPILGQGTWNMGESPAQ---ANAEVRALQAGLDLGMSLIDTAEMYAEGGAERIVA 66 Query: 100 QFLTSHKD-LTVATKLGRGAIYPDGYSREAVRTATQRSLERLGVDKLDLTQLH---CVPP 155 + + +D + + +K + P S+ A + SL+RLG D++DL LH P Sbjct: 67 KAIAGRRDQVYLVSK-----VLPHNASKRGTIAACEASLKRLGTDRIDLYLLHWRGAHPL 121 Query: 156 AVLKDGKIFDWMRELRDDGLITRFGAS-VETVEEGLLCLEQEGMTSL--QVIFNIFRQKP 212 A +G M L G I +G S +T + L E G L QV++N+ R+ Sbjct: 122 AATIEG-----METLVSQGKIGGWGVSNFDTDDIDDLLEEPHGEHYLCNQVLYNLSRRGI 176 Query: 213 LADLLPQAKAKGVGIIVRLPLASG 236 DLLPQA+ + ++ P+ G Sbjct: 177 EYDLLPQAQQAHIAVMAYSPIEQG 200 >gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 344 Score = 60.5 bits (145), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 60/221 (27%), Positives = 101/221 (45%), Gaps = 22/221 (9%) Query: 33 MQTRKLARDGLTVPEVGLGCWQLGGGWGDHWDNDVAQQTLAQAYATGVRFIDTADVYGD- 91 M R+ R GL +P + LG WQ GG + + + L +A+ GV D A+ YG Sbjct: 14 MPYRRTGRSGLLLPAISLGLWQNFGGVDNF---ETGRAVLRRAFDRGVTHFDLANNYGPP 70 Query: 92 -GASERSIGQ-----FLTSHKDLTVATKLGRGAIYPDGY-----SREAVRTATQRSLERL 140 G++E + G+ FL +L +++K G ++P Y +R+ + + ++SL+R+ Sbjct: 71 AGSAEENFGRWMAKDFLPYRDELVISSKAG-WQMWPGPYGGPSGARKHLIASCEQSLKRM 129 Query: 141 GVDKLDLTQLHCVPPAVLKDGKIFDWMRELRDDGLITRFGASV---ETVEEGLLCLEQEG 197 G+D +D+ H V + + + L G G S E + L+ EG Sbjct: 130 GLDYVDIFYSHRVDADTPLE-ETMGALAHLHRQGKALYVGISSYSPELTRKAAAILKSEG 188 Query: 198 MTSL--QVIFNIFRQKPLADLLPQAKAKGVGIIVRLPLASG 236 + L Q +++ + LLP + GVG I PLA G Sbjct: 189 VPLLIHQPNYSMLNRAIEGGLLPALEELGVGCIGFSPLAQG 229 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 718,819 Number of extensions: 28034 Number of successful extensions: 96 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 89 Number of HSP's successfully gapped: 5 Length of query: 239 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 157 Effective length of database: 1,231,600 Effective search space: 193361200 Effective search space used: 193361200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)